Novel patient-derived xenograft mouse model for pancreatic acinar cell carcinoma demonstrates single agent activity of oxaliplatin
- Jason C. Hall†1Email author,
- Laura A. Marlow†1,
- Adam C. Mathias4,
- Louis K. Dawson4,
- William F. Durham4,
- Kenneth A. Meshaw4,
- Robert J. Mullin4,
- Aidan J. Synnott4,
- Daniel L. Small4,
- Murli Krishna2,
- Daniel von Hoff5,
- Julia Schüler4,
- Steven N. Hart6,
- Fergus J. Couch6,
- Gerardo Colon-Otero3 and
- John A. Copland1
© Hall et al. 2016
Received: 21 January 2016
Accepted: 25 April 2016
Published: 10 May 2016
Pancreatic acinar cell carcinoma (PACC) is a rare malignancy, accounting for <1 % of all pancreatic neoplasms. Very few retrospective studies are available to help guide management. We previously reported the case of a patient with metastatic PACC who achieved prolonged survival following doxorubicin treatment. Personalized treatment was based on molecular and in vitro data collected from primary cells developed from their liver metastasis. We now report the characterization of a patient derived tumor xenograft (PDTX) mouse model that originated from this patient’s PACC liver metastasis.
Fragments of biopsy tissue (5 mm3) from PACC liver metastasis were implanted into athymic nude mice. Tumors were grown and passaged from the host mice into new mice to be tested for therapeutic response. Immuno-histochemical (IHC) biomarkers were used to confirm that the PDTX model represents human PACC. The antitumor activities of multiple drugs (5-FU, irinotecan, oxaliplatin, gemcitabine, bevacizumab, erlotinib, doxorubicin and imatinib) were tested. Tumor size was measured over 74 days or until they reached an endpoint volume of ~800 mm3. Tests to measure serum lipase levels and histological analyses of tumor tissues were also conducted to assess PACC progression and re-differentiation.
The model presented here expresses the same IHC markers found in human PACC. In the chemotherapy study, oxaliplatin produced a prolonged durable growth response associated with increased apoptosis, decreased serum lipase levels and increased healthy acinar cells. Bevacizumab also produced a significant growth response, but the effect was not prolonged as demonstrated by oxaliplatin treatment. The other chemotherapies had moderate to little effect, particularly after treatment ceased. Mutations in DNA repair genes are common in PACC and increase tumor susceptibility to oxaliplatin. To explore this we performed IHC and found no nuclear expression of BRCA2 in our model, indicating a mutation affecting nuclear localization. Gene sequencing confirms BRCA2 has a homozygous gene deletion on Exon 10, which frequently causes a protein truncation.
In summary, we report the development and characterization of the first and only preclinical PACC PDTX model. Here we show sustained anti-tumor activity of single agent oxaliplatin, a compound that is more effective in tumors that harbor mutations in DNA repair genes. Our data shows that BRCA2 is mutated in our PACC model, which could contribute to the oxaliplatin sensitivity observed. Further studies on this rare PACC model can serve to elucidate other novel therapies, biomarkers, and molecular mechanisms of signaling and drug resistance.
KeywordsOxaliplatin Patient derived tumor xenograft Tumor Pancreatic acinar cell carcinoma Chemotherapy Individualized medicine Precision medicine BRCA2
Pancreatic acinar cell carcinoma (PACC) is a rare, frequently lethal disease, accounting for less than 1 % of all pancreatic neoplasms tumors [1–4]. A retrospective series of 672 patients with PACC reported a 47 months median survival . A review of patients seen at Memorial Sloan Kettering from 1981 to 2001 found that the median survival of patients with metastasis was 14 months as compared to 38 months in the absence of metastasis . A follow up retrospective study reviewing patients seen from 2000 to 2011 found a median survival of 57 months for patients with localized resected tumors and a 19 months median survival in patients with metastatic disease .
Patients with PACC frequently present with abdominal pain and bloating as the dominant symptoms, and in some cases their initial clinical diagnosis given was acute pancreatitis [1, 7, 8]. Elevation of serum lipase levels can be associated with systemic fat necrosis, a significant cause of morbidities in PACC patients [9–12]. However, elevated serum lipase and amylase levels can be seen in both PACC and acute pancreatitis leading to difficulties in establishing a correct diagnosis [13, 14]. Reliable markers of PACC have been slowly emerging such as carcinoembryonic antigen (CEA) , cytokeratin 18 (CK18)  and B-cell lymphoma/leukemia 10 (BCL10) [17, 18] allowing us to distinguish acinar cell carcinoma from normal acinar cells or other pancreatic cancers through histology.
The genetic and molecular abnormalities that lead to PACC have not been fully identified. Recent studies have found that genes regulating DNA repair may be mutated in PACC patients. DNA repair mutations were noted in 45 % of PACC tumors, BRCA2 being the most common gene, followed by BRCA1 and ATM20 [3, 6, 19]. Previously, we reported on a case of a PACC patient receiving personally designed treatments based on the genetic and molecular profiles of his tumor . We cultured the patient’s tumor biopsies into primary cell lines and treated them with different chemotherapy agents . The outcome was that a DNA replication inhibitor, (irinotecan), a DNA intercalating agent and transcription inhibitor (doxorubicin), and a tyrosine kinase inhibitor (imatinib) were among the most effective agents against his tumor cells in cell culture . As a result of the observations seen in vitro, liposomal doxorubicin was administered and the patient had evidence of a sustained clinical response throughout the treatment regimen .
Here, we report the characterization of a PACC patient derived tumor xenograft (PDTX) mouse model (PA-018) from the patient’s tumor biopsy. With the use of PA-018, multiple chemotherapies (5-FU, oxaliplatin, gemcitabine, liposomal doxorubicin) and targeted agents (irinotecan, bevacizumab, erlotinib, imatinib) were tested in vivo. Of the therapies tested, oxaliplatin was the most promising and demonstrated sustained antitumor activity after only 3 weekly treatments. Therefore, evaluation of potential effective treatments using this PDTX model is a viable technology that may facilitate the discovery of effective treatments against this rare tumor. We provide the first human derived PACC tumor model now available to the PACC scientific community.
