Obesity suppresses circulating level and function of endothelial progenitor cells and heart function
- Tzu-Hsien Tsai†1,
- Han-Tan Chai†1,
- Cheuk-Kwan Sun2,
- Chia-Hung Yen3,
- Steve Leu4,
- Yung-Lung Chen1,
- Sheng-Ying Chung1,
- Sheung-Fat Ko5,
- Hsueh-Wen Chang†6,
- Chiung-Jen Wu1 and
- Hon-Kan Yip†1, 7Email author
© Tsai et al.; licensee BioMed Central Ltd. 2012
Received: 25 January 2012
Accepted: 7 June 2012
Published: 2 July 2012
Background and aim
This study tested the hypothesis that obesity suppresses circulating number as well as the function of endothelial progenitor cells (EPCs) and left ventricular ejection fraction (LVEF).
High fat diet (45 Kcal% fat) was given to 8-week-old C57BL/6 J mice (n = 8) for 20 weeks to induce obesity (group 1). Another age-matched group (n = 8) were fed with control diet for 20 weeks as controls (group 2). The animals were sacrificed at the end of 20 weeks after obesity induction.
By the end of study period, the heart weight, body weight, abdominal fat weight, serum levels of total cholesterol and fasting blood sugar were remarkably higher in group 1 than in group 2 (all p<0.01). The circulating level of EPCs (C-kit/CD31, Sca-1/KDR, CXCR4/CD34) was significantly lower in group 1 than in group 2 (p<0.03) at 18 h after critical limb ischemia induction. The angiogenesis and migratory ability of bone marrow-derived EPCs was remarkably impaired in group 1 compared to that in group 2 (all p<0.01). The repair ability of aortic endothelium damage by lipopolysaccharide was notably attenuated in group 1 compared with that in group 2 (p<0.01). Collagen deposition (Sirius red staining) and fibrotic area (Masson's Trichrome staining) in LV myocardium were notably increased in group 1 compared with group 2 (p<0.001). LVEF was notably lower, whereas LV end-diastolic and end-systolic dimensions were remarkably higher in group 1 than in group 2 (all p<0.001).
Obesity diminished circulating EPC level, impaired the recovery of damaged endothelium, suppressed EPC angiogenesis ability and LVEF, and increased LV remodeling.
KeywordsObesity Aortic endothelium Endothelial progenitor cells Angiogenesis Left ventricular remodeling
Substantial evidence has shown that obesity is commonly associated with a broad range of cardiovascular diseases (CVD), including chronic inflammation, lipid metabolic disorder, accelerated atherosclerosis, increased risk for thrombosis, hypertension, hyperinsulinemia, insulin resistance, and type 2 diabetes mellitus [1–6]. Epidemiological study has further demonstrated that patients with obesity are twice as likely to die of ischemic heart disease compared to those without obesity [7, 8]. Indeed, previous studies have identified obesity as an important independent risk factor for cardiovascular events [9–12].
Vascular wall (VW) remodeling from endothelial dysfunction, smooth muscle migration and proliferation, medial-layer thickening, and decreased number of circulating EPCs and VW- resident EPCs in vasa vasorum/adventitia is essential in the development of all stages of atherosclerosis, from initiation, progression, and finally evolvement of complications [13–17]. An association between accumulated CVD risk factors and decreased circulating EPC number [18–21] has been extensively investigated. Additionally, EPCs have been well documented to play an essential role in repairing endothelial function and preventing atherosclerosis [22, 23].
Interestingly, while the association between the manifestation of CVD risk factors and decreased circulating number of EPCs [18–21] has been fully investigated, whether obesity itself is an independent factor affecting the number of circulating EPCs is still unclear. Moreover, the impact of obesity on the function of bone marrow-derived (BMD) EPCs has not been studied. Furthermore, of particular significance in translational research is whether obesity suppresses the ability of angiogenesis and vascular repair in response to ischemic stimulation and toxin-induced vascular endothelial damage, respectively.
Therefore, by using a high fat diet-induced murine obesity model, this study aimed at testing the hypotheses that obesity: 1) reduces the number of circulating EPCs, 2) impairs the angiogenic ability of BMDEPCs, 3) hinders the repair of damaged aortic endothelial cells, 4) suppresses left ventricular ejection fraction (LVEF), and 5) aggravates LV remodeling.
Materials and methods
All animal experimental procedures were approved by the Institute of Animal Care and Use Committee at our institute and performed in accordance with the Guide for the Care and Use of Laboratory Animals (NIH publication No. 85–23, National Academy Press, Washington, DC, USA, revised 1996).
