- Open Access
Clinical hypothermia temperatures increase complement activation and cell destruction via the classical pathway
© Shah et al.; licensee BioMed Central Ltd. 2014
- Received: 11 December 2013
- Accepted: 16 June 2014
- Published: 24 June 2014
Therapeutic hypothermia is a treatment modality that is increasingly used to improve clinical neurological outcomes for ischemia-reperfusion injury-mediated diseases. Antibody-initiated classical complement pathway activation has been shown to contribute to ischemia-reperfusion injury in multiple disease processes. However, how therapeutic hypothermia affects complement activation is unknown. Our goal was to measure the independent effect of temperature on complement activation, and more specifically, examine the relationship between clinical hypothermia temperatures (31–33°C), and complement activation.
Antibody-sensitized erythrocytes were used to assay complement activation at temperatures ranging from 0-41°C. Individual complement pathway components were assayed by ELISA, Western blot, and quantitative dot blot. Peptide Inhibitor of complement C1 (PIC1) was used to specifically inhibit activation of C1.
Antibody-initiated complement activation resulting in eukaryotic cell lysis was increased by 2-fold at 31°C compared with 37°C. Antibody-initiated complement activation in human serum increased as temperature decreased from 37°C until dramatically decreasing at 13°C. Quantitation of individual complement components showed significantly increased activation of C4, C3, and C5 at clinical hypothermia temperatures. In contrast, C1s activation by heat-aggregated IgG decreased at therapeutic hypothermia temperatures consistent with decreased enzymatic activity at lower temperatures. However, C1q binding to antibody-coated erythrocytes increased at lower temperatures, suggesting that increased classical complement pathway activation is mediated by increased C1 binding at therapeutic hypothermia temperatures. PIC1 inhibited hypothermia-enhanced complement-mediated cell lysis at 31°C by up to 60% (P = 0.001) in a dose dependent manner.
In summary, therapeutic hypothermia temperatures increased antibody-initiated complement activation and eukaryotic cell destruction suggesting that the benefits of therapeutic hypothermia may be mediated via other mechanisms. Antibody-initiated complement activation has been shown to contribute to ischemia-reperfusion injury in several animal models, suggesting that for diseases with this mechanism hypothermia-enhanced complement activation may partially attenuate the benefits of therapeutic hypothermia.
- Therapeutic hypothermia
- Classical pathway
- Complement inhibitor
- Ischemia-reperfusion injury
Therapeutic hypothermia improves neurological outcome in ischemia-reperfusion injury (IRI) caused by cardiac arrest , traumatic brain injury , stroke , acute liver injury  and neonatal hypoxic ischemic encephalopathy . In addition to decreased cerebral metabolism , hypothermia appears to attenuate multiple processes that contribute to tissue damage following IRI including neuroinflammation , excitotoxicty , mitochondrial dysfunction , apoptosis , free radical production , seizure activity  and blood-brain barrier disruption . Hypothermia is also purported to stimulate protective cold shock proteins .
The complement system is an extremely potent inflammatory cascade composed of more than 30 plasma and cell membrane proteins that plays major roles in innate immune defense as well as many inflammatory diseases including IRI. Clinical and experimental studies in multiple organ systems have shown that reperfusion following ischemia results in local activation of the complement system . Complement activation in IRI is primarily mediated by circulating natural antibodies (IgM) binding to neo-antigens expressed on the surface of hypoxia-stressed endothelial cells leading to classical complement pathway activation or lectin pathway activation [16, 17]. In addition to direct complement-mediated endothelial cell damage during reperfusion, complement induced neutrophil recruitment and activation causes significant injury through the phagocytic actions of macrophages, free radical production and synthesis of toxic products . Complement inhibition has been shown to be protective in animal models of brain , kidney  and liver  IRI.
While several mechanisms of action for therapeutic hypothermia have been proposed, the effect of hypothermia on complement activation is unknown. Whether improved outcomes in IRI could be due, in part, to hypothermia inhibiting or attenuating complement activation has not yet been explored. Such a mechanism is plausible given that complement activation is enzymatic and slows down dramatically at very low temperatures of 0 – 4°C [21, 22]. However, whether the temperatures of clinical hypothermia, 31 - 33°C, would inhibit complement activation remains untested. The increasing use of therapeutic hypothermia in the treatment of IRI diseases makes understanding the contribution of the complement system critical. These experiments elucidate how therapeutic hypothermia temperatures modulate antibody-initiated complement activation.