Development of PDTX model
Biopsy tissue from PACC liver metastasis was implanted subcutaneously into two 5 week old anesthetized athymic nude female mice strain #069 (Harlan Laboratories, Indianapolis, IN) under IACUC approved procedures. Implanted tumors were harvested and frozen as 5 mm3 fragments in 10 % DMSO-DMEM media and multiple “passages” were continued in athymic nude mice. Applying this technique to the patient’s tumor has created a renewable source of PACC tissue and a representative in vivo model to test promising drugs. This PACC PDTX model is available at Charles Rivers (CR) Discovery Services (Morrisville, NC).
In vivo implantation and tumor growth
For the in vivo study, 6 week old female athymic nude mice strain #490 (CR Discovery Services) were subcutaneously implanted with 5 mm3 tumor fragments into the right flanks. They were fed ad libitum NIH 31 Modified and Irradiated Lab Diet® consisting of 18.0 % crude protein, 5 % crude fat, and 5 % crude fiber and housed on irradiated Enrich-o’cobs™ Laboratory Animal Bedding in static microisolators on a 12-hour light cycle at 20–22 °C (68–72 °F) and at 40–60 % humidity. CR Discovery Services specifically complies with the recommendations of the Guide for Care and Use of Laboratory Animals with respect to restraint, husbandry, surgical procedures, feed and fluid regulation, and veterinary care. The animal care and use program at CR Discovery Services is accredited by the Association for Assessment and Accreditation of Laboratory Animal Care International, which assures compliance with accepted standards for the care and use of laboratory animals.
Test articles and dosing regimens
The following therapies were prepared on each day of dosing as follows: 5-Fluorouracil or 5-FU (TEVA Pharmaceuticals, 50 mg/mL, Lot# 6102655) was diluted to 10 mg/mL with sterile 5 % dextrose in water (D5 W) and administered at 100 mg/kg intra-peritoneal (ip) weekly for 3 weeks. Irinotecan solution for injection (Sandoz Pharmaceuticals, Inc., 20 mg/mL, Lot# CF0165) was diluted to 10 mg/mL with D5 W and administered at 100 mg/kg ip once weekly for 3 weeks. An aliquot of oxaliplatin stock (Eloxatin®, Sanofi Aventis, 5 mg/mL, Lot# CH630) was diluted to 1 mg/mL with sterile D5 W, which provided a 10 mg/kg dosage that was administered ip once weekly for 3 weeks. Gemcitabine (Gemzar®, Eli Lilly, Lot# A906313D) was reconstituted to 12 mg/mL with sterile saline (0.9 % NaCl) and 120 mg/kg was administered ip daily every 3 days for a total of four doses. An aliquot of Bevacizumab stock (Avastin®, Genentech, Lot# 956583, 25 mg/mL) was diluted to 0.5 mg/mL with saline, which provided a 5 mg/kg dosage that was administered ip twice weekly for five weeks. Liposomal doxorubicin (Doxil, Sequus Pharmaceuticals, Inc., 2 mg/mL, Lot# 1107161) was diluted to 0.3 mg/mL in saline, which provided a 3 mg/kg dosage that was administered intravenously (i.v.) once weekly for 3 weeks. Imatinib mesylate (Gleevec®, Novartis Pharmaceuticals Corp, 100 mg tablets) dosing suspensions were prepared by resuspending the required amount of tablets in sterile water for injection to yield a final concentration of 10 mg/mL and then administered orally (po) at 100 mg/kg once daily for 28 days. Dosing suspensions of erlotinib (Tarceva®, OSI Pharmaceuticals, Inc., Lot# 1121701CW, 100 mg tablets) were prepared by resuspending the required amount of tablets in 1 % CMC : 0.1 % Tween80 in sterile water to produce a final concentration of 8 mg/mL and then administered orally at of 80 mg/kg once daily for 15 days. In all groups, the dosing volume of 10 mL/kg (0.2 mL/20 g mouse) was scaled to the weight of each individual animal.
Test animals were observed frequently for any overt signs of adverse, treatment-related side effects, and clinical signs of toxicity were recorded. Test animals were also weighed twice weekly and any animal that exceeded the limits for acceptable body weight (BW) loss was euthanized. Dosing was suspended in any group that exceeded the limits for acceptable mean BW loss. If mean BW recovered, then dosing may be resumed in that group, but at a lower dosage or less frequent dosing schedule. Acceptable toxicity for the maximum tolerated dose (MTD) was defined as group mean BW loss of less than 20 % during the test, and no more than 10 % TR mortality. A death was classified as TR if it was attributable to treatment side effects as evidenced by clinical signs and/or necropsy, or due to unknown causes during the dosing period or within 14 days of the last dose. A death was classified as NTR if there is no evidence that the death was related to treatment side effects.
Statistical analysis and graphical presentations
Prism (GraphPad) for Windows 6.02 was used for all statistical analysis and graphical presentations. The logrank test was employed to assess the significance of the difference between the overall survival experiences of two groups. The logrank test analyzed the individual TTEs for all animals in a group, except those lost to the study due to NTR deaths. The two-tailed statistical analysis was conducted at P = 0.05. Prism reports results as non-significant (ns) at P > 0.05, significant (symbolized by “*”) at 0.01 < P ≤ 0.05, very significant (“**”) at 0.001 < P ≤ 0.01 and extremely significant (“***”) at P ≤ 0.001. Since the logrank test is a test of significance and does not provide an estimate of the size of the difference between groups, all levels of significance are reported as either significant or non-significant within the context of this report. When an animal exited the study due to tumor size or TR death, the final tumor volume recorded for the animal was included with the data used to calculate the median volume at subsequent time points. Tumor growth curves were truncated when the tumors in more than 50 % of the assessable animals in a group have grown to the endpoint volume or treatment exceeded the MTD (≥20 % body weight loss or >10 % TR related mortality).
