- Open Access
Decreased IL-17 during treatment of sputum smear-positive pulmonary tuberculosis due to increased regulatory T cells and IL-10
- Lichen Xu†1, 2,
- Guangying Cui†2,
- Hongyu Jia2,
- Yunan Zhu3,
- Yulong Ding2,
- Jianing Chen2,
- Chong Lu2,
- Ping Ye2,
- Hainv Gao2,
- Lanjuan Li2,
- Weihang Ma2Email author,
- Jianxin Lyu1Email author and
- Hongyan Diao1, 2Email author
© The Author(s) 2016
- Received: 22 November 2015
- Accepted: 16 May 2016
- Published: 16 June 2016
Tuberculosis (TB) remains a major public health concern worldwide. Previous studies have demonstrated that IL-17 plays an important role in initial immune response and is involved in both immune-mediated protection and pathology following infection with Mycobacterium tuberculosis (MTB). However, the alterations and regulation of plasma IL-17 level during TB treatment remain unclear. Moreover, the cell type responsible for the production of IL-17 in TB patients requires further study.
A total of 20 acid-fast bacilli smear-positive (AFB-positive) pulmonary TB patients and 20 age- and gender-matched healthy volunteers were included in our study. Blood samples were collected in heparinized tubes at the time of diagnosis (AFB-positive group) and 3 weeks after the initiation of therapy, when the sputum smear conversion (AFB-negative group) occurred, followed by symptomatic improvement. IL-17 levels and IL-17-producing cells in PBMCs were detected. Lymphocyte populations in the peripheral blood between the AFB-positive and AFB-negative groups were compared by flow-cytometry. A549 cells, a cell line of alveolar epithelial cells, were applied to determine the extent of the pathological damage mediated by IL-17 following MTB infection. Recombinant human IL-10 was used to investigate the regulation of IL-17 expression after sputum smear conversion in AFB-positive pulmonary TB patients.
Plasma IL-17 level were elevated in patients with sputum AFB-positive pulmonary TB, but substantially decreased after TB treatment and smear conversion. Our data indicate that NKT-like cells might be the main source of IL-17, in addition to conventional T cells in AFB-positive pulmonary TB patients. The secretion of IL-17 may be suppressed by regulatory T (Treg) cells and IL-10 during TB treatment. Moreover, the IL-17 levels were positively correlated to both the C-reactive protein and erythrocyte sedimentation rate. Therefore, IL-17 was capable of alveolar epithelial cell damage following MTB infection.
The increase in the frequency of Treg cells and IL-10 levels was associated with a decrease in IL-17 in patients receiving TB treatment. Thus, IL-10 and Tregs may function to inhibit immune-mediated pathology in TB patients.
- Regulatory T cells
- Pulmonary tuberculosis
Tuberculosis (TB) is a communicable respiratory disease caused by infection with Mycobacterium tuberculosis (MTB), and ranks as the second leading cause of death from the infectious diseases worldwide . In 2013, there were 9 million new cases of TB diagnosed and 1.5 million deaths due to the disease . Adaptive immune responses mediated by CD4+ T cells and CD8+ T cells, and T helper (Th) 1 cytokines characterized by interferon (IFN)-γ production are associated with a good prognosis and play an important role in countering the progression of MTB infection [2–4]. However, Th1 cells (primarily CD4+ cells producing IFN-γ) alone are not capable of controlling the infection [3, 5] and other factors, including Th2 cells, Th17 cells and regulatory T cells (Treg cells), are also involved in the progression of MTB infection.
Interleukin (IL)-17, also known as IL-17A, is a number of the IL-17 family which range from A to F [6, 7]. However, IL-17 is of particular importance as it is the cytokine primarily secreted by Th17 cells [6, 7]. IL-17 production can be efficiently induced from naive CD4+ T cell by the IL-23 or IL-6, independently of TGF-β . Recent studies have shown that IL-17 plays an important role in the initial immune responses and is involved in both immune protection and immune pathology in MTB infection [2, 9, 10]. The Th17-response is also considered to be the leading mechanism of protection of bronchoalveolar tract and its barrier maintenance .