Pooled normal human serum (NHS) was derived from the blood of healthy human volunteers obtained with written informed consent in accordance with an Institutional Review Board approved protocol (IRB 02-06-EX-0216, Eastern Virginia Medical School).
Sheep erythrocytes were purchased from MP Biomedicals, LLC. Anti-sheep-erythrocyte-IgM was purchased from Rockland Incorporated. Sheep erythrocytes were incubated with antibody at 30°C to generate antibody-sensitized cells (EA), as previously described . All antibody-sensitization was performed at 30°C. Standard complement buffers were prepared for hemolytic assays: GVBS++ (Veronal buffered saline, 0.1% gelatin, 0.15 mM CaCl2, and 1.0 mM MgCl2), Mg-EGTA-GVBS (Veronal buffered saline with 5 mM MgCl2 and 8 mM EGTA), and GVBS- - (Veronal buffered saline, 0.1% gelatin, 0.01 M EDTA), Veronal Buffered Saline (10 mM Barbital, 145 mM NaCl, pH 7.4). Peptide Inhibitor of Complement C1 (PIC1) version AcPA, described elsewhere , was synthesized by New England Peptide (Gardner, MA). Lyophilized PIC1 was reconstituted in DMSO. Pooled normal human serum (NHS) was prepared as previously described . C8-depleted human serum and purified C1q were purchased from CompTech (Tyler, TX).
Antibody-initiated complement hemolytic assays were performed by incubating 100 μL EA with 0.2% NHS in GVBS++. Hemolytic assays were performed for one hour at temperatures ranging from 0°C to 37°C. A separate assay was performed with more clinically relevant temperatures of 31°, 33°, 35°, 37°, 39°, and 41°C. EA were also incubated with water or buffer as controls. Hemolysis reactions were quenched with 4 mL GVBS- - and the supernatants recovered after sedimentation. Hemolysis was quantified by spectrophotometry of the supernatants at 412 nm and normalized as previously described . Alternative complement pathway hemolytic assays were performed by incubating rabbit erythrocytes (CompTech, Tyler, TX) with 4% NHS in Mg-EGTA-GVBS at temperatures ranging from 0°C to 37°C. A separate assay was performed with more clinically relevant temperatures of 31°, 33°, 35°, 37°, 39°, and 41°C. Reactions were quenched with 2 mL GVBS- -, sedimented and measured as above.
Samples were generated by incubating 1% NHS with 400 μg/ml heat-aggregated IgG in GVBS++ at each temperature for one hour. Heat-aggregated IgG was generated from purified human IgG (Gammagard, Baxter) as described elsewhere . The reaction was stopped with EDTA-GVBS- - and the samples were measured by C5a ELISA (R&D Systems). Background values from controls of NHS and heat-aggregated human IgG incubated on ice for one hour were subtracted.
C3 and C4 assays
C3/C4 samples were generated by incubating C8-deficient serum (to prevent hemolysis), 1% final, with 0.25 mL EA in a total of 0.75 mL of GVBS++ for one hour at each temperature. After incubation, the samples were washed twice with water to remove hemoglobin, and the resulting membrane bound C3/C4 was stripped using 25 mM methylamine. These samples were quantitated by total C3 ELISA, iC3b ELISA, and C4 ELISA as previously described . C3 and C4 Western blot analysis was performed as described elsewhere .
