Existence of a potential neurogenic system in the adult human brain
© Nogueira et al.; licensee BioMed Central Ltd. 2014
Received: 25 December 2013
Accepted: 13 March 2014
Published: 22 March 2014
Prevailingly, adult mammalian neurogenesis is thought to occur in discrete, separate locations known as neurogenic niches that are best characterized in the subgranular zone (SGZ) of the dentate gyrus and in the subventricular zone (SVZ). The existence of adult human neurogenic niches is controversial.
The existence of neurogenic niches was investigated with neurogenesis marker immunostaining in histologically normal human brains obtained from autopsies. Twenty-eight adult temporal lobes, specimens from limbic structures and the hypothalamus of one newborn and one adult were examined.
The neural stem cell marker nestin stained circumventricular organ cells and the immature neuronal marker doublecortin (DCX) stained hypothalamic and limbic structures adjacent to circumventricular organs; both markers stained a continuous structure running from the hypothalamus to the hippocampus. The cell proliferation marker Ki-67 was detected predominately in structures that form the septo-hypothalamic continuum. Nestin-expressing cells were located in the fimbria-fornix at the insertion of the choroid plexus; ependymal cells in this structure expressed the putative neural stem cell marker CD133. From the choroidal fissure in the temporal lobe, a nestin-positive cell layer spread throughout the SVZ and subpial zone. In the subpial zone, a branch of this layer reached the hippocampal sulcus and ended in the SGZ (principally in the newborn) and in the subiculum (principally in the adults). Another branch of the nestin-positive cell layer in the subpial zone returned to the optic chiasm. DCX staining was detected in the periventricular and middle hypothalamus and more densely from the mammillary body to the subiculum through the fimbria-fornix, thus running through the principal neuronal pathway from the hippocampus to the hypothalamus. The column of the fornix forms part of this pathway and appears to coincide with the zone previously identified as the human rostral migratory stream. Partial co-labeling with DCX and the neuronal marker βIII-tubulin was also observed.
Collectively, these findings suggest the existence of an adult human neurogenic system that rises from the circumventricular organs and follows, at minimum, the circuitry of the hypothalamus and limbic system.
The study of adult neurogenesis began a long time after the description of the neuron by Ramon y Cajal . This delay is partly attributed to the conclusions of another study published by Ramon y Cajal exactly one century ago, in which he stated: “In adult centers the nerve paths are something fixed, ended, immutable” and “Everything may die, nothing may be regenerated” . Those conclusions were labeled the “central dogma of neurobiology” . However, critics of Ramon y Cajal underestimate his postulations, which posed the following challenge: “It is for the science of the future to change, if possible, this harsh decree” [2, 3].
In fact, this challenge was not met until approximately half a century later. In 1962, Altman  reported the first evidence of neurogenesis in adult mammals. However, the studies conducted by Altman initially had little impact, principally because of two factors: the technical limitations at the time and the lack of perspective regarding the possible applications of the knowledge acquired. These types of studies were conducted in the 1980s and became increasingly common in the subsequent years due to the development of new techniques in molecular and cellular biology and an increased knowledge of the therapeutic potential of stem cells. Consequently, adult neurogenesis was described in birds , rodents  and humans .
In adult mammals, neurogenesis involves the proliferation and differentiation of neural stem cells (NSCs). By definition, NSCs display the ability of self-renewal and the potential to generate neurons, astrocytes and oligodendrocytes, cell types of the central nervous system. Studies principally conducted in rodents described two regions in which NSCs could be found—the so-called neurogenic niches—the subventricular zone (SVZ) and the subgranular zone (SGZ) of the dentate gyrus .
The description of a neurogenic niche is more controversial in humans than in rodents. Not all studies have demonstrated the existence of NSCs in the hippocampus of normal adult humans . The same applies to the rostral migratory stream (RMS), through which NSCs migrate toward the olfactory bulb and differentiate following their proliferation in the SVZ; the existence of this stream in humans has been debated in the literature [10–13]. Evidence to the contrary arose from cultures of neurospheres (clusters of NSCs and central nervous system cell types of clonal origin ). Neurospheres were developed from samples collected from areas with controversial neurogenic potential, such as the white matter, the isocortex and the amygdala .
The refinement of dissection and immunohistochemistry techniques may contribute to resolving the controversy related to the location of neurogenic niches in humans. Few protocols have been developed for this purpose [16–18] and certain technical details must be considered in practice, including the circumstances of death, the age of the deceased, the history of disease, the cause of death and the duration of the agonal state; all of these factors represent potential obstacles to obtaining reliable results. The time elapsed between death and tissue fixation presents a similar obstacle. In our study, this latter variable was significantly correlated with the likelihood of detecting NSCs in the SVZ.
We investigated the human temporal lobe because it contains acknowledged and supposed locations of neurogenic niches. The SGZ and the SVZ contained cells expressing the NSC marker nestin. However, these cells occupied part of a continuous layer connected by the subpial zone (SPZ) of the medial temporal lobe and centered at the fimbria. The fimbria is in contact with both the intraventricular and extraventricular cerebrospinal fluid (CSF) and with the choroid plexus , where the blood–brain barrier is absent ; these features may contribute to the modulation of neurogenesis . Moreover, the fimbria forms part of the circuitries related to more elementary brain functions such as autonomic control and emotion . In this context, we discovered that neurogenesis markers were expressed between the hippocampus and the hypothalamus in one newborn and in one adult human. The staining of the neurogenesis markers was more pronounced in the hypothalamus and a dense staining of the immature neuronal marker doublecortin (DCX) was observed throughout the fimbria-fornix system. The expression of the neurogenesis markers in the hypothalamus-hippocampus axis suggests a persistence of neurogenic potential orchestrated in a large area of the brain.
Materials and methods
This study was performed with the approval of the Research Ethics Committee of the Clinical Hospital of the University of São Paulo Faculty of Medicine and the São Paulo Municipal Department of Death Certification, both located in the city of São Paulo, Brazil.
Criteria for sample inclusion
The criteria for the inclusion of brains were the same as those used in similar studies involving autopsies. We dissected the adult brains of individuals who died at the age of 16 years or older and who did not die from a malignant brain neoplasm or any primary brain disease (detected clinically or during autopsy). The time elapsed between death and tissue fixation was less than 24 h.
Dissection of the brains
Immunohistochemistry and histology
The samples were fixed in a 4% formaldehyde solution (pH 7.2) at room temperature for 24 h, dehydrated and subsequently embedded in paraffin. The blocks were cut into 5-μm sections and mounted on silane-coated slides.
1:200 (F), 1:5000 (C)
Neuroblast, immature neuron
Immature and mature neuron
The secondary antibodies used for fluorescence immunohistochemistry included Alexa Fluor® 488 goat anti-rabbit (1:200, catalog no. A11008, Invitrogen Corporation, Carlsbad, CA, USA) and Alexa Fluor® 555 goat anti-mouse (1:750, catalog no. A21422, Invitrogen). Reagents for nuclear staining included Hoechst 33342 nucleic acid stain (1:1000, catalog no. H1399, Invitrogen) and DAPI (1:1000 w/v, catalog no. 46190, Thermo Scientific, Rockford, IL, USA). The antifading agent Vectashield® (catalog no. H1000, Vector Laboratories, Inc., Burlingame, CA, USA) was used. Additional file 1: Table S1 details the protocol developed for single and double staining by fluorescence immunohistochemistry. In each experiment, the negative control consisted of a section of the same specimen for which the immunofluorescence protocol was performed, but without the primary antibodies. In all figures, green and red colors depict the reactions of primary antibodies produced in the rabbit and mouse, respectively. Nuclear staining with Hoechst or DAPI appears in blue.
