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Caprin-1 plays a role in cell proliferation and Warburg metabolism of esophageal carcinoma by regulating METTL3 and WTAP

Journal of Translational Medicine volume 21, Article number: 159 (2023) Cite this article

Abstract

Background

Cytoplasmic activation/proliferation-associated protein-1 (Caprin-1) is implicated in cancer cell proliferation and tumorigenesis; however, its role in the development of esophageal carcinoma (ESCA) has not been examined.

Methods

Biological methods and data analysis were used to investigate the expression of Caprin-1 in ESCA tissue and cell lines. We comprehensively analyzed the mRNA expression and prognostic values, signalling pathways of CAPRIN1 in ESCA using public databases online. Biological functions of CAPRIN1 were performed by clorimetric growth assay, EdU staining, colony formation, flow cytometry, apoptosis analysis, Western blot, lactate detection assay, extracellular acidification rates. The underlying mechanism was determined via flow cytometric analysis, Western blot and rescue experiments. In addition, xenograft tumor model was constructed to verify the phenotypes upon CAPRIN1 silencing.

Results

Caprin-1 expression was significantly elevated in both ESCA tumor tissues and cell lines compared with that in normal adjacent tissues and fibroblasts. Increased CAPRIN1 mRNA expression was significantly associated with clinical prognosis and diagnostic accuracy. The GO enrichment and KEGG pathway analysis CAPRIN1 might be related to immune-related terms, protein binding processes, and metabolic pathways. A significant positive correlation was observed between high Caprin-1 protein levels and lymph node metastasis (P = 0.031), ki-67 (P = 0.023), and 18F- FDG PET/CT parameters (SUVmax (P = 0.002) and SUV mean (P = 0.005)) in 55 ESCA patients. At cut-off values of SUVmax 17.71 and SUVmean 10.14, 18F- FDG PET/CT imaging predicted Caprin-1 expression in ESCA samples with 70.8% sensitivity and 77.4% specificity. In vitro and in vivo assays showed that Caprin-1 knockdown affected ESCA tumor growth. Silencing Caprin-1 inhibited ESCA cell proliferation and glycolysis, and decreased the expression of methyltransferase-like 3 (METTL3) and Wilms’ tumor 1-associating protein (WTAP). However, this effect could be partially reversed by the restoration of METTL3 and WTAP expression.

Conclusions

Our data suggest that Caprin-1 could serve as a prognostic biomarker and has an oncogenic role in ESCA.

Background

Esophageal carcinoma (ESCA) is a common malignant cancer worldwide, especially in China [1], and has two main histological subtypes: esophageal squamous cell carcinoma (ESCC) (accounting for 90% of total ESCA cases) and esophageal adenocarcinoma (EA). Despite significant advances in ESCA diagnosis, prognosis, and treatment, the 5-year survival rate of patients remains unsatisfactory [2]. Therefore, new biological or pathological biomarkers and disease-specific molecular mechanisms underlying ESCA progression are urgently needed. Bioinformatic analysis and experimental approaches have been combined to explore novel predictive and prognostic markers for diverse cancers [3, 4].

The Warburg effect, also known as aerobic glycolysis, is metabolic hallmark of cancer cells [5]. Positron emission tomography/computed tomography (PET/CT), using a glucose analogue named 18F-fludeoxyglucose (18F-FDG), could reflect the glycolysis levels of tumors and has been widely used for early diagnosis, staging, and treatment assessment of ESCA [6, 7]. The 18F-FDG uptake rate was quantified by PET metabolic parameters like the maximum and mean standardized uptake value (SUVmax, SUVmean), metabolic tumor volume (MTV), and total lesion glycolysis (TLG). Tumour standardised uptake value (SUV) has been shown to be a useful PET metabolic parameter for risk assessment in cancers. PET metabolic parameters have been reported to be significantly associated with prognostic biomarkers expression in different tumors both in vitro and in vivo [8,9,10,11].

Cytoplasmic activation/proliferation-associated protein-1 (Caprin-1) [12], is involved in neurodegenerative diseases [13, 14] and various cancers [15,16,17,18]. Caprin-1 acts as RNA binding protein and participates in extensive biological and physiological processes, such as cell proliferation, RNA modification [19], and immune response [20]. N6-methylandenosine (m6A) is the most abundant modification in mammalian mRNA, and it plays important roles in cancer development [21]. Integrative network analysis has identified Caprin-1 as m6A regulator that selectively promotes methylations of m6A sites through physical interactions with m6A writers, such as methyltransferase-like 3 (METTL3) and methyltransferase-like 14 (METTL14) [19]. METTL3, METTL14, and Wilms’ tumor 1-associating protein (WTAP) were identified as components of the human m6A methyltransferase complex, and WTAP interacts with METTL3 and METTL14 [22, 23]. However, disease-relevant expression profiles and biological functions of Caprin-1, and its correlations with the hallmark of ESCA have not yet been elucidated.

In the present study, we investigated the relationship between the expression and the prognosis value of Caprin-1, as well as its potential mechanisms of action in ESCA, through a comprehensively bioinformatics analysis of data from The Cancer Genome Atlas (TCGA) [4] and Gene Expression Omnibus (GEO) [24]. The experimental methods and correlation analysis of Caprin-1 with 18F-FDG PET parameters in ESCA were combined to explore mechanisms underlying the functions of Caprin-1 in tumor growth and glycolysis reprogramming. Our results indicate that Caprin-1 could be a novel diagnostic and prognostic biomarker for patients with ESCA.