C1s western blot
A Western blot to assess C1s activation by heat aggregated IgG has been previously described [27, 28] One microliter of partially purified C1 (0.2 mg/ml, Complement Technologies, Inc.), 5 μl of heat-aggregated IgG (1:250 dilution from 50 μg/ml stock), and 5 μl of PBS were combined on ice and samples incubated at the following temperatures: 31, 33, 35, 37, 39 and 41°C for 0, 30, 45, 60 and 90 minutes. After incubation, 4 μl of loading buffer was added and each sample was boiled. Gel electrophoresis on a 10% SDS-PAGE gel was performed at 140 V for 60 minutes. The gel was then transferred to a nitrocellulose membrane at 200 mA for 60 minutes. The membrane was washed twice for five minutes in PBS buffer and then blocked overnight in 5% NFDM-PBS. The next day, the membrane was probed with primary antibody, Goat anti-C1s (Quidel), at a dilution of 1:2000 in 5% NFDM-PBS/0.1% Tween-20 for one hour at room temperature. The membrane was washed 3X for 10 minutes with PBS/0.1% Tween-20. Then, the membrane was probed with secondary antibody, Donkey anti-goat IR680 (Li-cor Biosciences), at a dilution of 1:10,000 in 5% NFDM-PBS/0.1% Tween-20 for one hour at room temperature. After washing the membrane 3X with PBS/0.1% Tween-20, it was imaged on an Odyssey imager to determine the amounts of the C1s heavy and light chains characteristic of activated C1s relative to the proenzyme species.
C1 either from NHS (0.33% final) or C8-deficient serum (CompTech), or purified C1q (Comp Tech) at 0.24 μg/ml final, was combined with 100 μl of sensitized sheep erythrocytes in a total volume of 0.75 ml of GVBS++ for one hour at the various temperatures. These cells were then washed twice with water and the resulting cell pellet was dissolved with 0.5% NP40. Membrane bound C1q was analyzed by a quantitative Dot Blot assay. Pure C1q was titrated onto PVDF membrane, along with the samples, via a Dot Blot apparatus. The membrane was blocked with 3% BSA, probed with a goat anti-C1q antibody (Comp Tech), and then an HRP-labeled rabbit anti-goat secondary antibody (Sigma-Aldrich). Bound antibody was detected by enhanced chemiluminescence. Using the Quantity One software (Bio-Rad), grey scale values were assigned to the pure C1q titration and the samples so that a linear regression could be used to quantify the amount of C1q in each sample.
PIC1 inhibition of cold-enhanced complement activation
Peptide Inhibitors of complement C1 (PIC1) specifically inhibit C1 activation, as previously described [24, 28]. Here we use the AcPA form (IALILEPICCQERAA)  of PIC1. Preparations of 16.5% normal human serum (NHS) in GVBS++ were incubated with 0.98 mM, 0.82 mM, 0.65 mM, 0.48 mM or 0.32 mM PIC1, DMSO vehicle control, or buffer control at 4°C for 1 hour. Samples were further diluted 1:10 in GVBS++. Hemolysis reactions were then performed with 100 μL of NHS sample plus 550 μL of additional GVBS++ and 100 μL of EA over 1 hour at either in 31° or 37°C. Controls and samples were processed and measured by spectrophotometry at 412 nm.
Means and standard error of the means (SEM) were calculated from independent experiments. Statistical comparisons were made using one way ANOVA and Student’s t-test where appropriate. Statistical analysis was performed using GraphPad InStat 3 software.
Functional hemolytic assays of complement activation at hypothermic temperatures
Complement cascade activation assays
C3 is the central component of the complement cascades and its activation results in generation of the anaphylatoxin C3a and opsonization of targets with the C3-fragments, C3b and iC3b. We tested C3 activation and opsonization with C3b/iC3b by incubating EA with C8-deficient serum (to prevent hemolysis). Though total C3-fragment opsonization as a function of temperature was not statistically significant (ANOVA P = 0.5) (Figure 2B), cell membrane bound iC3b differed significantly as a function of temperature (ANOVA P < 0.01), with a 2-fold increase at 31°C versus 37°C (t-test P = 0.04) (Figure 2C). Western blot analysis of membrane-bound C3-fragments confirmed that iC3b was the predominant form and suggested increased iC3b deposition at lower temperatures (Figure 2D) consistent with ELISA data. These data show that C3 activation and iC3b opsonization increased at clinical hypothermia temperatures, suggesting that these temperatures could increase opsonization of host cells (e.g. endothelial cells) during reperfusion targeting them for phagocyte attack.