IL-17 producing CD4+ T cells, activated in response to vaccination, has been shown to inhibit bacterial growth in the lung after MTB infection, as well as promote the production of chemokines that recruit and activate neutrophils and IFN-γ producing CD4+ T cells [12–15]. Moreover, IL-17 is essential for the vaccine-induced protection against MTB infection by inducing the localization of the proinflammatory cytokine producing C-X-C motif chemokine receptor 5-positive (CXCR5+) T cells, thereby promoting early macrophage activation and the control of MTB . In contrast, other studies demonstrated that IL-17 played an essential role in granuloma formation in the lung , and was involved in the pathological damage mediated by the initial neutrophil recruitment following MTB infection . To limit this pathological damage, a serial of immune regulatory factors are in place, including regulatory T (Treg) cells and the production of the anti-inflammatory cytokine IL-10 [19, 20]. IL-10 production by Tregs can effectively inhibit not only IFN-γ expression but also the ability of CD4+ T cells and CD8+ T cells to degranulate in response to MTB . However, the dynamic changes and regulation of IL-17 during TB treatment remains unclear. Moreover, the cell type that is the primary source of IL-17 in TB patients has not been identified to date.
Here, we recruited patients with sputum acid-fast bacilli smear-positive (AFB-positive) pulmonary TB, and compared the changes of plasma cytokines before the initiation of anti-TB therapy and after the sputum smear conversion. We found that plasma IL-17 was elevated in the AFB-positive patients, but substantially decreased following TB treatment and smear conversion. Moreover, IL-17 levels were positively correlated to both the C-reactive protein (CRP) and erythrocyte sedimentation rate (ESR). IL-17 also aggravated alveolar epithelial cells damage following MTB infection. Our findings further indicate that NKT-like cells might also be the main source of IL-17, in addition to conventional T cells in TB patients. Moreover, the secretion of IL-17 may be regulated by Treg cells and IL-10 during anti-TB treatment.
Patients and associated procedures
Clinical characteristics of patients with pulmonary tuberculosis
HC (n = 20)
AFB-positive TB patients (n = 20)
45 ± 21.9 (22–76)
55 ± 19.8 (18–86)
HREZa 20/20 (100)
Blood samples were collected in heparinized tubes at the time of diagnosis (AFB-positive group) and 3 weeks after the initiation of therapy when the sputum smear conversion (AFB-negative group) occurred, followed by a symptomatic improvement. CRP and ESR were determined. Plasma was separated from peripheral blood mononuclear cells (PBMCs) at 3000 rpmfor 5 min and was stored at −80 °C until use. PBMCs were isolated by density centrifugation on with Ficoll, according to the manufacturer’s instructions.
Flow cytometric analysis of lymphocyte subtypes
The following monoclonal antibodies were used in the present study: FITC-anti-CD4/PE-anti-CD8/Percp-anti-CD3, FITC-anti-CD4, PE-anti-CD25, APC-anti-CD127, FITC-anti-CD3/PE-anti-CD56 APC-anti-CD3, FITC-anti-CD56 (BD Pharmingen, San Diego, CA, USA), PE-anti-IL-17A and PE-anti-IFN-γ (eBioscience, ST, USA). Cells were stained according to standard procedures by incubating the antibodies at 4 °C for 30 min. Intracellular cytokine staining was performed by fixing and permeabilizing the cells with the Cytofix/Cytoperm Fixation/Permeabilization Kit (BD Pharmingen, CA, USA) according to the manufacturer’s instructions. Flow cytometry was performed on the BD canto II and the data was analyzed using BD Diva software (BD, San Diego, CA, USA).
