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Disease biomarkers

Section edited by Xiangdong Wang

This section aims to bridge the identification and validation of discovered gene or protein-based, network or dynamic network biomarkers with human diseases, patient phenotypes, or clinical applications, and accelerate the development of human disease-specific biomarkers for the early diagnosis, monitoring, evaluation, and prediction of diseases. Conditions including cancers, cardiovascular and metabolic diseases will be the focus of study. This section will promote the innovation and development of disease-specific biomarkers by integrating multidisciplinary aspects of science.

Studies considered for publication include those regarding the development of advanced biotechnologies for biomarker discovery and development, identifying biomarkers associated with the early detection of diseases, monitoring of disease severity and duration as well as patient responses to therapies, prediction of patient outcomes and the evaluation, regulation and ethics of disease biomarkers.

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  1. Content type: Research

    While prostate cancer can often manifest as an indolent disease, the development of locally-advanced or metastatic disease can cause significant morbidity or mortality. Elucidation of molecular mechanisms cont...

    Authors: Aruz Mesci, Fabrice Lucien, Xiaoyong Huang, Eric H. Wang, David Shin, Michelle Meringer, Christianne Hoey, Jessica Ray, Paul C. Boutros, Hon S. Leong and Stanley K. Liu

    Citation: Journal of Translational Medicine 2019 17:125

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  2. Content type: Research

    Noninvasive prenatal screening (NIPS) based on cell-free fetal DNA (cffDNA) has rapidly been applied into clinic. However, the reliability of this method largely depends on the concentration of cffDNA in the m...

    Authors: Ping Hu, Dong Liang, Yangyi Chen, Ying Lin, Fengchang Qiao, Hang Li, Ting Wang, Chunfang Peng, Donghong Luo, Hailiang Liu and Zhengfeng Xu

    Citation: Journal of Translational Medicine 2019 17:124

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  3. Content type: Research

    Triple negative breast cancer (TNBC) has been classified as a disease subgroup defined by the lack of expression of estrogen and progesterone receptors as well as the absence of the human epidermal growth fact...

    Authors: Wijden Mahfoudh, Inchirah Bettaieb, Randa Ghedira, Kaouther Snoussi, Nadia Bouzid, Zahra Klayech, Sallouha Gabbouj, Yassmine Remadi, Elham Hassen, Noureddine Bouaouina and Abdelfateh Zakhama

    Citation: Journal of Translational Medicine 2019 17:123

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  4. Content type: Research

    Previous studies have shown that zinc-finger CCHC-type containing 13 (ZCCHC13) is located in an imprinted gene cluster in the X-inactivation centre, but few published studies have provided evidence of its expr...

    Authors: Zhiming Li, Zhi Li, Linjun Wang, Chen Long, Zaozao Zheng and Xuan Zhuang

    Citation: Journal of Translational Medicine 2019 17:108

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  5. Content type: Research

    Acute myeloid leukemia (AML) is a highly heterogeneous malignancy with various outcomes, and therefore needs better risk stratification tools to help select optimal therapeutic options.

    Authors: Huihui Zhang, Ninghan Zhang, Rong Wang, Tingting Shao, Yuan Feng, Yao Yao, Qingyun Wu, Shengyun Zhu, Jiang Cao, Huanxin Zhang, Zhenyu Li, Xuejiao Liu, Mingshan Niu and Kailin Xu

    Citation: Journal of Translational Medicine 2019 17:106

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  6. Content type: Research

    Leukemia stem cell (LSC)-enriched genes have been shown to be highly prognostic in acute myeloid leukemia (AML). However, the prognostic value of tumor suppressor genes (TSGs) that are repressed early in LSC r...

    Authors: Zi-jun Xu, Ji-chun Ma, Jing-dong Zhou, Xiang-mei Wen, Dong-ming Yao, Wei Zhang, Run-bi Ji, De-hong Wu, Li-juan Tang, Zhao-qun Deng, Jun Qian and Jiang Lin

    Citation: Journal of Translational Medicine 2019 17:102

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  7. Content type: Research

    Oral squamous cell carcinoma (OSCC) is a common oral and maxillofacial malignant tumor with high rates of metastasis and mortality. Circular RNAs (circRNAs), a type of non-coding RNA, are involved in the devel...