Methods

Patient samples

The study respectively reviewed data from 55 patients who were surgically treated and had pathologically confirmed ESCC in Taihe Hospital from January 2018 to July 2020. All patients met the following inclusion criteria: (a) they underwent 18F-FDG PET/CT imaging prior to chemotherapy or radiation therapy; (b) their tissue samples were available for immunohistochemistry (IHC) staining; and (c) their complete demographic information, including gender, age, tumor size, pathological type, histological differentiation, lymph node metastasis, and pathological stage ( p stage), was available. Another 14 paired samples, including tumor tissues and adjacent normal tissues from patients with ESCC were collected for Western blot experiments.

18F-FDG PET/CT imaging and data analysis

Glucose metabolism imaging was performed on a 18F-FDG PET/CT system (Biograph mCT-64; Siemens Healthcare, Erlangen, Germany). As described previously [10, 11], all patients fasted for at least 6 h, but had free access to water until the start of imaging. Whole-body position was monitored 50 min after intravenous FDG administration with a dose of 3.7–4.1 MBq/kg and lasted for approximately 15 min. PET/CT images were obtained according to the manufacturer’s protocol [23]. Metabolic parameters of 18F-FDG PET images were visually evaluated by two experienced nuclear medicine physicians blinded to the final clinical diagnosis. Briefly, a region of interest was placed around the primary tumor; FDG uptake in lesions was determined for calculation of PET parameters.

IHC and immunofluorescence stain assay

IHC staining of esophagus tissue was employed to investigate protein expression as previously described [10, 11]. Samples were dissected into 5-μm-thick tissue sections embedded in paraffin, and incubated with antibodies against Caprin-1 (ProteinTech Group, Chicago, IL, USA), and Ki-67 (Zhongshan Golden Bridge Biotechnology, Beijing, China). All data were analyzed by two independent pathologists blinded to patient information. For Ki-67 evaluation, we considered the percentage of nuclear staining for scoring proliferative status. Ki-67 proliferation index ≥ 10% was defined as Ki-67 positive. Ki-67/Caprin-1 protein expression was determined according to the staining intensity score: 0 (no staining), 1 (weak staining, light yellow), 2 (moderate staining, yellow brown), and 3 (strong staining, brown). Final intensity scores of < 2 and ≥ 2 were considered low and high expression, respectively. The clinical patients were divided into Caprin-1/Ki-67 low and high groups based on their IHC paraffin staining score ranking.

For immunofluorescence staining, cultured cells on slides were washed, fixed and stained with Caprin-1 primary antibody and with Donkey anti-goat Alexa Fluro 488 (Abcam, Cambridge, England) as previously reported [25, 26]. Next, slides were mounted with DAPI (Thermo Fisher Scientific, Waltham, MA, USA) to stain cell nuclei and observed under a confocal fluorescence microscope (LEICA TCS SP8; Leica Microsystems, Wetzlar, Germany).

Gene expression pattern and survival prognosis analysis in public datasets

The mRNA expression profiles (HTSeq counts) and associated clinical data of the CAPRIN1 in ESCA patients and cell lines were obtained from the TCGA website and the GEO database (GSE63941, GSE69925). Expression profiles were plotted in the ggplot2 R. The TIMER database [27] was also used for mRNA expression analysis. Survival curve analysis was performed to assess the correlation between the expression of different genes and survival rates, in the Kaplan–Meier plots database [28]. Area under curve was used for analysis of prognostic or predictive accuracy. The “pROC” package in R was used to generate the receiver operating characteristic (ROC) curve, which allowed for time-dependent analysis with the censored data of TCGA cancers.

Function and network analysis using Cytoscape and gene-set enrichment analysis (GSEA)

The co-expression genes of CAPRIN1 in ESCA were downloaded from LinkedOmics database, wherein multi-omics data were analyzed across 32 TCGA cancer types [29]. Subsequently, gene ontology (GO) data for BP (biological process), CC (cellular component), and MF (molecular function) of co-expressed genes were investigated. Next, the top 100 correlated genes were screened out for the Protein-protein interaction (PPI) networks. PPI data were obtained from the String database (https://string-db.org/, version 11.5, updated on August 12, 2021). The top 20 hub genes were identified by degree using a plugin named cytoHubba in Cytoscape.

GSEA was also performed using the GEO matrix of the GSE69925 cohort to investigate the potential role of CAPRIN1 in the high- and low-expression groups based on C5 (Gene Ontology, GO) and C2 [Canonical pathways, Kyoto Encyclopedia of Genes and Genomes (KEGG)]. Investigated glycolysis signatures included REACTOME_GLYCOLYSIS, HALLMARK_GLYCOLYSIS, KEGG_GLYCOLYSIS, and the MOOTHA_GLYCOLYSIS. These gene sets were downloaded from the Molecular Signatures Database (MSigDB). False discovery rate < 0.25 and nominal P < 0.05 denoted the cutoff for significance.