Animal model of obesity
Eight-week-old male C57J/BL6 mice (n = 18), weighing 22–26 gm, (Charles River Technology, BioLASCO Taiwan Co., Ltd., Taiwan), were fed with high fat diet (45 Kcal% fat; Research Diets, Inc) to obtain the diet-induced obesity model for this study. According to the literature  and the instructions from the company (Research Diets, Inc), successful obesity induction in mice was defined as an increase in body weight more than 35% after feeding the animals with high fat diet (45 Kcal% fat) for 13–14 weeks. Therefore, obese mice 20–22 weeks old were utilized for the purpose of this study. By the end of 12 weeks, 18 of 24 mice were observed to fit the criteria of obesity. To determine whether cardiovascular disease develops after prolonged feeding with high fat diet, the 18 obese mice were continuously fed with high fat diet for two additional months (Group 1). For the purpose of the study, another group of aged-matched C57J/BL6 mice (n = 18) purchased from the same company was fed with a control diet for the same period to serve as normal controls (Group 2).
Animal model of critical limb ischemia for stimulating EPC mobilization into circulation
Mice in groups 1 and 2 were anesthetized by inhalation of 2.0% isoflurane. The mice were placed in a supine position on a warming pad at 37°C with the left hind limbs shaved. Under sterile conditions, the left femoral artery, small arterioles, and circumferential femoral artery were exposed and ligated over their proximal and distal portions before removal. To avoid the presence of collateral circulation, the branches were removed together. Blood was drawn for measuring EPCs using flow cytometry before being sacrificed at 18 h after CLI induction.
Functional assessment by echocardiography
Transthoracic echocardiography was performed in all animals by a cardiologist blinded to the design of the experiment using a sonographic machine for research purpose (VisualSonics, Vevo, 2100) at the beginning and end of the study with the mice under anesthesia in a supine position. M-mode tracings of left ventricle (LV) were obtained with the heart being imaged in a 2-dimensional mode in long-axis at the level between papillary muscle and mitral valve. Left ventricular internal dimensions [end-systolic diameter (ESD) and end-diastolic diameter (EDD)] were measured according to the American Society of Echocardiography leading-edge method using at least three consecutives cardiac cycles. LVEF was calculated as follows: .
Protocol and assessment of lipopolysaccharide-damaged aortic endothelial cells
This study was designed not only to test the number and function of EPCs, but it also assessed the effect of lipopolysaccharide (LPS) on the severity of aortic endothelial damage and vaso-relaxation impairment in obese and control mice. For this purpose, six mice from each group were randomly chosen to receive LPS treatment.
To elucidate the safety and optimal dosage of LPS for inducing aortic endothelial damage, three dosages of LPS (i.e. 1, 2, and 3 μM intra-peritoneal injection) were given to two mice in each group (i.e. obese and control group). While the highest dosage (3.0 μM) caused mortality, only the moderate dosage was found to cause a significant difference of EPC number in the aortic intimal layer between the two groups of survivors. The moderate dosage of LPS, therefore, was used in the current study.
Flow cytometric quantification of endothelial progenitor cells
For blood sampling at different time points (i.e. prior to CLI and at 18 h and on day 14 after induction of CLI), cardiac puncture instead of the venous route was adopted for blood sampling using a 30# needle. After treatment with red blood cell-lysing buffer, the cells remained were labeled with appropriate antibodies. Flow cytometric analysis for identification of cell surface markers was performed based on our recent reports . Briefly, the cells were immunostained for 30 minutes with PE-conjugated antibodies against CD31 (BioLegend), KDR (BD Pharmingen), CD34 (BD Pharmingen) and FITC-conjugated antibodies against Sca-1 (BD Pharmingen), c-Kit (BD Pharmingen), CXCR4 (BD Pharmingen). Isotype-identical antibodies (IgG) served as controls. Flow cytometric analyses were performed by utilizing a fluorescence-activated cell sorter (Beckman Coulter FC500 flow cytometer).
Bone marrow mononuclear cell culture, differentiation of endothelial cell phenotype, angiogenesis, and measurement of total tubular length
To evaluate the degree of angiogenesis, bone marrow was drawn from both obese and control animals. The protocol and procedure of cell culture and the determination of angiogenesis were described in our recent report . Briefly, MNCs were isolated by density-gradient centrifugation of Ficoll 400, followed by cultivation in differential endothelial cell culture medium (endothelial cell basal medium-2, Cambrex) with 10% fetal bovine serum (FBS), 50 U/mL penicillin, 50 μg/mL streptomycin and 2 mmol/L L-glutamine (Invitrogen) with vascular endothelial growth factor (VEGF) and basic fibroblast growth factor (10 ng/mL) plated on gelatin-coated tissue culture flasks and incubated at 37°C with 5% CO2 for 21 days. Culture medium was changed every 48 hours. By day 21, cells with spindle-shaped and cobblestone-like phenotype typical of endothelial cells were found attached on the plate.