Ready-to-use kits were used according to the manufacturers’ recommendations for chromogenic immunohistochemistry (revealed in brown by 3,3′-diaminobenzidine (DAB)). For nestin staining by chromogenic immunohistochemistry, a labeled streptavidin-biotin kit was used (Dako LSAB + System-HRP, catalog no. K0690, Dako). For vimentin staining, the avidin-biotin technique was used (Super ABC kit®, 1:200, code EP-ABCu, Novocastra Laboratories, Newcastle upon Tyne, UK). For proliferating cell nuclear antigen (PCNA), Ki-67, CD133 and βIII-tubulin staining, we used the Reveal® biotin-free detection system (catalog no. SPB-125H, Spring Bio, Pleasanton, CA, USA) and the DAB-Plus reagent set (catalog no. 00-2020, Invitrogen), according to the manufacturers’ recommendations. Chromogenic immunohistochemistry experiments included an antigen retrieval phase during which the slides were maintained for 3 min 30 sec in citric acid buffer (pH 6.0) in a domestic pressure cooker. Hematoxylin was used to counterstain nuclei. Hematoxylin-eosin staining followed a standard protocol.
Equipment and software used for image acquisition and editing with respective figures
Nikon Optiphot-2 microscope* and CoolSNAP-Pro CF color digital camera#
2B, 2D, 3C–F, 5, S1
Nikon Eclipse E800 microscope and DMX-1200C digital camera*
Zeiss LSM 510 UV META microscope§
LSM 510 META§
2F, 10, 16
Zeiss LSM 780 – NLO microscope§
Pannoramic MIDI slide scanner ¬
Pannoramic viewer 1.15.2 RTM ¬
3B, 4, 6-9, 12-15, 17-32, 34D, S2, S3
Descriptive statistics and statistical inference (Mann–Whitney U test) displayed in Additional file 2: Table S2 and Additional file 3: Table S3 were performed using the Statistica Trial Version software (Stat Soft, Tulsa, OK, USA). The results were regarded as significant when p < 0.05 for a unilateral test. Regarding pixel intensity analysis, the ImageJ 1.47 t software (National Institutes of Health, Bethesda, MD, USA) and Excel 2013 (Microsoft, Redmond, WA, USA) were used to perform the descriptive statistics and Student’s t- test, respectively.
Data related to the autopsies and detection of NPCL in the SVZ
Cause of death
Time elapsed between death and autopsy
NPCL in the SVZ
14 h 50 min
11 h 50 min
18 h 20 min
5 h 10 min
16 h 40 min
22 h 35 min
14 h 5 min
Carcinoma of the mouth/pharynx
10 h 45 min
23 h 5 min
14 h 50 min
Data related to the newborn brain and the adult brain (hypothalamus and limbic structures)
Cause of death
Time elapsed between death and autopsy
Prematurity (28 weeks and 2 days of gestation at birth)
Systemic arterial hypertension
Congestive heart failure (ejection fraction = 34%)
The results were influenced by technical conditions not related to the immunohistochemistry experiments. For example, nestin staining in the SVZ of the collateral eminence was detected in six of 14 brains (Table 3). The time elapsed between death and tissue fixation was significantly related to the detection of nestin staining in the SVZ (p = 0.004) (Additional file 2: Table S2); however, the elapsed time did not significantly predict the detection of nestin staining in the SVZ until 16 h 40 min (p = 0.057) (Additional file 3: Table S3).
From the fimbria, the NPCL ran medially toward the SPZ (Figures 3, 4, 5 and 7) and followed an anterior and inferior trajectory. Thus, the subpial component of the NPCL [19, 25–28] was related to the following components of the hippocampal formation: the hippocampus proper (layers CA1-CA4 in the head of the hippocampus and CA3 and CA4 in the body of the hippocampus), the fimbria, the dentate gyrus and the subiculum. In the amygdala, the subpial NPCL was related to the centromedian group of nuclei . From the fimbria, the NPCL also ran laterally and occupied the SVZ of the temporal lobe (Figures 2, 5 and 6), which was related to the following structures [19, 25–28]: the amygdala (the basolateral group of nuclei) , the hippocampus proper (primarily into the stratum oriens) and the collateral eminence. In the SVZ, the NPCL varied in thickness such that it was thicker near blood vessels (Figures 2 and 6).
In summary, a panoramic view of the results showed that the adult hypothalamus expressed markers of neurogenesis in a pattern that expands from the fimbria-fornix to the hippocampus and is similar in the adult and in the newborn brain.
Immunohistochemical analysis of human brain samples represents an alternative approach for neurogenesis experiments in adult humans . Based on our results, we recommend that the time between death and tissue fixation does not exceed 16 h when devising an immunohistochemistry protocol to study neurogenesis markers in adult humans; thus, the development of a rapid autopsy program [33–35] is desirable. In the indirect immunofluorescence protocol, we noticed that better nestin staining was achieved when the primary antibody solution was maintained on the slides for 48 h at 4°C (Additional file 1: Table S1)  instead of overnight at room temperature (data not shown); this finding should be taken into consideration when troubleshooting poor staining of human brain samples.
A caveat of our results is that the staining may not identify a neurogenic system. Indeed, the streptavidin-biotin method revealed biotin staining in negative controls in major bundles such as the fimbria-fornix. To avoid misinterpretation of immunostaining in these locations, methods that do not use biotin should be considered. Moreover, the decrease in autofluorescence varied by location; in particular, autofluorescence in the hypothalamus diminished but was not eliminated in neurons containing lipofuscin, in the leptomeninges and in fibers possibly expressing monoamines . Because the brain is relatively heterogeneous, the comparison of images of the same structure in adjacent sections (one containing a marker staining and the other serving as a negative control) minimized the risk of a technical artifact appearing to represent a specific staining pattern.
Other factors to be considered in the interpretation of the results are the premortem status of the patient and injury-induced neurogenesis. The staining of neurogenesis markers observed in this study may underestimate the distribution of markers that occurs in the normal adult brain in vivo, as certain patients had conditions (e.g., diabetes and alcoholism) that were degenerative and not associated with injury-induced neurogenesis . In particular, the adult brain that was most intensely examined was obtained after an agonal period that was insufficiently long to cause injury-induced neurogenesis in the brain cytoarchitecture. Moreover, this brain displayed corpora amylacea (Figure 23 and Additional file 6: Figure S3)  in a layer from the medial preoptic area to the anterior boundaries of the septal area and in the SVZ. The presence of corpora amylacea as observed in this study represents a normal finding of ageing . However, curiously, these concretions were located in zones containing putative NSCs. It is possible that the high frequency of corpora amylacea found in neuropsychiatric conditions such as Alzheimer’s disease is related to a diminished number of NSCs and therefore represents a marker for neurogenic system failure [39, 40].
A final caveat is that the lack of specific markers for NSCs and neurogenesis does not allow concluding that our results prove the existence of an adult human constitutive neurogenic system. Indeed, nestin is not a specific marker of the NSC , as it can be detected in ependymal  and endothelial cells  in the adult mammalian brain. Nonetheless, NPCs that display the morphology of astrocytes or immature cells in the SVZ (type B and type C cells) or SGZ (type I and type II cells) are presumed to be NSCs .
