Chemokine CXCL12 activates dual CXCR4 and CXCR7-mediated signaling pathways in pancreatic cancer cells
© Heinrich et al; licensee BioMed Central Ltd. 2012
Received: 7 December 2011
Accepted: 2 April 2012
Published: 2 April 2012
Previously assumed to be a select ligand for chemokine receptor CXCR4, chemokine CXCL12 is now known to activate both CXCR4 and CXCR7. However, very little is known about the co-expression of these receptors in cancer cells.
We used immunohistochemistry to determine the extent of co-expression in pancreatic cancer tissue samples and immunoblotting to verify expression in pancreatic cancer cell lines. In cell culture studies, siRNA was used to knock down expression of CXCR4, CXCR7, K-Ras and β-arrestin -2 prior to stimulating the cells with CXCL12. Activation of the mitogen-activated protein kinase pathway (MAPK) was assessed using both a Raf-pull down assay and western blotting. The involvement of the receptors in CXCL12-mediated increases in cell proliferation was examined via an ATP-based proliferation assay.
First, we discovered frequent CXCR4/CXCR7 co-expression in human pancreatic cancer tissues and cell lines. Next, we observed consistent increases in ERK1/2 phosphorylation after exposure to CXCL12 or CXCL11, a CXCR7 agonist, in pancreatic cancer cell lines co-expressing CXCR4/CXCR7. To better characterize the receptor-mediated pathway(s), we knocked down CXCR4 or CXCR7, exposed the cells to CXCL12 and examined subsequent effects on ERK1/2. We observed that CXCR7 mediates the CXCL12-driven increase in ERK1/2 phosphorylation. Knockdown of CXCR4 expression however, decreased levels of K-Ras activity. Conversely, KRAS knockdown greatly reduced CXCL12-mediated increases in ERK1/2 phosphorylation. We then evaluated the role of β-arrestin-2, a protein directly recruited by chemokine receptors. We observed that β-arrestin-2 knockdown also inhibited increases in ERK1/2 phosphorylation mediated by both CXCR4 and CXCR7. Finally, we investigated the mechanism for CXCL12-enhanced cell proliferation and found that either receptor can modulate cell proliferation.
In summary, our data demonstrate that CXCR4 and CXCR7 are frequently co-expressed in human pancreatic cancer tissues and cell lines. We show that β-arrestin-2 and K-Ras dependent pathways coordinate the transduction of CXCL12 signals. Our results suggest that the development of therapies based on inhibiting CXCL12 signaling to halt the growth of pancreatic cancer should be focused at the ligand level in order to account for the contributions of both receptors to this signaling pathway.
It is well-established that chemokines interact with G protein-coupled receptors (GPCRs) to activate downstream signaling pathways that enhance cancer cell growth, migratory behavior, and cell survival [1, 2]. Previous studies have characterized the effects of chemokine CXCL12 in many cancers [3–5] including its role in promoting local invasion and distant metastasis of pancreatic cancer [4, 6–8]. Its corresponding receptor CXCR4 has been widely investigated initially because reports showed it is a co-receptor for T-tropic HIV-1 and HIV-2 entry into CD4+ cells [9, 10]. Since then, CXCL12 was found to be the specific ligand for CXCR4 [11, 12]. As such, the CXCL12-CXCR4 axis has been the focus of research into therapeutic strategies for pancreatic and other cancers [7, 13–15]. Recent data, however, shows that CXCL12 also binds to and activates chemokine receptor CXCR7 [16–19]. Therefore, downstream cell functions, which have been previously attributed to CXCR4, may also result from CXCR7-mediated signaling.
