IHC staining (400x) for number of Flk-1+ stained cells in endothelial layer of aorta (n = 6). E) Demonstrating the number of Flk-1+ stained cells (black arrows) was similar between normal control (A) and obesity (B) prior to LPS treatment. However, the number of this biomarker in endothelial layer of aorta was significantly higher in normal control (C) than in obesity (D) after LPS treatment. Statistical analysis by one-way ANOVA. * vs. † vs. ‡, p < 0.0001. Symbols (*, †, ‡) indicate significant difference (at 0.05 level) followed by Bonferroni’s multiple-comparisons post hoc test. L = lumen side. Scale bars in left lower corner represent 20 μm. HPF = high-power field.