The BAG3 gene variants in Polish patients with dilated cardiomyopathy: four novel mutations and a genotype-phenotype correlation
- Maria Franaszczyk1,
- Zofia T Bilinska2,
- Małgorzata Sobieszczańska-Małek3,
- Ewa Michalak2,
- Justyna Sleszycka4,
- Agnieszka Sioma2,
- Łukasz A Małek5,
- Dorota Kaczmarska2,
- Ewa Walczak6,
- Paweł Włodarski7,
- Łukasz Hutnik7,
- Blanka Milanowska2,
- Zofia Dzielinska8,
- Grzegorz Religa9,
- Jacek Grzybowski4,
- Tomasz Zieliński3 and
- Rafal Ploski10Email author
© Franaszczyk et al.; licensee BioMed Central Ltd. 2014
Received: 19 February 2014
Accepted: 25 June 2014
Published: 9 July 2014
BAG3 gene mutations have been recently implicated as a novel cause of dilated cardiomyopathy (DCM). Our aim was to evaluate the prevalence of BAG3 mutations in Polish patients with DCM and to search for genotype-phenotype correlations.
We studied 90 unrelated probands by direct sequencing of BAG3 exons and splice sites. Large deletions/insertions were screened for by quantitative real time polymerase chain reaction (qPCR).
We found 5 different mutations in 6 probands and a total of 21 mutations among their relatives: the known p.Glu455Lys mutation (2 families), 4 novel mutations: p.Gln353ArgfsX10 (c.1055delC), p.Gly379AlafsX45 (c.1135delG), p.Tyr451X (c.1353C>A) and a large deletion of 17,990 bp removing BAG3 exons 3–4. Analysis of mutation positive relatives of the probands from this study pooled with those previously reported showed higher DCM prevalence among those with missense vs. truncating mutations (OR = 8.33, P = 0.0058) as well as a difference in age at disease onset between the former and the latter in Kaplan-Meier survival analysis (P = 0.006). Clinical data from our study suggested that in BAG3 mutation carriers acute onset DCM with hemodynamic compromise may be triggered by infection.
BAG3 point mutations and large deletions are relatively frequent cause of DCM. Delayed DCM onset associated with truncating vs. non-truncating mutations may be important for genetic counseling.
Dilated cardiomyopathy (DCM) is a major cause of chronic heart failure and the most common indication for cardiac transplantation . In a substantial number of cases DCM is familial with autosomal dominant inheritance. Whereas a large number of genes have been shown to harbor mutations causing DCM  novel disease loci are continuously being reported. One interesting gene which recently has been implicated as a novel DCM locus is BAG3[3, 4].
BAG3 belongs to a family of co-chaperones playing an anti-apoptotic role with their BAG domains binding the ATPase domain of heat shock proteins 70 (Hsc70/Hsp70) . BAG3 is expressed primarily in skeletal muscle cells and cardiomyocytes, where it localizes within the Z-disc and probably acts as a signaling molecule . Mice with homozygous knockout of the BAG3 gene have degeneration of muscle fibers in striated muscle with apoptosis leading to fulminant skeletal myopathy and cardiomyopathy causing death approximately four weeks after birth . BAG3-null zebrafish demonstrated myocardial changes resembling human DCM . Since BAG3 has an anti-apoptotic activity, the DCM-associated BAG3 mutations may act through increasing cardiomyocytes’ sensitivity to apoptosis as shown experimentally for metabolic  or mechanical stress .
A specific BAG3 mutation virtually always occurring de novo (Pro209Leu) causes a severe childhood myofibrillar myopathy which is regarded as a distinct disease from that caused by other known BAG3 mutations [9–11].
The purpose of our study was to evaluate the prevalence of BAG3 mutations in Polish patients with DCM and to search for genotype-phenotype correlations.
Patients and families
Screening for BAG3 mutations
DNA was extracted from the peripheral blood by phenol extraction. We screened the entire coding region together with splice sites of BAG3 by direct Sanger sequencing. For all BAG3 exons PCR was carried out with primers listed in Additional file 1: Table S1. The PCR conditions were: 5 min of initial denaturation at 95°C, followed by 32 cycles of 30 sec at 95°C, 55 sec at 60-68°C, 1 min at 72°C and final extension of 10 min at 72°C. PCR products were examined on 2.5% agarose gels and then sequenced using a 3500×L Genetic Analyzer (Applied Biosystems, Foster City, CA, USA) and BigDye Terminator v3.1 Cycle Sequencing Kit (Applied Biosystems) according to the manufacturer’s instructions. The results were analyzed with Variant Reporter 1.1 Software (Applied Biosystems).
