Figure 8

Nestin expression at low magnification in the newborn hypothalamus-hippocampus axis. (A), hematoxylin-eosin (HE) staining of a whole section at the coronal plane encompassing the mammillary body (MB) and the head of the hippocampus (HH). (B) and (C) are magnified views of the left and right rectangles of (A), respectively. (B) shows the mammillothalamic tract (MT). (C) shows the boundaries of the subiculum (S) with CA1 (right arrow) and the presubiculum (PS) (left arrow). (D–F), double immunostaining for GFAP (red) and nestin (green), respectively, displayed in a whole section of the same specimen. Rectangles in (D–F) identify the areas analyzed in Figure 9 and the names in (E) correspond to the principal structure that expresses nestin in each rectangle. The panoramic view for GFAP immunostaining facilitates the identification of the components of the lentiform nucleus (the internal and external globus pallidus (iP and eP, respectively) and the putamen (P)). Nestin expression primarily occurs in the periventricular and medial zones of the hypothalamus, as identified by the arrows pointing to the MT and the MB in (E). A, amygdala; C, claustrum; EC, entorhinal cortex; ES, endorhinal sulcus; GCL, granule cell layer; H, hypothalamus; HS, hypothalamic sulcus; I, insula; IC, internal capsule; IEL, intraparenchymal ependymal layer (see text); III, third ventricle; LV, lateral ventricle; NC, temporal neocortex; OT, optic tract; PH, posterior hypothalamic nucleus; SC, subicular complex; SVZ, subventricular zone; T, thalamus; US, uncal sulcus. Scale bars: (A) = 10,000 μm; (B) = 500 μm; (C) = 1,000 μm; (D–F) = 5,000 μm.