Cell culture and treatment

Human ESCC cell lines Eca109 and KYSE150 were the kind gift of Prof. Luo (Hubei University of Medicine, China) and were mycoplasma-free. Eca109 cells were cultured in DMEM (Hyclone Laboratories Inc, Logan, UT, USA), and KYSE150 cells were maintained in RPMI 1640 (Hyclone Laboratories Inc). The medium was supplemented with 10% fetal bovine serum (Thermo Fisher Scientific). Small interfering RNA (siRNA, 100 pmol) against human CAPRIN1 was transfected into cells with Lipofectamine 8000 transfection reagent (Beyotime Biotechnology, China), while a non-specific siRNA was used as a negative control. The siRNA sequences were as follows: Caprin-1 siRNA 1# (siCaprin-1 1#), sense 5′-CCAGGAAGUCACAAAUA AUTT-3′; antisense 5′-AUUAUUUGUGACUUCCUGGTT-3′; Caprin-1 siRNA 2# (siCaprin-1 2#), sense 5′-GGGACCUGCUGGAAGGGAATT-3′; antisense 5′-UUCCCUUCCAGCAGGUCCCTT-3′; siNC, sense 5′-ACGUGACACGUUCGGAG AA-3′; antisense 5′-UCUUCUCCGAACGUGUCACGU-3′. Eca109 cells were transfected with Caprin-1 knockdown lentivirus (termed as shCaprin-1) to select stably transfected hybrid colonies; and transfected with plasmid overexpressing METTL3 or WTAP (METTL3 OE or WTAP OE) generated by Genechem.

Real-time quantitative PCR and Western blot

For mRNA analysis, total RNA extraction for cDNA synthesis was conducted with the Taqman Reverse Transcription Reagent kit (TaKara-Bio, Kusatsu, Japan). Real-time quantitative PCR was conducted with different primer sequences (Table 1) using SYBR Green Master Mix (TaKaRa).

Table 1 Primer sequences for real time RT-PCR
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Proteins were separated by SDS–PAGE followed by Western blot as described previously [30, 31]. Anti- GAPDH (Cell Signaling Technology), anti-Caprin-1 (116 kDa, ProteinTech Group), anti-HIF1α (120 kDa, ProteinTech Group), anti-c-Myc (49 kDa, roteinTech Group), anti-WTAP (45 kDa, Santa Cruz Biotechnology), and anti-METTL3 (64 kDa, Abcam) were used as primary antibodies. HRP-conjugated anti-mouse and anti-rabbit IgG (Cell Signaling Technology) were used as secondary antibodies.

Cell proliferation assay

Cells were maintained in 96-well plates (5 × 103 cells/well) for 24 h and transfected with siRNA. Cell viability and growth rate were assessed using CellTiter 96 AQueous One Solution Cell Proliferation Assay (MTS) kit (Promega, Madison, WI, USA) following the manufacturer’s instructions. Cell proliferation was assessed using the BeyoClick™ EdU-488 assay kit (Beyotime). Briefly, 3 × 104 cells were seeded in 24-well cover-glass bottom plates, incubated for 24 h, and transfected with siRNA. Then the cells were treated with 10 μM Edu for 2 h, fixed, permeabilized, and stained with the Click Additive Solution. Cell nuclei were stained with DAPI. Images were captured using a Leica SP8 laser-scanning confocal microscope. The cell proliferation rate was calculated as a percentage of EdU-positive cells per DAPI-positive cells. For the colony formation assay, cells were seeded into 12-well plates and treated with siRNA. After 14 days, visibly stained colonies were counted using Image J software.

Apoptosis detection

Cell apoptosis was assessed by flow cytometry (BD FACS Vantage SE, Franklin Lakes, NJ, United States) using Apoptosis Detection Kit I (BD Biosciences Pharmagen, San Diego, CA, USA) according to the manufacturer’s instructions. Treated cells binding only to Annexin V were classified as early apoptotic, while double-stained cells were classified as late apoptotic [31].

Lactate detection assay

To measure lactate production, Eca109 cells were seeded in 96-well plates (8 × 103 cells/well) in triplicate. The culture medium was collected to measure lactate concentration as determined by a lactate assay kit (Eton Bioscience Inc, San Diego, CA, USA) 48 h after siRNA transfection.

Extracellular acidification rates (ECARs)

ECARs were measured using an XF24 extracellular analyzer (Seahorse Bioscience, North Billerica, MA, USA) [32]. Briefly, 20,000 Eca109 cells/well were cultured in the XF24 cell culture plate with medium at 48 h after siRNA transfection. Cells were washed with PBS, the respective XF assay medium with 2 mM glutamine was added to each well, and the plate was incubated at 37 °C for approximately 45 min. After analyzer calibration, sequential compound injections, including glucose, oligomycin A and 2-DG (AmyJet Scientific, Wuhan, China), were applied to test glycolytic activity.

Animal model

For the in vivo xenograft implantation assay, male BALB/c athymic nude mice (five mice per group) were subcutaneously injected with stable Caprin-1 knockdown Eca109 cells. Tumor volumes were measured twice every week, and calculated using the following formula: volume (mm3) = longest diameter × shortest diameter2/2. After 5 weeks, animals were sacrificed and tumors were removed for further examination.