    Authors: Wen Su, Shuai Sun, Feng Wang, Yuehong Shen and Hongyu Yang

    Citation: Journal of Translational Medicine 2019 17:76

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  8. Content type: Methodology

    Extracellular vesicles (EVs) are small membrane-bound vesicles which play an important role in cell-to-cell communication. Their molecular cargo analysis is presented as a new source for biomarker detection, a...

    Authors: N. García-Romero, R. Madurga, G. Rackov, I. Palacín-Aliana, R. Núñez-Torres, A. Asensi-Puig, J. Carrión-Navarro, S. Esteban-Rubio, H. Peinado, A. González-Neira, V. González-Rumayor, C. Belda-Iniesta and A. Ayuso-Sacido

    Citation: Journal of Translational Medicine 2019 17:75

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  9. Content type: Research

    CEA, CYFRA21-1 and NSE are tumor markers used for monitoring the response to chemotherapy in advanced adenocarcinoma, squamous cell carcinoma and small-cell lung cancer, respectively. Their role in cancer immu...

    Authors: M. G. Dal Bello, R. A. Filiberti, A. Alama, A. M. Orengo, M. Mussap, S. Coco, I. Vanni, S. Boccardo, E. Rijavec, C. Genova, F. Biello, G. Barletta, G. Rossi, M. Tagliamento, C. Maggioni and F. Grossi

    Citation: Journal of Translational Medicine 2019 17:74

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  10. Content type: Research

    Currently, pathological examination of gastroscopy biopsy specimens is the gold standard for gastric cancer (GC) diagnosis. However, it has a false-negative rate of 10–20% due to inaccurate sampling locations ...

    Authors: Haidan Yan, Meifeng Li, Longlong Cao, Haifeng Chen, Hungming Lai, Qingzhou Guan, Huxing Chen, Wenbin Zhou, Baotong Zheng, Zheng Guo and Chaohui Zheng

    Citation: Journal of Translational Medicine 2019 17:63

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  11. Content type: Research

    Diabetic kidney disease (DKD) is the leading cause of end-stage kidney disease (ESKD) in the world. Emerging evidence has shown that urinary mRNAs may serve as early diagnostic and prognostic biomarkers of DKD...

    Authors: Le-Ting Zhou, Lin-Li Lv, Shen Qiu, Qing Yin, Zuo-Lin Li, Tao-Tao Tang, Li-Hua Ni, Ye Feng, Bin Wang, Kun-Ling Ma and Bi-Cheng Liu

    Citation: Journal of Translational Medicine 2019 17:59

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  12. Content type: Research

    Inflammation and demyelination are the main processes in multiple sclerosis. Nevertheless, to date, blood biomarkers of inflammation are lacking. TWEAK, a transmembrane protein that belongs to the TNF ligand f...

    Authors: Adil Maarouf, Delphine Stephan, Marie-Pierre Ranjeva, Jean-Philippe Ranjeva, Jean Pelletier, Bertrand Audoin, Michel Khrestchatisky and Sophie Desplat-Jégo

    Citation: Journal of Translational Medicine 2019 17:51

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  13. Content type: Research

    Recently, circular RNAs (circRNAs) have been reported to be microRNA sponges and play essential roles in cancer development. This study aimed to evaluate whether circulating circRNAs could be used as diagnosti...

    Authors: Xiao-Xia Liu, Yi-E Yang, Xiao Liu, Meng-Yu Zhang, Rui Li, Yun-Hong Yin and Yi-Qing Qu

    Citation: Journal of Translational Medicine 2019 17:50

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  14. Content type: Research

    Although previous prevalence studies of DED were reported from some countries worldwide, national data are unavailable in China. We aimed to conduct an up-to-date national survey on the prevalence of DED in Ch...

    Authors: Donghui Yu, Qinglong Deng, Jiwei Wang, Xing Chang, Shuxiao Wang, Renren Yang, Jinming Yu and Jing Yu

    Citation: Journal of Translational Medicine 2019 17:46

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  15. Content type: Research

    Circulating tumor cells (CTC) shows great prospect to realize precision medicine in cancer patients.