CXCR7 is expressed in many different tissues, including neurons, immune cells, and endothelial cells; receptor-mediated signaling can occur by binding one of its two known ligands, CXCL11 or CXCL12 [17, 18, 20, 21]. It has a dedicated role in fetal cardiac development and B-cell localization as elucidated in CXCR7-deficient mice [17, 18, 20, 21]. As with many other chemokine receptors, CXCR7 is known to induce oncogenic phenotypes apart from its innate role in organogenesis and immunity [17, 18, 20, 21]. Similar to what is known about CXCR4, recent reports have indicated that CXCR7 promotes cancer cell survival through anti-apoptotic mechanisms [17, 22]. However, in contrast to the downstream effects of CXCR4, chemotaxis has not been reported to be induced by CXCR7-mediated signaling . Although these data may suggest divergent functions for CXCR4 and CXCR7 in cancer cells, little is known regarding the frequency of co-expression and therein the mechanism for propagation of CXCL12 signals.
We previously investigated CXCL12 signaling in pancreatic cancer cells and observed enhanced cell proliferation mediated by the MAPK pathway [23, 24]. Here, our objective was to investigate the roles of CXCR4 and CXCR7 in CXCL12-driven activation of the MAPK pathway in human pancreatic cancer cells. We examined β-arrestins which are recruited by GPCRs , as well as K-Ras, which is known to regulate the MAPK pathway . Our results demonstrate that CXCR4 and CXCR7 are co-expressed with high frequency in human pancreatic cancers and that either receptor can regulate the MAPK pathway. Our results suggest that both CXCR4 and CXCR7 are potential targets in the development of effective therapies to halt the growth of pancreatic cancer.
Materials and methods
Cell culture and reagents
Human pancreatic cancer cell lines AsPC-1, MiaPaCa-2, PANC-1, SU.86.86, HS-766 T and BxPC-3,were obtained from American Type Culture Collection (ATCC; Manassas, VA) within the past 5 years. FG cells, which are a metastatic derivative of the pancreatic adenocarcinoma cells COLO-357, were provided by Dr. A. Lowy. All cells used for the experiments presented in this study were immediately cryopreserved in liquid nitrogen after they were obtained. All cell lines were assessed by DNA extraction, polymerase chain reaction (PCR) amplification, and sequencing for KRAS and TP53 gene mutations to verify the genotype of cells (data not shown). Cells were maintained in ATCC-recommended media at 37°C and 5% CO2. Serum-starvation lasted for 12-24 h unless otherwise noted.
CXCL12 and epidermal growth factor (EGF) were purchased from Peprotech (Rocky Hill, NJ); CXCL11and CXCL10 from R&D Systems (Minneapolis, MN). The following antibodies were used: rabbit polyclonal antibodies against phospho-ERK1/2 and total ERK1/2 (Cell Signaling; Beverly, MA), rabbit polyclonal antibodies against CXCR4 (Abcam, Cambridge, MA) and CXCR7 (Abcam and R&D Systems), a goat polyclonal antibody against β-arrestin-2 (Abcam), and a mouse monoclonal antibody against K-Ras (Calbiochem; San Diego, CA).
Cell lysates were collected as previously described . Twenty micrograms of protein were separated on 12% SDS-polyacrylamide gels and transfered onto PVDF membranes (Millipore; Bedford, MA). The membranes were blocked for 1 h and probed overnight with primary antibodies. After washing, membranes were labeled with horseradish peroxidase (HRP)-conjugated secondary antibodies (BioRad; Hercules, CA). Blots were developed with a chemiluminescence substrate (Amersham Pharmacia; Piscataway, NJ) and imaged.
We assessed CXCR4 and CXCR7 expression in formalin-fixed paraffin embedded (FFPE) specimens as previously described . Pancreatic cancers were obtained from patients who had undergone resection for pancreatic adenocarcinoma with Institutional Review Board approval. Tissue blocks were sectioned (5 μm) and deparaffinized with xylene. After antigen retrieval was performed, a section was incubated with the anti-CXCR4 antibody and the next consecutive section from the tissue block was incubated with the anti-CXCR7 antibody. Then, they were labeled with secondary antibody (EnVision Plus; Dako, Carpinteria, CA), developed, and examined under microscopy at 200× magnification.
Short interfering RNA (SiRNA)
Pancreatic cancer cells were transfected with siRNA (100 nM) using RNAiMAX (Invitrogen; Carlsbad, CA) according to the manufacturer's instructions and incubated for 48 h prior to application of treatments. siRNAs used were control, CXCR4, CXCR7, β-arrestin-2 and KRAS (Dharmacon; Lafayette, CO).
