Figure 30

Staining of neurogenesis markers in the coronal section of the body of the hippocampus. The NPCL spreads from the taenia fimbriae (tf, arrow in (G)) to a layer formed by the pial surface (upper arrowhead in (G)) and the SPZ (spz in (G–I)) of the fimbriodentate sulcus (arrows in (I)) and to the SVZ of the hippocampus proper (lower arrowhead in (G)). These locations contain abundant GFAP staining (E), similar to the GFAP staining in the subependymal zone adjacent to the intraparenchymal ependymal cell layer (iel in (F)) (the inset in A is a magnified view of the box indicated by arrowhead in this same figure). (A), section of the entire body of the hippocampus and surrounding structures stained with hematoxylin-eosin (HE). (B), whole section of the same specimen stained with GFAP (red) and DAPI (blue). (D–F), magnified views of the respective boxes indicated in Figures (B) and (D). (C), negative control and boxes indicating the locations of Figures (J–L). (G–I), counterpart images for the analysis of nestin staining (red). In (A–C), arrows near the stria terminalis (st) and the fimbria (f) indicate the points of insertion of the choroid plexus (cp) to the parenchyma. Numbers 1-4 refer to the respective CA fields. (cn), tail of the caudate nucleus; (Cp), cerebral peduncle; (cs), collateral sulcus; (e), ependymal cell layer; (ec), entorhinal cortex; (gcl), granule cell layer of the dentate gyrus; (lgn), lateral geniculate nucleus; (lv), lateral ventricle; (pas), parasubiculum; (phg), parahippocampal gyrus (cytoarchitecture definition); (ps), presubiculum; (rn), red nucleus; (s), subiculum; (sas), subarachnoid space; (sn), substantia nigra; (th), thalamus; (wm), white matter of the parahippocampal gyrus (gross anatomy definition). Scale bars: (A), (B), (C) = 5,000 μm; (D) = 2,000 μm; (E) = 200 μm; (F–L), inset in (A) = 50 μm.