A549 cells are an alveolar type-II epithelial cell line, that were cultured in DMEM (Gibco, CA, USA), supplemented with 100 units/mL penicillin, 100 μg/mL streptomycin, and 10 % fetal bovine serum (FBS) (Gibco, CA) at 37 °C, 5 % CO2. Cells were seeded in 96-well tissue culture plates, following 60–70 % adherence. 100 ng/mL recombinant IL-17A (rOPN, R&D, MN, USA) and 15 μg/mL pulmonary Mycobacterium bovis bacille Calmette–Guerin (BCG, Shanghai Institute of Biological Products Co., Ltd, China) were added respectively. Cells and supernatants were harvested at 24 h. The supernatants were used to determine the concentration of lactate dehydrogenase (LDH) according to the manufacturer’s instructions (Roche, Mannheim, Germany). An analysis of cellular apoptosis was carried out according to the manufacturer’s instructions (BD Pharmingen, San Diego, CA, USA).
PBMCs from health volunteers were cultured at 37 °C, 5 % CO2 in RPMI 1640 (Gibco, CA, USA), supplemented with 100 units/mL penicillin, 100 μg/mL streptomycin, and 10 % FBS. Cells were incubated with recombinant IL-10 (10 μg/mL, Biolegend, CA, USA) and BCG (15 μg/mL). Golgi inhibitor was added for the last 5 h of the incubation. Cells were harvested at 24 h, and intracellular cytokine analysis of IL-17A production was conducted according to the manufacturer’s instructions.
IL-17A, IL-6, IL-23 and IFN-γ were determined by the Luminex enzyme immunoassay (Luminex, TX, USA) according to the manufacturer’s protocol (Millipore, Boston, MA, USA).
According to the manufacturer’s instructions, the IL-17A and IFN-γ ELISPOT assays were performed using commercially available kits (eBiosciences, USA). A total of 2 × 106 PBMCs were cultured with PMA (25 ng/mL) and ionomycin (1 ng/mL) in complete RPMI 1640 medium at 37 °C for 24 h. After stimulation, the positive cells were enumerated by an ImmunoSpot S5 Macro Analyzer (C.T.L., Shaker Heights, OH, USA), and expressed as the numbers of IL-17A and IFN-γ spot-forming units per well.
ELISA kit for IL-10 (eBioscience, San Diego, CA, USA) was used to determine the concentrations of IL-10 according to the manufacturers’ instructions.
Data are presented by box plot or mean ± SD. The significance of differences between two groups was determined using a non-parametric test. A correlation analysis was performed using the Pearson’s correlation coefficient analysis. A p value of less than 0.05 was considered to be statistical significance. *p < 0.05. **p < 0.01, ***p < 0.001. All analyses were performed using SPSS software.
IL-17 level increased in AFB-positive patients but decreased after smear conversion
To further verify the decrease of IL-17 level after smear conversion, ELISPOT assays were performed. We found that the numbers of IL-17- and IFN-γ-secreting cells were increased in the AFB-positive group compared to healthy controls (Fig. 1e). In contrast, the numbers of IL-17- and IFN-γ-secreting cells were decreased after smear conversion (Fig. 1e).
Comparison of lymphocyte subgroups in the peripheral blood between the AFB-positive and AFB-negative groups
Recently, it was reported that the activated NKT-like (CD3+CD56+) cells were capable of rapidly producing IL-17 . Thus, we further observed the alterations of NKT-like cells and found that the percentage of NKT-like cells in the AFB-positive group was similar with that in the AFB-negative group (Fig. 2d).
NKT-like cells produced significant amounts of IL-17
The decreased percentage of IL-17-producing cells in NKT-like was consistent with the low expression of plasma IL-17 level in TB patients after smear conversion, which suggested that NKT-like cells might also be an important source of IL-17, in addition to conventional T cells in TB patients.
IL-17 level was correlated to both CRP and ESR
IL-17 could aggravate alveolar epithelial cells damage after BCG stimulation in vitro
LDH is a cytosolic enzyme that is released upon cell damage , and could be released by alveolar epithelial cells. To further confirm the damage IL-17 on alveolar epithelial cells, we examined the level of LDH released by alveolar epithelial cells after IL-17 treatment in vitro. The level of LDH released from alveolar epithelial cells was increased after IL-17 treatment. IL-17 combined with BCG could enhance the release of LDH from alveolar epithelial cells (Fig. 5c). These results suggested that IL-17 could induce alveolar epithelial cells damage and aggravate lung injury in MTB infection.