K-Ras activity was measured by a Raf pull-down assay (Millipore). In this enzyme-linked immunosorbent assay, cells maintained in serum-free media, were exposed to CXCL12 (100 ng/ml) or CXCL11 (200 ng/ml) for 15 min and then lysed. The cell lysate (100 μg) was incubated with Raf-1 Ras Binding Domain (RBD)-agarose. K-Ras proteins captured by Raf-1-RBD were detected and measured by the addition of an anti-K-Ras antibody (Millipore). An HRP-conjugated secondary antibody was then added. After adding a chemiluminescent substrate, signals were measured by a luminometer (Perkin-Elmer; Shelton, CT). Baseline K-Ras activity prior to stimulation with CXCL12 and CXCL11 was placed at zero; the data presented represents the relative increases in K-Ras activity due to stimulation. At least three independent assays were performed for each cell line. The mean absorbance ± one SD was plotted for each treatment group.
Cell proliferation was assessed using a proliferation assay (CellTiter-Glo, Promega; Madison, WI) based on the quantification of ATP as previously described . Cells were plated in 96-well plates at a density of 5 × 103 cells per well and exposed to CXCL12 in serum-free media for 72 h. Plates were incubated with CellTiter-Glo reagent and luminescence was measured. At least three independent cell proliferation assays were performed. Baseline proliferation prior to stimulation with CXCL12 and CXCL11 was placed at zero and the results show the relative increases due to stimulation. The mean absorbance ± one SD was plotted for each treatment group.
Statistical analysis of the data was performed using unpaired Student's t-test. P values were two-sided and values of < 0.05 were considered statistically significant.
CXCR4 and CXCR7 are co-expressed in human pancreatic cancers
CXCR4 and CXCR7 mediate the activation of the MAPK pathway
To further elucidate which receptor(s) mediate CXCL12-driven ERK phosphorylation, we performed CXCR4 or CXCR7 knockdown in PANC-1 cells (Figure 3b). Following knockdown, cells were exposed to CXCL12 (Figure 3c). CXCR7 knockdown was required to attenuate CXCL12-driven ERK phosphorylation. Our results suggest that CXCR7-mediated signaling can regulate CXCL12-driven ERK phosphorylation in cells co-expressing CXCR4/CXCR7.
CXCR4 signaling is required for the CXCL12-induced increase in K-ras activity
Signaling through CXCR4 and CXCR7 is β-arrestin-2-dependent
CXCL12 drives an increase in cell proliferation
Chemokines regulate the chemotactic responses of cells that are essential for organogenesis and immunity through the orchestration of cell movement from one location to another [30–32]. Cancer cells have misappropriated these regulatory mechanisms to stimulate their own growth, invasion, and metastasis. Numerous studies now implicate chemokines and their corresponding receptors in the invasive phenotype of many cancers . In particular, the CXCL12-CXCR4 axis has been well studied in gastrointestinal malignancies, but recent reports suggest that downstream effects once attributed to CXCR4 may also be secondary to CXCR7, an alternate or second receptor for CXCL12. As a result, we examined CXCL12 activity in pancreatic cancer cell lines. Among several novel discoveries in this investigation, we identified high frequency of CXCR4/CXCR7 co-expression in human pancreatic cancer tissues and cell lines.