The cells with endothelial cell phenotype were then plated in 96-well plates at 1.0 × 104 cells/well in 150 μL serum-free M199 culture medium mixed with 50 μL cold Matrigel (Chemicon international) for 24 hours using passage 2 EPCs incubated at 37°C in 5% CO2. Three random microscopic images (200 ×) were taken from each well for counting cluster, tube, and network formations with the mean values obtained. Both cumulative and mean tube lengths were calculated by Image-Pro Plus software (Media Cybernetics).
Transwell migratory assay
To investigate the transwell migratory ability of EPCs (n = 6 in each experiment), transwell membranes (5 μm; Costar, Germany) were coated on both sides with fibronectin (2.5 μg/mL; Roche, Mannheim, Germany) overnight at 4 °C. The experimental protocol was also based on that of our recent report . EPCs (1 × 105 cells/well) were resuspended in M199 medium (Gibco, Carlsbad, CA, USA) containing 0.5 FBS (Gibco, Carlsbad, CA, USA) and incubated in the upper chamber at 37°C in 5% CO2 and allowed to migrate for 18 hours toward the lower chamber which was filled with M199 containing 20% FBS. Cells remaining on the upper surface of the transwell membranes were mechanically removed and cells that had migrated to the lower surface were fixed with 4% formaldehyde. For cell quantification, nuclei of the migrated cells were stained with DAPI. Cells migrating into the lower chamber were counted over 5 random microscopic fields using a fluorescence microscope (Olympus, Tokyo, Japan) with the software Image-Pro Plus (Media Cybernetics, Bethesda, MD, USA).
Immunofluorescent (IF) staining
To determine the number of EPCs localized in the endothelial and adventitia (i.e. around the vasa vasorum) layer, IF staining was performed for the examinations of C-kit+, CD34+, Flk-1+ cells (n = 6 for each group) using respective primary antibodies as we previously reported . Irrelevant antibodies were used as controls in the present study.
Immunohistochemical (IHC) staining
To analyze the extent of collagen synthesis and deposition, three cardiac paraffin sections (6 μm) at 3 mm intervals were stained with picro-Sirius red (1% Sirius red in saturated picric acid solution) for one hour at room temperature using standard methods. The sections were then washed twice with 0.5% acetic acid. The water was physically removed from the slides by vigorous shaking. After dehydration in 100% ethanol thrice, the sections were cleaned with xylene and mounted in a resinous medium. High-power fields (×100) of each section were used to identify Sirius red-positive area on each section. Image-pro plus 6.1 software (Media Cybernetics, Inc., Bethesda, MD, USA) was used to calculate the total area of Sirius red-positive staining. The mean area of collagen deposition was obtained by summation of Sirius red-positive areas on each section divided by the total numbers of sections.
Histological study of fibrosis area in LV
Masson's Trichrome staining was used for studying fibrosis of LV myocardium. The integrated area (μm2) of fibrosis in LV myocardium in the tissue sections was calculated using Image Tool 3 (IT3) image analysis software (University of Texas, Health Science Center, San Antonio, UTHSCSA; Image Tool for Windows, Version 3.0, USA) as described previously . Three selected sections were quantified for each animal. Three randomly selected high-power fields (HPFs) (400 ×) were analyzed in each section. After determining the number of pixels in each fibrosis area per HPF, the numbers of pixels obtained from the three HPFs were summated. The procedure was repeated in two other sections for each animal. The mean pixel number per HPF for each animal was then determined by summating all pixel numbers and dividing by 9. The mean area of fibrosis per HPF was obtained using a conversion factor of 19.24 (1 μm2 represented 19.24 pixels).