    Authors: Weiling He, Di Xu, Zhuo Wang, Hui Wu, Xianhong Xiang, Bing Tang, Wenting Jiang, Yongmei Cui, Han Wang, Neng Jiang, Yu Sun, Yangshan Chen, Shuhua Li, Minzhi Hou, Yang Zhang, Liantang Wang…

    Citation: Journal of Translational Medicine 2019 17:32

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  16. Content type: Review

    Accumulated evidence has demonstrated that WNT1 inducible signaling pathway protein (WISP) genes, which belong to members of the CCN growth factor family, play a pivotal role in tumorigenesis and progression o...

    Authors: Yi Liu, Yizuo Song, Miaomiao Ye, Xiaoli Hu, Z. Peter Wang and Xueqiong Zhu

    Citation: Journal of Translational Medicine 2019 17:28

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  17. Content type: Research

    Despite much progress in cancer research, its incidence and mortality continue to rise. A robust biomarker that would predict tumor behavior is highly desirable and could improve patient treatment and prognosis.

    Authors: Wai Hoong Chang, Donall Forde and Alvina G. Lai

    Citation: Journal of Translational Medicine 2019 17:14

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  18. Content type: Methodology

    Hepatitis B virus (HBV) infections represent a global health problem and chronic hepatitis B (CHB) leads to liver cirrhosis and hepatocellular carcinoma. Thus, timely diagnosis of hepatitis B is crucial to ens...

    Authors: Katharina Bröker, Robin Terzenbach, Frank Bentzien, Stefan Lüth and Werner Dammermann

    Citation: Journal of Translational Medicine 2019 17:6

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  19. Content type: Research

    Neoadjuvant chemotherapy (NAC) has become the standard of care for resectable esophageal squamous cell carcinoma (ESCC) which is one of the most lethal cancers, to improve resectability and prognosis. On this ...

    Authors: Yukiko Niwa, Suguru Yamada, Fuminori Sonohara, Keisuke Kurimoto, Masamichi Hayashi, Mitsuru Tashiro, Naoki Iwata, Mitsuro Kanda, Chie Tanaka, Daisuke Kobayashi, Goro Nakayama, Masahiko Koike, Michitaka Fujiwara and Yasuhiro Kodera

    Citation: Journal of Translational Medicine 2019 17:1

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  20. Content type: Research

    Lung adenocarcinoma (LUAD), largely remains a primary cause of cancer-related death worldwide. The molecular mechanisms in LUAD metastasis have not been completely uncovered.

    Authors: Lifeng Li, Mengle Peng, Wenhua Xue, Zhirui Fan, Tian Wang, Jingyao Lian, Yunkai Zhai, Wenping Lian, Dongchun Qin and Jie Zhao

    Citation: Journal of Translational Medicine 2018 16:372

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  21. Content type: Research

    Systemic lupus erythematosus (SLE) is a multisystemic autoimmune disease with various clinical manifestations. MicroRNAs (miRNAs) and immunometabolism are recognized as key elements in SLE pathogenesis; howeve...

    Authors: Gangqiang Guo, Huijing Wang, Xinyu Shi, Lele Ye, Kai Wu, Kangmin Lin, Sisi Ye, Baoqing Li, Huidi Zhang, Qiaoai Lin, Shuang Ye, Xiangyang Xue and Chaosheng Chen

    Citation: Journal of Translational Medicine 2018 16:370

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  22. Content type: Research

    Adenosine-to-inosine (A-to-I) RNA editing is catalyzed by adenosine deaminases acting on RNA (ADAR) enzymes. Recent evidence suggests that RNA editing of antizyme inhibitor 1 (AZIN1) RNA is emerging as a key epig...

    Authors: Yoshinaga Okugawa, Yuji Toiyama, Kunitoshi Shigeyasu, Akira Yamamoto, Tsunehiko Shigemori, Chengzeng Yin, Takashi Ichikawa, Hiromi Yasuda, Hiroyuki Fujikawa, Shigeyuki Yoshiyama, Junichiro Hiro, Masaki Ohi, Toshimitsu Araki, Masato Kusunoki and Ajay Goel

    Citation: Journal of Translational Medicine 2018 16:366

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  23. Content type: Research

    Immunoglobulin A (IgA) and IgG are major components in human intestinal mucosal surface and sera, and IgA- or IgG-coated bacteria play a vital role in the intestinal homeostasis. However, the correlation of Ig...