Similarly, the reason for the DCX staining shown here is unclear. DCX has been observed in neural cells that span a wide spectrum of phases of differentiation ranging from neuroblasts  or oligodendroglial progenitor cells  to mature neural cells . Between these extremes, DCX has been shown to be a marker of neural plasticity . In our study, DCX staining may indicate neurogenesis. DCX-positive cells were found in contact with NSCs and structures participating in the neurogenesis process—namely the ependyma [48–51] and the circumventricular organs [20, 52]. In spite of the controversies surrounding the topic, there is strong evidence that DCX is a marker for neurogenesis . Moreover, the pattern of DCX staining observed in our study was similar to that of other neurogenesis markers such as the polysialylated-neural cell adhesion molecule (PSA-NCAM) (see Figures 3, 4, and 5 in ref. ). The analysis of the pattern of distribution of neurogenesis markers in the brain cytoarchitecture contributed to the interpretation of our findings.
Bearing the caveats in mind, one possible explanation for the results is that the adult human brain harbors a constitutive neurogenic system. The hypothalamus  in particular has features that corroborate this explanation. The septo-hypothalamic continuum zone [29, 30] contains most of the circumventricular organs [20, 52], and because of its lack of a blood brain barrier, it is well positioned to sense systemic factors known to influence adult neurogenesis. In addition, these circumventricular organs are neurogenic niches that could provide feedback to systemic factors .
Accordingly, the median eminence contains hormone-secreting neurons, is adjacent to the pituitary portal system and contains NPCs that may be tanycytes (radial glia-like cells with progenitor cell features) (Figure 13) . The neurohypophysis is a circumventricular organ that was not evaluated in this study; however, neurogenesis markers were found in the zone of the paraventricular nucleus, which contains neurons that project to the neurohypophysis (Figures 12, 13 and 16) [56, 57]. In addition to being near the median eminence and the neurohypophysis, the NPCs in the zone of the organum vasculosum lamina terminalis are adjacent to dense leptomeninges and the ependyma of the third ventricle (Figures 12 and 13). The simultaneous localization in the SPZ and SVZ was a common feature of NPCs. Greater concentrations of Ki-67-positive cells were observed in this part of the hypothalamus, but studies examining the co-labeling of Ki-67 and nestin were not performed. The expression of Ki-67 and nestin in the same cell would not identify the fate of the cell, but the presence of DCX-positive cells in the hypothalamus raises the possibility of neuronal formation from NSCs during adulthood.
The staining pattern of neurogenesis markers follows the Papez circuit; the column of the fornix may thus represent the location of the zone previously referred to as the adult human RMS. This observation is in agreement with the finding that no neurogenesis markers were found in a coronal plane anterior to the third ventricle in humans (see Figure 4 in ref. ). In contrast, neurogenesis markers were found along a trajectory that resembles the path of the column of the fornix, which rises from the foramen of Monro (see Figure 1 in ref. ) and descends in a lateral direction. Although the anterior continuation of this trajectory  does not coincide with the fornix, it ends at the topography of the anterior perforate substance where we detected NPCs (Figures 3 and 13). Moreover, the pattern of staining in the location identified as the human RMS (see Figure 4G–J in ref. ) is similar to the pattern of staining in the column of the fornix shown here. Alternatively, the zone previously declared to be the human RMS may be related to the septal area, which is inferior to the rostrum of the corpus callosum and where DCX and Ki-67 are expressed . Although a straightforward comparison of our results with those of other studies cannot be conducted because the human brain was analyzed in different planes, our findings suggest that the location previously referred to as the human RMS is related to the fornix or the structures adjacent to the anterior boundary of the hypothalamus.
The distribution of neurogenesis markers between the hypothalamus and the hippocampus can be better appreciated along the choroidal fissure , although this distribution follows a similar pattern near the endorhinal sulcus and in the anterior commissure in the form of a ring-like structure. DCX expression was prominent in the fimbria-fornix, an area surrounded by NPCs at the junction between the fimbria-fornix and the choroid plexus (taenia fimbriae and taenia fornicis) ; this staining in the human may represent a baseline pattern that can be contrasted with the increased expression of DCX and nestin in the injured fimbria-fornix of rats . Moreover, the human choroid plexus ependymal cells are potential NSCs because they are embedded in a potentially neurogenic microenvironment surrounding the choroidal fissure  and express CD133 in the same way as embryonic NSCs  and glioblastoma stem cells ; in addition, rat choroid plexus ependymal cells display NSC features .
In the adult temporal lobe, we observed that the likelihood of detecting NPCs in a specific location tended to vary with the distance of the site from the pia mater and the ependyma, which are layers in contact with the CSF that converge at the choroid plexus. Accordingly, the NPCL runs from the taenia fimbriae across both the SVZ and the SPZ (Figure 30), becomes progressively less evident in the SPZ and is eventually minimized after leaving the allocortex (Figure 7). In the depth of the brain parenchyma, a discrete NPCL located principally in the medial temporal lobe was also observed to be in contact with blood vessels (data not shown), which is consistent with the findings on the role of the neurovascular unit in neurogenesis [63, 64]. In addition to its proximity to the choroidal fissure (relative to the volume of the entire brain), this NCPL is in contact with the CSF through the Virchow-Robin space of the neurovascular unit .
The existence of neurogenic potential in the medial temporal lobe SPZ is in agreement with other recent findings. First, in the embryonic counterparts of the SPZ (the marginal zone in mice  and the outer SVZ in humans ), neurogenesis can be triggered by the proliferation and differentiation of radial glial-like cells. These findings challenge the concept that fetal cortical neurogenesis occurs exclusively from the radial glial cells located in the ventricular zone and the SVZ. Second, NSCs have been detected in the SPZ of other mammals [68–75]. Third, GFAPδ, an isoform of GFAP, has an interesting staining pattern that is similar in terms of cell morphology and location (SVZ and SPZ) to that of nestin observed in this study . Fourth, in cases of mesial temporal sclerosis, NPCs have been found adjacent to the hippocampal fissure (i.e., in the molecular layers) , an extension of the hippocampal sulcus, where we found a remarkable number of NPCs. Finally, injury-induced neurogenesis has been described in the SVZ , SGZ  and SPZ  in a rat model of spreading depression.
The expression of DCX in the medial temporal lobe suggests that the DCX-positive fibers are related principally to the efferent pathway from the hippocampus to the hypothalamus . This pattern is unlikely to be related primarily to projections of the septal and hypothalamic nuclei to the hippocampal formation  because DCX was not detected in the fibers reaching granule cells of the dentate gyrus or in the pyramidal neurons of the hippocampus. Moreover, the major branch of the NPCL in the SPZ of the medial temporal lobe runs toward the subiculum (Figures 7 and 33). In addition, the granule cells of the dentate gyrus and their axons in the CA3 (mossy fibers) stained positive for the mature neuronal marker microtubule associated protein-2 (MAP-2) but not the immature neuronal markers DCX and βIII-tubulin (Figure 31). In contrast, DCX is expressed from the subiculum and depicts the principal efferent pathway to the hypothalamus (Figures 27, 28, 29 and 33).
