Articles

Integrative analysis of the cancer genome atlas and cancer cell lines encyclopedia large-scale genomic databases: MUC4/MUC16/MUC20 signature is associated with poor survival in human carcinomas

MUC4 is a membrane-bound mucin that promotes carcinogenetic progression and is often proposed as a promising biomarker for various carcinomas. In this manuscript, we analyzed large scale genomic datasets in or...

Authors: Nicolas Jonckheere and Isabelle Van Seuningen

The role of diffusion tensor imaging as an objective tool for the assessment of motor function recovery after paraplegia in a naturally-occurring large animal model of spinal cord injury

Traumatic spinal cord injury (SCI) results in sensory and motor function impairment and may cause a substantial social and economic burden. For the implementation of novel treatment strategies, parallel develo...

Authors: Adriano Wang-Leandro, Marc K. Hobert, Sabine Kramer, Karl Rohn, Veronika M. Stein and Andrea Tipold

The diagnostic value of [¹⁸F]-FDG-PET/CT in assessment of radiation renal injury in Tibet minipigs model

Radiation-induced kidney damage can severely affect renal function, and have a serious impact on glucose reabsorption. Fluoro-2-deoxyglucose positron emission tomography (FDG-PET) is routinely utilized for met...

Authors: Yu-Guang Tian, Min Yue, Bayaer Nashun, Shao-Jie Wu, Wei-Wang Gu and Yu-Jue Wang

Proceedings of 2nd Annual Charles River World Congress: Lessons Learned from Rare Disease and Personalized Medicine Approaches

Authors:

MAGIC: once upon a time in consent management—a FHIR^® tale

The use of medical data for research purposes requires an informed consent of the patient that is compliant with the EU General Data Protection Regulation. In the context of multi-centre research initiatives a...

Authors: Martin Bialke, Thomas Bahls, Lars Geidel, Henriette Rau, Arne Blumentritt, Sandra Pasewald, Robert Wolff, Jonas Steinmann, Tobias Bronsch, Björn Bergh, Galina Tremper, Martin Lablans, Frank Ückert, Stefan Lang, Tarik Idris and Wolfgang Hoffmann

Differential responses of MET activations to MET kinase inhibitor and neutralizing antibody

Aberrant MET tyrosine kinase signaling is known to cause cancer initiation and progression. While MET inhibitors are in clinical trials against several cancer types, the clinical efficacies are controversial a...

Authors: Jianqun Kou, Phillip R. Musich, Ben Staal, Liang Kang, Yuan Qin, Zhi Q. Yao, Boheng Zhang, Weizhong Wu, Angela Tam, Alan Huang, Huai-Xiang Hao, George F. Vande Woude and Qian Xie

Sex-specific associations between telomere length and candidate miRNA expression in placenta

In the early-life environment, proper development of the placenta is essential for both fetal and maternal health. Telomere length at birth has been related to life expectancy. MicroRNAs (miRNAs) as potential ...

Authors: Maria Tsamou, Dries S. Martens, Bianca Cox, Narjes Madhloum, Karen Vrijens and Tim S. Nawrot

Increased circulating endothelial progenitor cells and improved short-term outcomes in acute non-cardioembolic stroke after hyperbaric oxygen therapy

Acute ischemic stroke is a leading cause of mortality and long-term disability, and profiles of endothelial progenitor cells (EPCs) reflect the degree of endothelial impairment. This study tested the hypothesi...

Authors: Chen-Yu Chen, Re-Wen Wu, Nai-Wen Tsai, Mel S. Lee, Wei-Che Lin, Mei-Chi Hsu, Chih-Cheng Huang, Yun-Ru Lai, Chia-Te Kung, Hung-Chen Wang, Yu-Jih Su, Chih-Min Su, Sheng-Yuan Hsiao, Ben-Chung Cheng, Yi-Fang Chiang and Cheng-Hsien Lu

Multi-focused psychosocial residential rehabilitation interventions improve quality of life among cancer survivors: a community-based controlled trial

Even though multi-focused psychosocial residence rehabilitation intervention (MPRRI) programs are widely implemented by the Shanghai Cancer Rehabilitation Club, these programs have not been rigorously evaluate...