    Authors: Ritian Lin, Hongwei Chen, Weigang Shu, Mingming Sun, Leilei Fang, Yanhong Shi, Zhi Pang, Wei Wu and Zhanju Liu

    Citation: Journal of Translational Medicine 2018 16:359

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  24. Content type: Research

    The androgen receptor (AR) is expressed in the majority of breast cancers and across the main breast cancer subtypes. Despite the high frequency of AR expression in breast cancer its appraisal remains controve...

    Authors: Monika Pizon, Daniel Lux, Ulrich Pachmann, Katharina Pachmann and Dorothea Schott

    Citation: Journal of Translational Medicine 2018 16:356

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  25. Content type: Research

    Increasing evidences showed that the location of the primary tumor on the right (proximal) or left (distal) side of the colon have a prognostic/predictive value for colon cancer patients. However, the understa...

    Authors: Fabiana Tatangelo, Annabella Di Mauro, Giosuè Scognamiglio, Gabriella Aquino, Antonio Lettiero, Paolo Delrio, Antonio Avallone, Monica Cantile and Gerardo Botti

    Citation: Journal of Translational Medicine 2018 16:350

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  26. Content type: Research

    In view of the limited knowledge of plasma biomarkers relating to cancer resistance to radiotherapy, we have set up screening, training and testing stages to investigate the microRNAs (miRNAs) expression profi...

    Authors: An-Lun Li, Tao-Sang Chung, Yao-Ning Chan, Chien-Lung Chen, Shih-Chieh Lin, Yun-Ru Chiang, Chen-Huan Lin, Chi-Ching Chen and Nianhan Ma

    Citation: Journal of Translational Medicine 2018 16:341

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  27. Content type: Research

    Hepatocellular carcinoma is a malignant tumor with a highly invasive and metastatic phenotype, and the detection of potential indicators associated with its recurrence and metastasis after surgical resection i...

    Authors: Zheng Yu, Qifeng Ou, Fan Chen, Jiong Bi, Wen Li, Jieyi Ma, Rongchang Wang and Xiaohui Huang

    Citation: Journal of Translational Medicine 2018 16:327

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  28. Content type: Research

    Chronic obstructive pulmonary disease (COPD) is a complex condition, whose diagnosis requires spirometric assessment. However, considering its heterogeneity, subjects with similar spirometric parameters do not...

    Authors: Tamara Pavić, Dario Dilber, Domagoj Kifer, Najda Selak, Toma Keser, Đivo Ljubičić, Andrea Vukić Dugac, Gordan Lauc, Lada Rumora and Olga Gornik

    Citation: Journal of Translational Medicine 2018 16:323

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  29. Content type: Research

    Myalgic encephalomyelitis (ME) is a complex and debilitating disease that often initially presents with flu-like symptoms, accompanied by incapacitating fatigue. Currently, there are no objective biomarkers or...

    Authors: Kenny L. De Meirleir, Tatjana Mijatovic, Krishnamurthy Subramanian, Karen A. Schlauch and Vincent C. Lombardi

    Citation: Journal of Translational Medicine 2018 16:322

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  30. Content type: Research

    The nucleotide oligomerization domain-like receptor subfamily C5 (NLRC5) is primarily expressed in the adaptive and innate immune systems. NLRC5 was recently discovered to regulate immunity and inflammatory re...

    Authors: Yusa Chen, Huihui Li, Chenggen Xiao, Xiangli Zeng, Xiangcheng Xiao, Qiaoling Zhou and Ping Xiao

    Citation: Journal of Translational Medicine 2018 16:317

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  31. Content type: Research

    Uromodulin is specifically synthesized and secreted by kidney tubular epithelial cells. Studies on the association of serum uromodulin and outcomes of chronic kidney disease (CKD) are lacking. This study aimed...

    Authors: Li Lv, Jinwei Wang, Bixia Gao, Liang Wu, Fang Wang, Zhao Cui, Kevin He, Luxia Zhang, Min Chen and Ming-Hui Zhao

    Citation: Journal of Translational Medicine 2018 16:316

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  32. Content type: Research

    Brain metastasis is an extremely serious sequela with a dismal prognosis in non-small cell lung cancer (NSCLC). The present study aimed to identify novel biomarkers and potential therapeutic targets for brain ...

    Authors: Fanrong Zhang, Lisha Ying, Jiaoyue Jin, Jianguo Feng, Kaiyan Chen, Minran Huang, Yingxue Wu, Herbert Yu and Dan Su

    Citation: Journal of Translational Medicine 2018 16:310

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  33. Content type: Research

    Sarcomas are challenging to study because of their rarity and histomorphological complexity. PD1 and PD-L1 inhibitors showed a promising anti-tumor effect in solid tumors, where a relationship between PD-L1 ex...