Chemokine CXCL12 is characteristically expressed in select tissues, but may also be expressed via an autocrine feedback loop mechanism in pancreatic cancer cells . As such, the resources for CXCL12 to activate CXCR4 or CXCR7-mediated signaling pathways are present. Since our studies target pancreatic cancer, we examined CXCL12 signaling within the framework of K-Ras. The gene encoding KRAS is frequently mutated in patients with pancreatic cancer which results in a gain-of-function that may contribute to the pathogenesis and progression of this cancer [33, 34]. Here, we made several novel observations regarding K-Ras. First, we discovered that pancreatic cancer cells co-expressing CXCR4/CXCR7 had increased levels of ERK phosphorylation and K-Ras activity when exposed to CXCL12. These CXCL12-induced increases in K-Ras activity were not observed with other ligands in endometrial and pancreatic cancer cells harboring mutant KRAS [35, 36]. Our results, therefore, suggest that CXCL12 may hyperactivate K-Ras activity levels even though there is baseline mutant-derived K-Ras activity. Second, we observed that CXCR4, rather than CXCR7, was the receptor that regulated this response. GPCRs can signal through a canonical or non-canonical pathway, activation of K-Ras may occur via a CXCR4-mediated activation of the canonical GPCR pathway [37–39]. CXCR7 on the other hand signals through the non-canonical pathway , which explains why CXCR7 activation leads to ERK1/2 phosphorylation but not an increase in K-Ras activity. Β-arrestin-2 is an important member of either pathway, and we show that its knockdown blocks ERK phosphorylation. Third, we determined that CXCL12-driven increases in cancer cell proliferation can occur through either receptor and hence either signaling pathway.
Specific inhibitors to CXCR4 and CXCR7 are currently unavailable for clinical use. AMD3100 was believed to selectively bind and antagonize CXCR4 activity [40, 41]. Derivatives of AMD3100 are also under investigation for their effects on cancer cells . A recent study has demonstrated that AMD3100 specifically binds to and activates CXCR7 . Therefore, in contrast to its antagonism of the CXCL12-CXCR4 interaction, AMD3100 positively modulates CXCL12 effects and binding to CXCR7.
In summary, we report that CXCR4 and CXCR7 are co-expressed with high frequency in human pancreatic cancer specimens and cell lines. CXCR4 and CXCR7 signaling is β-arrestin-2-dependent and controls CXCL12 signals to the MAPK pathway. CXCL12 activates both canonical and non-canonical GPCR pathways in pancreatic cancer cell lines. This has functional significance in that we show signaling through either pathway leads to an increase in cell proliferation upon exposure to CXCL12. Hence our study suggests that efforts to therapeutically target CXCL12 signaling in pancreatic cancer should be focused at the level of the ligand to account for both CXCR4 and CXCR7 activity.
We appreciate the laboratory work of Xiaoming Shen, Ph.D. This work utilized City of Hope's Pathology Core, which is supported in part by Cancer Center Support Grant NCI P30 CA33572. This study was also supported by NIH CA134637 (JK) and American Cancer Society RSG-11-070-01-TBE (JK). The contents are solely the responsibility of the authors and do not represent the official views of the NIH or ACS.
- Balkwill F: Cancer and the chemokine network. Nat Rev Cancer. 2004, 4: 540-550. 10.1038/nrc1388.View ArticlePubMedGoogle Scholar
