IHC staining (400x) for number of C-kit + stained cells in endothelial layer of aorta (n = 6). E) Demonstrating the number of C-kit + stained cells (black arrows) was significantly higher in normal control (A) than in obesity (B) prior to LPS treatment, more significantly higher in normal control (C) than in obesity (D) after LPS treatment. Statistical analysis by one-way ANOVA. * vs. † vs. ‡ vs. §, p < 0.0001. Symbols (*, †, ‡, §) indicate significant difference (at 0.05 level) followed by Bonferroni’s multiple-comparisons post hoc test. L = lumen side. Scale bars in left lower corner represent 20 μm. HPF = high-power field.