Sirius red stain and Masson's Trichrome Stain of left ventricular (LV) myocardium (n = 8). C) The results of Sirius red staining (100x) demonstrated that the collagen-fiber deposition area (yellow-dot lines) in LV myocardium was significantly higher in obesity (B) than in normal control (A). F) The results of Masson's trichrome staining (100x) showed significantly higher fibrotic area in obesity (E) than in normal control (D). * vs. normal, p < 0.0001. Scale bars in left lower corner represent 100 μm. HPF = high-power field.