IL-17 secretion might be regulated by Treg cell and IL-10 after MTB infection
To further illustrate the function of IL-10 on IL-17 secretion during TB infection, we determined the frequency of IL-17-producing cells in healthy PBMCs after BCG stimulation combined with recombinant IL-10. We found that the percentages of IL-17-producing cells in NKT-like cells and IL-17-producing cells in T cells were increased after BCG stimulation (Fig. 6e). The administration of IL-10 could decrease the frequencies of IL-17-producing cells in NKT-like cells and IL-17-producing cells in T cells induced by BCG stimulation (Fig. 6e). These data indicated that Treg cells and IL-10 might inhibit the production of IL-17 during anti-TB treatment.
Tuberculosis is still a major public health problem in the world. Though a series of studies have shown that IFN-γ and CD4+ T cells play important roles in controlling both bacterial growth and immunopathology during MTB infection [2–4, 35, 36], the Th1 cells (primarily CD4+ cells producing IFN-γ) alone is not enough to control the infection [3, 5]. While previous studies have shown that IL-17 is associated with both protection and pathology in the context of an MTB infection [2, 9, 10], the changes in IL-17 production following anti-TB treatment remains unknown.
In this study, we found that plasma IL-17 level was increased in AFB-positive patients, but obviously decreased after smear conversion. IL-17 is a proinflammatory cytokine mainly produced by Th17 lymphocytes, which play an immunoregulatory role by producing a unique spectrum of pro-inflammatory cytokines IL-17A, IL-17F, IL-22, IL-26, and IFN-γ . These cytokines could induce the activation and recruitment of neutrophils, macrophages, and Th1 lymphocytes into the site of infection, which contributed to delimitation of the damaged area in the lung tissue, as well as the inhibition of MTB growth . In contrast, other studies demonstrated that IL-17 played an essential role in the formation of granuloma in the lung , and was involved in pathological damage in the lung by initial neutrophil recruitment after MTB infection . In our study, we found that IL-17, especially combined BCG, could induce alveolar epithelial cells apoptosis and LDH release, which indicated that the increase of IL-17 in AFB-positive pulmonary TB patients might induce lung pathological damage.
T cells are considered as an important source of IL-17 [6, 7, 21]. NKT cells are distinct innate immune T cells which play a key role in the pathogenesis of various immunomediated liver diseases [38, 39]. In MTB infection, NKT cells could produce IL-21 to help B cells for the production of immunoglobulins  and participate in the local immune response against MTB through the production of IFN-γ and the secretion of cytolytic molecules . CD3+CD56+ cells are not classical invariant NKT cells, but CD3+CD56+ cells are co-expressing T cell receptor and NK cell receptors. Recently, it was reported that activated NKT-like cells (CD3+CD56+) were capable of rapidly producing IL-17 [22, 42]. Our previous study showed that the percentage of NKT-like cells was significantly decreased after telbivudine therapy in chronic hepatitis B patients, and that a positive correlation between the frequency of NKT-like cells and serum HBV DNA level was observed . In all pulmonary TB patients, irrespective of the clinical form (infiltrative pulmonary TB and disseminated pulmonary TB) and variant of the MTB infection (drug-sensitive pulmonary TB and drug-resistant pulmonary TB), the content of IL-17+ NKT-like cell in the peripheral blood was elevated compared to healthy individuals . In our study, we found that the frequency of IL-17-producing cells in NKT-like cells was significantly decreased in the AFB-negative group in comparison with AFB-positive group. These data were consistent with the changes of plasma IL-17 level after anti-TB treatment, and suggested that NKT-like cells might also be the main source of IL-17, in addition to conventional T cells in TB patients.
Patients with AFB-positive pulmonary TB are highly infectious. Sputum smear conversion is that the growth of acid-fast bacilli in the lung is controlled, which is associated with a reduction in the rate of treatment failure and relapse [43, 44]. In our study, we found that plasma IL-17 level and the frequency of IL-17-producing cells in NKT-like cells were decreased after anti-TB treatment. Both CRP and ESR are considered as prognostic indicators in patients with pulmonary TB [24–26]. In our study, we found that the increase of IL-17 was correlated to both CRP and ESR, and could aggravate alveolar epithelial cells damage after BCG stimulation. These data indicated that the decrease of plasma IL-17 level and IL-17-producing cells in NKT-like cells frequency after anti-TB treatment might be beneficial for patients. However, the regulation of IL-17 alteration during treatment is still unclear.
