- Open Access
Intra-tumoural vessel area estimated by expression of epidermal growth factor-like domain 7 and microRNA-126 in primary tumours and metastases of patients with colorectal cancer: a descriptive study
© Hansen et al.; licensee BioMed Central. 2015
- Received: 17 June 2014
- Accepted: 10 December 2014
- Published: 16 January 2015
Understanding the biological properties of potential drug targets are important. This is especially true for anti-angiogenic therapies in the search for potential biomarkers. The aim of the present descriptive study was to analyse the intra-tumoural expressions of epidermal growth factor-like domain 7 (EGFL7) and microRNA-126 (miRNA-126) in primary tumours from patients with stage II-IV colorectal cancer (CRC) and in paired samples of primary tumours, regional lymph node metastases and distant metastases.
A total of 126 patients were included. Analyses were performed on resections of primary tumours, regional lymph node metastases, and large needle biopsies from distant metastases. EGFL7 was analysed by immunohistochemistry (IHC) and miRNA-126 by in situ hybridization (ISH). Both biomarkers were quantified by image guided analyses to determine the relative fraction estimates of vessel areas (VA).
The intra-tumoural EGFL7 VA was significantly higher in primary tumours from patients with recurrent disease than in patients without relapse in both stage II and III, p = 0.019 and p = 0.001, respectively. The EGFL7 VA was significantly higher and the miRNA-126 VA significantly lower in regional lymph node metastases compared to primary tumours, p = 0.01 and p < 10−6, respectively. Furthermore, the miRNA-126 VA in liver metastases was significantly lower than in the primary tumours, p = 0.02.
The intra-tumoural expression of EGFL7 in early stages of CRC may influence the risk of post-surgical recurrence. Differential expression of miRNA-126 seems more pronounced in disseminated disease, which supports its role as a regulator in the metastatic process.
- Colorectal neoplasms
- Epidermal growth factor like domain 7
Targeting angiogenesis is a well established strategy in the treatment of patients with metastatic colorectal cancer (mCRC) and the clinical benefit is well documented [1,2]. The initial experiences were based on an antibody targeting the vascular endothelial growth factor A (VEGF-A), one of the most important growth factors in the onset of the angiogenic process [3,4]. The clinical benefit, however, of targeting VEGF-A is rather limited and may be restricted to a subgroup of patients, and new strategies for controlling tumour angiogenesis are constantly being evaluated.
One strategy, currently under investigation in clinical trials, is the targeting of another important pro-angiogenic protein, the epidermal growth factor-like domain 7 (EGFL7). The EGFL7 gene was identified around 2003 and the gene product was characterised as a protein restricted to the vascular system and highly vascularised tissues [5-7]. The expression of EGFL7 is up-regulated during pathophysiological angiogenesis , where it is secreted to the extracellular matrix (ECM), and guides the vascular sprouting process . EGFL7 is important for tubulogenesis  and is able to inhibit endothelial cell (EC) adhesion molecules causing blood vessels to become leaky . In human cancers elevated levels of EGFL7, assessed by immunohistochemistry (IHC) as well as qPCR, have been associated with increased risk of metastatic spread in several solid tumours [10-13]. However, the evidence on EGFL7 in colorectal cancer (CRC) is still very sparse [11,14].
Another therapeutic strategy that may become available in the near future is the targeting of microRNA (miRNA). In the context of anti-angiogenic therapy miRNA-126 replacement may constitute an exiting approach. The miRNA-126 is a highly EC specific miRNA [15,16] regulating EC proliferation, migration, and survival by modulating VEGF-A and angiopoietin 1 driven signalling cascades [17-20]. Regulating blood vessel integrity and extravasation of inflammatory cells into the tumour compartment constitute other functions regulated by this miRNA [17,18,21]. MicroRNA-126 is encoded by intron 7 of the EGFL7 gene , and the EGFL7 mRNA is suggested to constitute one of its many targets , adding complexity to the interplay of this angiogenic couple. The clinical evidence on miRNA-126 in CRC argues for a deregulated expression in tumour tissue [23,24] and a possible clinical importance at both early and advanced stages of the disease [11,14,25,26].
We have previously shown that high miRNA-126 expression in primary tumours, determined by in situ hybridization (ISH) analysis, is predictive of response to chemotherapy in mCRC , and that this association is related to miRNA-126 and not the general vascular density in the tumours , indicating that the miRNA-126 related vascularisation of primary tumours is an important predictor of response to therapeutic intervention.
