Fig. 6

Immunofluorescence and genetic analysis indicates a BRCA2 mutation in the PA-018 PAAC model. a Immunofluorescence staining for BRCA1 and BRCA2. Panel i shows BRCA1 (red) expressed throughout the nucleus and cytoplasm in normal, PDAC, and the PAAC PDTX tissue. In panel ii, normal and PDAC tissue show co-localization of nuclear DAPI stain and BRCA2 but the PAAC PDTX tissue, does not have this co-localization, indicating that BRCA2 expression is confined to the cytoplasm. b Gene mutational analysis of BRCA2 confirms the presence a 5 base pair deletion on exon 10