Statistical analysis

Statistical analysis were conducted using SPSS Statistics for Windows, version 16.0 (SPSS Inc., Chicago, IL, USA) and R package version (4.0.3). Significance levels were set at P < 0.05. Survival and ROC analyses were performed in R or in the corresponding R packages survival; survminer, and pROC. Unpaired two-tailed Student’s t tests were applied to compare data between two groups, and one-way ANOVA was used for multiple comparisons (CAPRIN1 expression in patient with differing race and histological types, in vitro and in vivo expiments). Proportional differences in clinicopathological characteristics were analyzed by chi-squared. For comparisons of medians in groups (PET metabolic parameter TLG and MTV), nonparametric statistical tests (Kruskall-Wallis test) were used. Spearman’s rho test was used to evaluate correlations between PET parameters and CAPRIN1 expression in ESCA patients. Multivariate Cox regression analysis was applied to identify the predictor for positive Caprin-1 expression. The greatest Youden index (sensitivity + specificity –1) was used to depict the optimal cutoff value for the ROC curve. To investigate the correlation between the expression of Caprin-1 and m6A-related genes in the TCGA data set, 20 m6A-related genes [21] were derived from previous work. All aforementioned analyse and R packages were implemented using R Foundation for Statistical Computing (2020) version 4.0.3 and software packages, including ggplot2 pheatmap.

Results

Clinicopathological characteristics of Caprin-1 in ESCA patients

The mRNA and protein expression of CAPRIN1 was up-regulated in ESCA tissues than in normal tissues (P < 0.05; Fig. 1A and B). Similarly, Western blot analysis confirmed the results in the collected tissues (P < 0.05, Fig. 1C). We observed high CAPRIN1 levels in several ESCA cell lines, especially in KYSE30 and KYSE70, compared with fibroblast cultured from esophageal squamous cell carcinoma tissue using the GEO database (GSE63941, Fig. 1D).

Fig. 1
figure 1

Expression pattern and overall survival (OS) rates of CAPRIN1 in ESCA patients and cell lines. A Comparison of CAPRIN1 expression among EA, ESCC, and normal tissues based on data from the TCGA-ESCA cohort. B Immunohistochemical staining and quantification of Caprin-1 protein in ESCC tissues and adjacent normal tissues. C The protein expression level of Caprin-1 in 14 paired ESCC tissues was examined using Western blot. T, tumor; N, adjacent normal tissues. D CAPRIN1 mRNA expression levels in different ESCA cell lines and fibroblast cultured from esophageal squamous cell carcinoma tissue in the GEO cohort (GSE63941). E OS analysis according to the mRNA expression of CAPRIN1 in ESCC and EA F patients using a Kaplan–Meier plotter. G The ROC curve of CAPRIN1 for the diagnosis of patients with ESCA. * P < 0.05, ** P < 0.01, *** P < 0.001

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Clinicopathological parameters of the 55 patients with ESCC are presented in Table 2. Caprin-1 expression in ESCC patients was significantly associated with lymph node metastasis (P = 0.031), Ki-67 expression (P = 0.023), SUVmax (P = 0.002), and SUVmean (P = 0.005), but not with gender, age, tumor size, differential status, p stage, TLG or MTV. But CAPRIN1 mRNA expression was significantly negatively associated with age (P < 0.05), not with races including in ESCA and ESCC tumors (Additional file 1: Fig. S1A and B). Caprin-1 expression was negatively associated with OS in 81 ESCC patients (HR = 0.30, 95% CI: 0.11–0.81, P = 0.012; Fig. 1E) and was significantly positively associated with OS in 80 EA patients (HR = 2.48, 95% CI: 1.28–4.81, P = 0.006; Fig. 1F) in TCGA database. Finally, CAPRIN1 showed high diagnostic value in ESCA (area under the curve = 0.836; Fig. 1G). Collectively, these results suggest that Caprin-1 was up-regulated in ESCA and could serve as a potential diagnostic indicator for patients with ESCA.

Table 2 Clinicopathological characteristics of 55 patients
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Immune-related terms and metabolic pathways significantly enriched for CAPRIN1 mRNA expression

To further explore the underlying mechanism, a group of genes correlated positively and negatively with CAPRIN1 expression were screened (Fig. 2A). The interactions between 156 genes are shown in Additional file 1: Fig. S1C. The top 20 hub genes were screened and ranked by the number of nodes, such as XPO1, NUP214, NUP155, NUP153 (Fig. 2B). Differentially expressed genes were highly associated with metabolic process, membrane, and protein binding process (Additional file 1: Fig. S1D). Significant differences (false discovery rate < 0.25, NOM P -value < 0.05) in the enrichment of GO and KEGG collection in 85 ESCA patients (Fig. 2C, D). Thus, overall functions of DEGs seemed to map on immune-related GO terms, such as antigen processing and presentation of peptides or polysaccharide antigens via MHC class II, membrane, and protein binding processes such as clathrin adaptor complex and coat. The KEGG enrichment analysis indicated the most significantly enriched signaling pathways, such as primary metabolism, RNA polymerase and pathogenic Escherichia coli infection.

