Fig. 3

Down-regulation of Caprin-1 inhibited cell proliferation and induced apoptosis. A Western blot showing effective knockdown of Caprin-1 protein in two ESCA cell lines. B Immunofluorescence staining of Caprin-1 in Eca109 cells. C Colorimetric growth assay in Eca109 cells and KYSE150 cells transfected with Caprin-1 siRNAs or control oligos. D Representative images and quantification E of Edu positive rate in Eca109 cells. F Colony formation assay and quantification G in Eca109 cells and KYSE150 cells. H Flow cytometry analyses of cell apoptosis rate in Eca109 cells