Figure 6

β-arrestin-2's role in CXCR4 and CXCR7-driven ERK phosphorylation. PANC-1 cells were transfected with control or β-arrestin-2 siRNA (100 nM) using RNAiMAX (10 μl). Cells were serum-starved and then exposed to CXCL12 (100 ng/ml) or CXCL11 (200 ng/ml) for 15 min. The immunoblot shows β-arrestin-2 and phospho-ERK1/2 expression with total ERK1/2 and GAPDH serving as loading controls.