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Fig. 4 | Journal of Translational Medicine

Fig. 4

From: Caprin-1 plays a role in cell proliferation and Warburg metabolism of esophageal carcinoma by regulating METTL3 and WTAP

Fig. 4

Knockdown of Caprin-1 inhibited glycolytic metabolism through the down-regulation of SLC2A1, HK2, HIF1A, MYC, NUP160, NUP133, and NUP155. A Gene-set enrichment analysis (GSEA) of the interrogated glycolysis signatures between the high and low CAPRIN1 groups in the database (GSE69925); NES, normalized enrichment score. B ECAR were measured in Eca109 cells 48 h after Caprin-1 knockdown using the Seahorse XF24 extracellular flux analyzer. C Lactate production was assessed in Eca109 cells after 48 h of siRNA transfection. D Correlation between CAPRIN1 and the top four leading-edge genes contributing to GSEA in the TCGA database. E Correlation between CAPRIN1 and glycolysis genes (HIF1A and MYC) in the TCGA database. F The protein expression level of HIF1α, c-Myc, and Caprin-1 in 7 paired ESCC tissues was examined using Western blot. T, tumor; N, adjacent normal tissues. G The expression patterns of HIF1α and c-Myc in Eca109 cells after silencing Caprin-1. H Changes in mRNA levels were detected by real time PCR after the Caprin-1 1# knockdown of Eca109 cells

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