Measurement of aortic contractility and nitric oxide release on LPS overload
At the end of the study, six mice in each group were treated by LPS. Forty-eight hours after LPS treatment, the mice were sacrificed and the aorta in each mouse was isolated. Aortic segments in each mouse were collected for identification of EPCs localized in aortic endothelial layer using IHC staining. Other aortic segments were cleaned and cut into slices of 2 mm in length for evaluating the contractile and relaxant response as previously reported  with some modifications. Briefly, the aortic rings were carefully mounted on an isometric force transducer (XDFT05, Singa, Taiwan) with a tension of 1.8 g and placed in an organ chamber filled with Krebs solution (NaCl, 99.01 mM; KCl, 4.69 mM; CaCl2, 1.87 mM; MgSO4, 1.20 mM; K2HPO4, 1.03 mM; glucose, 11.1 mM) maintained at pH 7.4 and bubbled with 95% O2 - 5% CO2. After an equilibration of 40 minutes, 1 μM of phenylephrine (PE) was added to the organ chamber for the assessment of contractile activity, and then 30 μM of acetylcholine (ACh) was added to assess the endothelial integrity. After washing and a re-equilibration for 30 minutes, a cumulative PE dose (from 1 nM to 1 μM) was added to the organ chamber to obtain a concentration-dependent contractile curve, and then sodium nitroprusside (30 μM) was added to the organ chamber to obtain a relaxant response. After washing and a re-equilibration for 20 minutes, 30 μM of ACh was added into the organ chamber, followed by 1 μM of PE to evaluate the endothelium-dependent vasorelaxant response. Then PE (1 μM)-induced vasocontractile response was assessed again in the presence of L-NAME (100 μM) pre-treatment for 30 minutes. All data were acquired and analyzed using the XctionView system (XctionView, Singa, Taiwan).
Protocol for assessment of aortic basal nitric oxide (NO) release on LPS overload
Vascular basal nitric oxide release was calculated as the percentage of difference between PE-induced vasocontractile response in the absence and presence of L-NAME according to our previous study .
Quantitative data are expressed as means ± SD. Statistical analysis was adequately performed by ANOVA followed by Tukey’s multiple comparison procedure. Statistical analysis was performed using SAS statistical software for Windows version 8.2 (SAS institute, Cary, NC). A probability value <0.05 was considered statistically significant.
Baseline characteristics, laboratory findings, and transthoracic echocardiography results
The baseline characteristics, laboratory findings and echocardiography results
Obesity (n = 8)
Control (n = 8)
Initial body weight (g)
25.7 ± 0.66
26.0 ± 0.72
Final body weight (g)
43.9 ± 1.55
34.4 ± 1.48
Heart weight (g)
0.19 ± 0.02
0.14 ± 0.01
Ratio of heart weight to tibial bone length
0.095 ± 0.002
0.070 ± 0.001
Abdominal fat weight (g)
4.09 ± 0.65
1.66 ± 0.41
Total cholesterol (mg/dl)
232.8 ± 7.53
136.3 ± 11.8
Initial fasting blood glucose level (mg/dl)†
103 ± 8.63
98.3 ± 9.0
Final fasting blood glucose level (mg/dl)†
307.5 ± 74.7
142.5 ± 18.3
Circulating level of endothelial progenitor cells
Baseline (0 h)‡
1.15 ± 0.21
1.06 ± 0.40
2.60 ± 0.49
3.29 ± 0.50
0.85 ± 0.27
0.66 ± 0.21
1.05 ± 0.24
1.60 ± 0.22
Baseline (0 h)‡
0.38 ± 0.17
0.43 ± 0.27
0.68 ± 0.31
1.13 ± 0.36
Inter-ventricular septum (mm)
0.82 ± 0.08
0.83 ± 0.09
Posterior wall (mm)
0.77 ± 0.11
0.76 ± 0.09
Left ventricular end-diastolic dimension (mm)
4.10 ± 0.10
3.93 ± 0.26
Left ventricular end-systolic dimension
2.71 ± 0.21
2.63 ± 0.23
Fractional shortening (%)
32.7 ± 1.98
34.4 ± 2.01
Left ventricular ejection fraction (%)
62.5 ± 2.43
64.0 ± 2.49
Echocardiograph (at end of study period)
Inter-ventricular septum (mm)
0.87 ± 0.06
0.86 ± 0.08
Posterior wall (mm)
0.79 ± 0.08
0.77 ± 0.03
Left ventricular end-diastolic dimension (mm)
4.46 ± 0.17b
4.18 ± 0.16a
Left ventricular end-systolic dimension
3.46 ± 0.48b
2.78 ± 0.13a
Fractional shortening (%)
22.8 ± 1.15b
33.4 ± 0.85a
Left ventricular ejection fraction (%)
46.0 ± 1.95b
62.7 ± 1.43a
The initial LVEF, fractional shortening (FS), LVEDd, LVESd and the thickness of interventricular septum and posterior wall did not differ between groups 1 and 2. Moreover, at the end of the study, the thickness of interventricular septum and posterior wall were similar between groups 1 and 2. However, at the end of study, LVEF and fractional shortening were significantly lower, whereas LVEDd and LVESd were remarkably higher in group 1 than in group 2.