- Ratajczak M, Zuba-Surma E, Kucia M, Reca R, Wojakowski W, Ratajczak J: The pleiotropic effects of the SDF-1-CXCR4 axis in organogenesis, regeneration and tumorigenesis. Leukemia. 2006, 20: 1915-1924. 10.1038/sj.leu.2404357.View ArticlePubMedGoogle Scholar
- Corcione A, Ottonello L, Tortolina G, Facchetti P, Airoldi I, Guglielmino R, Dadati P, Truini M, Sozzani S, Dallegri F, Pistola V: Stromal cell-derived factor-1 as a chemoattractant for follicular center lymphoma B cells. J Natl Cancer Inst. 2000, 92: 628-635. 10.1093/jnci/92.8.628.View ArticlePubMedGoogle Scholar
- Koshiba T, Hosotani R, Miyamoto Y, Ida J, Tsuji S, Nakajima S, Kawaguchi M, Kobayashi H, Doi R, Hori T: Expression of stromal cell-derived factor 1 and CXCR4 ligand receptor system in pancreatic cancer: a possible role for tumor progression. Clin Cancer Res. 2000, 6: 3530-3535.PubMedGoogle Scholar
- Scotton C, Wilson J, Scott K, Stamp G, Wilbanks G, Fricker S, Bridger G, Balkwill F: Multiple actions of the chemokine CXCL12 on epithelial tumor cells in human ovarian cancer. Cancer Res. 2002, 62: 5930-5938.PubMedGoogle Scholar
- Saur D, Seidler B, Schneider G, Algül H, Beck R, Senekowitsch-Schmidtke R, Schwaiger M, Schmid R: CXCR4 expression increases liver and lung metastasis in a mouse model of pancreatic cancer. Gastroenterology. 2005, 129: 1237-1250. 10.1053/j.gastro.2005.06.056.View ArticlePubMedGoogle Scholar
- Marchesi F, Monti P, Leone B, Zerbi A, Vecchi A, Piemonti L, Mantovani A, Allavena P: Increased survival, proliferation, and migration in metastatic human pancreatic tumor cells expressing functional CXCR4. Cancer Res. 2004, 64: 8420-8427. 10.1158/0008-5472.CAN-04-1343.View ArticlePubMedGoogle Scholar
- Mori T, Doi R, Koizumi M, Toyoda E, Ito D, Kami K, Masui T, Fujimoto K, Tamamura H, Hiramatsu K: CXCR4 antagonist inhibits stromal cell-derived factor 1-induced migration and invasion of human pancreatic cancer. Mol Cancer Ther. 2004, 3: 29-37. 10.4161/cbt.3.1.547.PubMedGoogle Scholar
- Feng Y, Broder C, Kennedy P, Berger E: HIV-1 entry cofactor: functional cDNA cloning of a seven-transmembrane, G protein-coupled receptor. Science. 1996, 272: 872-877. 10.1126/science.272.5263.872.View ArticlePubMedGoogle Scholar
- Endres M, Clapham P, Marsh M, Ahuja M, Turner J, McKnight A, Thomas J, Stoebenau-Haggarty B, Choe S, Vance P: CD4-independent infection by HIV-2 is mediated by fusin/CXCR4. Cell. 1996, 87: 745-756. 10.1016/S0092-8674(00)81393-8.View ArticlePubMedGoogle Scholar
- Bleul C, Farzan M, Choe H, Parolin C, Clark-Lewis I, Sodroski J, Springer T: The lymphocyte chemoattractant SDF-1 is a ligand for LESTR/fusin and blocks HIV-1 entry. Nature. 1996, 382: 829-833. 10.1038/382829a0.View ArticlePubMedGoogle Scholar
- Oberlin E, Amara A, Bachelerie F, Bessia C, Virelizier J, Arenzana-Seisdedos F, Schwartz O, Heard J, Clark-Lewis I, Legler D: The CXC chemokine SDF-1 is the ligand for LESTR/fusin and prevents infection by T-cell-line-adapted HIV-1. Nature. 1996, 385: 833-835.View ArticleGoogle Scholar
- Rubin J, Kung A, Klein R, Chan J, Sun Y, Schmidt K, Kieran M, Luster A, S RA: A small-molecule antagonist of CXCR4 inhibits intracranial growth of primary brain tumors. Proc Natl Acad Sci USA. 2003, 100: 13513-13518. 10.1073/pnas.2235846100.View ArticlePubMedPubMed CentralGoogle Scholar
