Table 1 Primary antibodies
From: Existence of a potential neurogenic system in the adult human brain
Antibody | Type | Manufacturer | Catalog no. | Dilution | Phenotype |
|---|---|---|---|---|---|
Nestin | Rb | Millipore* | AB5922 | 1:200 (F), 1:5000 (C) | NSC |
Nestin | Rb | Abcam** | ab93666 | 1:100 | NSC |
Nestin | Ms | Abcam | ab22035 | 1:100 | NSC |
CD133 | Rb | Abcam | ab66141 | 1:100 | NSC |
GFAP | Ms | Abcam | ab80842 | 1:1200 | NSC, astrocyte |
Vimentin | Ms | Dako*** | M725 | 1:500 | NSC, astrocyte |
PCNA | Ms | Dako | M879 | 1:1000 | Cell cycle |
Ki-67 | Ms | Dako | M7187 | 1:500 | Cell cycle |
Glut-1 | Ms | Abcam | ab40084 | 1:100 | Blood-CSF barrier |
DCX | Rb | Abcam | ab18723 | 1:250 | Neuroblast, immature neuron |
βIII-tubulin | Ms | Abcam | ab47967 | 1:100 | Immature and mature neuron |
MAP-2 | Rb | Abcam | ab75059 | 1:300 | Mature neuron |