Recently, some studies have indicated that Treg cells may be an important protective factor in the progression of immune pathological damage by inhibiting Th17 cell response in a series of diseases, such as chronic hepatitis B [28, 29] and autoimmune disorder [30, 31]. Treg cells could produce amounts of IL-10 [32–34]. In MTB infection, Treg cells and IL-10 also could limit the immune pathological damage [19, 20]. In our study, we found that the percentage of Treg cells was remarkably elevated in the AFB-negative group compared to the AFB-positive group. This was in opposition to the trend exhibited by the frequency of IL-17-producing cells in NKT-like cells. Moreover, the plasma IL-17 level had a significantly negative correlation with IL-10 level. Furthermore, IL-10 could decrease the percentages of IL-17-producing cells in NKT-like cells and IL-17-producing cells in T cells induced by BCG. These data indicated that the increase of the frequency of Tregs and IL-10 level following TB treatment could inhibit the expression of IL-17. This effect may function to alleviate the extent of immune injury in TB patients.
In summary, we found that IL-17 was increased in AFB-positive pulmonary TB patients, but obviously substantially decreased after treatment following anti-TB treatment. Additionally, NKT-like cells might be an important source of IL-17, besides in addition to conventional T cells in TB patients. IL-17 could was also demonstrated to induce lung injury and epithelial apoptosis following MTB infection in MTB infection. The secretion of IL-17 may be regulated by Treg cells and IL-10 during anti-TB treatment. These results suggest that IL-17-producing NKT-like cells may play an important role in pulmonary TB patients.
LCX, GYC, WHM and HYD conceived, designed and performed most of the experiments with significant contributions from HYJ, YNZ, JNC, CL, HNG, LJL; HYJ, PY and YLD contributed sample collection, GYC and YNZ analyzed the data. GYC, LCX, WHM and HYD wrote the paper. JXL and HYD revised the paper. All authors read and approved the final manuscript
This study was supported by the National Natural Science Foundation of China (Nos. 81271810, 81571953).
The authors declare that they have no competing interests.
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- WHO.int. Global tuberculosis report 2014 WHO Programmes: World Health Organization; 2014 [updated October cited 2014 October 22]. http://www.who.int/tb/publications/global_report/en/.
- Fan L, Xiao H, Mai G, Su B, Ernst J, Hu Z. Impaired M. tuberculosis antigen-specific IFN-gamma response without IL-17 enhancement in patients with severe cavitary pulmonary tuberculosis. PLoS ONE. 2015;10:e0127087.View ArticlePubMedPubMed CentralGoogle Scholar
- Goldsack L, Kirman JR. Half-truths and selective memory: interferon gamma, CD4(+) T cells and protective memory against tuberculosis. Tuberculosis. 2007;87:465–73.View ArticlePubMedGoogle Scholar
- da Silva MV, Figueiredo AA, Machado JR, Castellano LC, Alexandre PB, Oliveira RF, Faria GE, Pereira SA, Rodrigues DB, Rodrigues V Jr. T cell activation and proinflammatory cytokine production in clinically cured tuberculosis are time-dependent and accompanied by upregulation of IL-10. PLoS ONE. 2013;8:e65492.View ArticlePubMedPubMed CentralGoogle Scholar