- Zeelenberg I, Ruuls-Van Stalle L, Roos E: The chemokine receptor CXCR4 is required for outgrowth of colon carcinoma micrometastases. Cancer Res. 2003, 63: 3833-3839.PubMedGoogle Scholar
- Müller A, Homey B, Soto H, Ge N, Catron D, Buchanan M, McClanahan T, Murphy E, Yuan W, Wagner S: Involvement of chemokine receptors in breast cancer metastasis. Nature. 2001, 410: 50-56. 10.1038/35065016.View ArticlePubMedGoogle Scholar
- Balabanian K, Lagane B, Infantino S, Chow K, Harriague J, Moepps B, Arenzana-Seisdedos F, Thelen M, Bachelerie F: The chemokine SDF-1/CXCL12 binds to and signals through the orphan receptor RDC1 in T lymphocytes. J Biol Chem. 2005, 280: 35760-35766. 10.1074/jbc.M508234200.View ArticlePubMedGoogle Scholar
- Burns J, Summers B, Wang Y, Melikian A, Berahovich R, Miao Z, Penfold M, Sunshine M, Littman D, Kuo C: A novel chemokine receptor for SDF-1 and I-TAC involved in cell survival, cell adhesion, and tumor development. J Exp Med. 2006, 203: 2201-2213. 10.1084/jem.20052144.View ArticlePubMedPubMed CentralGoogle Scholar
- Miao Z, Luker K, Summers B, Berahovich R, Bhojani M, Rehemtulla A, Kleer C, Essner J, Nasevicius A, Luker G: CXCR7 (RDC1) promotes breast and lung tumor growth in vivo and is expressed on tumor-associated vasculature. Proc Natl Acad Sci USA. 2007, 104: 15735-15740. 10.1073/pnas.0610444104.View ArticlePubMedPubMed CentralGoogle Scholar
- Sierro F, Biben C, Martinez-Munoz L, Mellado M, Ransohoff R, Li M, Woehl B, Leung H, Groom J, Batten M: Disrupted cardiac development but normal hematopoiesis in mice deficient in the second CXCL12/SDF-1 receptor, CXCR7. Proc Natl Acad Sci USA. 2007, 104: 14759-14764. 10.1073/pnas.0702229104.View ArticlePubMedPubMed CentralGoogle Scholar
- Wang Y, Li G, Stanco A, Long J, Crawford D, Potter G, Pleasure S, Behrens T: JL R: CXCR4 and CXCR7 have distinct functions in regulating interneuron migration. Neuron. 2011, 69: 61-76. 10.1016/j.neuron.2010.12.005.View ArticlePubMedPubMed CentralGoogle Scholar
- Infantino S, Moepps B, Thelen M: Expression and regulation of the orphan receptor RDC1 and its putative ligand in human dendritic and B cells. J Immunol. 2006, 176: 2197-2207.View ArticlePubMedGoogle Scholar
- Hattermann K, Held-Feindt J, Lucius R, Müerköster S, Penfold M, Schall T, Mentlein R: The chemokine receptor CXCR7 is highly expressed in human glioma cells and mediates antiapoptotic effects. Cancer Res. 2010, 70: 3299-3308. 10.1158/0008-5472.CAN-09-3642.View ArticlePubMedGoogle Scholar
- Shen X, Jackson D, Artinyan A, Thomas R, Lowy A, Kim J: Chemokine receptor CXCR4 enhances proliferation in pancreatic cancer cells through AKT and ERK dependent pathways. Pancreas. 2010, 39: 81-87. 10.1097/MPA.0b013e3181bb2ab7.View ArticlePubMedGoogle Scholar
- Thomas R, Kim J, Revelo-Penafiel M, Angel R, Lowy A: The chemokine receptor CXCR4 is expressed in pancreatic intraepithelial neoplasia. Gut. 2008, 57: 1555-1560. 10.1136/gut.2007.143941.View ArticlePubMedGoogle Scholar
- Rajagopal S, Rajagopal K, Lefkowitz R: Teaching old receptors new tricks: biasing seven-transmembrane receptors. Nat Rev Drug Discov. 2010, 9: 373-386. 10.1038/nrd3024.View ArticlePubMedPubMed CentralGoogle Scholar
- Pohl G, Ho C, Kurman R, Bristow R, Wang T, Shih I: Inactivation of the mitogen-activated protein kinase pathway as a potential target-based therapy in ovarian serous tumors with KRAS or BRAF mutations. Cancer Res. 2005, 65: 1994-2000. 10.1158/0008-5472.CAN-04-3625.View ArticlePubMedGoogle Scholar