Blood was collected sublingually under no anesthesia at pre-dose and day 15 for n = 5 mice per group in EDTA collection tubes. Serum was collected and analyzed by IDEXX laboratories (Westbrook, Maine) for serum lipase levels.
Immunohistochemistry (IHC) and immunofluorescence (IF)
Formalin-fixed tissues were collected and embedded into paraffin. A tissue microarray (TMA) was constructed for all tumor tissues from each of the treatment groups and used for IHC analysis. TMA tissues were cut into 5 mm sections, deparaffinized, hydrated, antigen retrieved and blocked with diluent that contained Background Reducing Components (Dakocytomation, Denmark). Immunostaining was done on either the TMA or PA-018 alone with the following: carcinoembryonic antigen (CEA), neuron specific enolase (NSE), Chromogranin A (CgA), and cytokeratin 19 (CK19) [1:100, anti-mouse with rodent block (Dakocytomation)]; Mist-1 [1:2000] and cleaved caspase-3 (CC3) [1:100 hi pH, anti-rabbit (Cell Signaling, Beverly, MA)]; amylase [1:1000, anti-rabbit (Sigma-Aldrich, St.Louis, MO)]; lipase [1:1600], BRCA1 [1:100], and Collagen I [1:1500, anti-rabbit (Abcam, Cambridge, MA)]; BCL-10 [1:200, anti-rabbit] and CD31 [1:100, anti-goat (Santa Cruz, CA)]; BRCA2 [1:500, anti-mouse (R&D systems)]. For IHC the Envision Dual Labeled Polymer kit (DakoCytomation) was used according to the manufacturer’s instructions and then lightly counterstained with Gill I hematoxylin (Sigma-Aldrich) before dehydration and mounting. Images were obtained using Scanscope XT (Aperio Technologies, Vista, CA) and the staining of the TMA punches were scored using an algorithm in the Imagescope software (Aperio Technologies) created by a histologist based upon signal intensity (0, 1+ , 2+ , 3+) and percentage. For immunofluorescence, tissue was incubated with AlexaFlour 594-conjugated secondary antibody (Invitrogen) and mounted with Vectashield containing DAPI (Vector Laboratories, Burlingame, CA). Images were acquired on a Zeiss LSM 880 confocal laser scanning microscope (Carl Zeiss MicroImaging, Inc, Thornwood, NY).
Gene mutation analysis
DNA from a fresh frozen sample of PA-018 was isolated and underwent custom capture (eArray; Agilent, Santa Clara, CA) of the coding region and intron/exon boundaries of coding exons for BRCA2. Products from each capture reaction were sequenced on a HiSeq 2000 (Illumina, San Diego, CA) and analyzed as described by Couch et al. .
Characterization of the PACC PDTX mouse model
IHC characterization of PA-018 showed a reduction in cytoplasmic expression of amylase and lipase as compared to the normal human pancreas, while the pancreatic acinar marker, Mist-1 , was widely expressed in the tumor tissue (Fig. 1c, panels i–iii). PA-018 also expressed previously documented markers of PACC, including CEA , CK18  and BCL10 [17, 18] (Fig. 1c, panels iv, vi) and had a lack of cytokeratin 19 (CK19) expression, a marker of ducts and pancreatic ductal tumors  (Fig. 1c, panel vii). Since PACC can have heterogeneous tumor populations, which include neuroendocrine derived cancer cells, we stained for neuroendocrine markers, such as neuron specific enolase (NSE) and chromogranin A (CgA). PA-018 was negative for NSE and CgA as compared to the islet cells in normal human pancreas  (Fig. 1c, panels viii–ix).
Evaluation of different chemotherapies demonstrate that oxaliplatin sustains tumor growth inhibition in the PACC PDTX mouse model
Schedules and dosing represent a blend of the maximally effective dose and/or maximally tolerated dose based on several experiments conducted by Charles River Discovery. Doses are in a range that reflects preclinical studies that have been seen previously in the literature [25–33].
Result summary of PA-018 therapeutic responses and toxicities
−0.1 % day 14
qwk × 3
−5.9 % day 21
qwk × 3
−7.9 % day 21
qwk × 3
−9.8 % day 21
q3d × 4
−5.2 % Day 39
biwk × 5
−4.3 % day 21
qd × 15
−15.3 % day 14
qwk × 3
−5.1 % day 42
qd × 28
−0.8 % day 21
Plotting both mean and median tumor volume for each treatment group revealed that imatinib had no effect on tumor growth with a TTE of 33.3 days as compared to placebo with a TTE of 32.7 days. T-C was only 0.6, which is the “difference between the median TTE of treated group vs. placebo control”. Irinotecan (T-C = 8.1), gemcitabine (T-C = 5.1), and 5-FU (T-C = 4.6) maintained a strong response while on therapy, but the tumor rapidly grew once the drug was discontinued. Erlotinib (T-C = 21.7), bevacizumab (T-C = 36.1) and liposomal doxorubicin (T-C = 33.1) maintained an intermediate response during therapy and tumor volume slowly increased over time. By far, the most effective agent was oxaliplatin which sustained a strong anti-tumor response for the entire course of this study (74 days) with only 3 weekly treatments (Fig. 2; Table 1). Statistical significance was evaluated by log rank test and overall both oxaliplatin (P = 0.0001) and bevacizumab (P = 0.0413) treatments demonstrated significant anti-tumor activity while liposomal doxorubicin was considered trending (P = 0.112). Erlotinib was also considered trending, but the data was not evaluable due to nadir from toxicities with body weight (BW) percent change of −15.3 % and 2 TR deaths (Table 1).