- Abhimanyu, Bose M, Komal, Varma-Basil M. Lack of association between IL17A and IL17F polymorphisms and related serum levels in north Indians with tuberculosis. Gene. 2013;529:195–8.View ArticlePubMedGoogle Scholar
- Ouyang W, Kolls JK, Zheng Y. The biological functions of T helper 17 cell effector cytokines in inflammation. Immunity. 2008;28:454–67.View ArticlePubMedPubMed CentralGoogle Scholar
- Diao H, Liu X, Wu Z, Kang L, Cui G, Morimoto J, Denhardt DT, Rittling S, Iwakura Y, Uede T, Li L. Osteopontin regulates interleukin-17 production in hepatitis. Cytokine. 2012;60:129–37.View ArticlePubMedGoogle Scholar
- Ghoreschi K, Laurence A, Yang XP, Tato CM, McGeachy MJ, Konkel JE, Ramos HL, Wei L, Davidson TS, Bouladoux N, Grainger JR, Chen Q, Kanno Y, Watford WT, Sun HW, Eberl G, Shevach EM, Belkaid Y, Cua DJ, Chen W, O’Shea JJ. Generation of pathogenic T(H)17 cells in the absence of TGF-beta signalling. Nature. 2010;467:967–71.View ArticlePubMedPubMed CentralGoogle Scholar
- Wozniak TM, Saunders BM, Ryan AA, Britton WJ. Mycobacterium bovis BCG-specific Th17 cells confer partial protection against Mycobacterium tuberculosis infection in the absence of gamma interferon. Infect Immun. 2010;78:4187–94.View ArticlePubMedPubMed CentralGoogle Scholar
- Umemura M, Yahagi A, Hamada S, Begum MD, Watanabe H, Kawakami K, Suda T, Sudo K, Nakae S, Iwakura Y, Matsuzaki G. IL-17-mediated regulation of innate and acquired immune response against pulmonary Mycobacterium bovis bacille Calmette–Guerin infection. J Immunol. 2007;178:3786–96.View ArticlePubMedGoogle Scholar
- Kononova TE, Urazova OI, Novitskii VV, Churina EG, Kolobovnikova YV, Ignatov MV, Zakharova PA, Pechenova OV. Functional activity of Th-17 lymphocytes in pulmonary tuberculosis. Bull Exp Biol Med. 2014;156:743–5.View ArticlePubMedGoogle Scholar
- Ballester M, Nembrini C, Dhar N, de Titta A, de Piano C, Pasquier M, Simeoni E, van der Vlies AJ, McKinney JD, Hubbell JA, Swartz MA. Nanoparticle conjugation and pulmonary delivery enhance the protective efficacy of Ag85B and CpG against tuberculosis. Vaccine. 2011;29:6959–66.View ArticlePubMedGoogle Scholar
- Khader SA, Bell GK, Pearl JE, Fountain JJ, Rangel-Moreno J, Cilley GE, Shen F, Eaton SM, Gaffen SL, Swain SL, Locksley RM, Haynes L, Randall TD, Cooper AM. IL-23 and IL-17 in the establishment of protective pulmonary CD4+ T cell responses after vaccination and during Mycobacterium tuberculosis challenge. Nat Immunol. 2007;8:369–77.View ArticlePubMedGoogle Scholar
- Nembrini C, Marsland BJ, Kopf M. IL-17-producing T cells in lung immunity and inflammation. J Allergy Clinical Immunol. 2009;123:986–94 (quiz 95-6).View ArticleGoogle Scholar
- Zygmunt BM, Rharbaoui F, Groebe L, Guzman CA. Intranasal immunization promotes th17 immune responses. J Immunol. 2009;183:6933–8.View ArticlePubMedGoogle Scholar
- Gopal R, Rangel-Moreno J, Slight S, Lin Y, Nawar HF, Fallert Junecko BA, Reinhart TA, Kolls J, Randall TD, Connell TD, Khader SA, Interleukin-17-dependent CXCL13 mediates mucosal vaccine-induced immunity against tuberculosis. Mucosal Immunol. 2013;6:972–84.View ArticlePubMedPubMed CentralGoogle Scholar
- Okamoto Yoshida Y, Umemura M, Yahagi A, O’Brien RL, Ikuta K, Kishihara K, Hara H, Nakae S, Iwakura Y, Matsuzaki G. Essential role of IL-17A in the formation of a mycobacterial infection-induced granuloma in the lung. J Immunol. 2010;184:4414–22.View ArticlePubMedGoogle Scholar