In order to specifically evaluate the classical complement pathway, we tested C4 activation by incubating EA with C8-deficient serum. C4-fragment binding differed significantly as a function of temperature (ANOVA P = 0.04). There was an almost 2-fold increase in C4 generation at 31°C compared with 37°C (t-test P = 0.05) (Figure 2E). Western blot analysis demonstrated a mixture of C4b, iC4b and C4d fragments (Figure 2F) and suggested increased C4-fragment binding at 31°C. These findings show that classical pathway activation is increased at therapeutic hypothermia temperatures and suggests that C1 activation, which cleaves C4, is also increased at these temperatures.
C1 activation and binding
Inhibition of C1-mediated complement activation
Our data show that increased complement activation at lower temperatures was mediated by antibody-initiated complement activation, but the alternative pathway was unaffected by the temperatures tested. Therapeutic hypothermia temperatures increased complement-mediated cell lysis demonstrating enhanced membrane attack complex pore formation. At lower temperatures there was increased C5a generation, which recruits and activates neutrophils enhancing local inflammation. Increased iC3b opsonization of eukaryotic cells occurred at lower temperatures, which targets them for attack by phagocytic cells. Increased C4 activation at low temperatures suggested increased activation at C1, which cleaves C4. Testing activation of the enzymatic components of C1 showed decreased enzymatic activity at lower temperatures, which is a common property of enzymatic reactions. However, multiple assays testing C1 or C1q binding showed a consistent trend toward increased binding to antibody-sensitized cells at lower temperatures. Increased binding of molecules often occurs at lower temperatures  and suggested that increased binding of C1 may be responsible for increased antibody-initiated complement activation despite decreased enzymatic activity. Increased antibody-initiated complement activation was not mediated by temperature effects on antibody-binding, because all antibody-sensitization procedures were performed at 30°C. Using serum from a patient with cutaneous vasculitis, it was found that cryoglobulin increased activation of the complement system at 20°C .
It is notable that antibody-initiated complement activation was decreased at 41°C compared with 37°C. We speculate that decreased antibody-initiated complement activation at febrile temperatures may be a mechanism of dampening classical pathway activation after systemic inflammation with cytokines and TNF has been achieved. Classical pathway activation occurs extremely early in inflammation and once initiated, complement activation will be perpetuated by the positive-feedback loop of the alternative pathway, which does not appear to be affected by febrile temperatures. Thus, this may be a down-regulatory mechanism to decrease further activation of the classical pathway and moderating potential complement-mediated damage to the host. Although mechanistically different, fever decreasing classical pathway activation may be functionally similar to soluble TNF-receptor generation down-regulating TNF effects late in inflammation [36, 37].In order to elucidate the mechanism of hypothermia-enhanced antibody-initiated complement activation, we tested a specific inhibitor of C1 activation (Figure 6). PIC1 successfully inhibited hypothermia-enhanced complement activation at 31°C to a level similar to that which occurred at euthermia (37°C), consistent with C1-mediated activation.
Our findings differ from a study demonstrating attenuated complement activation following hypothermia in vivo in a cohort of cardiopulmonary bypass patients . However, the findings in the study cannot be attributed to hypothermia alone, since the practices of hemodilution and heparinization in cardiopulmonary bypass utilized in this study likely confound the association as they are independent causes of complement inhibition . One of the limitations of this study is that it does not model IRI. However, this limitation allowed us to explore the independent effect of hypothermia on antibody-initiated complement activation. Future directions include testing the effects of hypothermia in the Vannucci model of rat brain IRI  and elucidating the contribution of the lectin and classical pathway in hypothermia enhanced complement activation.
Therapeutic hypothermia temperatures increased antibody-initiated complement activation and eukaryotic cell destruction suggesting that the benefits of therapeutic hypothermia may be mediated via other mechanisms. A peptide inhibitor of complement (PIC1) significantly inhibited this enhanced complement activation. Antibody-initiated complement activation has been shown to contribute to ischemia-reperfusion injury in several animal models, suggesting that for diseases with this mechanism hypothermia-enhanced complement activation may partially attenuate the benefits of therapeutic hypothermia. Future directions include testing the relationship between complement activation and hypothermia in an animal model of neonatal hypoxic-ischemic encephalopathy.
This work was supported by a grant from NIH (R21AI095750) awarded to Dr. Krishna.
