Figure 13

Nestin expression in the hypothalamus. Arrowheads in Figures (A) and (D) ((B) and (E) are the respective negative controls) show NPCs, which display long processes (arrow in (A)) in the SPZ antero-superiorly covering fibers of the optic chiasm (OC). Figures (C) and (F) show the counterpart location in a section stained with hematoxylin-eosin (HE). Note the dense leptomeninges (LM) in this region. In the arcuate nucleus zone (Figures (G–L)), arrows point to putative NSCs that express nestin and display long processes that occasionally bifurcate (Figures (G), (I), (J) and (L)). These cells are near βIII-tubulin-expressing neuronal cells (see arrows in (H), (I), (K) and (L)). Other NPCs are endothelial cells. The arrow in Figure (O) points to a cell with a long process into the arcuate nucleus as revealed by hematoxylin-eosin staining. References for the locations analyzed in this panel are indicated by the black boxes and arrows drawn in the whole section of the hypothalamus in Figure 12. Scale bars: (A), (B), (D), (E), (J–L), (N), (O) = 50 μm; (C), (F–I), (M) = 100 μm.