Circulating level of EPC at baseline and 18 h after critical limb ischemia (CLI) procedure
Interestingly, at day 0 prior to the procedure, the circulating levels of EPCs (C-kit/CD31, Sca-1/KDR, CXCR4/CD34) were similar between group 1 and group 2. However, at 18 h after the CLI procedure, these biomarkers were notably lower in group 1 than in group 2 (Table 1). These findings implicate that EPC mobilization from bone marrow to circulation in response to ischemic stimuli was markedly impaired in obesity.
IHC and IF staining of aorta
Satining of left ventricular myocardium
Sirius red staining for collagen deposition and Masson's trichrome staining for fibrosis in left ventricular myocardium
Aortic vasorelaxation and NO release
Baseline KCl-induced vasoconstriction did not differ in obesity as compared with normal control regardless with and without LPS treatment (Figure 7-B). Baseline PE-induced vasoconstriction was notably reduced in group 1 compared to that in group 2. Besides, after LPS treatment, the responsibility of PE-induced vasoconstriction was weaker in group 1 than in group 2. By contrast, ACh-induced vasorelaxation exhibited an opposite manner compared to that of PE-induced vasoconstriction in baseline condition and after LPS treatment in both groups of animals (Figure 7-C & 7-D). The results indicate that the VW of obese mice was in a hyper-reactive state toward both vasodilatation and vasoconstrictive stimuli.
In vitro studies for measurement of total tubular length, transwell migratory assay, and proliferation assessment
This study, which investigated the impact of obesity on cardiovascular pathogenesis in a murine obesity model, yielded several striking implications. First, repair ability of EPC in response to LPS-induced damage of aortic endothelium was remarkably impaired in obese animals. Second, the circulating level of EPCs in the setting of CLI was significantly reduced in obese mice compared to that in normal mice. Third, LV remodeling was markedly increased, whereas LV function was notably suppressed in obese mice compared with the normal control animals. Fourth, hyper-reactive vasorelaxation and over compensatory NO production from aorta was observed in the obese animals. Finally, the number of EPCs and vasa vasorum in adventitia layer of epicardial arteries was significantly reduced in the setting of obesity.
In the present study, not only does the entire body weight increase, but also heart weight, ratio of heart weight to tibial length, abdominal fat weight, and total cholesterol level were substantially higher in obese animals than those in control animals. These findings suggest that the high-fat diet induced a successful obesity model in mice. One important finding is that feeding with high-fat diet also induced hyperglycemia.
Clinical observational studies have previously shown that the circulating number of EPCs was significantly decreased in manifestation of CVD risk factors and the presence of coronary artery disease (CAD) [18–21]. Interestingly, in the present study, histological analysis of the LV sections with α-SMA staining showed no evidence of atherosclerotic obstructive CAD in obese mice. Additionally, the baseline circulating levels of EPC were similar between obese and normal control mice. Our results, therefore, in addition to supporting the findings of previous studies [18–21], also provide useful information that the circulating number of EPCs may be normal in the setting of obesity without manifestation of atherosclerotic obstructive CAD.
The link between obesity and endothelial and vascular dysfunction has been extensively discussed recently [29, 30]. An essential finding in the current study is that, as compared with the normal controls, an increase in the circulating number of EPCs in response to ischemic stress was remarkably suppressed in obese mice. This finding indicates that the EPC mobilization ability from bone marrow to circulation in response to ischemic stimulation is curtailed in obesity. Moreover, aortic endothelial repair ability after LPS-induced endothelial damage was found to be notably reduced in obese animals. Furthermore, Matrigel assay demonstrated that the angiogenesis ability, an index of endothelial function, was substantially suppressed in the setting of obesity. Moreover, migratory assay identified that the EPC migratory ability was significantly hindered in obese animals compared to that in their normal counterparts. These findings highlight the fact that EPC/endothelial function (i.e., angiogenesis, EPC kinetics) rather than EPC number is reduced before the manifestation of obstructive CAD. In this way, our findings reinforce those of previous clinical observational studies [29, 30].