- Shen X, Mailey B, Ellenhorn J, Chu P, Lowy A, Kim J: CC chemokine receptor 9 enhances proliferation in pancreatic intraepithelial neoplasia and pancreatic cancer cells. J Gastrointest Surg. 2009, 13: 1955-1962. 10.1007/s11605-009-1002-8.View ArticlePubMedGoogle Scholar
- Xu H, Wu Q, Dang S, Jin M, Xu J, Cheng Y, Pan M, Wu Y, Zhang C, Zhang Y: Alteration of CXCR7 expression mediated by TLR4 promotes tumor cell proliferation and migration in human colorectal carcinoma. PLoS One. 2011, 6: e27399-10.1371/journal.pone.0027399.View ArticlePubMedPubMed CentralGoogle Scholar
- Weinstein I, Joe A: Oncogene addiction. Cancer Res. 2008, 68: 3077-3080.PubMedGoogle Scholar
- Rossi D, Zlotnik A: The biology of chemokines and their receptors. Annu Rev Immunol. 2000, 18: 217-242. 10.1146/annurev.immunol.18.1.217.View ArticlePubMedGoogle Scholar
- Zlotnik A, Yoshie O: Chemokines: a new classification system and their role in immunity. Immunity. 2000, 12: 121-127. 10.1016/S1074-7613(00)80165-X.View ArticlePubMedGoogle Scholar
- Balkwill F: Chemokine biology in cancer. Semin Immunol. 2003, 15: 49-55.PubMedGoogle Scholar
- Deramaudt T, Rustgi A: Mutant KRAS in the initiation of pancreatic cancer. Biochim Biophys Acta. 2005, 1756: 97-101.PubMedGoogle Scholar
- Hruban R, Wilentz R, Kern S: Genetic progression in the pancreatic ducts. Am J Pathol. 2000, 156: 1821-1825. 10.1016/S0002-9440(10)65054-7.View ArticlePubMedPubMed CentralGoogle Scholar
- Kato K, Ueoka Y, Kato K, Tamura T, Nishida J, Wake N: Oncogenic Ras modulates epidermal growth factor responsiveness in endometrial carcinomas. Eur J Cancer. 1998, 34: 737-744. 10.1016/S0959-8049(97)10124-1.View ArticlePubMedGoogle Scholar
- Watanabe M, Nobuta A, Tanaka J, Asaka M: An effect of K-ras gene mutation on epidermal growth factor receptor signal transduction in PANC-1 pancreatic carcinoma cells. Int J Cancer. 1996, 67: 264-268. 10.1002/(SICI)1097-0215(19960717)67:2<264::AID-IJC18>3.0.CO;2-B.View ArticlePubMedGoogle Scholar
- DeFea KA: Beta-arrestins as regulators of signal termination and transduction: how do they determine what to scaffold?. Cell Signal. 2011, 23: 621-629. 10.1016/j.cellsig.2010.10.004.View ArticlePubMedGoogle Scholar
- Rajagopal S, Rajagopal K, Lefkowitz RJ: Teaching old receptors new tricks: biasing seven-transmembrane receptors. Nat Rev Drug Discov. 2010, 9: 373-386. 10.1038/nrd3024.View ArticlePubMedPubMed CentralGoogle Scholar
- Rajagopal S, Kim J, Ahn S, Craig S, Lam CM, Gerard NP, Gerard C, Lefkowitz RJ: Beta-arrestin- but not G protein-mediated signaling by the "decoy" receptor CXCR7. Proc Natl Acad Sci USA. 2010, 107: 628-632. 10.1073/pnas.0912852107.View ArticlePubMedPubMed CentralGoogle Scholar
- Hatse S, Princen K, Bridger G, De Clercq E, Schols D: Chemokine receptor inhibition by AMD3100 is strictly confined to CXCR4. FEBS Lett. 2002, 527: 255-262. 10.1016/S0014-5793(02)03143-5.View ArticlePubMedGoogle Scholar
- Kalatskaya I, Berchiche Y, Gravel S, Limberg B, Rosenbaum J, Heveker N: AMD3100 is a CXCR7 ligand with allosteric agonist properties. Mol Pharmacol. 2009, 75: 1240-1247. 10.1124/mol.108.053389.View ArticlePubMedGoogle Scholar
- De Clercq E: Potential clinical applications of the CXCR4 antagonist bicyclam AMD3100. Mini Rev Med Chem. 2005, 5: 805-824. 10.2174/1389557054867075.View ArticlePubMedGoogle Scholar
This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.