Authors: Xuefen Chen, Xiaohuan Gong, Changhong Shi, Li Sun, Zheng Tang, Zhengping Yuan, Jiwei Wang and Jinming Yu

Circulating tumor cells criteria (CyCAR) versus standard RECIST criteria for treatment response assessment in metastatic colorectal cancer patients

The use of circulating tumor cells (CTCs) as indicators of treatment response in metastatic colorectal cancer (mCRC) needs to be clarified. The objective of this study is to compare the Response Evaluation Cri...

Authors: Mayte Delgado-Ureña, Francisco G. Ortega, Diego de Miguel-Pérez, Alba Rodriguez-Martínez, Jose L. García-Puche, Hugh Ilyine, Jose A. Lorente, Jose Exposito-Hernandez, M. Carmen Garrido-Navas, Miguel Delgado-Ramirez and M. José Serrano

Unveiling metabolic remodeling in mucopolysaccharidosis type III through integrative metabolomics and pathway analysis

Metabolomics represent a valuable tool to recover biological information using body fluids and may help to characterize pathophysiological mechanisms of the studied disease. This approach has not been widely u...

Authors: Abdellah Tebani, Lenaig Abily-Donval, Isabelle Schmitz-Afonso, Bénédicte Héron, Monique Piraud, Jérôme Ausseil, Farid Zerimech, Bruno Gonzalez, Stéphane Marret, Carlos Afonso and Soumeya Bekri

CCR5del32 genotype in human enteroviral cardiomyopathy leads to spontaneous virus clearance and improved outcome compared to wildtype CCR5

Enteroviral cardiomyopathy is a life-threatening disease, and detection of enterovirus (EV) RNA in the initial endomyocardial biopsy is associated with adverse prognosis and increased mortality. Some patients ...

Authors: Dirk Lassner, Christine S. Siegismund, Uwe Kühl, Maria Rohde, Andrea Stroux, Felicitas Escher and Heinz-Peter Schultheiss

Coexisting YAP expression and TP53 missense mutations delineates a molecular scenario unexpectedly associated with better survival outcomes in advanced gastric cancer

We have previously reported that nuclear expression of the Hippo transducer TAZ in association with Wnt pathway mutations negatively impacts survival outcomes in advanced gastric cancer (GC) patients. Here, we...

Authors: Matteo Pallocca, Frauke Goeman, Francesca De Nicola, Elisa Melucci, Francesca Sperati, Irene Terrenato, Laura Pizzuti, Beatrice Casini, Enzo Gallo, Carla Azzurra Amoreo, Patrizia Vici, Luigi Di Lauro, Simonetta Buglioni, Maria Grazia Diodoro, Edoardo Pescarmona, Marco Mazzotta…

Symptomatic anterior cruciate ligament tears treated with percutaneous injection of autologous bone marrow concentrate and platelet products: a non-controlled registry study

Bone marrow concentrate (BMC) has shown promise in the treatment of several orthopedic conditions. This registry study investigated the use of autologous BMC and platelet products for percutaneous anterior cru...

Authors: Christopher Centeno, Jason Markle, Ehren Dodson, Ian Stemper, Christopher Williams, Matthew Hyzy, Thomas Ichim and Michael Freeman

Fecal microbiota and bile acid interactions with systemic and adipose tissue metabolism in diet-induced weight loss of obese postmenopausal women

Microbiota and bile acids in the gastrointestinal tract profoundly alter systemic metabolic processes. In obese subjects, gradual weight loss ameliorates adipose tissue inflammation and related systemic change...

Authors: José O. Alemán, Nicholas A. Bokulich, Jonathan R. Swann, Jeanne M. Walker, Joel Correa De Rosa, Thomas Battaglia, Adele Costabile, Alexandros Pechlivanis, Yupu Liang, Jan L. Breslow, Martin J. Blaser and Peter R. Holt

Effect of metformin on the survival of patients with ALL who express high levels of the ABCB1 drug resistance gene

In acute lymphoblastic leukemia (ALL), high ABCB1 gene expression has been associated with treatment resistance, which affects patient prognosis. Many preclinical reports and retrospective population studies h...