Serum lipase levels correlate with response to chemotherapy
Endpoint therapeutic evaluation of proliferation, tumor vascularity, and apoptosis shows that oxaliplatin induces cell death in PACC
Oxaliplatin induces change in PACC morphology and re-expression of digestive enzymes
Gene analysis together with immunofluorescence (IF) and IHC indicates that the PACC patient and PA-018 PDTX model have a BRCA2 mutation
The rarity of pancreatic acinar cell carcinoma (PACC) has contributed to the lack of an effective standard treatment for this deadly disease. One way to understand a disease and test potential treatments is to have an in vivo laboratory model. To date, only a few recorded studies have been able to recapitulate PACC in mice. The oldest PACC model was formed by creating a transgenic mouse that expresses the transforming gene (T-antigen) of the SV40 virus under the control of elastase I, a pancreas specific promoter . This model had been modified to express luciferase to record tumor burden and can be used to test chemotherapeutics . Recently, another mouse model had been made by deleting a gene that blocked mTOR signaling . The importance of mTOR was corroborated by the decreased tumor burden in both of these PACC mouse models when treated with rapamycin [2, 37]. While genetically engineered mouse models (GEMM)s was one approach to a successful model system, the generation of PDTXs hold unique benefits as well.
Within cancer, tumor heterogeneity exists and there may be multiple ways the same kind of cancer can arise [38, 39]. By creating PDTX models we can learn the similarities and differences between tumors that arose from different patients. We are also able to test tumors that have become chemoresistant to specific compounds and uncover possible alternatives [40, 41]. Thus, PDTXs provide representative signaling involved in disease progression and therapeutic responses that recapitulate those seen in patients [39, 42–47]. This is a report of the first fully characterized humanized model of PACC. Our in vivo model of human PACC can provide a novel means to further investigate the genetic and cellular mechanisms of this disease. Hence, we confirmed that the PDTX tumors were composed of human acinar cell carcinoma cells via STR profile, corroboration of biomarker expression seen in PACC patient tissues [15–18] and secretion of lipase into the bloodstream [14, 48] (Additional file 1: Table S1; Figs. 1, 3). With this data, we concluded that the PDTX tumor model (PA-018) was representative of our patient’s PACC, which provided the rationale for us to test multiple monotherapies based upon the patient’s past treatment history as well as gene array and protein expression data  (Fig. 2).
Imatinib showed little response in PA-018, this mimicked the mixed and only transient clinical response to imatinib in the patient . Liposomal doxorubicin (Doxil) led to significant tumor shrinkage of multiple tumors in the liver of the patient, with a decrease in serum lipase and improvement in his quality of life . Treatment was stopped due to known cardiac toxicity of doxorubicin and the patient’s cancer progressed. Similarly, the PDTX model demonstrated growth inhibition to liposomal doxorubicin but the overall outcome was not significant (P = 0.112) due to the tumor growing back after treatment ended (Fig. 2; Table 1). Bevacizumab, an angiogenesis inhibitor was briefly administered after the patient’s metastatic disease had progressed but due to symptoms of confusion, the therapy was discontinued before the efficacy of the drug could have been assessed. In this study, we saw a statistically significant time to end point tumor volume (TTE) of bevacizumab treated mice (P = 0.0413) (Fig. 2; Table 1). These data leaves open the possibility of bevacizumab as an effective chemotherapy.
We aimed to test standard treatments along with new rationally designed monotherapies. 5-FU and gemcitabine have both been clinically recommended chemotherapeutics for pancreatic cancer  and were added to this study to compare with drugs administered to the PA-018 patient. These two treatments were unable to maintain tumor reduction and were only better than imatinib (Fig. 2; Table 1). From our previous PACC study, we measured RNA levels of the PA-018 tumor biopsy and saw 2.5 fold elevated increase in TOPO1 which can be targeted by irinotecan . As expected, irinotecan demonstrated a strong response while on therapy in PA-018, but off therapy the tumor continued to grow. EGFR expression was also elevated, so erlotinib was tested . There were too many treatment related deaths to truly evaluate this data group. A lower dose of the drug may have to be considered in future exams. The most significant response in the PDTX model was observed following oxaliplatin treatment, which prevented tumor growth weeks after treatment cessation, leading to a durable response (Fig. 2; Table 1). Indeed, oxaliplatin was the only compound to significantly induce apoptosis in the PACC model (Fig. 4).
Like other platinum compounds, oxaliplatin inhibits DNA synthesis by forming both inter- and intra-strand cross links in DNA which prevent DNA replication and transcription, causing cell death . Oxaliplatin is used for treatment of colorectal cancer when it is administered in combination with folinic acid and 5-fluorouracil, (FOLFOX) . In addition, the standard of care for advanced pancreatic cancer is currently FOLFIRINOX (5-FU/folinic acid, irinotecan, and oxaliplatin) . We pursued the beneficial effects of oxaliplatin treatment and observed that the PACC tissue retained amylase and lipase in the cytoplasm and stopped releasing lipase into the blood stream (Figs. 3b, 5). Serum lipase levels can be monitored in the blood as a measure of tumor burden with the expectation that the higher the value, the larger the tumor burden . Serum samples from oxaliplatin treated mice were significantly decreased at day 15 of treatment as compared to the placebo controls (Fig. 3b). These data showed that it is not lipase production but lipase secretion that is indicative of disease, since normal pancreas accumulates lipase in the cytoplasm (Fig. 5a, panel iii) but barely secretes it . By the end of the study, tissue morphology of oxaliplatin treated mice showed that the cancer cells became larger and resembled normal acinar cells more than PACC tissue (Fig. 5a panel i). Indeed, platin drugs have been reported to induce differentiation in cancer cells both in vitro and in vivo [53, 54]. Our data indicates that oxaliplatin inhibits PACC tumor growth by inducing re-differentiation and promoting apoptosis (Figs. 4c, d, 5). Next we assessed why our PACC PDTX model responded well to oxaliplatin.