- Group HCAS: Mild therapeutic hypothermia to improve the neurologic outcome after cardiac arrest. N Engl J Med. 2002, 346: 549-556.View ArticleGoogle Scholar
- BT Foundation, Surgeons AAoN, Surgeons CoN: Guidelines for the management of severe traumatic brain injury. J Neurotrauma. 2007, 24 (Suppl 1): S1-106.Google Scholar
- Yenari MA, Hemmen TM: Therapeutic hypothermia for brain ischemia: where have we come and where do we go?. Stroke. 2010, 41: S72-74. 10.1161/STROKEAHA.110.595371.PubMed CentralView ArticlePubMedGoogle Scholar
- Stravitz RT, Larsen FS: Therapeutic hypothermia for acute liver failure. Crit Care Med. 2009, 37: S258-264. 10.1097/CCM.0b013e3181aa5fb8.View ArticlePubMedGoogle Scholar
- Shankaran S, Laptook AR, Ehrenkranz RA, Tyson JE, McDonald SA, Donovan EF, Fanaroff AA, Poole WK, Wright LL, Higgins RD, Finer NN, Carlo WA, Duara S, Oh W, Cotten CM, Stevenson DK, Stoll BJ, Lemons JA, Guillet R, Jobe AH: Whole-body hypothermia for neonates with hypoxic-ischemic encephalopathy. N Engl J Med. 2005, 353: 1574-1584. 10.1056/NEJMcps050929.View ArticlePubMedGoogle Scholar
- Erecinska M, Thoresen M, Silver IA: Effects of hypothermia on energy metabolism in Mammalian central nervous system. J Cereb Blood Flow Metab. 2003, 23: 513-530.View ArticlePubMedGoogle Scholar
- Kimura A, Sakurada S, Ohkuni H, Todome Y, Kurata K: Moderate hypothermia delays proinflammatory cytokine production of human peripheral blood mononuclear cells. Crit Care Med. 2002, 30: 1499-1502. 10.1097/00003246-200207000-00017.View ArticlePubMedGoogle Scholar
- Polderman KH: Mechanisms of action, physiological effects, and complications of hypothermia. Crit Care Med. 2009, 37: S186-202. 10.1097/CCM.0b013e3181aa5241.View ArticlePubMedGoogle Scholar
- Ning XH, Chen SH, Xu CS, Li L, Yao LY, Qian K, Krueger JJ, Hyyti OM, Portman MA: Hypothermic protection of the ischemic heart via alterations in apoptotic pathways as assessed by gene array analysis. J Appl Physiol. 2002, 92: 2200-2207.View ArticlePubMedGoogle Scholar
- Xu L, Yenari MA, Steinberg GK, Giffard RG: Mild hypothermia reduces apoptosis of mouse neurons in vitro early in the cascade. J Cereb Blood Flow Metab. 2002, 22: 21-28.View ArticlePubMedGoogle Scholar
- Globus MY, Busto R, Lin B, Schnippering H, Ginsberg MD: Detection of free radical activity during transient global ischemia and recirculation: effects of intraischemic brain temperature modulation. J Neurochem. 1995, 65: 1250-1256.View ArticlePubMedGoogle Scholar
- Karkar KM, Garcia PA, Bateman LM, Smyth MD, Barbaro NM, Berger M: Focal cooling suppresses spontaneous epileptiform activity without changing the cortical motor threshold. Epilepsia. 2002, 43: 932-935. 10.1046/j.1528-1157.2002.03902.x.View ArticlePubMedGoogle Scholar
- Huang ZG, Xue D, Preston E, Karbalai H, Buchan AM: Biphasic opening of the blood–brain barrier following transient focal ischemia: effects of hypothermia. Can J Neurol Sci. 1999, 26: 298-304.View ArticlePubMedGoogle Scholar
- Schaller B, Graf R: Hypothermia and stroke: the pathophysiological background. Pathophysiology. 2003, 10: 7-35. 10.1016/j.pathophys.2003.09.001.View ArticlePubMedGoogle Scholar
- Mastellos D, Morikis D, Isaacs SN, Holland MC, Strey CW, Lambris JD: Complement: structure, functions, evolution, and viral molecular mimicry. Immunol Res. 2003, 27: 367-386. 10.1385/IR:27:2-3:367.View ArticlePubMedGoogle Scholar