Broadly, the DCX-positive fibers leaving the subiculum and heading to the hypothalamus follow a progressively more robust potential neurogenic microenvironment (i.e., the intraparenchymal subependymal layer, the fimbria-fornix and the septo-hypothalamic continuum) that could provide feedback for neurogenic modulation. Likewise, the highly vascularized choroid plexus  and the flow of the CSF  could guide neuroblast migration or axonal growth  across the connection from the hippocampus to the hypothalamus. Neurogenesis in the human subiculum could be related to the fact that the transition from a three-layer to a six-layer cortex occurs into this structure. Furthermore, the subiculum is the hippocampal formation structure that expanded the most from rodents to humans and contains two types of neurons with different firing patterns that could reflect different phases of maturation .
High rates of constitutive neurogenesis are not expected in humans  because NSCs are actively maintained in a quiescent state . Moreover, microenvironmental factors could modulate the cell cycle and the fate of novel neural cells that rise more frequently [84, 85] than previously thought. A subset of the DCX-positive cells may correspond to immature neurons in a “standby” mode [86, 87], as was similarly proposed for the neurons in the islands of Calleja [88, 89]. By a mechanism that remains to be clarified [81, 90–92], the neurogenic system may follow circadian  and circannual rhythms . Moreover, the neurogenic system may change in humans over their lifespan, as suggested by the shift in expression of neurogenesis markers, primarily ranging from the SPZ of the medial temporal lobe to the SGZ in newborns but shifting to primary expression from the SPZ to the subiculum in adults. Likewise, it has been shown that mammalian neurogenesis declines with ageing .
This study identified the core of the potential neurogenic system but not its boundaries. The image of an elusive neurogenic system that follows neuronal pathways  from its core across the hypothalamus, limbic system and reticular activating system represents a possible foundation for future studies of adult neurogenesis. Thus, the pineal gland may be part of the core of this potential neurogenic system. The pineal gland is a circumventricular organ [20, 52] with endocrine functions embedded in a region largely filled with choroid plexus and pia mater ; this gland is functionally and pathologically linked to the hypothalamus and the pituitary  and is adjacent to the limbic system. Advancing to the brain circuitry proper, the projection of the neurons of the supramammillary area to the dentate gyrus (via the molecular layer)  may cross the SPZ of the medial temporal lobe where the minor branch of the NPCL is located (Figures 4, 5, 7 and 30) and where βIII-tubulin is expressed (Figure 31). Importantly, the rat  and primate  counterparts of this location display features of neurogenic niches. Furthermore, the neurogenesis markers stained unspecific thalamic nuclei (Figure 25G–L) and the anterior thalamic nucleus (Figures 12, 14, 16 and 17). Likewise, DCX-positive fibers in the hypothalamus were found along a passage of the medial forebrain bundle  that reaches the limbic and reticular activating systems in the midbrain . At this point, a neurogenic system projecting from the reticular formation could be underpinned by the SVZ, where a serotoninergic plexus is located . Finally, the most remarkable link between this study and the literature is the dense area of DCX staining in the fornix, a central structure of the potential neurogenic system in humans and the location from which migrating neuroblasts emerge and migrate to the associative parietal cortex in primates .
Based on our results, we propose a hypothesis referred to as the “Big Braing” hypothesis whereby the intense fetal neurogenesis (the “brain’s Big Bang”) persists as a background process during adulthood. To exemplify this idea, the mechanisms by which the fetal fimbria-fornix expands from the lamina terminalis contribute to the temporal location of the adult human hippocampus [26, 101] and may remain partially active in the adult hypothalamus-hippocampus axis. Interestingly, this axis resembles the limbic lobe described in 1878 by Paul Broca as a “boundary” of the brain hemispheres  in a study without any apparent connections to the 1873 seminal manuscript of Camilo Golgi [1, 22], which described the methods that later underpinned the Ramon y Cajal conclusions mentioned in the Introduction. Processes related to high energetic demand that form a continuous spectrum that includes neuronal activity, metabolic stress and apoptosis could trigger neurogenesis. The emergence of neurogenesis from a core may be related to brain physiology as a whole via the propagation of interconnected brain functions consecutively involving homeostasis in the hypothalamus, emotion in the limbic system, level of consciousness in the reticular activating system and functions of specific systems (e.g., vision and cognition). If the “Big Braing” hypothesis is correct, the ultimate function of adult human neurogenesis is to extend the life cycle of the organism  beyond the life cycle of its cells (including neurons).
Adult human neurogenesis is controversial. Here, we show that NSC markers stain the circumventricular organs and the neurogenesis marker DCX stains the neural circuitry adjacent to the circumventricular organs. A panoramic view depicts a continuous structure between the hypothalamus and the hippocampus and suggests the existence of a potential neurogenic system in the adult human brain. The possible existence of a neurogenic flow through the brain is interesting and may be useful for the development of neuroregeneration therapies .
The authors would like to thank Dr. Regina Schultz, Professor Jarbas Arruda Bauer, Dr. Juliano Andreoli Miyake and the Molecular Biology of Cancer Group members of the Department of Cellular and Developmental Biology at the Institute of Biomedical Sciences of the University of São Paulo, São Paulo, Brazil, for their contributions to the study. The authors are also grateful to the Cell and Molecular Therapy Center staff of the University of São Paulo, São Paulo, Brazil, for their contributions to this study.
This study received financial support from the Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq, Brazilian National Council for Scientific and Technological Development; grants #552644/2005-6 and #401002/2013-6) and the Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP, São Paulo Research Foundation; grants #04/08908-2 and #2010/51634-1).
