Figure 15

DCX and βIII-tubulin double staining in the hypothalamus studied by slide scanner. For reference, Figures (A–C), (D–F) and (G–I) correspond to magnified views of the upper, middle and lower boxes, respectively, as indicated by red arrows in Figure 12. Here, the arrows in the columns denote DCX, βIII-tubulin and Merge, referring to the structures stained for DCX (green) or βIII-tubulin (red), respectively. Additionally, the arrowheads indicate structures that stained for both DCX and βIII-tubulin. The DCX-positive cell in the paraterminal gyrus (arrows in (A) and (C)) shows granular staining near the nucleus, a pattern observed in certain DCX-positive cells in other regions. The cell bodies stained in the paraterminal gyrus and in the dorsomedial nucleus (DM nu) zone predominantly express DCX, whereas the fibers running through the ventromedial nucleus (VM nu) zone express DCX and βIII-tubulin in general. Figures (J–L) are images from the counterpart locations in the negative control. Scale bars: (A–C), (G–J), (L) = 50 μm; (D–F), (K) = 100 μm.