Authors: Christian Ramos-Peñafiel, Irma Olarte-Carrillo, Rafael Cerón-Maldonado, Etta Rozen-Fuller, Juan Julio Kassack-Ipiña, Guillermo Meléndez-Mier, Juan Collazo-Jaloma and Adolfo Martínez-Tovar

Sulfated non-anticoagulant heparin blocks Th2-induced asthma by modulating the IL-4/signal transducer and activator of transcription 6/Janus kinase 1 pathway

The efficacy of heparins and low-MW-heparins (LMWH) against human asthma has been known for decades. However, the clinical utility of these compounds has been hampered by their anticoagulant properties. Much e...

Authors: Mohamed A. Ghonim, Jeffrey Wang, Salome V. Ibba, Hanh H. Luu, Kusma Pyakurel, Ilyes Benslimane, Shaker Mousa and A. Hamid Boulares

Unleashing endogenous TNF-alpha as a cancer immunotherapeutic

Tumor necrosis factor (TNF)-alpha was originally identified in the 1970s as the serum mediator of innate immunity capable of inducing hemorrhagic necrosis in tumors. Today, a wide spectrum of biological activi...

Authors: Steven F. Josephs, Thomas E. Ichim, Stephen M. Prince, Santosh Kesari, Francesco M. Marincola, Anton Rolando Escobedo and Amir Jafri

Acute effects of different resistance training loads on cardiac autonomic modulation in hypertensive postmenopausal women

Individuals with arterial hypertension often have an autonomic nervous system (ANS) imbalance with predominance of sympathetic ANS. This predominance can lead to injury of several organs affecting its function...

Authors: Arthur F. Vale, Juliana A. Carneiro, Paulo C. V. Jardim, Thiago V. Jardim, James Steele, James P. Fisher and Paulo Gentil

Molecular analysis of inherited cardiomyopathy using next generation semiconductor sequencing technologies

Cardiomyopathies are the most common clinical and genetic heterogeneity cardiac diseases, and genetic contribution in particular plays a major role in patients with primary cardiomyopathies. The aim of this st...

Authors: Chaoxia Lu, Wei Wu, Fang Liu, Kunqi Yang, Jiacheng Li, Yaping Liu, Rongrong Wang, Nuo Si, Peng Gao, Yongtai Liu, Shuyang Zhang and Xue Zhang

Invasive non-typhoidal Salmonella in sickle cell disease in Africa: is increased gut permeability the missing link?

Non-typhoidal Salmonella usually induces self-limiting gastroenteritis. However, in many parts of Africa, especially in individuals who are malnourished, infected with malaria, or have sickle cell disease, the...

Authors: Seah H. Lim, Barbara A. Methé, Bettina M. Knoll, Alison Morris and Stephen K. Obaro

The changes of immunoglobulin G N-glycosylation in blood lipids and dyslipidaemia

Alternative N-glycosylation has significant structural and functional consequences on immunoglobulin G (IgG) and can affect immune responses, acting as a switch between pro- and anti-inflammatory IgG functionalit...

Authors: Di Liu, Xi Chu, Hao Wang, Jing Dong, Si-Qi Ge, Zhong-Yao Zhao, Hong-Li Peng, Ming Sun, Li-Juan Wu, Man-Shu Song, Xiu-Hua Guo, Qun Meng, You-Xin Wang, Gordan Lauc and Wei Wang

Improved CD4 T cell profile in HIV-infected subjects on maraviroc-containing therapy is associated with better responsiveness to HBV vaccination

Maraviroc-containing combined antiretroviral therapy (MVC-cART) improved the response to the hepatitis B virus (HBV) vaccine in HIV-infected subjects younger than 50 years old. We aimed here to explore the eff...

Authors: Inés Herrero-Fernández, Isaac Rosado-Sánchez, Miguel Genebat, Laura Tarancón-Díez, María Mar Rodríguez-Méndez, María Mar Pozo-Balado, Carmen Lozano, Ezequiel Ruiz-Mateos, Manuel Leal and Yolanda M. Pacheco

Cytokines induced killer cells produced in good manufacturing practices conditions: identification of the most advantageous and safest expansion method in terms of viability, cellular growth and identity

Cytokine-induced killer (CIK) cells are a very promising cell population raising growing interest in the field of cellular antitumor therapy. The aim of our study was to validate the most advantageous expansio...