    Authors: Hyung Kyu Park, Mingi Kim, Minjung Sung, Seung Eun Lee, Yu Jin Kim and Yoon-La Choi

    Citation: Journal of Translational Medicine 2018 16:303

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  34. Content type: Research

    It was recently demonstrated that the size of cell-free DNA (cfDNA) fragments that originates from tumor cells are shorter than cfDNA fragments that originates from non-malignant cells. We investigated whether...

    Authors: Morten Lapin, Satu Oltedal, Kjersti Tjensvoll, Tove Buhl, Rune Smaaland, Herish Garresori, Milind Javle, Nils Idar Glenjen, Bente Kristin Abelseth, Bjørnar Gilje and Oddmund Nordgård

    Citation: Journal of Translational Medicine 2018 16:300

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  35. Content type: Research

    Human saliva is a protein-rich, easily accessible source of potential biomarkers for the diagnosis of oral and systemic diseases. However, little is known about the changes in salivary proteome associated with...

    Authors: Kun Wang, Xiuqing Wang, Sainan Zheng, Yumei Niu, Wenyue Zheng, Xi Qin, Zhongcheng Li, Junyuan Luo, Wentao Jiang, Xuedong Zhou, Wei Li and Linglin Zhang

    Citation: Journal of Translational Medicine 2018 16:293

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  36. Content type: Review

    Chronic kidney disease (CKD) is recognised as a global public health problem, more prevalent in older persons and associated with multiple co-morbidities. Diabetes mellitus and hypertension are common aetiolog...

    Authors: M. Cañadas-Garre, K. Anderson, J. McGoldrick, A. P. Maxwell and A. J. McKnight

    Citation: Journal of Translational Medicine 2018 16:292

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  37. Content type: Research

    Epidemiological evidence suggests sex difference in serum uric acid (SUA) and alanine aminotransferase (ALT) might be a potential explanation for the gender difference in prevalence of non-alcoholic fatty live...

    Authors: Huanhuan Yang, Deming Li, Xiaochao Song, Fang Liu, Xinjing Wang, Qinghua Ma, Xi Zhang and Xinli Li

    Citation: Journal of Translational Medicine 2018 16:285

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  38. Content type: Methodology

    Molecular and cellular pathophysiological events occurring in the majority of rare kidney diseases remain to be elucidated. Familial hypomagnesemia with hypercalciuria and nephrocalcinosis (FHHNC) is a rare au...

    Authors: M. Vall-Palomar, J. Arévalo, G. Ariceta and A. Meseguer

    Citation: Journal of Translational Medicine 2018 16:278

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  39. Content type: Research

    Systemic inflammation and immune dysfunction has been proved to be significantly associated with cancer progression and metastasis in many cancer types, including colorectal cancer. We examined the prognostic ...

    Authors: Qian-Kun Xie, Ping Chen, Wan-Ming Hu, Peng Sun, Wen-Zhuo He, Chang Jiang, Peng-Fei Kong, Shou-Sheng Liu, Hai-Tian Chen, Yuan-Zhong Yang, Dan Wang, Lin Yang and Liang-Ping Xia

    Citation: Journal of Translational Medicine 2018 16:273

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  40. Content type: Research

    In the recent years Plasmodium vivax has been reported to cause severe infections associated with mortality. Clinical evaluation has limited accuracy for the early identification of the patients progressing towar...

    Authors: Hargobinder Kaur, Rakesh Sehgal, Archit Kumar, Alka Sehgal, Devendra Bansal and Ali A. Sultan

    Citation: Journal of Translational Medicine 2018 16:272

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  41. Content type: Research

    Limited evidence suggests that inherited predisposing risk variants might affect the disease outcome. In this study, we analyzed the effect of genome-wide association studies—identified breast cancer-risk sing...

    Authors: Fangmeng Fu, Wenhui Guo, Yuxiang Lin, Bangwei Zeng, Wei Qiu, Meng Huang and Chuan Wang

    Citation: Journal of Translational Medicine 2018 16:270

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  42. Content type: Review

    Myalgic encephalomyelitis/chronic fatigue syndrome (ME/CFS) is a complex and controversial clinical condition without having established causative factors. Increasing numbers of cases during past decade have c...

