IHC staining (400x) for number of Sca-1+ stained cells in endothelial layer of aorta (n = 6). E) Demonstrating the number of Sca-1+ stained cells (black arrows) was significantly higher in normal control (A) than in obesity (B) prior to LPS treatment, more remarkably higher in normal control (C) than in obesity (D) after LPS treatment. Statistical analysis by one-way ANOVA. * vs. † vs. ‡ vs. §, p < 0.0001. Symbols (*, †, ‡, §) indicate significant difference (at 0.05 level) followed by Bonferroni’s multiple-comparisons post hoc test. L = lumen side. Scale bars in lower or upper corner represent 20 μm. HPF = high-power field.