Although the role of obesity in impairing LV function and increasing LV remodeling have been keenly investigated in previously clinical observational studies, the study of heart function in animal models of obesity has seldom been reported [31–33]. The most important finding in this experimental study is that LVEF and FS were significantly lower in obese mice than those in normal controls. Of importance is that LV remodeling (i.e., increased LVEDd and LVESd) was remarkably increased in obese animals compared to that in their normal counterparts. Accordingly, the results of the present study support the findings from previous clinical observational studies [31–33]. Another principal finding in the current study using Masson's Trichrome staining showed that the fibrotic area was substantially increased in obese mice. Consistently, Sirius red staining revealed significantly heavier deposition of collagen fiber in LV myocardium of obese mice than that of normal controls. Furthermore, the numbers of vasa vasorum and EPCs over adventitia of epicardial vessels were significantly reduced in the former than in the latter animals. These findings may, at least in part, explain the notable impairment in LV function significant increase in LV remodeling in the obese mice even in the absence of atherosclerotic obstructive CAD (i.e. α-SMA results of patent epicardial vessels). Surprisingly, the underlying mechanisms involved in cardiac remodeling in obese patients without overt atherosclerosis-related CAD have not been investigated in previous clinical observational studies [31–33]. Our results, therefore, further extend the findings of those studies [31–33].
On the other hand, previous studies have emphasized that obesity results in an imbalance between endothelium-derived vasoactive factors favoring vasoconstriction and inflammatory activation. Abnormal regulation of these factors due to endothelial cell dysfunction is both the consequence and cause of vascular disease processes . Thus, obesity can be considered to cause accelerated "premature" vascular aging . Interestingly, the results of the present study demonstrated notably increased baseline NO release from the aorta in obese mice than that in the normal controls (Figure 7-A). Additionally, NO release after LPS-induced aortic endothelial damage was still significantly higher in the former than in the latter (Figure 7-A). Based on this finding, we propose that this phenomenon may be due to 1) a compensatory response to obesity-induced endothelial dysfunction at the early stage in a scenario comparable to hyper-insulinemic response in the setting of early hyperglycemic/diabetes mellitus situation; 2) it may also be due to a relatively higher intrinsic resistance of murine aortic endothelial cells against obesity-induced damage compared to that in the human beings. This second hypothesis could be further supported by the finding (Figure 7-B) that KCl-induced vasoconstriction (i.e. the integrity of calcium channel in smooth muscle cells) did not differ between obese and normal control mice. Our findings could further explain the notably lower PE-induced vasoconstriction (Figure 7-C), but apparently higher the ACh-induced vasorelaxation (Figure 7-D) in obese mice than those in normal controls. In this way, our findings are not completely identical to the those previously reported [34, 35].
This study has limitations. First, the exact underlying mechanisms involved in the impairment of heart function were not fully investigated in the current study. Second, the effects of therapeutic intervention and statin treatment on body weight, hypercholesterolemia, heart function, and circulating level of EPCs were not examined in the present study.
This study demonstrated that the circulating number and function of EPCs as well as heart function were markedly reduced, whereas LV remodeling was significantly enhanced in obesity even without overt atherosclerotic obstructive CAD.
This study was supported by a program grant from Chang Gung Memorial Hospital, Chang Gung University (Grant number: CMRPG 890641).
