Figure 25

Double staining of neurogenesis markers surrounding the body of the lateral ventricle. The locations analyzed in Figures (A–C), (D–F), (G–I) and (J–L) represent box numbers 4, 5, 6 and 7, respectively, in Figure 23. Nestin expression (arrows in (A), (C), (D) and (F)) occurs in locations between the ependymal cell layer (e) and βIII-tubulin-expressing structures (longitudinally cut fibers indicated by arrowheads in the corpus callosum (cc) in (B) and (C) and transversally cut fibers in the stria terminalis (st)). DCX stains fibers co-labeled with βIII-tubulin in the stria terminalis (arrowheads in (G–I)) and largely in the reticular nucleus (rn) (arrows in (G–L)), where both markers stain horizontally oriented fibers (J–L); certain vertically oriented fibers express DCX (arrowheads in (J–L)) but not βIII-tubulin. (f), fornix; (lv), lateral ventricle. Scale bars: (A–C), (J–M), (O) = 100 μm; (D–F), (N) = 50 μm; (G–I) = 20 μm.