Previous literature has shown that the presence of BRCA mutations (mutations in a gene involved in DNA repair) in both breast and ovarian cancers are associated with increased sensitivity to platinum compounds [55–57]. This sensitivity has also been shown in ovarian tumors with either germline or sporadic somatic mutations . Furthermore, within the small subset of PDAC patients carrying germline BRCA gene mutations, those treated with platinum compounds had an increase in overall survival . These findings are of particular interest because genomic profiling on 44 PACC patient tumors uncovered DNA repair mutations in 45 % of the samples . In our PDTX model, BRCA1 was mostly localized in the nucleus, while BRCA2 was mostly localized in the cytoplasm and absent from the nucleus, which suggests that the protein is truncated and lost its nuclear localization sequence [59, 60] (Fig. 6a; Additional file 3: Figure S2). The BRCA2 truncation prevents the cell from entering the nucleus and taking part in the DNA homologous recombination repair pathway . Mutational gene analysis of BRCA2 confirms that a 5 base pair deletion on exon 10 (c.1755_1759del5) was present in our PDX model (Fig. 6b). BRCA2 deletions have frequently been associated with deleterious protein truncations [61, 62]. The same gene deletion (referred by the Breast Cancer Information Core as 1983del5) has even been reported as a familial BRCA2 mutation . Using this evidence, we concluded that a BRCA2 truncation mutation may be one potential reason our PACC model was sensitive to oxaliplatin induced cell death. Indeed, previous literature has reported case of a PACC patient with a hereditary BRCA2 mutation. The individual was on a combination of gemcitabine and oxaliplatin, which prolonged their survival, several years longer than the average life expectancy of PACC patients . Overall, previous findings together with our data suggest that oxaliplatin may be beneficial to PACC patients whose tumors carry DNA repair mutations, such as BRCA2.
In summary, it was supported that this newly characterized PACC PDTX model first recapitulated the monotherapy response seen in its matching patient tissue and in the process secondly discovered that oxaliplatin was very effective as a monotherapy in this PACC model. Because of the rarity of PACC, only a handful of retrospective articles and case reports describing experimental therapy have been published. Our findings involving oxaliplatin in this PDTX model warrant further evaluation for the management of pancreatic acinar cell carcinoma. As the first and only preclinical human PACC derived model, the scientific community can use it to better understand the pathobiology of this disease.
pancreatic acinar cell carcinoma
patient derived tumor xenograft
B cell lymphoma/leukemia 10
5 % dextrose in water
tumor growth delay
maximum tolerated dose
neuron specific enolase
short tandem repeat
folinic acid and 5-fluorouracil
5-FU/folinic acid, irinotecan, and oxaliplatin
JCH and LAM were responsible for conception, experimental design, carrying out experiments, and manuscript preparation. ACM was responsible for conception and experimental design of animal experiments. LKD and WFD were responsible for experimental design of animal experiments and carrying out experiments. KAM performed statistical analyses of the animal data. RJM and AJS were responsible for conception and experimental design of animal experiments. DLS was responsible for conception, experimental design of animal experiments, manuscript preparation. MK provided pathology tissue and analysis. DvH contributed concept design and manuscript preparation. JS conducted exome sequence on the PDTX model. SNH and FJC provided mutational analyses. GCO contributed to concept design, manuscript preparation. JAC contributed to concept design, experimental design, and manuscript preparation. All authors read and approved the final manuscript.
We thank Brandy Edenfield for her expertise in histology sample preparation.
Daniel Small, William Durham, Robert Mullin, Louis Dawson, and Julia Schüler are employees of Charles Rivers Discovery Services. Adam Mathias was an employee of Charles Rivers Discovery Services and is now at Delta Synthetic Co. Ltd. Gerardo Colon-Otero receives research support from Novartis. John A. Copland and Laura A. Marlow received royalties from PDTX models licensed to Charles River Discovery Services.
This work was funded in part from NIH/NCI Grant R01CA136665 (JA Copland) and a generous gift from a Grant for rare cancers from Dr. Ellis and Dona Brunton (JA Copland and G Colon-Otero).
Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.
- Holen KD, Klimstra DS, Hummer A, Gonen M, Conlon K, Brennan M, Saltz LB. Clinical characteristics and outcomes from an institutional series of acinar cell carcinoma of the pancreas and related tumors. J Clin Oncol. 2002;20:4673–8.View ArticlePubMedGoogle Scholar