Fig. 2
figure 2

Differentially expressed genes and gene set enrichment analysis (GSEA) with high and low CAPRIN1 expression groups. A The heat map of the top 25 genes positively (red) and negatively (blue) correlated with CAPRIN1 in the TCGA-ESCA database. B The top 20 hub genes screened in the PPI network using the cytoHubba module of Cytoscape (higher color represents stronger connectivity). C The most significantly enriched GO annotations and KEGG pathways D of CAPRIN1 in the GEO database (GSE69925) by GSEA

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Suppression of Caprin-1 inhibited ESCA cell proliferation by inhibiting glycolysis

Western blotting and immunofluorescence assay (Fig. 3A, B) revealed that both siRNA 1# and siRNA 2# resulted in a significant down-regulation of Caprin-1 protein expression in Eca109 and KYSE150 cells. Caprin-1 expression was primarily localized to the cytoplasm of Eca109 cells (Fig. 3B). Caprin-1 knockdown in Eca109 and KYSE150 cells suppressed cell growth (Fig. 3C). The EdU assay showed that Caprin-1 knockdown resulted in 30% decreased proliferation in Eca109 cells (Fig. 3D, E). Colony formation assay revealed that Caprin-1 siRNA inhibited colony formation potential of both cells (Fig. 3F, G). Caprin-1 siRNA induced early apoptosis of Eca109 cells (Fig. 3H). These results indicated that Caprin-1 knockdown could inhibit ESCA cell proliferation.

Fig. 3
figure 3

Down-regulation of Caprin-1 inhibited cell proliferation and induced apoptosis. A Western blot showing effective knockdown of Caprin-1 protein in two ESCA cell lines. B Immunofluorescence staining of Caprin-1 in Eca109 cells. C Colorimetric growth assay in Eca109 cells and KYSE150 cells transfected with Caprin-1 siRNAs or control oligos. D Representative images and quantification E of Edu positive rate in Eca109 cells. F Colony formation assay and quantification G in Eca109 cells and KYSE150 cells. H Flow cytometry analyses of cell apoptosis rate in Eca109 cells

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GSEA results showed that the four glycolysis gene signatures were enriched in samples with high Caprin-1 expression. REACTOME_GLYCOLYSIS was significantly enriched at nominal p-value < 0.05 (Fig. 4A). We also observed significantly decreased ECAR and lactate release in Caprin-1 knockdown cells than in negative controls (Fig. 4B, C). Caprin-1 was positively correlated with the expression of NUP160 (r = 0.770, P < 0.001), NUP133 (r = 0.570, P < 0.001), NUP155 (r = 0.550, P < 0.001), and NUP214 (r = 0.550, P < 0.001) in TCGA-ESCA samples (Fig. 4D). Meanwhile, Caprin-1 was positively correlated with the expression of HIF1A and MYC (P < 0.05) (Fig. 4E). Western blot further confirmed that HIF1α and c-Myc expression was elevated in ESCA tissues than in paracancerous and normal tissues, and in the same pattern with that of Caprin-1 (Fig. 4F). After silencing Caprin-1 in the ESCA cells, HIF1α and c-Myc expression decreased (Fig. 4G). Eca109 cells transfected with siCaprin-1 had significantly decreased mRNA expression of glycolysis related genes, SLC2A1, HK2, HIF1A, MYC, NUP160, NUP133, and NUP155, compared to those transfected with control siRNA (Fig. 4H).

Fig. 4
figure 4

Knockdown of Caprin-1 inhibited glycolytic metabolism through the down-regulation of SLC2A1, HK2, HIF1A, MYC, NUP160, NUP133, and NUP155. A Gene-set enrichment analysis (GSEA) of the interrogated glycolysis signatures between the high and low CAPRIN1 groups in the database (GSE69925); NES, normalized enrichment score. B ECAR were measured in Eca109 cells 48 h after Caprin-1 knockdown using the Seahorse XF24 extracellular flux analyzer. C Lactate production was assessed in Eca109 cells after 48 h of siRNA transfection. D Correlation between CAPRIN1 and the top four leading-edge genes contributing to GSEA in the TCGA database. E Correlation between CAPRIN1 and glycolysis genes (HIF1A and MYC) in the TCGA database. F The protein expression level of HIF1α, c-Myc, and Caprin-1 in 7 paired ESCC tissues was examined using Western blot. T, tumor; N, adjacent normal tissues. G The expression patterns of HIF1α and c-Myc in Eca109 cells after silencing Caprin-1. H Changes in mRNA levels were detected by real time PCR after the Caprin-1 1# knockdown of Eca109 cells

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Relationship between Caprin-1 expression and glucose metabolic asymmetries

To further explore the role of Caprin-1 expression on glucose metabolism in ESCA, the potential association between 18F-FDG PET/CT metabolic parameters and Caprin-1 expression was assessed in 55 tumour samples. Two typical PET images and IHC staining for Caprin-1 of two case are shown in Fig. 5A. SUVmax and SUVmean were significantly different between groups according to Caprin-1 expression (Table 3). SUVmax, SUVmean, and TLG were positively correlated in a linear correlaion with IHC intensity levels of Caprin-1 (rho = 0.538, 0.540, and 0.390, respectively; P < 0.01), while the Caprin-1 intensity score showed no statistical correlation with MTV (Fig. 5B–E). Multivariate analysis results are shown in Table 4. Lymph node metastasis (P = 0.022) and SUVmax (P = 0.048) were strongly correlated with Caprin-1 expression. The area under the SUVmax, SUVmean, TLG, and MTV ROC curves was 0.813, 0.814, 0.727, and 0.626, respectively. Sensitivity of both SUVmax and SUVmean to predict Caprin-1 expression was 70.8% and their specificity was 77.4% (Fig. 5F). Therefore, SUVmax of 17.71 or SUVmax of 10.14 were suitable critical values for the prediction of Caprin-1 expression in ESCA.

