Figure 10

Nestin expression at high magnification in the newborn hypothalamus-hippocampus axis. This panel shows GFAP (red), nestin (green) and nuclei (blue) in confocal plane images. This magnification reveals nestin expression surrounding the endorhinal sulcus (ES) zone (J–L), a location between the hypothalamus and the hippocampus. Note the dense nestin and the weak GFAP staining in the mammillothalamic tract (MT). (A–C), posterior hypothalamic nucleus; (D–F), MT zone; (G–I), mammillary body; (M–O), SVZ. Arrows point to cells co-labeled for GFAP and nestin. E, ependyma; III, third ventricle; PM, pia mater. Scale bars: 20 μm.