- Zhang N, Lyons S, Lim E, Lassota P. A spontaneous acinar cell carcinoma model for monitoring progression of pancreatic lesions and response to treatment through noninvasive bioluminescence imaging. Clin Cancer Res. 2009;15:4915–24.View ArticlePubMedGoogle Scholar
- Furukawa T, Sakamoto H, Takeuchi S, Ameri M, Kuboki Y, Yamamoto T, Hatori T, Yamamoto M, Sugiyama M, Ohike N, et al. Whole exome sequencing reveals recurrent mutations in BRCA2 and FAT genes in acinar cell carcinomas of the pancreas. Sci Rep. 2015;5:8829.View ArticlePubMedPubMed CentralGoogle Scholar
- Schmidt CM, Matos JM, Bentrem DJ, Talamonti MS, Lillemoe KD, Bilimoria KY. Acinar cell carcinoma of the pancreas in the United States: prognostic factors and comparison to ductal adenocarcinoma. J Gastrointest Surg. 2008;12:2078–86.View ArticlePubMedGoogle Scholar
- Wisnoski NC, Townsend CM Jr, Nealon WH, Freeman JL, Riall TS. 672 patients with acinar cell carcinoma of the pancreas: a population-based comparison to pancreatic adenocarcinoma. Surgery. 2008;144:141–8.View ArticlePubMedGoogle Scholar
- Lowery MA, Klimstra DS, Shia J, Yu KH, Allen PJ, Brennan MF, O’Reilly EM. Acinar cell carcinoma of the pancreas: new genetic and treatment insights into a rare malignancy. Oncologist. 2011;16:1714–20.View ArticlePubMedPubMed CentralGoogle Scholar
- Wood LD, Klimstra DS. Pathology and genetics of pancreatic neoplasms with acinar differentiation. Semin Diagn Pathol. 2014;31:491–7.View ArticlePubMedPubMed CentralGoogle Scholar
- Thomas PC, Nash GF, Aldridge MC. Pancreatic acinar cell carcinoma presenting as acute pancreatitis. HPB (Oxford). 2003;5:111–3.View ArticleGoogle Scholar
- Kuerer H, Shim H, Pertsemlidis D, Unger P. Functioning pancreatic acinar cell carcinoma: immunohistochemical and ultrastructural analyses. Am J Clin Oncol. 1997;20:101–7.View ArticlePubMedGoogle Scholar
- Martin SK, Agarwal G, Lynch GR. Subcutaneous fat necrosis as the presenting feature of a pancreatic carcinoma: the challenge of differentiating endocrine and acinar pancreatic neoplasms. Pancreas. 2009;38:219–22.View ArticlePubMedGoogle Scholar
- Ohno Y, Le Pavoux A, Saeki H, Asahina A, Tamaki K. A case of subcutaneous nodular fat necrosis with lipase-secreting acinar cell carcinoma. Int J Dermatol. 2003;42:384–5.View ArticlePubMedGoogle Scholar
- Zheng ZJ, Gong J, Xiang GM, Mai G, Liu XB. Pancreatic panniculitis associated with acinar cell carcinoma of the pancreas: a case report. Ann Dermatol. 2011;23:225–8.View ArticlePubMedPubMed CentralGoogle Scholar
- Hall JC, Crawford HC. The conspiracy of autophagy, stress and inflammation in acute pancreatitis. Curr Opin Gastroenterol. 2014;30:495–9.View ArticlePubMedPubMed CentralGoogle Scholar
- Klimstra DS, Heffess CS, Oertel JE, Rosai J. Acinar cell carcinoma of the pancreas. A clinicopathologic study of 28 cases. Am J Surg Pathol. 1992;16:815–37.View ArticlePubMedGoogle Scholar
- Horie Y, Gomyoda M, Kishimoto Y, Ueki J, Ikeda F, Murawaki Y, Kawamura M, Hirayama C. Plasma carcinoembryonic antigen and acinar cell carcinoma of the pancreas. Cancer. 1984;53:1137–42.View ArticlePubMedGoogle Scholar
- Wong HH, Chu P. Immunohistochemical features of the gastrointestinal tract tumors. J Gastrointest Oncol. 2012;3:262–84.PubMedPubMed CentralGoogle Scholar
- Hosoda W, Sasaki E, Murakami Y, Yamao K, Shimizu Y, Yatabe Y. BCL10 as a useful marker for pancreatic acinar cell carcinoma, especially using endoscopic ultrasound cytology specimens. Pathol Int. 2013;63:176–82.View ArticlePubMedGoogle Scholar
- La Rosa S, Franzi F, Marchet S, Finzi G, Clerici M, Vigetti D, Chiaravalli AM, Sessa F, Capella C. The monoclonal anti-BCL10 antibody (clone 331.1) is a sensitive and specific marker of pancreatic acinar cell carcinoma and pancreatic metaplasia. Virchows Arch. 2009;454:133–42.View ArticlePubMedGoogle Scholar
- Chmielecki J, Hutchinson KE, Frampton GM, Chalmers ZR, Johnson A, Shi C, Elvin J, Ali SM, Ross JS, Basturk O, et al. Comprehensive genomic profiling of pancreatic acinar cell carcinomas identifies recurrent RAF fusions and frequent inactivation of DNA repair genes. Cancer Discov. 2014;4:1398–405.View ArticlePubMedGoogle Scholar
- Armstrong M, Von Hoff D, Barber B, Marlow L, von Roemeling C, Cooper S, Travis P, Campbell E, Paz-Fumagalli R, Copland J, Colon-Otero G. An effective personalized approach to a rare tumor: prolonged survival in metastatic pancreatic acinar cell carcinoma based on genetic analysis and cell line development. J Cancer. 2011;2:142–52.View ArticlePubMedPubMed CentralGoogle Scholar
- Couch FJ, Hart SN, Sharma P, Toland AE, Wang X, Miron P, Olson JE, Godwin AK, Pankratz VS, Olswold C, et al. Inherited mutations in 17 breast cancer susceptibility genes among a large triple-negative breast cancer cohort unselected for family history of breast cancer. J Clin Oncol. 2015;33:304–11.View ArticlePubMedPubMed CentralGoogle Scholar
- Pin CL, Rukstalis JM, Johnson C, Konieczny SF. The bHLH transcription factor Mist1 is required to maintain exocrine pancreas cell organization and acinar cell identity. J Cell Biol. 2001;155:519–30.View ArticlePubMedPubMed CentralGoogle Scholar