Authors: Sara Castiglia, Aloe Adamini, Deborah Rustichelli, Laura Castello, Katia Mareschi, Giuseppe Pinnetta, Marco Leone, Alessandra Mandese, Ivana Ferrero, Giulia Mesiano and Franca Fagioli

Large-scale in silico identification of drugs exerting sex-specific effects in the heart

Major differences exist between men and women in both physiology and pathophysiology. Dissecting the underlying processes and contributing mechanisms of sex differences in health and disease represents a cruci...

Authors: Changting Cui, Chuanbo Huang, Kejia Liu, Guoheng Xu, Jichun Yang, Yong Zhou, Yingmei Feng, Georgios Kararigas, Bin Geng and Qinghua Cui

Clinical significance of hyaluronan levels and its pro-osteogenic effect on mesenchymal stromal cells in myelodysplastic syndromes

Hyaluronan (HA), a major component of the extracellular matrix, has been proven to play a crucial role in tumor progression. However, it remains unknown whether HA exerts any effects in myelodysplastic syndrom...

Authors: Cheng-Ming Fei, Juan Guo, You-Shan Zhao, Si-Da Zhao, Qing-Qing Zhen, Lei Shi, Xiao Li and Chun-Kang Chang

NPM1 alternative transcripts are upregulated in acute myeloid and lymphoblastic leukemia and their expression level affects patient outcome

Expression of the NPM1 gene, encoding nucleophosmin, is upregulated in cancers. Although more than ten NPM1 transcripts are known, the reports were usually limited to one predominant transcript. In leukemia, the

Authors: Luiza Handschuh, Pawel Wojciechowski, Maciej Kazmierczak, Malgorzata Marcinkowska-Swojak, Magdalena Luczak, Krzysztof Lewandowski, Mieczyslaw Komarnicki, Jacek Blazewicz, Marek Figlerowicz and Piotr Kozlowski

RIP3 dependent NLRP3 inflammasome activation is implicated in acute lung injury in mice

NLRP3 inflammasome is involved in the inflammatory responses during acute lung injury (ALI). RIP3 triggered NLRP3 inflammasome activation independent of necroptosis induction has recently been documented. In t...

Authors: Jingxian Chen, Shuang Wang, Rong Fu, Mianjing Zhou, Tengyue Zhang, Wenxu Pan, Niansheng Yang and Yuefang Huang

The expression signature of very long non-coding RNA in myalgic encephalomyelitis/chronic fatigue syndrome

Myalgic encephalomyelitis/chronic fatigue syndrome (ME/CFS) is a chronic debilitating disease with huge social-economic impact. It has been suggested that immune dysregulation, nitrooxidative stress, and metab...

Authors: Chin-An Yang, Sandra Bauer, Yu-Chen Ho, Franziska Sotzny, Jan-Gowth Chang and Carmen Scheibenbogen

Blood pressure components and incident cardiovascular disease and mortality events among Iranian adults with chronic kidney disease during over a decade long follow-up: a prospective cohort study

To explore the association between systolic and diastolic blood pressure (SBP and DBP respectively) and pulse pressure (PP) with cardiovascular disease (CVD) and mortality events among Iranian patients with pr...

Authors: Ashkan Hashemi, Sormeh Nourbakhsh, Samaneh Asgari, Mohammadhassan Mirbolouk, Fereidoun Azizi and Farzad Hadaegh

YKL-40 is highly expressed in the epicardial adipose tissue of patients with atrial fibrillation and associated with atrial fibrosis

YKL-40 (CHI3L1) is a novel biomarker for inflammation, tissue remodeling, and fibrosis, as well as cardiovascular diseases. We investigated the association between YKL-40 expression in epicardial adipose tissue (...

Authors: Qing Wang, Hua Shen, Jie Min, Yang Gao, Kai Liu, Wang Xi, Jie Yang, Liang Yin, Jibin Xu, Jian Xiao and Zhinong Wang

Urinary exosome miR-146a is a potential marker of albuminuria in essential hypertension

There is increasing interest in using extracellular vesicle-derived microRNAs (miRNAs) as biomarkers in renal dysfunction and injury. Preliminary evidence indicates that miRNAs regulate the progression of glom...

Authors: Javier Perez-Hernandez, Dolores Olivares, Maria J. Forner, Ana Ortega, Elena Solaz, Fernando Martinez, Felipe J. Chaves, Josep Redon and Raquel Cortes

Enhanced effect of checkpoint inhibitors when given after or together with IMM-101: significant responses in four advanced melanoma patients with no additional major toxicity

The use of checkpoint inhibitors (ipilimumab, pembrolizumab, nivolumab) has revolutionised the treatment of metastatic melanoma. However still more than the half the patients do not respond to single-agent imm...

