Figure 3From: Clinical hypothermia temperatures increase complement activation and cell destruction via the classical pathway C1s activation by heat-aggregated IgG for increasing temperatures. One μg of partially purified human C1 was incubated with heat-aggregated IgG at 31-41 C for 0–90 minutes. C1s activation, as measured by generation of C1s heavy and light chains by Western blot methodology, was quantified by Odyssey imaging and demonstrated decreased activation of C1s at therapeutic hypothermia temperatures (ANOVA P < 0.01), with a 5-fold decrease at 31°C vs. 37°C (*t-test P < 0.01). Data are mean ± SEM for 3 independent experiments.Back to article page