- Jimenez RE, Z’Graggen K, Hartwig W, Graeme-Cook F, Warshaw AL, Fernandez-del Castillo C. Immunohistochemical characterization of pancreatic tumors induced by dimethylbenzanthracene in rats. Am J Pathol. 1999;154:1223–9.View ArticlePubMedPubMed CentralGoogle Scholar
- Notohara K, Hamazaki S, Tsukayama C, Nakamoto S, Kawabata K, Mizobuchi K, Sakamoto K, Okada S. Solid-pseudopapillary tumor of the pancreas: immunohistochemical localization of neuroendocrine markers and CD10. Am J Surg Pathol. 2000;24:1361–71.View ArticlePubMedGoogle Scholar
- Schottle J, Chatterjee S, Volz C, Siobal M, Florin A, Rokitta D, Hinze Y, Dietlein F, Plenker D, Konig K, et al. Intermittent high-dose treatment with erlotinib enhances therapeutic efficacy in EGFR-mutant lung cancer. Oncotarget. 2015;6:38458–68.PubMedPubMed CentralGoogle Scholar
- Shahin M, Soudy R, Aliabadi HM, Kneteman N, Kaur K, Lavasanifar A. Engineered breast tumor targeting peptide ligand modified liposomal doxorubicin and the effect of peptide density on anticancer activity. Biomaterials. 2013;34:4089–97.View ArticlePubMedGoogle Scholar
- Maj E, Filip-Psurska B, Switalska M, Kutner A, Wietrzyk J. Vitamin D analogs potentiate the antitumor effect of imatinib mesylate in a human A549 lung tumor model. Int J Mol Sci. 2015;16:27191–207.View ArticlePubMedPubMed CentralGoogle Scholar
- Rolff J, Becker M, Merk J, Hoffmann J, Fichtner I. Preclinical study of a combination of erlotinib and bevacizumab in early stages of unselected non-small cell lung cancer patient-derived xenografts. Target Oncol. 2016;15:190.Google Scholar
- Tai CJ, Wang H, Wang CK, Huang MT, Wu CH, Chen RJ, Kuo LJ, Wei PL, Chang YJ, Chang CC et al. Bevacizumab and cetuximab with conventional chemotherapy reduced pancreatic tumor weight in mouse pancreatic cancer xenografts. Clin Exp Med. 2016. doi:10.1007/s10238-016-0409-2 PubMedGoogle Scholar
- Hardman WE, Moyer MP, Cameron IL. Efficacy of treatment of colon, lung and breast human carcinoma xenografts with: doxorubicin, cisplatin, irinotecan or topotecan. Anticancer Res. 1999;19:2269–74.PubMedGoogle Scholar
- Nakajima Y, Nakamura T, Enomoto T, Murata Y. Loss of one allele of the p53 gene in the lens epithelial tumor in transgenic mice suppresses apoptosis induced by a topoisomerase I inhibitor (CPT-11). Cancer Lett. 2002;179:165–73.View ArticlePubMedGoogle Scholar
- Fardell JE, Vardy J, Monds LA, Johnston IN. The long-term impact of oxaliplatin chemotherapy on rodent cognition and peripheral neuropathy. Behav Brain Res. 2015;291:80–8.View ArticlePubMedGoogle Scholar
- Nukatsuka M, Nakagawa F, Takechi T. Efficacy of combination chemotherapy using a novel oral chemotherapeutic agent, TAS-102, with oxaliplatin on human colorectal and gastric cancer xenografts. Anticancer Res. 2015;35:4605–15.PubMedGoogle Scholar
- Armstrong T, Packham G, Murphy LB, Bateman AC, Conti JA, Fine DR, Johnson CD, Benyon RC, Iredale JP. Type I collagen promotes the malignant phenotype of pancreatic ductal adenocarcinoma. Clin Cancer Res. 2004;10:7427–37.View ArticlePubMedGoogle Scholar
- Drifka CR, Tod J, Loeffler AG, Liu Y, Thomas GJ, Eliceiri KW, Kao WJ. Periductal stromal collagen topology of pancreatic ductal adenocarcinoma differs from that of normal and chronic pancreatitis. Mod Pathol. 2015;28:1470–80.View ArticlePubMedGoogle Scholar
- Ornitz DM, Hammer RE, Messing A, Palmiter RD, Brinster RL. Pancreatic neoplasia induced by SV40 T-antigen expression in acinar cells of transgenic mice. Science. 1987;238:188–93.View ArticlePubMedGoogle Scholar
- Ding L, Han L, Li Y, Zhao J, He P, Zhang W. Neurogenin 3-directed cre deletion of Tsc1 gene causes pancreatic acinar carcinoma. Neoplasia. 2014;16:909–17.View ArticlePubMedPubMed CentralGoogle Scholar
- Konstantinopoulos PA, Matulonis UA. Current status and evolution of preclinical drug development models of epithelial ovarian cancer. Front Oncol. 2013;3:296.View ArticlePubMedPubMed CentralGoogle Scholar
- Morton CL, Houghton PJ. Establishment of human tumor xenografts in immunodeficient mice. Nat Protoc. 2007;2:247–50.View ArticlePubMedGoogle Scholar
- Bid HK, Kibler A, Phelps DA, Manap S, Xiao L, Lin J, Capper D, Oswald D, Geier B, DeWire M, et al. Development, characterization, and reversal of acquired resistance to the MEK1 inhibitor selumetinib (AZD6244) in an in vivo model of childhood astrocytoma. Clin Cancer Res. 2013;19:6716–29.View ArticlePubMedPubMed CentralGoogle Scholar
- Gandara DR, Mack PC, Bult C, Li T, Lara PN Jr, Riess JW, Astrow SH, Gandour-Edwards R, Cooke DT, Yoneda KY, et al. Bridging tumor genomics to patient outcomes through an integrated patient-derived xenograft platform. Clin Lung Cancer. 2015;16:165–72.View ArticlePubMedPubMed CentralGoogle Scholar
- Monsma DJ, Cherba DM, Richardson PJ, Vance S, Rangarajan S, Dylewski D, Eugster E, Scott SB, Beuschel NL, Davidson PJ, et al. Using a rhabdomyosarcoma patient-derived xenograft to examine precision medicine approaches and model acquired resistance. Pediatr Blood Cancer. 2014;61:1570–7.View ArticlePubMedGoogle Scholar