Screening for large deletions in BAG3
The screening for large deletions in BAG3 was performed by quantitative PCR (qPCR) using Applied Biosystems 7500 Real Time PCR System and MESA GREEN MasterMix Plus, Low ROX (Eurogentec, Belgium). The PCR conditions were: 10 min of initial denaturation at 95°C, followed by 40 cycles of 15 sec at 95°C and 1:45 min at 60°C. Albumin gene (ALB) was used as a reference. All qPCR analyses were run in duplicates. Copy number was calculated by delta delta Ct method i.e. ∆∆Ct = (Ct BAG3 - Ct ALB)test - (Ct BAG3 - Ct ALB)wild type where Ct denotes mean cycle number in which threshold value of fluorescence was recorded for the BAG3 or ALB primers for the reference (a sample without deletion, i.e. ‘wild type’) and tested (‘test’) sample, respectively. For Ct determination the default method available on the instrument was used. ∆∆Ct > 0.8 was regarded as indicative of deletion. Subsequent fine mapping of the detected deletion was performed in a similar way using a stepwise approach. The sequences of BAG3 and ALB primers used at both stages of analysis are listed in Additional file 2: Table S2. Primers used for final PCR-amplification and sequencing of the breakpoint region were: BAG3intron2/3 5′TGC TCT CAA TTT CGA GGT GA 3′ and BAG3del 5′ CGG GAG AAT CAT GAG GTC AG 3′ (the forward and the reverse primer, respectively). The same primers as those used for sequencing of the breakpoint region were also used for screening of family members for the presence of the deletion.
Statistical significance of difference in DCM prevalence among subjects with truncating vs. non-truncating BAG3 mutations was performed by Chi square test. Age at DCM onset in these groups was analyzed by Kaplan-Meier survival curves and Cox’s F-Test. All analyses were performed using Statistica software package (StatSoft).
Analysis of probands
To search for large deletions in BAG3 we performed qPCR comparing dosage of DNA within BAG3 exons with that a reference gene (albumin). In one sample (proband from the DCM-1 family) using primers RT_Exon3 and RT_Exon4.1 located in exons 3 and 4 respectively we observed shifts in amplification curves consistent with presence of a heterozygous deletion (∆∆Ct = 0.92).
Next, we carried out qPCR experiments aimed at stepwise narrowing of the deleted region. Eight such experiments were performed and they eventually allowed to narrow down the deletion to a region sufficiently short to design PCR primers likely to amplify the deletion breakpoint. The PCR product was obtained and its identity was verified by sequencing which also demonstrated that the deletion encompassed a region of 17,990 bp including 3 and 4 exons of BAG3 and extending into the telomeric end in the direction of the INPP5F gene (Figure 2D). We noted that the 5 bp of the DNA sequence directly adjacent to the centromeric end of the deleted fragment was identical to the sequence at the telomeric end of the deleted fragment (AGTGG in both cases, Figure 2D).
The prevalence among probands of all detected BAG3 mutations was 6/90 or 6.7% with 95% confidence interval from 3.1% to 13.8%.
Clinical and histopathological findings among the probands
Among the six probands with BAG3 mutations one had an acute onset with a fulminant course of the disease (DCM-15 III-7) and died awaiting heart transplantation (HTX) while on ventricular assist devices within three months from the diagnosis. Three probands (DCM-18 III-2, DCM-1 IV-1, DCM-71 III-4) received HTX after 60, 108 or 196 months from the diagnosis, respectively. Two probands experienced partial recovery: one remains stable after 108 months with LVEF 36% and another one with 2 months follow-up’ time experienced significant improvement in LV systolic function (LVEF 51%) while on standard treatment of heart failure. Clinical characteristics of probands are given in Additional file 3: Table S3.
Histopathological analysis in patients with the Glu455Lys variant (DCM-16 III-1) was performed within one month after the onset of acute heart failure. The findings were non specific with features of cellular hypertrophy, myocardial cell degeneration and interstitial fibrosis as usually found in DCM. In the specimen from the patient with the Gly379AlafsX45 variant (DCM-15 III-5) tissue severe myocytolysis with scarce inflammatory infiltrate was found.