Authors: Angus G. Dalgleish, Satvinder Mudan and Alberto Fusi

Protocol for serum exosomal miRNAs analysis in prostate cancer patients treated with radiotherapy

Circulating exosomes from prostate cancer (PCa) patients undergoing radiotherapy are attractive candidate biomarkers for monitoring treatment response. Multiple workflows for isolation and content characteriza...

Authors: Bijaya Malla, Daniel M. Aebersold and Alan Dal Pra

Lactobacillus salivarius reverse antibiotic-induced lung defense impairment in a ventilator model

Widespread use of antibiotics in the intensive care unit is a potential cause of the emergence of hospital-acquired pneumonia. This study determined whether Lactobacillus salivarius feeding could reverse antibiot...

Authors: Tzyy-Bin Tsay, Ming-Chieh Yang, Wan-Hsuan Chang, Pei-Hsuan Chen and Lee-Wei Chen

Cord blood leptin and insulin levels in association with mitochondrial DNA content

The developmental origins of health and disease theory states that a disturbance in the early life environment can contribute to disease risk in later life. Leptin and insulin are anorectic hormones involved i...

Authors: Annette Vriens, Michelle Plusquin, Willy Baeyens, Liesbeth Bruckers, Elly Den Hond, Ilse Loots, Vera Nelen, Greet Schoeters, Bram G. Janssen and Tim S. Nawrot

HBV infection increases the risk of macular degeneration: the roles of HBx-mediated sensitization of retinal pigment epithelial cells to UV and blue light irradiation

Hepatitis B virus (HBV) infection is strongly associated with hepatocellular carcinoma due to the main pathogenic X protein of HBV (HBx). Whether HBV infection and the HBx protein could result in macular degen...

Authors: Ruey-Hwang Chou, Chang-Yin Lee, Lee-Won Chong, Kuang-Hsi Chang, Cheng-Li Lin, Ke-Sin Yan, Chingfu Tsou and Yi-Chao Hsu

Comparing atmospheric and hypoxic cultured mesenchymal stem cell transcriptome: implication for stem cell therapies targeting intervertebral discs

Mesenchymal stem cells (MSCs) represent an attractive avenue for cellular therapies targeting degenerative diseases. MSC in vitro expansion is required in order to obtain therapeutic numbers during the manufac...

Authors: C. Elabd, T. E. Ichim, K. Miller, A. Anneling, V. Grinstein, V. Vargas and F. J. Silva

A circRNA–miRNA–mRNA network identification for exploring underlying pathogenesis and therapy strategy of hepatocellular carcinoma

Circular RNAs (circRNAs) have received increasing attention in human tumor research. However, there are still a large number of unknown circRNAs that need to be deciphered. The aim of this study is to unearth ...

Authors: Dan-dan Xiong, Yi-wu Dang, Peng Lin, Dong-yue Wen, Rong-quan He, Dian-zhong Luo, Zhen-bo Feng and Gang Chen

Can IDO activity predict primary resistance to anti-PD-1 treatment in NSCLC?

Immune checkpoint inhibitors have revolutionized the treatment paradigm of highly lethal malignancies like advanced non-small cell lung cancer (NSCLC), demonstrating long-term tumour control and extended patie...

Authors: Andrea Botticelli, Bruna Cerbelli, Luana Lionetto, Ilaria Zizzari, Massimiliano Salati, Annalinda Pisano, Mazzuca Federica, Maurizio Simmaco, Marianna Nuti and Paolo Marchetti

Improving knowledge on the activation of bone marrow fibroblasts in MGUS and MM disease through the automatic extraction of genes via a nonnegative matrix factorization approach on gene expression profiles

Multiple myeloma (MM) is a cancer of terminally differentiated plasma that is part of a spectrum of blood diseases. The role of the micro-environment is crucial for MM clonal evolution.

Authors: Angelina Boccarelli, Flavia Esposito, Mauro Coluccia, Maria Antonia Frassanito, Angelo Vacca and Nicoletta Del Buono

The role of miR-29c/B7-H3 axis in children with allergic asthma

MicroRNAs play roles in the pathogenesis of bronchial asthma. However, the mechanism of miR-29c in allergic asthma remains unclear. This study is to elucidate the regulation of Th cell differentiation by miR-2...