Fig. 5
figure 5

High expression of Caprin-1 was correlated with high glycolytic activity based on metabolic parameters in 18F-FDG PET imaging. A Representative images of PET/CT imaging and immunohistochemical staining for Caprin-1 in ESCC patients with high SUVmax (left) and low SUVmax (right). B The TLG, MTV C, SUVmax D, and SUVmean E showed a linear correlation with the protein levels of Caprin-1 with correlation coefficients of 0.390, 0.216, 0.538, and 0.540 respectively. F The determination of the cutoff value of SUVmax, SUVmean, TLG, and MTV by the receiver operating characteristics (ROC) curve

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Table 3 Comparison of PET metabolic parameter according to Caprin-1 expression
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Table 4 Multivariate analysis of Caprin-1 expression status in patients with ESCA
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Silencing Caprin-1 delayed ESCA progression by downregulating METTL3 and WTAP expression

Compared with the CAPRIN1-low expression group (n = 81), the CAPRIN1-high expression group (n = 81) showed statistically significant correlation with all m6A-related genes except ZC3H13 and YTHDC1 in TCGA-ESCA (P < 0.05) (Fig. 6A). The mRNA expression of METTL3, WTAP, VIRMA and RBM15 increased in the ESCA samples (Fig. 6B). CAPRIN1 expression had a significantly positive correlation with m6A writers in ESCA, including METTL3 (r = 0.330, P < 0.001) and WTAP (r = 0.250, P = 0.001) (Fig. 6C). After silencing Caprin-1 in the ESCA cells, METTL3 and WTAP expression in ESCA cells decreased (Fig. 6D–E).

Fig. 6
figure 6

Caprin-1 expression was significantly correlated with WTAP and METTL3 in ESCA. A Comparison of m6A related genes in TCGA-ESCA tumors with high or low CAPRIN1 expression. B Expression pattern of m6A writers in ESCA tumor tissue and normal tissues as shown in the TCGA database. C The co-expression of CAPRIN1 with METTL3 and WTAP in ESCA from the TCGA database. D The expression patterns of METTL3 and WTAP in Eca109 cells after silencing Caprin-1 were determined by qRT-PCR and Western blot assay E, F

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The expression of METTL3 and WTAP in ESCA cells was reverted by METTL3 and WTAP overexpression, compared to the shCaprin-1 group (Fig. 7A). Proliferation and lactate release was suppressed in ESCA cells upon Caprin-1 silencing; however, the restoration of METTL3 and WTAP partially reversed these effects in a significantly manner (P < 0.05, Fig. 7B and C). WTAP OE could rescue the Caprin-1 shRNA-induced decreased in mRNA expression of glycolysis related genes (SLC2A1, HK2, HIF1A, MYC, NUP160, NUP133, and NUP155); METTL3 OE could rescue the mRNA expression of SLC2A1, HK2, NUP160, NUP133, and NUP155 (Fig. 7D). METTL3 or WTAP overexpression could reverse the inhibitory effect of Caprin-1 silencing on the proliferation and glycolysis of ESCA cells.

Fig. 7
figure 7

Overexpression of METTL3 and WTAP rescued the cell proliferation phenotype induced by silencing Caprin-1. A The mRNA and proteins of METTL3 and WTAP in Eca109 cells upon Caprin-1 silencing or combined with METTL3 and WTAP overexpression. B The cell viability and lactate release C of Eca109 cells upon Caprin-1 silencing or combined with METTL3 and WTAP overexpression. D qRT-PCR analysis was applied in Eca109 cells using indicated PCR primers

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Knockdown of Caprin-1 inhibited tumour growth in vivo

Finally, to further investigate the impact of Caprin-1 on tumor growth in vivo, the subcutaneous xenograft tumor model was established in nude mice using Caprin-1 knockdown (shCaprin-1) or the control cells (shCtrl). As expected, tumor grew dramatically slower in shCaprin-1 group compared with the control group (P < 0.05) (Fig. 8A and B). Further studies showed that knockdown of Caprin-1 in vivo inhibited protein expression of Caprin-1, METTL3, and WTAP (Fig. 8C and D). Additionally, IHC analyses showed that a significant decrease in the positive rate of Ki67 in Caprin-1 knockdown group (Fig. 8E). Overall, these results demonstrated that Caprin-1 silencing could inhibit tumor growth in vivo.