- Goodpaster BH, Krishnaswami S, Harris TB, Katsiaras A, Kritchevsky SB, Simonsick EM, Nevitt M, Holvoet P, Newman AB: Obesity, regional body fat distribution, and the metabolic syndrome in older men and women. Arch Intern Med. 2005, 165: 777-783.View ArticlePubMedGoogle Scholar
- Permana PA, Menge C, Reaven PD: Macrophage-secreted factors induce adipocyte inflammation and insulin resistance. Biochem Biophys Res Commun. 2006, 341: 507-514.View ArticlePubMedGoogle Scholar
- Tilg H, Moschen AR: Adipocytokines: mediators linking adipose tissue, inflammation and immunity. Nat Rev Immunol. 2006, 6: 772-783.View ArticlePubMedGoogle Scholar
- Chen H: Cellular inflammatory responses: novel insights for obesity and insulin resistance. Pharmacol Res. 2006, 53: 469-477.View ArticlePubMedGoogle Scholar
- Delarue J, Magnan C: Free fatty acids and insulin resistance. Curr Opin Clin Nutr Metab Care. 2007, 10: 142-148.View ArticlePubMedGoogle Scholar
- Cani PD, Amar J, Iglesias MA, Poggi M, Knauf C, Bastelica D, Neyrinck AM, Fava F, Tuohy KM, Chabo C, Waget A, Delmee E, Cousin B, Sulpice T, Chamontin B, Ferrieres J, Tanti JF, Gibson GR, Casteilla L, Delzenne NM, Alessi MC, Burcelin R: Metabolic endotoxemia initiates obesity and insulin resistance. Diabetes. 2007, 56: 1761-1772.View ArticlePubMedGoogle Scholar
- Rabkin SW, Mathewson FA, Hsu PH: Relation of body weight to development of ischemic heart disease in a cohort of young north american men after a 26 year observation period: The manitoba study. Am J Cardiol. 1977, 39: 452-458.View ArticlePubMedGoogle Scholar
- Hubert HB, Feinleib M, McNamara PM, Castelli WP: Obesity as an independent risk factor for cardiovascular disease: A 26-year follow-up of participants in the framingham heart study. Circulation. 1983, 67: 968-977.View ArticlePubMedGoogle Scholar
- Allison DB, Fontaine KR, Manson JE, Stevens J, VanItallie TB: Annual deaths attributable to obesity in the united states. JAMA. 1999, 282: 1530-1538.View ArticlePubMedGoogle Scholar
- Grundy SM: Obesity, metabolic syndrome, and coronary atherosclerosis. Circulation. 2002, 105: 2696-2698.View ArticlePubMedGoogle Scholar
- Eckel RH, Barouch WW, Ershow AG: Report of the national heart, lung, and blood institute-national institute of diabetes and digestive and kidney diseases working group on the pathophysiology of obesity-associated cardiovascular disease. Circulation. 2002, 105: 2923-2928.View ArticlePubMedGoogle Scholar
- Adams KF, Schatzkin A, Harris TB, Kipnis V, Mouw T, Ballard-Barbash R, Hollenbeck A, Leitzmann MF: Overweight, obesity, and mortality in a large prospective cohort of persons 50 to 71 years old. N Eng J Med. 2006, 355: 763-778.View ArticleGoogle Scholar
- van der Wal AC, Becker AE, van der Loos CM, Das PK: Site of intimal rupture or erosion of thrombosed coronary atherosclerotic plaques is characterized by an inflammatory process irrespective of the dominant plaque morphology. Circulation. 1994, 89: 36-44.View ArticlePubMedGoogle Scholar
- Ross R: Atherosclerosis: an inflammatory disease. N Engl J Med. 1999, 340: 115-126.View ArticlePubMedGoogle Scholar
- Yip HK, Hung WC, Yang CH, Chen YH, Cheng CI, Chen SM, Yeh KH: Serum concentrations of high-sensitivity c-reactive protein predict progressively obstructive lesions rather than late restenosis in patients with unstable angina undergoing coronary artery stenting. Circ J. 2005, 69: 1202-1207.View ArticlePubMedGoogle Scholar
- Orrico C, Pasquinelli G, Foroni L, Muscara D, Tazzari PL, Ricci F, Buzzi M, Baldi E, Muccini N, Gargiulo M, Stella A: Dysfunctional vasa vasorum in diabetic peripheral artery obstructive disease with critical lower limb ischaemia. Eur J Vasc Endovasc Surg. 2010, 40: 365-374.View ArticlePubMedGoogle Scholar
- Werner N, Kosiol S, Schiegl T, Ahlers P, Walenta K, Link A, Bohm M, Nickenig G: Circulating endothelial progenitor cells and cardiovascular outcomes. N Eng J Med. 2005, 353: 999-1007.View ArticleGoogle Scholar
- Lambiase PD, Edwards RJ, Anthopoulos P, Rahman S, Meng YG, Bucknall CA, Redwood SR, Pearson JD, Marber MS: Circulating humoral factors and endothelial progenitor cells in patients with differing coronary collateral support. Circulation. 2004, 109: 2986-2992.View ArticlePubMedGoogle Scholar