- Langmoen IA, Apuzzo ML: The brain on itself: nobel laureates and the history of fundamental nervous system function. Neurosurgery. 2007, 61: 891-907. 10.1227/01.neu.0000303185.49555.a9.PubMedGoogle Scholar
- Curtis MA, Eriksson PS, Faull RLM: Adult human neurogenesis: a response to cell loss and new circuitry requirements?. Adult Neurogenesis. Edited by: Gage FH, Kempermann G, Song H. 2008, Cold Spring Harbor: Cold Spring Harbor Laboratory Press, 619-643.Google Scholar
- Colucci-D’Amato L, Bonavita V, di Porzio U: The end of the central dogma of neurobiology: stem cells and neurogenesis in adult CNS. Neurol Sci. 2006, 27: 266-270. 10.1007/s10072-006-0682-z.PubMedGoogle Scholar
- Altman J: Are new neurons formed in the brains of adult mammals?. Science. 1962, 135: 1127-1128. 10.1126/science.135.3509.1127.PubMedGoogle Scholar
- Goldman SA, Nottebohm F: Neuronal production, migration and differentiation in a vocal control nucleus of the adult female canary brain. Proc Natl Acad Sci U S A. 1983, 80: 2390-2394. 10.1073/pnas.80.8.2390.PubMed CentralPubMedGoogle Scholar
- Reynolds BA, Weiss S: Generation of neurons and astrocytes from isolated cells of the adult mammalian central nervous system. Science. 1992, 255: 1707-1710. 10.1126/science.1553558.PubMedGoogle Scholar
- Kirschenbaum B, Nedergaard M, Preuss A, Barami K, Fraser RA, Goldman SA: In vitro neuronal production and differentiation by precursor cells derived from the adult human forebrain. Cereb Cortex. 1994, 4: 576-589. 10.1093/cercor/4.6.576.PubMedGoogle Scholar
- Farin A, Liu CY, Langmoen IA, Apuzzo MLJ: The biological restoration of central nervous system architecture and function: part 2—emergence of the realization of adult neurogenesis. Neurosurgery. 2009, 64: 581-601. 10.1227/01.NEU.0000343539.15177.D1.PubMedGoogle Scholar
- Blümcke I, Schewe JC, Normann S, Brüstle O, Schramm J, Elger CE, Wiestler OD: Increase of nestin-immunoreactive neural precursor cells in the dentate gyrus of pediatric patients with early-onset temporal lobe epilepsy. Hippocampus. 2001, 11: 311-321. 10.1002/hipo.1045.PubMedGoogle Scholar
- Curtis MA, Kam M, Nannmark U, Anderson MF, Axell MZ, Wikkelso C, Holtås S, van Roon-Mom WMC, Björk-Eriksson T, Nordborg C, Frisén J, Dragunow M, Faull RLM, Eriksson PS: Human neuroblasts migrate to the olfactory bulb via a lateral ventricular extension. Science. 2007, 315: 1243-1249. 10.1126/science.1136281.PubMedGoogle Scholar
- Sanai N, Nguyen T, Ihrie RA, Mirzadeh Z, Tsai HH, Wong M, Gupta N, Berger MS, Huang E, Garcia-Verdugo JM, Rowitch DH, Alvarez-Buylla A: Corridors of migrating neurons in the human brain and their decline during infancy. Nature. 2011, 478: 382-386. 10.1038/nature10487.PubMed CentralPubMedGoogle Scholar
- Sanai N, Tramontin AD, Quiñones-Hinojosa A, Barbaro NM, Gupta N, Kunwar S, Lawton MT, McDermott MW, Parsa AT, Manuel-García Verdugo J, Berger MS, Alvarez-Buylla A: Unique astrocyte ribbon in adult human brain contains neural stem cells but lacks chain migration. Nature. 2004, 427: 740-744. 10.1038/nature02301.PubMedGoogle Scholar
- Wang C, Liu F, Liu YY, Zhao CH, You Y, Wang L, Zhang J, Wei B, Ma T, Zhang Q, Zhang Y, Chen R, Song H, Yang Z: Identification and characterization of neuroblasts in the subventricular zone and rostral migratory stream of the adult human brain. Cell Res. 2011, 21: 1534-1550. 10.1038/cr.2011.83.PubMed CentralPubMedGoogle Scholar
- Moe MC, Varghese M, Danilov AI, Westerlund U, Ramm-Pettersen J, Brundin L, Svensson M, Berg-Johnsen J, Langmoen IA: Multipotent progenitor cells from the adult human brain: neurophysiological differentiation to mature neurons. Brain. 2005, 128: 2189-2199. 10.1093/brain/awh574.PubMedGoogle Scholar
- Arsenijevic Y, Villemure JG, Brunet JF, Bloch JJ, Déglon N, Kostic C, Zurn A, Aebischer P: Isolation of multipotent neural precursors residing in the cortex of the adult human brain. Exp Neurol. 2001, 170: 48-62. 10.1006/exnr.2001.7691.PubMedGoogle Scholar
- Bernier PJ, Vinet J, Cossette M, Parent A: Characterization of the subventricular zone of the adult human brain: evidence for the involvement of Bcl-2. Neurosci Res. 2000, 37: 67-78. 10.1016/S0168-0102(00)00102-4.PubMedGoogle Scholar
- Quiñones-Hinojosa A, Sanai N, Soriano-Navarro M, Gonzalez-Perez O, Mirzadeh Z, Gil-Perotin S, Romero-Rodriguez R, Berger MS, Garcia-Verdugo JM, Alvarez-Buylla A: Cellular composition and cytoarchitecture of the adult human subventricular zone: a niche of neural stem cells. J Comp Neurol. 2006, 494: 415-434. 10.1002/cne.20798.PubMedGoogle Scholar
- Waldvogel HJ, Curtis MA, Baer K, Rees MI, Faull RL: Immunohistochemical staining of post-mortem adult human brain sections. Nat Protoc. 2006, 1: 2719-2132.PubMedGoogle Scholar
- Wen HT, Rhoton AL, de Oliveira E, Cardoso AC, Tedeschi H, Baccanelli M, Marino R: Microsurgical anatomy of the temporal lobe: part 1: mesial temporal lobe anatomy and its vascular relationships as applied to amygdalohyppocampectomy. Neurosurgery. 1999, 45: 549-591. 10.1097/00006123-199909000-00028.PubMedGoogle Scholar
- Bennett L, Yang M, Enikolopov G, Iacovitti L: Circumventricular organs: a novel site of neural stem cells in the adult brain. Mol Cell Neurosci. 2009, 41: 337-347. 10.1016/j.mcn.2009.04.007.PubMed CentralPubMedGoogle Scholar
- Lehtinen MK, Zappaterra MW, Chen X, Yang YJ, Hill AD, Lun M, Maynard T, Gonzalez D, Kim S, Ye P, D’Ercole AJ, Wong ET, LaMantia AS, Walsh CA: The cerebrospinal fluid provides a proliferative niche for neural progenitor cells. Neuron. 2011, 69: 893-905. 10.1016/j.neuron.2011.01.023.PubMed CentralPubMedGoogle Scholar
- Swanson LW: Brain Architecture. 2012, New York: Oxford University PressGoogle Scholar
- Buchwalow IB, Böcker W: Immuno-histochemistry: Basics and Methods. 2010, Berlin: SpringerGoogle Scholar
- McKay BE, Molineux ML, Turner RW: Endogenous biotin in rat brain: implications for false-positive results with avidin-biotin and streptavidin-biotin techniques. Methods Mol Biol. 2008, 418: 111-128.PubMedGoogle Scholar