- Elvington A, Atkinson C, Kulik L, Zhu H, Yu J, Kindy MS, Holers VM, Tomlinson S: Pathogenic natural antibodies propagate cerebral injury following ischemic stroke in mice. J Immunol. 2012, 188: 1460-1468. 10.4049/jimmunol.1102132.PubMed CentralView ArticlePubMedGoogle Scholar
- Chan RK, Ibrahim SI, Verna N, Carroll M, Moore FD, Hechtman HB: Ischaemia-reperfusion is an event triggered by immune complexes and complement. Br J Surg. 2003, 90: 1470-1478. 10.1002/bjs.4408.View ArticlePubMedGoogle Scholar
- Elvington A, Atkinson C, Zhu H, Yu J, Takahashi K, Stahl GL, Kindy MS, Tomlinson S: The alternative complement pathway propagates inflammation and injury in murine ischemic stroke. J Immunol. 2012, 189: 4640-4647. 10.4049/jimmunol.1201904.PubMed CentralView ArticlePubMedGoogle Scholar
- Miwa T, Sato S, Gullipalli D, Nangaku M, Song WC: Blocking properdin, the alternative pathway, and anaphylatoxin receptors ameliorates renal ischemia-reperfusion injury in decay-accelerating factor and CD59 double-knockout mice. J Immunol. 2013, 190: 3552-3559. 10.4049/jimmunol.1202275.PubMed CentralView ArticlePubMedGoogle Scholar
- He S, Atkinson C, Qiao F, Cianflone K, Chen X, Tomlinson S: A complement-dependent balance between hepatic ischemia/reperfusion injury and liver regeneration in mice. J Clin Invest. 2009, 119: 2304-2316.PubMed CentralPubMedGoogle Scholar
- Bouma HR, Carey HV, Kroese FG: Hibernation: the immune system at rest?. J Leukoc Biol. 2010, 88: 619-624. 10.1189/jlb.0310174.View ArticlePubMedGoogle Scholar
- Mollnes TE, Garred P, Bergseth G: Effect of time, temperature and anticoagulants on in vitro complement activation: consequences for collection and preservation of samples to be examined for complement activation. Clin Exp Immunol. 1988, 73: 484-488.PubMed CentralPubMedGoogle Scholar
- Bonaparte RS, Hair PS, Banthia D, Marshall DM, Cunnion KM, Krishna NK: Human astrovirus coat protein inhibits serum complement activation via C1, the first component of the classical pathway. J Virol. 2008, 82: 817-827. 10.1128/JVI.01847-07.PubMed CentralView ArticlePubMedGoogle Scholar
- Mauriello CT, Pallera HK, Sharp JA, Woltmann JL, Qian S, Hair PS, van der Pol P, van Kooten C, Thielens NM, Lattanzio FA, Cunnion KM, Krishna NK: A novel peptide inhibitor of classical and lectin complement activation including ABO incompatibility. Mol Immunol. 2013, 53: 132-139. 10.1016/j.molimm.2012.07.012.PubMed CentralView ArticlePubMedGoogle Scholar
- Cunnion KM, Lee JC, Frank MM: Capsule production and growth phase influence binding of complement to Staphylococcus aureus. Infect Immun. 2001, 69: 6796-6803. 10.1128/IAI.69.11.6796-6803.2001.PubMed CentralView ArticlePubMedGoogle Scholar
- Hair PS, Echague CG, Rohn RD, Krishna NK, Nyalwidhe JO, Cunnion KM: Hyperglycemic conditions inhibit C3-mediated immunologic control of Staphylococcus aureus. J Transl Med. 2012, 10: 35-10.1186/1479-5876-10-35.PubMed CentralView ArticlePubMedGoogle Scholar
- Hair PS, Gronemus JQ, Crawford KB, Salvi VP, Cunnion KM, Thielens NM, Arlaud GJ, Rawal N, Krishna NK: Human astrovirus coat protein binds C1q and MBL and inhibits the classical and lectin pathways of complement activation. Mol Immunol. 2010, 47: 792-798. 10.1016/j.molimm.2009.10.006.View ArticlePubMedGoogle Scholar