- Valgimigli M, Rigolin GM, Fucili A, Porta MD, Soukhomovskaia O, Malagutti P, Bugli AM, Bragotti LZ, Francolini G, Mauro E, Castoldi G, Ferrari R: CD34+ and endothelial progenitor cells in patients with various degrees of congestive heart failure. Circulation. 2004, 110: 1209-1212.View ArticlePubMedGoogle Scholar
- Chironi G, Walch L, Pernollet MG, Gariepy J, Levenson J, Rendu F, Simon A: Decreased number of circulating cd34 + kdr + cells in asymptomatic subjects with preclinical atherosclerosis. Atherosclerosis. 2007, 191: 115-120.View ArticlePubMedGoogle Scholar
- Asahara T, Masuda H, Takahashi T, Kalka C, Pastore C, Silver M, Kearne M, Magner M, Isner JM: Bone marrow origin of endothelial progenitor cells responsible for postnatal vasculogenesis in physiological and pathological neovascularization. Circ Res. 1999, 85: 221-228.View ArticlePubMedGoogle Scholar
- Reyes M, Dudek A, Jahagirdar B: Origin of endothelial progenitors in human postnatal bone marrow. J Clin Invest. 2002, 109: 337-346.View ArticlePubMedPubMed CentralGoogle Scholar
- Van Heek M, Compton DS, France CF, Tedesco RP, Fawzi AB, Graziano MP, Sybertz EJ, Strader CD, Davis HR: Diet-induced obese mice develop peripheral, but not central, resistance to leptin. J Clin Invest. 1997, 99: 385-390.View ArticlePubMedPubMed CentralGoogle Scholar
- Yip HK, Chang LT, Chang WN, Lu CH, Liou CW, Lan MY, Liu JS, Youssef AA, Chang HW: Level and value of circulating endothelial progenitor cells in patients after acute ischemic stroke. Stroke. 2008, 39: 69-74.View ArticlePubMedGoogle Scholar
- Chang HW, Leu S, Sun CK, Hang CL, Youssef AA, Hsieh YK, Yang CH, Cheng CI, Chen SM, Chen CJ, Chua S, Chang LT, Wu CJ, Yip HK: Level and value of circulating endothelial progenitor cells in patients with acute myocardial infarction undergoing primary coronary angioplasty: In vivo and in vitro studies. Transl Res. 2010, 156: 251-263.View ArticlePubMedGoogle Scholar
- Sun CK, Chang LT, Sheu JJ, Chiang CH, Lee FY, Wu CJ, Chua S, Fu M, Yip HK: Bone marrow-derived mononuclear cell therapy alleviates left ventricular remodeling and improves heart function in rat-dilated cardiomyopathy. Crit Care Med. 2009, 37: 1197-1205.View ArticlePubMedGoogle Scholar
- Yen CH, Lau YT: Vascular responses in male and female hypertensive rats with hyperhomocysteinemia. Hypertension. 2002, 40: 322-328.View ArticlePubMedGoogle Scholar
- Meyers MR, Gokce N: Endothelial dysfunction in obesity: etiological role in atherosclerosis. Curr Opin Endocrinol Diabetes Obes. 2007, 14 (5): 365-369.View ArticlePubMedGoogle Scholar
- Campia U, Tesauro M, Cardillo C: Human obesity and endothelium-dependent responsiveness. Br J Pharmacol. 2012, 165: 561-573.View ArticlePubMedPubMed CentralGoogle Scholar
- Powell BD, Redfield MM, Bybee KA, Freeman WK, Rihal CS: Association of obesity with left ventricular remodeling and diastolic dysfunction in patients without coronary artery disease. Am J Cardiol. 2006, 98: 116-120.View ArticlePubMedGoogle Scholar
- Chadha DS, Gupta N, Goel K, Pandey RM, Kondal D, Ganjoo RK, Misra A: Impact of obesity on the left ventricular functions and morphology of healthy asian indians. Metab Syndr Relat Disord. 2009, 7: 151-158.View ArticlePubMedGoogle Scholar
- Gong HP, Tan HW, Fang NN, Song T, Li SH, Zhong M, Zhang W, Zhang Y: Impaired left ventricular systolic and diastolic function in patients with metabolic syndrome as assessed by strain and strain rate imaging. Diabetes Res Clin Pract. 2009, 83: 300-307.View ArticlePubMedGoogle Scholar
- Barton M, Baretella O, Meyer MR: Obesity and risk of vascular disease: importance of endothelium-dependent vasoconstriction. Br J Pharmacol. 2012, 165: 591-602.View ArticlePubMedPubMed CentralGoogle Scholar
- Martins MA, Catta-Preta M, Mandarim-de-Lacerda CA, Aguila MB, Brunini TC, Mendes-Ribeiro AC: High fat diets modulate nitric oxide biosynthesis and antioxidant defence in red blood cells from c57bl/6 mice. Arch Biochem Biophys. 2010, 499: 56-61.View ArticlePubMedGoogle Scholar
- Md Muhibur R, Zac V, Moorhead John F: Paradoxical increase in nitric oxide synthase activity in hypercholesterolaemic rats with impaired renal function and decreased activity of nitric oxide. Nephrol Dial Transplant. 2011, 16: 262-268.Google Scholar
This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.