- Amaral D, Lavenex P: Hippocampal neuroanatomy. The Hippocampus Book. Edited by: Andersen P, Morris R, Amaral D, Bliss T, O’Keefe J. 2007, New York: Oxford University Press, 37-114.Google Scholar
- Duvernoy H: The Human Hippocampus. 2005, Berlin: SpringerGoogle Scholar
- Gloor P: The amygdaloid system. The Temporal and Limbic System. Edited by: Gloor P. 1997, New York: Oxford University Press, 591-721.Google Scholar
- Mai JK, Paxinos G, Voss T: Atlas of the Human Brain. 2008, Amsterdam: ElsevierGoogle Scholar
- Lautin A: Broca’s lobe. The Limbic Brain. Edited by: Lautin A. 2001, New York: Kluwer Academics / Plenum Publishers, 1-53.Google Scholar
- Nauta WJ: Hippocampal projections and related pathways to the midbrain in the cat. Brain. 1958, 81: 319-340. 10.1093/brain/81.3.319.PubMedGoogle Scholar
- Canteras NS, Swanson LW: Projections of the ventral subiculum to the amygdala, septum, and hypothalamus: a PHAL anterograde tract-tracing study in the rat. J Comp Neurol. 1992, 324: 180-194. 10.1002/cne.903240204.PubMedGoogle Scholar
- Eriksson PS, Perfilieva E, Björk-Eriksson T, Alborn AM, Nordborg C, Peterson DA, Gage FH: Neurogenesis in the adult human hippocampus. Nat Med. 1998, 4: 1313-1317. 10.1038/3305.PubMedGoogle Scholar
- Ghorpade A, Bruch L, Persidsky Y, Chin B, Brown WHC, Borgmann K, Persidsky R, Wu L, Holter S, Cotter R, Faraci J, Heilman D, Meyer V, Potter JF, Swindells S, Gendelman HE: Development of a rapid autopsy program for studies of brain immunity. J Neuroimmunol. 2005, 163: 134-144.Google Scholar
- Kretzschmar H: Brain banking: opportunities, challenges and meaning for the future. Nat Rev Neurosci. 2009, 10: 70-77. 10.1038/nrn2535.PubMedGoogle Scholar
- Swaab DF: Introduction. The Human Hypothalamus: Basic and Clinical Aspects. Part I: Nuclei of the Human Hypothalamus. Edited by: Swaab DF. 2003, Amsterdam: Elsevier, 3-38.Google Scholar
- Korzhevskii DE, Gilyarov A: Optimization of a method for the immunocytochemical detection of nestin in paraffin sections of the rat brain. Neurosci Behav Physiol. 2008, 38: 135-137. 10.1007/s11055-008-0019-x.PubMedGoogle Scholar
- Dahlström A, Fuxe K: Localization of monoamines in the lower brain stem. Experientia. 1964, 20: 398-399. 10.1007/BF02147990.PubMedGoogle Scholar
- Kang JO, Kim SK, Hong SE, Lee TH, Kim CJ: Low dose radiation overcomes diabetes-induced suppression of hippocampal neuronal cell proliferation in rats. J Korean Med Sci. 2006, 21: 500-505. 10.3346/jkms.2006.21.3.500.PubMed CentralPubMedGoogle Scholar
- Vinters HV, Kleinschmidt-DeMasters BK: General pathology of the central nervous system. Greenfield’s neuropathology. Edited by: Love S, Louis DN, Ellison DW. 2008, London: Hodder Arnold, 1-62.Google Scholar
- Kosik KS: Study neuron networks to tackle Alzheimer’s. Nature. 2013, 503: 31-32. 10.1038/503031a.PubMedGoogle Scholar
- Wiese C, Rolletschek A, Kania G, Blyszczuk P, Tarasov KV, Tarasova Y, Wersto RP, Boheler KR, Wobus AM: Nestin expression—a property of multi-lineage progenitor cells?. Cell Mol Life Sci. 2004, 61: 2510-2522. 10.1007/s00018-004-4144-6.PubMedGoogle Scholar
- Gu H, Wang S, Messam CA, Yao Z: Distribution of nestin immunoreactivity in the normal adult human forebrain. Brain Res. 2002, 943: 174-180. 10.1016/S0006-8993(02)02615-X.PubMedGoogle Scholar
- Palmer TD, Willhoite AR, Gage FH: Vascular niche for adult hippocampal neurogenesis. J Comp Neurol. 2000, 425: 479-494. 10.1002/1096-9861(20001002)425:4<479::AID-CNE2>3.0.CO;2-3.PubMedGoogle Scholar
- Couillard-Despres S, Winner B, Schaubeck S, Aigner R, Vroemen M, Weidner N, Bogdahn U, Winkler J, Kuhn HG, Aigner L: Doublecortin expression levels in adult brain reflect neurogenesis. Eur J Neurosci. 2005, 21: 1-14. 10.1111/j.1460-9568.2004.03813.x.PubMedGoogle Scholar
- Guo F, Maeda Y, Ma J, Xu J, Horiuchi M, Miers L, Vaccarino F, Pleasure D: Pyramidal neurons are generated from oligodendroglial progenitor cells in adult piriform cortex. J Neurosci. 2010, 30: 12036-12049. 10.1523/JNEUROSCI.1360-10.2010.PubMed CentralPubMedGoogle Scholar
- Verwer RW, Sluiter AA, Balesar RA, Baayen JC, Noske DP, Dirven CM, Wouda J, van Dam AM, Lucassen PJ, Swaab DF: Mature astrocytes in the adult human neocortex express the early neuronal marker doublecortin. Brain. 2007, 130: 3321-3335. 10.1093/brain/awm264.PubMedGoogle Scholar
- Knoth R, Singec I, Ditter M, Pantazis G, Capetian P, Meyer RP, Horvat V, Volk B, Kempermann G: Murine features of neurogenesis in the human hippocampus across the lifespan from 0 to 100 years. PLoS One. 2010, 5: e8809-10.1371/journal.pone.0008809.PubMed CentralPubMedGoogle Scholar
- Kuo CT, Mirzadeh Z, Soriano-Navarro M, Rasin M, Wang D, Shen J, Sestan N, Garcia-Verdugo J, Alvarez-Buylla A, Jan LY, Jan YN: Postnatal deletion of Numb/Numblike reveals repair and remodeling capacity in the subventricular neurogenic niche. Cell. 2006, 127: 1253-1264. 10.1016/j.cell.2006.10.041.PubMed CentralPubMedGoogle Scholar
- Sawamoto K, Wichterle H, Gonzalez-Perez O, Cholfin JA, Yamada M, Spassky N, Murcia NS, Garcia-Verdugo JM, Marin O, Rubenstein JL, Tessier-Lavigne M, Okano H, Alvarez-Buylla A: New neurons follow the flow of cerebrospinal fluid in the adult brain. Science. 2006, 311: 629-632. 10.1126/science.1119133.PubMedGoogle Scholar
- Carlén M, Meletis K, Göritz C, Darsalia V, Evergren E, Tanigaki K, Amendola M, Barnabé-Heider F, Yeung MS, Naldini L, Honjo T, Kokaia Z, Shupliakov O, Cassidy RM, Lindvall O, Frisén J: Forebrain ependymal cells are Notch-dependent and generate neuroblasts and astrocytes after stroke. Nat Neurosci. 2009, 12: 259-267. 10.1038/nn.2268.PubMedGoogle Scholar
- Chojnacki AK, Mak GK, Weiss S: Identity crisis for adult periventricular neural stem cells: subventricular zone astrocytes, ependymal cells or both?. Nat Rev Neurosci. 2009, 10: 153-163.PubMedGoogle Scholar
- McKinley MJ, Clarke IJ, Oldfield BJ: Circumventricular organs. The Human Nervous System. Edited by: Mai JK, Paxinos G. 2012, Amsterdam: Elsevier, 594-617.Google Scholar
- Chouaf-Lakhdar L, Fèvre-Montange M, Brisson C, Strazielle N, Gamrani H, Didier-Bazès M: Proliferative activity and nestin expression in periventricular cells of the adult rat brain. Neuroreport. 2003, 14: 633-636. 10.1097/00001756-200303240-00022.PubMedGoogle Scholar