- Gronemus JQ, Hair PS, Crawford KB, Nyalwidhe JO, Cunnion KM, Krishna NK: Potent inhibition of the classical pathway of complement by a novel C1q-binding peptide derived from the human astrovirus coat protein. Mol Immunol. 2010, 48: 305-313. 10.1016/j.molimm.2010.07.012.View ArticlePubMedGoogle Scholar
- Serruto D, Rappuoli R, Scarselli M, Gros P, van Strijp JA: Molecular mechanisms of complement evasion: learning from staphylococci and meningococci. Nat Rev Microbiol. 2010, 8: 393-399. 10.1038/nrmicro2366.View ArticlePubMedGoogle Scholar
- Guo RF, Ward PA: Role of C5a in inflammatory responses. Annu Rev Immunol. 2005, 23: 821-852. 10.1146/annurev.immunol.23.021704.115835.View ArticlePubMedGoogle Scholar
- Gonzalez-Ibarra FP, Varon J, Lopez-Meza EG: Therapeutic hypothermia: critical review of the molecular mechanisms of action. Front Neurol. 2011, 2: 4-PubMed CentralView ArticlePubMedGoogle Scholar
- Moore EM, Nichol AD, Bernard SA, Bellomo R: Therapeutic hypothermia: benefits, mechanisms and potential clinical applications in neurological, cardiac and kidney injury. Injury. 2011, 42: 843-854. 10.1016/j.injury.2011.03.027.View ArticlePubMedGoogle Scholar
- Robertson NJ, Tan S, Groenendaal F, van Bel F, Juul SE, Bennet L, Derrick M, Back SA, Valdez RC, Northington F, Gunn AJ, Mallard C: Which neuroprotective agents are ready for bench to bedside translation in the newborn infant?. J Pediatr. 2012, 160: 544-552.e544. 10.1016/j.jpeds.2011.12.052.PubMed CentralView ArticlePubMedGoogle Scholar
- Kodama H, Kodama Y, Shinozawa S, Kanemaru R, Todaka K, Mitsuyama Y: Temperature effect on serum protein binding kinetics of phenytoin in monotherapy patients with epilepsy. Eur J Pharm Biopharm. 1999, 47: 295-298. 10.1016/S0939-6411(99)00008-9.View ArticlePubMedGoogle Scholar
- Muller S, Rother U, Westerhausen M: Complement activation by cryoglobulin. Evidence for a pathogenic role of an IgG (kappa) cryoglobulin in cutaneous vasculitis. Clin Exp Immunol. 1976, 23: 233-241.PubMed CentralGoogle Scholar
- Moon R, Pritts TA, Parikh AA, Fischer JE, Salzman AL, Ryan M, Wong HR, Hasselgren PO: Stress response decreases the interleukin-1beta-induced production of complement component C3 in human intestinal epithelial cells. Clin Sci (Lond). 1999, 97: 331-337. 10.1042/CS19990015.View ArticleGoogle Scholar
- Loetscher H, Steinmetz M, Lesslauer W: Tumor necrosis factor: receptors and inhibitors. Cancer Cells. 1991, 3: 221-226.PubMedGoogle Scholar
- Moore FD, Warner KG, Assousa S, Valeri CR, Khuri SF: The effects of complement activation during cardiopulmonary bypass. Attenuation by hypothermia, heparin, and hemodilution. Ann Surg. 1988, 208: 95-103. 10.1097/00000658-198807000-00014.PubMed CentralView ArticlePubMedGoogle Scholar
- Bruins P, te Velthuis H, Eerenberg-Belmer AJ, Yazdanbakhsh AP, de Beaumont EM, Eijsman L, Trouwborst A, Hack CE: Heparin-protamine complexes and C-reactive protein induce activation of the classical complement pathway: studies in patients undergoing cardiac surgery and in vitro. Thromb Haemost. 2000, 84: 237-243.PubMedGoogle Scholar
- Rice JE, Vannucci RC, Brierley JB: The influence of immaturity on hypoxic-ischemic brain damage in the rat. Ann Neurol. 1981, 9: 131-141. 10.1002/ana.410090206.View ArticlePubMedGoogle Scholar
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