Fig. 8
figure 8

The growth of ESCA xenografts is inhibited by silencing Caprin-1. A Representative images of transplanted tumors in nude mice injected with Eca109 cells. B Tumour growth curve of shCtrl and shCaprin-1 group (n = 5). C, D Western blot analysis of Caprin-1, METTL3, and WTAP expression in tumor tissues. E IHC analysis of Ki67 in xenografs. The histogram indicates the Ki67 positive cells. *P < 0.05, ***P < 0.001

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Discussion

Caprin-1 is up-regulated in different cancer types [33], including lung [15, 34], prostate [16], breast [17], gastric [35], liver [18, 36] and colon cancer [37]. No studies have reported the underlying biological function of Caprin-1 in ESCA. Herein, we found that CAPRIN1 mRNA expression was up-regulated and associated with poor prognosis and good diagnostic accuracy in ESCA. Increased levels of Caprin-1 mRNA and protein expression were associated with lymph node metastasis and PET metabolic parameters in ESCA patients. PET parameters (SUVmax and SUVmean) might be suitable predictors of Caprin-1 expression in ESCA patients. Additionally, CAPRIN1 might promote ESCA progression through glycolytic reprogramming and regulation of m6A writers (METTL3 and WTAP). Therefore, we identified Caprin-1 as a potential biomarker that modulates glucose metabolism and m6A writers in ESCA.

The absence of Caprin-1 leads to cancer cell proliferation, migration, and invasion defects [15, 17] and immune cells proliferation [20]. In this study, the GO enrichment and KEGG pathway analysis CAPRIN1 might be related to immune-related terms, protein binding processes, and metabolic pathways. Meanwhile, further verification experiments with IHC confirmed that Caprin-1 expression was significantly associated with Ki-67 expression (strictly associated with cell proliferation) in ESCC patients. The in vitro and in vivo results revealed that knockdown of Caprin-1 inhibited ESCC cell proliferation. While the clinical outcomes and pathophysiology of ESCA and OA seems to be different, Caprin-1 expression displayed different OS patterns in these cancers. The Warburg effect is associated with malignant features of cancers, and is measured by high glycolytic rates, increased lactate release, and several intermediates and enzymes for rapidly proliferating cells [38]. Recent studies have revealed that Caprin-1 expression is functionally required for glutamine metabolism [39], which also involves cell proliferation and metastasis in various cancers [40]. Kim et al. [12] showed that CAPRIN1 controls RNA processing and translation, which regulates human and mouse metabolism [41]. However, whether Caprin-1 expression participates in the regulation of glucose metabolism remains unclear.

Our results demonstrated that the glycolysis pathway was significantly enriched in response to high CAPRIN1 expression in ESCA patients. Consistently, Caprin-1 knockdown inhibited glycolysis, followed by down-regulating the commonly glycolytic genes (SLC2A1, HK2, HIF1A, and MYC) and glycolytic genes screened from the PPI network (nucleoporins NUP160, NUP155, and NUP133) in ESCA cells. Given that Nup160, Nup133 [42] and Caprin-1 [14] play key roles in mRNA export, the links among them warrants further investigation. The fact that CAPRIN1 can bind directly and selectively to c-Myc and HIF-α has been reported previously [43]. MYC activity interference has been implicated in many malignant phenotypes of human cancers, including the Warburg effect [44]. It has been proven that HIFα acts as a major mediator of transcription in response to hypoxia, and provokes cell proliferation and the Warburg effect [45]. Surov et al.[46] reported a significant correlation of HIF-α expression and Ki-67 levels with PET parameters in head and neck squamous cell carcinoma. The results of the present study corroborated those of previous reports and showed that Caprin-1 expression had a significantly positive association with 18F-FDG PET parameters, which can reflect the glucose metabolism and growth patterns of tumor cells in ESCA patients. Additionally, 18F-FDG uptake may have a role in predicting Caprin-1 expression. These results suggest that Caprin-1 promotes ESCA tumorigenesis by inducing the Warburg effect.

Caprin-1 plays critical roles in m6A modification and regulates m6A disruption in eukaryotic RNA [19, 47]. Using RNA chemical proteomics, Arguello et al. [47] revealed that the CAPRIN1, G3BP1/2, RBM42, and USP10 are repelled by the m6A modification. Our results showed a significantly positive correlation of CAPRIN1 with most m6A regulators in ESCA. Previous studies in 25 cell lines also showed that CAPRIN1 knockdown selectively modulated m6A sites within the coding regions through interactions with m6A writers (METTL3 and METTL14)[19]. Our results showed that the expression of m6A writers (METTL3, WTAP, VIRMA and RBM15) was significantly elevated in clinical ESCA tissues, whereas expression of METTL14, RBM15B, and ZC3H13 was not significantly changed. Among these, METTL3 promotes aerobic glycolysis and ESCC tumour development [23]. Interfering with METTL3 expression can reduce the level of m6A modification of HIF1α and inhibit the metabolic reprogramming of colorectal and liver cancer cells [48, 49]. The elevated expressions of WTAP and VIRMA genes were strongly correlated with poor prognosis in ESCA patients, augmented levels of WTAP were associated with poor OS of EA [50], whereas WTAP promoted the proliferation and migration of ESCC [51]. Both METTL3 and WTAP accelerates the Warburg effect of gastric cancer [52, 53]. We found that there were positive correlations among METTL3, WTAP, and CAPRIN1 in TCGA-ESCA tissues. Besides, CAPRIN1 knockdown inhibits ESCC cell proliferation and tumour growth and decreased the expression of METTL3 and WTAP. Therefore, we speculated that Caprin-1 might affect ESCA progression by affecting METTL3 and WTAP. To further validate this speculation, we conducted a functional rescue experiment. As expected, our validation experiments confirmed that Caprin-1 knockdown significantly inhibited the growth rate and lactate production of ESCA cells, which could be rescued by METTL3 and WTAP overexpression. The underlying mechanism is that Caprin-1 knockdown inhibited cell proliferation and metabolism through inhibiting the mRNA expression of glycolysis related genes (SLC2A1, HK2, and so on), which could also be rescued by METTL3 or WTAP overexpression. In keeping with our findings, previous studies have reported that METTL3 directly interacted and stabilized with HK2 and SLC2A1, then further activated the glycolysis pathway [53, 54]. Moreover, WTAP was found to be closely associated with HIF-1α and MYC [55, 56]. Thus, we conclude that Caprin-1 could affect the ESCA cell proliferation by up-regulating METTL3 and WTAP expression.