- Gould E: Structural plasticity. The Hippocampus Book. Edited by: Andersen P, Morris R, Amaral D, Bliss T, O’Keefe J. 2007, New York: Oxford University Press, 321-342.Google Scholar
- Doetsch F, García-Verdugo JM, Alvarez-Buylla A: Cellular composition and three-dimensional organization of the subventricular germinal zone in the adult mammalian brain. J Neurosci. 1997, 17: 5046-5061.PubMedGoogle Scholar
- Swaab DF: Supraoptic and paraventricular nucleus (SON, PVN). The Human Hypothalamus: Basic and Clinical Aspects. Part I: Nuclei of the Human Hypothalamus. Edited by: Swaab DF. 2003, Amsterdam: Elsevier, 163-238.Google Scholar
- Marin JH: The hypothalamus and regulation of bodily functions. Neuroanatomy: Text and Atlas. Edited by: Martin JH. 2012, New York: McGraw-Hill, 355-384. 4Google Scholar
- Zhang X, Jin G, Li W, Zou L, Shi J, Qin J, Tian M, Li H: Ectopic neurogenesis in the forebrain cholinergic system-related areas of a rat dementia model. Stem Cells Dev. 2011, 20: 1627-1638. 10.1089/scd.2010.0285.PubMedGoogle Scholar
- Popescu BO, Gherghiceanu M, Kostin S, Ceafalan L, Popescu LM: Telocytes in meninges and choroid plexus. Neurosci Lett. 2012, 516: 265-269. 10.1016/j.neulet.2012.04.006.PubMedGoogle Scholar
- Uchida N, Buck DW, He D, Reitsma MJ, Masek M, Phan TV, Tsukamoto AS, Gage FH, Weisman IL: Direct isolation of human central nervous system stem cells. Proc Natl Acad Sci U S A. 2000, 97: 14720-14725. 10.1073/pnas.97.26.14720.PubMed CentralPubMedGoogle Scholar
- Singh SK, Hawkins C, Clarke ID, Squire JA, Bayani J, Hide T, Henkelman RM, Cusimano MD, Dirks PB: Identification of human brain tumour initiating cells. Nature. 2004, 432: 396-401. 10.1038/nature03128.PubMedGoogle Scholar
- Itokazu Y, Kitada M, Dezawa M, Mizoguchi A, Matsumoto N, Shimizu A, Ide C: Choroid plexus ependymal cells host neural progenitor cells in the rat. Glia. 2006, 53: 32-42. 10.1002/glia.20255.PubMedGoogle Scholar
- Snapyan M, Lemasson M, Brill MS, Blais M, Massouh M, Ninkovic J, Gravel C, Berthod F, Götz M, Barker PA, Parent A, Saghatelyan A: Vasculature guides migrating neuronal precursors in the adult mammalian forebrain via brain-derived neurotrophic factor signaling. J Neurosci. 2009, 29: 4172-4188. 10.1523/JNEUROSCI.4956-08.2009.PubMedGoogle Scholar
- Alliot F, Rutin J, Leenen PJ, Pessac B: Pericytes and periendothelial cells of brain parenchyma vessels co-express aminopeptidase N, aminopeptidase A, and nestin. J Neurosci Res. 1999, 58: 367-378. 10.1002/(SICI)1097-4547(19991101)58:3<367::AID-JNR2>3.0.CO;2-T.PubMedGoogle Scholar
- Iadecola C, Nedergaard M: Glial regulation of the brain microvasculature. Nat Neurosci. 2007, 10: 1369-1376. 10.1038/nn2003.PubMedGoogle Scholar
- Costa MR, Kessaris N, Richardson WD, Götz M, Hedin-Pereira C: The marginal zone/layer I as a novel niche for neurogenesis and gliogenesis in developing brain cortex. J Neurosci. 2007, 27: 11376-11388. 10.1523/JNEUROSCI.2418-07.2007.PubMedGoogle Scholar
- Hansen DV, Lui JH, Parker PR, Kriegstein AR: Neurogenic radial glia in the outer subventricular zone of human neocortex. Nature. 2010, 464: 554-561. 10.1038/nature08845.PubMedGoogle Scholar
- Bifari F, Decimo I, Chiamulera C, Bersan E, Malpeli G, Johansson J, Lisi V, Bonetti B, Fumagalli G, Pizzolo G, Krampera M: Novel stem/progenitor cells with neuronal differentiation potential reside in the leptomeningeal niche. J Cell Mol Med. 2009, 13: 3195-3208. 10.1111/j.1582-4934.2009.00706.x.PubMed CentralPubMedGoogle Scholar
- Ohira K, Furuta T, Hioki H, Nakamura KC, Kuramoto E, Tanaka Y, Funatsu N, Shimizu K, Oishi T, Hayashi M, Miyakawa T, Kaneko T, Nakamura S: Ischemia-induced neurogenesis of neocortical layer 1 progenitor cells. Nat Neurosci. 2010, 13: 173-179. 10.1038/nn.2473.PubMedGoogle Scholar
- Decimo I, Bifari F, Rodriguez FJ, Malpeli G, Dolci S, Lavarini V, Pretto S, Vasquez S, Sciancalepore M, Montalbano A, Berton V, Krampera M, Fumagalli G: Nestin-and doublecortin-positive cells reside in adult spinal cord meninges and participate in injury-induced parenchymal reaction. Stem Cells. 2011, 29: 2062-2076. 10.1002/stem.766.PubMed CentralPubMedGoogle Scholar
- Petricevic J, Forempoher G, Ostojic L, Mardesic-Brakus S, Andjelinovic S, Vukojevic K, Saraga-Babic M: Expression of nestin, mesothelin and epithelial membrane antigen (EMA) in developing and adult human meninges and meningiomas. Acta Histochem. 2011, 113: 703-711. 10.1016/j.acthis.2010.09.005.PubMedGoogle Scholar
- Decimo I, Bifari F, Krampera M, Fumagalli G: Neural stem cell niches in health and diseases. Curr Pharm Des. 2012, 18: 1755-1783. 10.2174/138161212799859611.PubMed CentralPubMedGoogle Scholar
- Nakagomi T, Molnár Z, Nakano-Doi A, Taguchi A, Saino O, Kubo S, Clausen M, Yoshikawa H, Nakagomi N, Matsuyama T: Ischemia-induced neural stem/progenitor cells in the pia mater following cortical infarction. Stem Cells Dev. 2011, 20: 2037-2051. 10.1089/scd.2011.0279.PubMedGoogle Scholar
- Petit A, Sanders AD, Kennedy TE, Tetzlaff W, Glattfelder KJ, Dalley RA, Puchalski RB, Jones AR, Roskams AJ: Adult spinal cord radial glia display a unique progenitor phenotype. PLoS One. 2011, 6: e24538-10.1371/journal.pone.0024538.PubMed CentralPubMedGoogle Scholar
- Ponti G, Crociara P, Armentano M, Bonfanti L: Adult neurogenesis without germinal layers: the “atypical” cerebellum of rabbits. Arch Ital Biol. 2010, 148: 147-158.PubMedGoogle Scholar
- Roelofs RF, Fischer DF, Houtman SH, Sluijs JA, Van Haren W, Van Leeuwen FW, Hol EM: Adult human subventricular, subgranular, and subpial zones contain astrocytes with a specialized intermediate filament cytoskeleton. Glia. 2005, 52: 289-300. 10.1002/glia.20243.PubMedGoogle Scholar
- Crespel A, Rigau V, Coubes P, Rousset MC, de Bock F, Okano H, Baldy-Moulinier M, Bockaert J, Lerner-Natoli M: Increased number of neural progenitors in human temporal lobe epilepsy. Neurobiol Dis. 2005, 19: 436-450. 10.1016/j.nbd.2005.01.020.PubMedGoogle Scholar