Authors: Xinxing Zhang, Xin Zhao, Huiming Sun, Yongdong Yan, Li Huang, Wenjin Gu, Wujun Jiang, Yuqing Wang, Canhong Zhu, Wei Ji, Chuangli Hao and Zhengrong Chen

Regulatory network of circRNA–miRNA–mRNA contributes to the histological classification and disease progression in gastric cancer

Little has been known about the role of non-coding RNA regulatory network in the patterns of growth and invasiveness of gastric cancer (GC) development.

Authors: Jia Cheng, Huiqin Zhuo, Mao Xu, Linpei Wang, Hao Xu, Jigui Peng, Jingjing Hou, Lingyun Lin and Jianchun Cai

MRI of atherosclerosis and fatty liver disease in cholesterol fed rabbits

The globally rising obesity epidemic is associated with a broad spectrum of diseases including atherosclerosis and non-alcoholic fatty liver (NAFL) disease. In the past, research focused on the vasculature or ...

Authors: Erik Taylor, Nasi Huang, Jacob Bodde, Andrew Ellison, Ronald Killiany, Markus Michael Bachschmid and James Hamilton

Increased citrullination and expression of peptidylarginine deiminases independently of P. gingivalis and A. actinomycetemcomitans in gingival tissue of patients with periodontitis

A relationship between rheumatoid arthritis (RA) and periodontitis has been suggested from findings that individuals with RA are prone to have advanced periodontitis and vice versa. In search of possible commo...

Authors: Marianne Engström, Kaja Eriksson, Linkiat Lee, Monika Hermansson, Anders Johansson, Anthony P. Nicholas, Natalija Gerasimcik, Karin Lundberg, Lars Klareskog, Anca Irinel Catrina and Tülay Yucel-Lindberg

Intra-articular injection of two different doses of autologous bone marrow mesenchymal stem cells versus hyaluronic acid in the treatment of knee osteoarthritis: long-term follow up of a multicenter randomized controlled clinical trial (phase I/II)

Mesenchymal stromal cells (MSCs) are a promising option to treat knee osteoarthritis (OA). Their safety and usefulness have been reported in several short-term clinical trials but less information is available...

Authors: José María Lamo-Espinosa, Gonzalo Mora, Juan F. Blanco, Froilán Granero-Moltó, Jorge María Núñez-Córdoba, Silvia López-Elío, Enrique Andreu, Fermín Sánchez-Guijo, José Dámaso Aquerreta, José María Bondía, Andrés Valentí-Azcárate, María del Consuelo del Cañizo, Eva María Villarón, Juan Ramón Valentí-Nin and Felipe Prósper

Fibroblasts as a practical alternative to mesenchymal stem cells

Mesenchymal stem cell (MSC) therapy offers great potential for treatment of disease through the multifunctional and responsive ability of these cells. In numerous contexts, MSC have been shown to reduce inflam...

Authors: Thomas E. Ichim, Pete O’Heeron and Santosh Kesari

The new era of precision population health: insights for the All of Us Research Program and beyond

Although precision medicine has made advances in individualized patient treatments, there needs to be continued attention on tailored population health and prevention strategies (often termed “precision popula...

Authors: Courtney R. Lyles, Mitchell R. Lunn, Juno Obedin-Maliver and Kirsten Bibbins-Domingo

Comprehensive identification of immune-associated biomarkers based on network and mRNA expression patterns in membranous glomerulonephritis

Membranous glomerulonephritis (MGN) is the most common cause of nephrotic syndrome in adult patients. Despite extensive evidences suggested that many immune-related genes could serve as effective biomarkers in...

Authors: Chengwei Zhang, Lei Leng, Xiaoming Zhang, Yao Zhao and Zhaozheng Li

Numerical simulation of patient-specific endovascular stenting and coiling for intracranial aneurysm surgical planning

In this study, we develop reliable and practical virtual coiling and stenting methods for intracranial aneurysm surgical planning. Since the purpose of deploying coils and stents is to provide device geometrie...

Authors: Xiaochang Leng, Yang Wang, Jing Xu, Yeqing Jiang, Xiaolong Zhang and Jianping Xiang