    Authors: Santa Rasa, Zaiga Nora-Krukle, Nina Henning, Eva Eliassen, Evelina Shikova, Thomas Harrer, Carmen Scheibenbogen, Modra Murovska and Bhupesh K. Prusty

    Citation: Journal of Translational Medicine 2018 16:268

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  43. Content type: Research

    Substantial evidence indicates that β-catenin is a pivotal regulator that contributes to the initiation and development of various types of diseases. Recently, β-catenin can be detected in human serum and also...

    Authors: Liang Duan, Qianfan Yang, Jun Yang, Qin Hu, Bo Wang, Pu Li and Weixian Chen

    Citation: Journal of Translational Medicine 2018 16:265

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  44. Content type: Research

    Increasing evidence has underscored the role of long non-coding RNAs (lncRNAs) acting as competing endogenous RNAs (ceRNAs) in the development and progression of tumors. Nevertheless, lncRNA biomarkers in lncR...

    Authors: Chun-Ni Fan, Lei Ma and Ning Liu

    Citation: Journal of Translational Medicine 2018 16:264

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  45. Content type: Review

    The airway smooth muscle (ASM) plays an indispensable role in airway structure and function. Dysfunction in ASM plays a central role in the pathogenesis of chronic obstructive pulmonary disease (COPD) and cont...

    Authors: Furong Yan, Hongzhi Gao, Hong Zhao, Madhav Bhatia and Yiming Zeng

    Citation: Journal of Translational Medicine 2018 16:262

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  46. Content type: Research

    MUC4 is a membrane-bound mucin that promotes carcinogenetic progression and is often proposed as a promising biomarker for various carcinomas. In this manuscript, we analyzed large scale genomic datasets in or...

    Authors: Nicolas Jonckheere and Isabelle Van Seuningen

    Citation: Journal of Translational Medicine 2018 16:259

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  47. Content type: Research

    Even though multi-focused psychosocial residence rehabilitation intervention (MPRRI) programs are widely implemented by the Shanghai Cancer Rehabilitation Club, these programs have not been rigorously evaluate...

    Authors: Xuefen Chen, Xiaohuan Gong, Changhong Shi, Li Sun, Zheng Tang, Zhengping Yuan, Jiwei Wang and Jinming Yu

    Citation: Journal of Translational Medicine 2018 16:250

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  48. Content type: Research

    Metabolomics represent a valuable tool to recover biological information using body fluids and may help to characterize pathophysiological mechanisms of the studied disease. This approach has not been widely u...

    Authors: Abdellah Tebani, Lenaig Abily-Donval, Isabelle Schmitz-Afonso, Bénédicte Héron, Monique Piraud, Jérôme Ausseil, Farid Zerimech, Bruno Gonzalez, Stéphane Marret, Carlos Afonso and Soumeya Bekri

    Citation: Journal of Translational Medicine 2018 16:248

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  49. Content type: Commentary

    We have previously reported that nuclear expression of the Hippo transducer TAZ in association with Wnt pathway mutations negatively impacts survival outcomes in advanced gastric cancer (GC) patients. Here, we...

    Authors: Matteo Pallocca, Frauke Goeman, Francesca De Nicola, Elisa Melucci, Francesca Sperati, Irene Terrenato, Laura Pizzuti, Beatrice Casini, Enzo Gallo, Carla Azzurra Amoreo, Patrizia Vici, Luigi Di Lauro, Simonetta Buglioni, Maria Grazia Diodoro, Edoardo Pescarmona, Marco Mazzotta…

    Citation: Journal of Translational Medicine 2018 16:247

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  50. Content type: Research

    Alternative N-glycosylation has significant structural and functional consequences on immunoglobulin G (IgG) and can affect immune responses, acting as a switch between pro- and anti-inflammatory IgG functionalit...

    Authors: Di Liu, Xi Chu, Hao Wang, Jing Dong, Si-Qi Ge, Zhong-Yao Zhao, Hong-Li Peng, Ming Sun, Li-Juan Wu, Man-Shu Song, Xiu-Hua Guo, Qun Meng, You-Xin Wang, Gordan Lauc and Wei Wang

    Citation: Journal of Translational Medicine 2018 16:235

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