Figure 3

The effect of CXCL12 exposure on MAPK signaling. a) Pancreatic cancer cells PANC-1 and MiaPaCa2 were serum-starved and then exposed to CXCL12 (100 ng/ml) and CXCL11 (200 ng/ml) over a range of time periods (5 min to 1 h). Immunoblotting was performed for phosphorylated ERK1/2. Total ERK1/2 and GAPDH were used as loading controls. b) PANC-1 cells were transfected with control, CXCR4, or CXCR7 siRNA (100 nM) using RNAiMAX (10 μl). The immunoblots verify down-regulation of CXCR4 and CXCR7 expression. c) Following transfection, the cells were serum-starved and then exposed to CXCL12 (100 ng/ml). Immunoblotting was performed for phospho-ERK1/2. Total ERK1/2 and GAPDH were used as loading controls.