Analysis of family members – clinical findings
In addition to six probands we have identified 21 relatives with BAG3 mutations. Eleven (52.4%) of these subjects had DCM with the mean age at onset of 36 years (range 15–53). There were two deaths: one female died suddenly at 58 years after 60 months from the diagnosis and there was one heart failure death at 42 years after 120 months from the diagnosis in a male patient. Significant left ventricular dysfunction at the end of follow-up with LVEF ≤ 45% was present in 9 relatives (42.9%). In two mutation carriers (9.5%) an improvement in LV function was observed over the follow-up while on standard treatment for heart failure. Persistent normal LV function was found in 10 (47.6%) carriers whose mean age was 29.2 years (range 20–53). Clinical characteristics of relatives with BAG3 mutations are given in Additional file 3: Table S3.
CMR study in DCM-15 III-2 (Gly379AlafsX45), performed within one month after the onset of symptoms showed dilated hypocontractile left ventricle and the presence of CMR diagnostic criteria for myocarditis . In particular, T2-weighted images demonstrated global myocardial signal intensity (SI) increase (>2) in comparison to skeletal muscle indicating global edema (Additional file 4: Figure S1A). There was also an increased global myocardial early gadolinium enhancement ratio (>4) in comparison to skeletal muscle in* gadolinium-enhanced T1-weighted images indicating global myocardial hyperemia. Late gadolinium enhancement (LGE) revealed intramyocardial foci in the interventricular septum and at the low junction point of the right and left ventricle indicating myocardial fibrosis (Additional file 4: Figure S1B). At the time of CMR study, serological examinations showed positive serum IgM Lyme titres and borderline IgG, positive IgG antyParvovirus B19 titres and detectable human herpes virus 6 (HHV6) genome in the serum. The patient received gammaglobulin 0.1 g/kg i.v. along with doxycycline 100 mg PO bid for 21 days leading to substantial clinical improvement (a rise in LVEF up to 36%).
Analysis of family members – genotype dependent penetrance of BAG3 related DCM
Presence of DCM among subjects with truncating and non-truncating BAG3 mutations (probands excluded)
Truncating BAG3 mutations
While studying a cohort of 90 adult unrelated DCM patients and their relatives we found BAG3 mutations in 6 probands and 21 family members. Four of the observed mutations were novel: Gln353ArgfsX10 (c.1055delC), Gly379AlafsX45, (c.1135delG), Tyr451X (c.1353C>A) and a large deletion removing 17,990 bp. Analysis of affection status in BAG3 mutations carriers among relatives of the probands from our cohort together with those reported previously [3, 4] showed difference in age related penetrance which, interestingly, suggested later onset of disease in those with non-truncating vs. truncating mutations.
The BAG3 Glu455Lys (rs397516881) has been reported previously as pathogenic although this was based on a single report [3, 4] and the ClinVar database  describes the variant as having uncertain significance. The conclusion on Glu455Lys pathogenicity was based on a single family in which this variant was found in five individuals four of whom had DCM [3, 4]. Our finding of rs397516881 in two apparently unrelated DCM probands and three affected family members together with its lack in NHLBI GO exome sequencing project (ESP) , 1000 genomes databases  and our in-house exome database of 250 Poles argues for genuine association of BAG3 Glu455Lys with DCM. However, this conclusion would certainly be strengthened by additional data from other populations.
The Gln353ArgfsX10 (c.1055delC), Gly379AlafsX45 (c.1135delG) and the 17,990 bp deletion removing exons 3–4 are likely to be pathogenic as they are predicted to remove a larger C terminal part of the BAG3 protein than the two previously reported pathogenic BAG3 mutations: R395GfsX48 and S385QfsX56 . This argument does not apply to Tyr451X which is the most C terminal (the least truncating) BAG3 mutation reported so far. However, Tyr451X is also likely to be pathogenic as it removes more than half of the single BAG domain present in the BAG3 protein. The deleted part contains whole alpha3 helix and a significant part of the alpha2 helix, both of which are responsible for binding between BAG3 and Hsc710/Hsp70 . The part of alpha2 and the whole alpha3 helix which are deleted by Tyr451X mutation contain numerous aminoacids highly conserved both among BAG domains of different human BAG proteins  and between BAG3 proteins of different species (Additional file 5: Figure S2). Finally, the BAG3 Tyr451X mutation, similar as Gln353ArgfsX10 (c.1055delC), Gly379AlafsX45 (c.1135delG), has not been observed in NHLBI GO exome sequencing project (ESP) , 1000genomes database , ClinVar databases  or our in-house exome database of 250 Poles. Whereas all these findings suggest pathogenicity, as recently emphasized for other variants [20, 21], more data is needed for a firm conclusion.
