Aortic vasorelaxation and nitric oxide (NO) release (n = 6). A) NO release from endothelial cells of aorta was significantly higher in obesity than in normal control prior to LPS treatment. NO-release was remarkably impaired in obesity and more remarkably impaired in normal control after LPS treatment. * vs. † vs. ‡ vs. §, p < 0.0001. B) the KCl-induced vasoconstriction did not differ in obesity as compared with normal control regardless with and without LPS treatment. C) Phenylephrine (PE)-induced vasoconstriction was notably reduced in obesity as compared with normal control prior to LPS treatment (p < 0.01). Additionally, after the LPS treatment, the responsibility of PE-induced vasoconstriction was more prominent in normal control than in obesity (p < 0.001). D) Ach-induced vasorelaxation was significantly lower in normal control than in obesity prior to LPS treatment and more significantly lower in normal control than in obesity after LPS treatment (all p values < 0.001). * vs. † vs. ‡ vs. §, p < 0.0001. Symbols (*, †, ‡, §) indicate significant difference (at 0.05 level) followed by Bonferroni’s multiple-comparisons post hoc test.