- Cruz A, Fraga AG, Fountain JJ, Rangel-Moreno J, Torrado E, Saraiva M, Pereira DR, Randall TD, Pedrosa J, Cooper AM, Castro AG. Pathological role of interleukin 17 in mice subjected to repeated BCG vaccination after infection with Mycobacterium tuberculosis. J Exp Med. 2010;207:1609–16.View ArticlePubMedPubMed CentralGoogle Scholar
- Geffner L, Basile JI, Yokobori N, Sabio YGC, Musella R, Castagnino J, Sasiain MC, de la Barrera S. CD4(+) CD25(high) forkhead box protein 3(+) regulatory T lymphocytes suppress interferon-gamma and CD107 expression in CD4(+) and CD8(+) T cells from tuberculous pleural effusions. Clin Exp Immunol. 2014;175:235–45.View ArticlePubMedPubMed CentralGoogle Scholar
- Guyot-Revol V, Innes JA, Hackforth S, Hinks T, Lalvani A. Regulatory T cells are expanded in blood and disease sites in patients with tuberculosis. Am J Respir Crit Care Med. 2006;173:803–10.View ArticlePubMedGoogle Scholar
- Iwakura Y, Ishigame H, Saijo S, Nakae S. Functional specialization of interleukin-17 family members. Immunity. 2011;34:149–62.View ArticlePubMedGoogle Scholar
- Diao H, He J, Zheng Q, Chen J, Cui G, Wei Y, Ye P, Kohanawa M, Li L. A possible role for NKT-like cells in patients with chronic hepatitis B during telbivudine treatment. Immunol Lett. 2014;160:65–71.View ArticlePubMedGoogle Scholar
- Unsal E, Aksaray S, Koksal D, Sipit T. Potential role of interleukin 6 in reactive thrombocytosis and acute phase response in pulmonary tuberculosis. Postgrad Med J. 2005;81:604–7.View ArticlePubMedPubMed CentralGoogle Scholar
- Furuhashi K, Shirai T, Suda T, Chida K. Inflammatory markers in active pulmonary tuberculosis: association with Th1/Th2 and Tc1/Tc2 balance. Kekkaku. 2012;87:1–7.PubMedGoogle Scholar
- Shafee M, Abbas F, Ashraf M, Alam Mengal M, Kakar N, Ahmad Z, Ali F. Hematological profile and risk factors associated with pulmonary tuberculosis patients in Quetta, Pakistan. Pak J Med Sci. 2014;30:36–40.PubMedPubMed CentralGoogle Scholar
- Zhu Y, Jia H, Chen J, Cui G, Gao H, Wei Y, Lu C, Wang L, Uede T, Diao H. Decreased osteopontin expression as a reliable prognostic indicator of improvement in pulmonary tuberculosis: impact of the level of interferon-x03B3;-inducible protein 10. Cellular Physiol Biochem. 2015;37:1983–96.View ArticleGoogle Scholar
- Cakebread JA, Haitchi HM, Xu Y, Holgate ST, Roberts G, Davies DE. Rhinovirus-16 induced release of IP-10 and IL-8 is augmented by Th2 cytokines in a pediatric bronchial epithelial cell model. PLoS ONE. 2014;9:e94010.View ArticlePubMedPubMed CentralGoogle Scholar
- Li J, Qiu SJ, She WM, Wang FP, Gao H, Li L, Tu CT, Wang JY, Shen XZ, Jiang W. Significance of the balance between regulatory T (Treg) and T helper 17 (Th17) cells during hepatitis B virus related liver fibrosis. PLoS ONE. 2012;7:e39307.View ArticlePubMedPubMed CentralGoogle Scholar
- Zhang JY, Song CH, Shi F, Zhang Z, Fu JL, Wang FS. Decreased ratio of Treg cells to Th17 cells correlates with HBV DNA suppression in chronic hepatitis B patients undergoing entecavir treatment. PLoS ONE. 2010;5:e13869.View ArticlePubMedPubMed CentralGoogle Scholar