Among the novel BAG3 mutations the most interesting is the large deletion of 17,990 bp which removes exons 3–4 and a chromosome fragment extending in the direction of the INPP5F gene. This deletion, together with the deletion of 8,733 bp described by Norton et al.  suggests that the 3′ part of the BAG3 locus may be prone to structural rearrangements. The 17,990 bp deletion probably originated due to microhomology at the breakpoints and thus may be recurrent . These observations highlight the necessity for screening BAG3 for copy number variations (CNV) variants in addition to point mutations.
The prevalence of BAG3 defects in our cohort was relatively high (6/90 or 6.7%) being comparable to the prevalence of mutations in LMNA (~6%) which has been regarded as the most frequently mutated locus in DCM [23, 24]. Thus, the BAG3 gene emerges as a major DCM locus. Although its role is clearly smaller than that of TTN, whose mutations have recently been shown to occur in up to 25% of DCM patients  our results indicate that, at least in Polish population, a systematic screening of BAG3 should be offered to DCM patients.
The findings that truncating BAG3 mutations cause disease with later onset than missense variants may be important for genetic counselling. Although it should be confirmed by a study specifically addressing disease severity, our results suggest that missense BAG3 mutations may have a stronger pathogenic effect than the truncating variants. Interestingly, that would contrast with observations for the LMNA gene whose truncating variants were recently associated with a more severe DCM . As shown for the LMNA gene , a likely main effect of truncating mutations is the loss of function, whereas missense variants may in addition (or alternatively) exert dominant negative effects. Pathogenicity of BAG3 haploinsufficiency is supported by DCM association shown for a number of truncating variants, in particular the severely truncating BAG3 Arg90X mutation . However, earlier onset of DCM suggestive of a more severe phenotype associated with non-truncating mutations found in our study indicates that at least some BAG3 missense variants exert dominant-negative effects. This notion is consistent with a distinct and severe phenotype (childhood onset myopathy) consistently observed in patients with the BAG3 p.Pro209Leu mutation [9–11].
Clinical data from our study suggest that in presence of BAG3 defects stress may trigger acute onset DCM with hemodynamic compromise, which is consistent with in vitro studies implicating BAG3 in the control of apoptosis and response to stress stimuli [7, 8]. A BAG3 mutation carrier with history of acute heart failure (DCM-15 III-2) fulfilled CMR diagnostic criteria for myocarditis with serological evidence of acute Lyme disease and past Parvovirus B19 infection, and a detectable HHV6 genome in the blood. Intramyocardial foci of late gadolinium enhancement in the interventricular septum were previously observed in patients with myocarditis associated with human HHV6 infection or Lyme disease [12, 28–30]. Moreover, all severely affected BAG3 mutation carriers of the DCM-18 family had disease onset related to the influenza of 1988 along with two of deceased first-degree members of the family who developed progressive heart failure leading to death. Furthermore, all subjects with BAG3 mutations who had acute onset of heart failure following viral-like illness had very low LVEF (10-22%), that is consistent with poor response to any pathogen-related stress.
In conclusion, by studying Polish patients we found that BAG3 mutations are relatively frequent cause of DCM. We report four novel pathogenic BAG3 variants including a large deletion and show, for the first time, that truncating BAG3 variants are associated with DCM characterized by later onset than missense variants. Clinical data from our study suggest that in BAG3 mutation carriers infection may trigger acute onset DCM with hemodynamic compromise. Despite the limitations of our study such as relatively small number of subjects and a retrospective design our results add to the knowledge on BAG3 related diseases and, if replicated in additional cohorts, may be important for genetic counseling.
The study was supported by Polish National Science Centre (NCN) grant 2011/01/B/NZ4/03455.
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