- Xu S, Li S, Guo Z, Luo J, Ellis MJ, Ma CX. Combined targeting of mTOR and AKT is an effective strategy for basal-like breast cancer in patient-derived xenograft models. Mol Cancer Ther. 2013;12:1665–75.View ArticlePubMedPubMed CentralGoogle Scholar
- Scott C, Becker MA, Haluska P, Samimi G. Patient-derived xenograft models to improve targeted therapy in epithelial ovarian cancer treatment. Front Oncol. 2013;3:295.View ArticlePubMedPubMed CentralGoogle Scholar
- Li S, Shen D, Shao J, Crowder R, Liu W, Prat A, He X, Liu S, Hoog J, Lu C, et al. Endocrine-therapy-resistant ESR1 variants revealed by genomic characterization of breast-cancer-derived xenografts. Cell Reports. 2013;4:1116–30.View ArticlePubMedGoogle Scholar
- Kopetz S, Lemos R, Powis G. The promise of patient-derived xenografts: the best laid plans of mice and men. Clin Cancer Res. 2012;18:5160–2.View ArticlePubMedPubMed CentralGoogle Scholar
- Bertotti A, Migliardi G, Galimi F, Sassi F, Torti D, Isella C, Cora D, Nicolantonio F, Buscarino M. A molecularly annotated platform of patient-derived xenografts (“Xenopatients”) identifies HER2 as an effective therapeutic target in cetuximab-resistant colorectal cancer. Cancer Discov. 2011;1:508–23.View ArticlePubMedGoogle Scholar
- Tenner S, Baillie J, DeWitt J, Vege SS. American college of gastroenterology guideline: management of acute pancreatitis. Am J Gastroenterol. 2013;108(1400–1415):1416.Google Scholar
- Neoptolemos JP, Cunningham D, Friess H, Bassi C, Stocken DD, Tait DM, Dunn JA, Dervenis C, Lacaine F, Hickey H, et al. Adjuvant therapy in pancreatic cancer: historical and current perspectives. Ann Oncol. 2003;14:675–92.View ArticlePubMedGoogle Scholar
- Apps MG, Choi EH, Wheate NJ. The state-of-play and future of platinum drugs. Endocr Relat Cancer. 2015;22:R219–33.PubMedGoogle Scholar
- Yaffee P, Osipov A, Tan C, Tuli R, Hendifar A. Review of systemic therapies for locally advanced and metastatic rectal cancer. J Gastrointest Oncol. 2015;6:185–200.PubMedPubMed CentralGoogle Scholar
- Heinemann V, Haas M, Boeck S. Systemic treatment of advanced pancreatic cancer. Cancer Treat Rev. 2012;38:843–53.View ArticlePubMedGoogle Scholar
- Mizutani Y, Sato N, Kawauchi A, Nonomura N, Fukushima M, Miki T. Cisplatin-induced in vivo differentiation of human embryonal carcinoma. BJU Int. 2002;89:454–8.View ArticlePubMedGoogle Scholar
- Prabhakaran P, Hassiotou F, Blancafort P, Filgueira L. Cisplatin induces differentiation of breast cancer cells. Front Oncol. 2013;3:134.View ArticlePubMedPubMed CentralGoogle Scholar
- Dann RB, DeLoia JA, Timms KM, Zorn KK, Potter J, Flake DD 2nd, Lanchbury JS, Krivak TC. BRCA1/2 mutations and expression: response to platinum chemotherapy in patients with advanced stage epithelial ovarian cancer. Gynecol Oncol. 2012;125:677–82.View ArticlePubMedGoogle Scholar
- Turner NC, Tutt AN. Platinum chemotherapy for BRCA1-related breast cancer: do we need more evidence? Breast Cancer Res. 2012;14:115.View ArticlePubMedPubMed CentralGoogle Scholar
- Wiedemeyer WR, Beach JA, Karlan BY. Reversing platinum resistance in high-grade serous ovarian carcinoma: targeting BRCA and the homologous recombination system. Front Oncol. 2014;4:34.View ArticlePubMedPubMed CentralGoogle Scholar
- Golan T, Kanji ZS, Epelbaum R, Devaud N, Dagan E, Holter S, Aderka D, Paluch-Shimon S, Kaufman B, Gershoni-Baruch R, et al. Overall survival and clinical characteristics of pancreatic cancer in BRCA mutation carriers. Br J Cancer. 2014;111:1132–8.View ArticlePubMedPubMed CentralGoogle Scholar
- Spain BH, Larson CJ, Shihabuddin LS, Gage FH, Verma IM. Truncated BRCA2 is cytoplasmic: implications for cancer-linked mutations. Proc Natl Acad Sci U S A. 1999;96:13920–5.View ArticlePubMedPubMed CentralGoogle Scholar
- Watson P, Lieberman R, Snyder C, Clark VJ, Lynch HT, Holt JT. Detecting BRCA2 protein truncation in tissue biopsies to identify breast cancers that arise in BRCA2 gene mutation carriers. J Clin Oncol. 2009;27:3894–900.View ArticlePubMedPubMed CentralGoogle Scholar
- Frank TS, Manley SA, Olopade OI, Cummings S, Garber JE, Bernhardt B, Antman K, Russo D, Wood ME, Mullineau L, et al. Sequence analysis of BRCA1 and BRCA2: correlation of mutations with family history and ovarian cancer risk. J Clin Oncol. 1998;16:2417–25.PubMedGoogle Scholar
- Montagna M, Agata S, De Nicolo A, Menin C, Sordi G, Chieco-Bianchi L, D’Andrea E. Identification of BRCA1 and BRCA2 carriers by allele-specific gene expression (AGE) analysis. Int J Cancer. 2002;98:732–6.View ArticlePubMedGoogle Scholar
- Lubinski J, Phelan CM, Ghadirian P, Lynch HT, Garber J, Weber B, Tung N, Horsman D, Isaacs C, Monteiro AN, et al. Cancer variation associated with the position of the mutation in the BRCA2 gene. Fam Cancer. 2004;3:1–10.View ArticlePubMedGoogle Scholar
- Ploquin A, Baldini C, Vuagnat P, Makhloufi S, Desauw C, Hebbar M. Prolonged survival in a patient with a pancreatic acinar cell carcinoma. Case Rep Oncol. 2015;8:447–50.View ArticlePubMedPubMed CentralGoogle Scholar