- Yanamoto H, Miyamoto S, Tohnai N, Nagata I, Xue JH: Induced spreading depression activates persistent neurogenesis in the subventricular zone, generating cells with markers for divided and early committed neurons in the caudate putamen and cortex. Stroke. 2005, 36: 1544-1550. 10.1161/01.STR.0000169903.09253.c7.PubMedGoogle Scholar
- Urbach A, Redecker C, Witte OW: Induction of neurogenesis in the adult dentate gyrus by cortical spreading depression. Stroke. 2008, 39: 3064-3072. 10.1161/STROKEAHA.108.518076.PubMedGoogle Scholar
- Xue JH, Yanamoto H, Nakajo Y, Tohnai N, Nakano Y: Induced spreading depression evokes cell division of astrocytes in the subpial zone, generating neural precursor-like cells and new immature neurons in the adult brain cortex. Stroke. 2009, 40: e606-e613. 10.1161/STROKEAHA.109.560334.PubMedGoogle Scholar
- Lu P, Wang Y, Graham L, McHale K, Gao M, Wu D, Brock J, Blesch A, Rosenzweig ES, Havton LA, Zheng B, Conner JM, Marsala M, Tuszynski MH: Long-distance growth and connectivity of neural stem cells after severe spinal cord injury. Cell. 2012, 150: 1264-1273. 10.1016/j.cell.2012.08.020.PubMed CentralPubMedGoogle Scholar
- Kohler SJ, Williams NI, Stanton GB, Cameron JL, Greenough WT: Maturation time of new granule cells in the dentate gyrus of adult macaque monkeys exceeds six months. Proc Natl Acad Sci U S A. 2011, 108: 10326-10331. 10.1073/pnas.1017099108.PubMed CentralPubMedGoogle Scholar
- Mira H, Andreu Z, Suh H, Lie DC, Jessberger S, Consiglio A, San Emeterio J, Hortigüela R, Marqués-Torrejón MA, Nakashima K, Colak D, Götz M, Fariñas I, Gage FH: Signaling through BMPR-IA regulates quiescence and long-term activity of neural stem cells in the adult hippocampus. Cell Stem Cell. 2010, 7: 78-89. 10.1016/j.stem.2010.04.016.PubMedGoogle Scholar
- Altman J: Autoradiographic investigation of cell proliferation in the brains of rats and cats. Anat Rec. 1963, 145: 573-591. 10.1002/ar.1091450409.PubMedGoogle Scholar
- Cifuentes M, Pérez-Martín M, Grondona JM, López-Ávalos MD, Inagaki N, Granados-Durán P, Rivera P, Fernández-Llebrez P: A comparative analysis of intraperitoneal versus intracerebroventricular administration of bromodeoxyuridine for the study of cell proliferation in the adult rat brain. J Neurosci Methods. 2011, 201: 307-314. 10.1016/j.jneumeth.2011.08.006.PubMedGoogle Scholar
- Marichal N, García G, Radmilovich M, Trujillo-Cenóz O, Russo RE: Enigmatic central canal contacting cells: immature neurons in “standby mode”?. J Neurosci. 2009, 29: 10010-10024. 10.1523/JNEUROSCI.6183-08.2009.PubMed CentralPubMedGoogle Scholar
- Gomez-Climent MA, Guirado R, Varea E, Nacher J: “Arrested development”: immature, but not recently generated, neurons in the adult brain. Arch Ital Biol. 2010, 148: 159-172.PubMedGoogle Scholar
- Bernier PJ, Parent A: Bcl-2 protein as a marker of neuronal immaturity in postnatal primate brain. J Neurosci. 1998, 18: 2486-2497.PubMedGoogle Scholar
- Swaab DF: Islands of Calleja, insulae terminalis. The Human Hypothalamus: Basic and Clinical Aspects. Part I: Nuclei of the Human Hypothalamus. Edited by: Swaab DF. 2003, Amsterdam: Elsevier, 61-62.Google Scholar
- Agnati LF, Guidolin D, Guescini M, Genedani S, Fuxe K: Understanding wiring and volume transmission. Brain Res Rev. 2010, 64: 137-159. 10.1016/j.brainresrev.2010.03.003.PubMedGoogle Scholar
- Fung SJ, Joshi D, Allen KM, Sivagnanasundaram S, Rothmond DA, Saunders R, Noble PL, Webster MJ, Weickert CS: Developmental patterns of doublecortin expression and white matter neuron density in the postnatal primate prefrontal cortex and schizophrenia. PLoS One. 2011, 6: e25194-10.1371/journal.pone.0025194.PubMed CentralPubMedGoogle Scholar
- Li Y, Lu H, Cheng PL, Ge S, Xu H, Shi SH, Dan Y: Clonally related visual cortical neurons show similar stimulus feature selectivity. Nature. 2012, 486: 118-121.PubMed CentralPubMedGoogle Scholar
- Bellesi M, Pfister-Genskow M, Maret S, Keles S, Tononi G, Cirelli C: Effects of sleep and wake on oligodendrocytes and their precursors. J Neurosci. 2013, 33: 14288-14300. 10.1523/JNEUROSCI.5102-12.2013.PubMed CentralPubMedGoogle Scholar
- Hazlerigg DG, Lincoln GA: Hypothesis: cyclical histogenesis is the basis of circannual timing. J Biol Rhythms. 2011, 26: 471-485. 10.1177/0748730411420812.PubMedGoogle Scholar
- Bouab M, Paliouras GN, Aumont A, Forest-Bérard K, Fernandes KJ: Aging of the subventricular zone neural stem cell niche: evidence for quiescence-associated changes between early and mid-adulthood. Neuroscience. 2011, 173: 135-149.PubMedGoogle Scholar
- Wen HT, Rhoton AL, de Oliveira E: Transchoroidal approach to the third ventricle: an anatomic study of the choroidal fissure and its clinical application. Neurosurgery. 1998, 42: 1205-1217. 10.1097/00006123-199806000-00001.PubMedGoogle Scholar
- Ellison DW, Perry A, Rosenblum M, Asa S, Reid R, Louis DN: Tumours: non-neuroepithelial tumours and secondary effects. Greenfield’s Neuropathology. Edited by: Love S, Louis DN, Ellison DW. 2008, London: Hodder Arnold, 2002-2182.Google Scholar
- Mercier F, Arikawa-Hirasawa E: Heparan sulfate niche for cell proliferation in the adult brain. Neurosci Lett. 2012, 510: 67-72. 10.1016/j.neulet.2011.12.046.PubMedGoogle Scholar
- Jahanshahi A, Temel Y, Lim LW, Hoogland G, Steinbusch HW: Close communication between the subependymal serotonergic plexus and the neurogenic subventricular zone. J Chem Neuroanat. 2011, 42: 297-303. 10.1016/j.jchemneu.2011.09.001.PubMedGoogle Scholar
- Gould E, Reeves AJ, Graziano MS, Gross CG: Neurogenesis in the neocortex of adult primates. Science. 1999, 286: 548-552. 10.1126/science.286.5439.548.PubMedGoogle Scholar
- Lautin A: MacLeans’s limbic system. The limbic brain. Edited by: Lautin A. 2001, New York: Kluwer Academics / Plenum Publishers, 69-98.Google Scholar
- Zhang G, Li J, Purkayastha S, Tang Y, Zhang H, Yin Y, Li B, Liu G, Cai D: Hypothalamic programming of systemic ageing involving IKK-β, NF-κB and GnRH. Nature. 2013, 497: 211-216. 10.1038/nature12143.PubMed CentralPubMedGoogle Scholar
- Registro Brasileiro de Ensaios Clínicos.http://www.ensaiosclinicos.gov.br/rg/RBR-55t5v9/,
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