Figure 9

Nestin expression at medium magnification in the newborn hypothalamus-hippocampus axis. This panel displays magnified views of the rectangles of Figure 8 showing the double immunostaining for GFAP (red) and nestin (green). Overall, periventricular structures have abundant nestin staining. Nestin staining is also observed to encompass the granule cell layer (GCL) from the SGZ (arrowhead in (N)) to the adjacent SPZ (arrow in (N)). GFAP expression shows similar patterns in the anuclear gap of the SVZ (arrows in (G) and (J)) and in the glia limitans of the SPZ (arrow in (M)); (D) shows both the SVZ underneath the ependyma (E) and the SPZ underneath the pia mater (PM) in the zone of the third ventricle (III). The principal structures in each line are: (A–C), mammillothalamic tract (MT) and posterior hypothalamic nucleus (PH); (D–F), mammillary body (MB); (G–I), amygdala; (J–L), SVZ at the roof of the temporal horn of the lateral ventricle (LV); (M–O), head of the hippocampus; (P–R), magnified view of the GCL and adjacent SGZ and SPZ. C, comb system; CP, cerebral peduncle; H, hypothalamus; HS, hypothalamic sulcus; US, uncal sulcus. Arrows: in (G) and (H), staining in ependymal cells; in (J) and (K), staining of processes through the ependyma and the SVZ; in (M), (N) and (P), staining in the SPZ; in (R), NPCs between the SPZ and the SGZ. Arrowheads: NPCs in the amygdala (H), the SVZ (K) and the SGZ (N). Scale bars: (A–C) = 1,000 μm; (D–F, M–O) = 500 μm; (G–L) = 100 μm; (P–S) = 50 μm.