- Samson M, Lakomy D, Audia S. Bonnotte B [T(H)17 lymphocytes: induction, phenotype, functions, and implications in human disease and therapy]. La Revue de medecine interne/fondee par la Societe nationale francaise de medecine interne. 2011;32:292–301.PubMedGoogle Scholar
- Katsifis GE, Moutsopoulos NM, Wahl SM. T lymphocytes in Sjogren’s syndrome: contributors to and regulators of pathophysiology. Clin Rev Allergy Immunol. 2007;32:252–64.View ArticlePubMedGoogle Scholar
- Vignali D. How many mechanisms do regulatory T cells need? Eur J Immunol. 2008;38:908–11.View ArticlePubMedGoogle Scholar
- Kumar NP, Moideen K, Banurekha VV, Nair D, Sridhar R, Nutman TB, Babu S. IL-27 and TGFbeta mediated expansion of Th1 and adaptive regulatory T cells expressing IL-10 correlates with bacterial burden and disease severity in pulmonary tuberculosis. Immun Inflamm Dis. 2015;3:289–99.View ArticlePubMedPubMed CentralGoogle Scholar
- Pandiyan P, Zhu J. Origin and functions of pro-inflammatory cytokine producing Foxp3(+) regulatory T cells. Cytokine. 2015;76:13–24.View ArticlePubMedGoogle Scholar
- Cooper AM. Cell-mediated immune responses in tuberculosis. Annu Rev Immunol. 2009;27:393–422.View ArticlePubMedPubMed CentralGoogle Scholar
- Schwander S, Dheda K. Human lung immunity against Mycobacterium tuberculosis: insights into pathogenesis and protection. Am J Respir Crit Care Med. 2011;183:696–707.View ArticlePubMedGoogle Scholar
- Shah K, Lee WW, Lee SH, Kim SH, Kang SW, Craft J, Kang I. Dysregulated balance of Th17 and Th1 cells in systemic lupus erythematosus. Arthritis Res Ther. 2010;12:R53.View ArticlePubMedPubMed CentralGoogle Scholar
- Almishri W, Deans J, Swain MG. Rapid activation and hepatic recruitment of innate-like regulatory B cells after invariant NKT cell stimulation in mice. J Hepatol. 2015;63:943–51.View ArticlePubMedGoogle Scholar
- Chen J, Wei Y, He J, Cui G, Zhu Y, Lu C, Ding Y, Xue R, Bai L, Uede T, Li L, Diao H. Natural killer T cells play a necessary role in modulating of immune-mediated liver injury by gut microbiota. Sci Rep. 2014;4:7259.View ArticlePubMedPubMed CentralGoogle Scholar
- Wu C, Li Z, Fu X, Yu S, Lao S, Yang B. Antigen-specific human NKT cells from tuberculosis patients produce IL-21 to help B cells for the production of immunoglobulins. Oncotarget. 2015;6:28633–45.PubMedPubMed CentralGoogle Scholar
- Li Z, Yang B, Zhang Y, Ma J, Chen X, Lao S, Li B, Wu C. Mycobacterium tuberculosis-specific memory NKT cells in patients with tuberculous pleurisy. J Clin Immunol. 2014;34:979–90.View ArticlePubMedGoogle Scholar
- Cosmi L, De Palma R, Santarlasci V, Maggi L, Capone M, Frosali F, Rodolico G, Querci V, Abbate G, Angeli R, Berrino L, Fambrini M, Caproni M, Tonelli F, Lazzeri E, Parronchi P, Liotta F, Maggi E, Romagnani S, Annunziato F. Human interleukin 17-producing cells originate from a CD161+ CD4+ T cell precursor. J Exp Med. 2008;205:1903–16.View ArticlePubMedPubMed CentralGoogle Scholar
- Pardeshi GS. Time of default in tuberculosis patients on directly observed treatment. J Glob Infect Dis. 2010;2:226–30.View ArticlePubMedPubMed CentralGoogle Scholar
- Shu CC, Wang JT, Lee CH, Wang JY, Lee LN, Yu CJ. Predicting results of mycobacterial culture on sputum smear reversion after anti-tuberculous treatment: a case control study. BMC Infect Dis. 2010;10:48.View ArticlePubMedPubMed CentralGoogle Scholar