Figure 32

ΒIII-tubulin expression at the level of the head of the hippocampus. This panel shows the negative control of a panoramic view of a coronal section at the head of the hippocampus (A) counterstained with hematoxylin, also used for counterstaining in Figures (B–D). The boxes in (A) indicate the counterpart locations of figures (B–D), which are images from a section used to study βIII-tubulin expression (brown). βΙΙΙ-tubulin-positive fibers (arrowheads in (B)) are continuations of fibers connecting the head of the hippocampus and the amygdala (amy). At this level, the choroid plexus (cp) is not connected to the parenchyma; the connection between these structures occurs posteriorly from the inferior choroidal point. Moreover, βIII-tubulin staining depicts the efferent pathway of the hippocampal formation, which rises from cell bodies in the subiculum (arrowhead in (C)), runs through the subpial zone (spz) of the subiculum, curves at the presubiculum (D) and reaches the layer beneath the intraparenchymal ependymal layer (iel) and the subventricular zone of the lateral ventricle (lv) (arrows at right in (C)). (CA), cornu ammonis; (cs), collateral sulcus; (e), ependymal cell layer; (ec), entorhinal cortex; (phg), parahippocampal gyrus; (pm), pia mater; (sc), subicular complex; (u), uncus; (us), uncal sulcus. Scale bars: (A) = 5,000 μm; (B) = 100 μm; (C), (D) = 500 μm.