Conclusion

In conclusion, our preliminary findings showed that Caprin-1 was highly expressed in cancer tissues and significantly associated with prognosis in diverse cancer types. Caprin-1 might promote ESCA progression by regulating glycolysis reprogramming and m6A writers including METTL3 and WTAP. The Warburg effect, m6A RNA methylation, and the interaction between them are crucial for tumor progression [57]. Although in vitro studies have been conducted to explore the potential function of Caprin-1, the underlying mechanisms of signaling pathways in ESCC has not been fully elucidated, thus further investigation is required to confirm the role of Caprin-1 in ESCA growth and progression in patients, and its relationship with glycolysis and m6A methylation in the tumor microenvironment.

Availability of data and materials

Publicly available datasets were analyzed in this study. The datasets used and/or analyzed during the current study are available from the corresponding author on reasonable request.

Abbreviations

Caprin-1:

Cytoplasmic activation/proliferation-associated protein-1

ESCA:

Esophageal carcinoma

PET/CT:

Positron emission tomography/computed tomography

18F-FDG:

18F-fludeoxyglucose

ESCC:

Esophageal squamous cell carcinoma

EA:

Esophageal adenocarcinoma

m6A:

N6-methylandenosin

TCGA:

The Cancer Genome Atlas

GEO:

Gene Expression Omnibus

GSEA:

Gene-set enrichment analysis

METTL3:

Methyltransferase-like 3

WTAP:

Wilms’ tumor 1-associating protein

METTL14:

Methyltransferase-like 14

SUVmax:

Maximum Standardized Uptake Value

SUVmean:

Mean Standardized Uptake Value

MTV:

Metabolic tumor volume

TLG:

Total lesion glycolysis

ECARs:

Extracellular acidification rates

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Acknowledgements

We thanked Juan Xue (Hubei University of Medicine) for her kindly help in flow cytometry analysis, Li Xu (Hubei University of Medicine) for ECAR analysis, and Bin Wang (University of British Columbia) for his for technical assistances.

Funding

This work was supported by National Natural Science Foundation of China (No.81971060), Shaanxi Province Science Fund for Distinguished Young Scholars (2023-JC-JQ-59), the Hubei province’s Outstanding Medical Academic Leader program, the Foundation for Innovative Research Team (No. T2020025) and the general project (No. B2021160 and No. Q20222109) of Hubei Provincial Department of Education, and the Key Discipline Project of Hubei University of Medicine.

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  1. School of Pharmacy, Health Science Center, Xi’an Jiaotong University, Xi’an, 710061, People’s Republic of China

    Yan Gao & Yanmin Zhang

  2. Department of Nuclear Medicine and Institute of Anesthesiology and Pain, Department of Pathology, Taihe Hospital, Hubei University of Medicine, Shiyan, 442000, People’s Republic of China

    Yan Gao, Lingling Yuan, Changbin Ke, Zhijun Pei, Xiaobo Liu, Ruimin Wu & Xueyan Kui

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Contributions

Y.M.Z. and Y.G. contributions to the conception or design of the work; Y.G., L.L.Y., C.B.K., and Z.J.P. performed the acquisition, analysis, or interpretation of the data for the work; Y.G. wrote and revised the manuscript, Y.M.Z., X.B.L., R.M.W. and X.Y.K. drafted the work or revised it critically for important intellectual content. All authors have read and agreed to the published version of the manuscript. All authors read and approved the final manuscript.

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Supplementary Information

Additional file 1: Figure S1.

Bioinformatic analysis of CAPRIN1 mRNA expression. (A) Comparison of CAPRIN1 expression among age (> 60 and ≤ 60) and race (Asian, White, and Black or African American) (B) groups in the TCGA-ESCA and ESCC database. (C) PPI analysis of the CAPRIN1 correlated genes. (D) Enriched BP, CC, and MF GO terms in the differentially expressed genes in the LinkedOmics database.

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Gao, Y., Yuan, L., Ke, C. et al. Caprin-1 plays a role in cell proliferation and Warburg metabolism of esophageal carcinoma by regulating METTL3 and WTAP. J Transl Med 21, 159 (2023). https://doi.org/10.1186/s12967-023-04001-0

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