Translational studies in mCRC are often based on analyses of the resected primary tumour. Whether these results can be extrapolated to the metastatic lesions that are targeted by the treatment, is often unknown. Analysing putative biomarkers in paired samples of metastases and primary tumours may thus provide useful biological insight. Furthermore, analysing biomarker expression at earlier disease stages may likewise be of importance, when evaluating the therapeutic potential of the given pathway in an adjuvant setting.
The aim of this descriptive study was to analyse the expression of EGFL7 and miRNA-126 in the primary tumours of stage II-IV CRC and whether this expression changed in paired samples of primary tumours, regional lymph node metastases and distant metastases.
Reporting in this study is in accordance with the BRISQUE criteria .
The study was approved by the Regional Scientific Ethical Committee and the Danish Data Protection Agency (20010078, S-20080104, S-20100005, VF-20060115, 2007-41-0252), and written informed consent was obtained from all patients enrolled in the study.
Available paired bio-specimens consisted of resected primary tumours and regional lymph node metastases, and large needle biopsies from distant metastases as shown in Figure 1. Some of the bio-specimens contained limited tumour tissue allowing only the EGFL7 or miRNA-126 analysis.
All histological samples followed routine formaldehyde fixation and paraffin embedding (FFPE) and were stored and transported at room temperature. The median storage time from sampling to analysis was 5.7 years. All FFPE tissue blocks were processed at the Department of Pathology, Vejle Hospital, Denmark. Tissue sections for EGFL7 IHC and miRNA-126 ISH analyses were cut adjacently from the same FFPE tissue block.
Tissue sections were stained with an anti-EGFL7 antibody as previously described . Tissue sections, 4 μm thick, were mounted on coated slides and dried for half an hour at 60°C. Deparaffinisation was performed in estisol for 10 min at room temperature. Rehydration was performed in graded alcohol solutions (99-70%). Blocking of endogenous peroxidase was achieved by incubation in 3% hydrogen peroxide for 5 min. Antigen demasking was done by heat-induced epitope retrieval in a microwave oven, using a TEG buffer (TRIS 10 mM, EGTA 0.5 mM, Titriplex®-VI) at pH 9 for 10 min at 1000 W and 15 min. at 440 W. Tris-buffered saline (TBS)/Tween pH 7.6 was added for 5 min. after cooling at room temperature. The anti-EGFL7 antibody was a rabbit polyclonal antibody (ab115786, Abcam Cambridge, UK) used in a 1:200 dilution (diluted in Dako REAL Antibody Diluent, S2022) and incubated for 90 min. (room temp.). After washing in TBS/Tween the visualisation was accomplished using Dako’s EnVision + System-HRP (DAB) for Rabbit Primary Antidodies™, K4011 for 30 min. All sections were counterstained with Mayer haematoxylin.
The specificity of the anti-EGFL7 antibody was tested by pre-incubation of the antibody with recombinant EGFL7 protein. 4 μl recombinant EGFL7 (Novus Biologicals H00051162-P01) and 1 μl of the anti-EGFL7 antibody were mixed in 200 μl dilution buffer (Dako EnVision FLEX WASH BUFFER K8007) to give approximately 2.4 μM antigen versus 0.6 μM antibody. The solution was left for 30 min at room temperature followed by 90 min incubation on the tissue sections. This resulted in greatly reduced staining of ECs and abolished staining of smooth muscle cells, adipocytes, tumour cells as well as enteric neuronal cells and weak background staining. These observations suggest that the antibody recognise EGFL7 in EC, and probably also one or more other similar proteins (Additional file 1: Figure S1).
MicroRNA-126 in situ hybridization
The miRNA-126 ISH was performed essentially as previously described [29,30]. In brief, 6-μm thick tissue sections were pre-treated with a proteinase-K solution followed by hybridization with a double 6-carboxyfluorescein (FAM)-labelled Locked Nucleic Acid (LNA) miRCURY probe (LNA™ microRNA detection probe, Exiqon A/S, Denmark). After stringent washes in SSC buffers, the probes were detected with alkaline phosphatise-conjugated sheep anti-FAM Fab fragments (Roche). The 4-nitro-blue tetrazolium (NBT) and 5-brom-4-chloro-3′-Indolyl-phosphate (BCIP) substrate (Roche) were added and samples were incubated leading to a blue precipitate (the ISH signal). Counterstaining was performed with nuclear fast red. All slides were processed in a Tecan Freedom Evo automated hybridization instrument (Tecan, Männedorf, Switzerland)  in series of 48 slides per run.
EGFL7 and microRNA-126 image analyses
Image acquisition and analysis was carried out according to the methodology previously described .
EGFL7: Image analysis was performed using the Visiopharm integrated microscope and software module (Visiopharm, Hørsholm, Denmark). Whole slide images were obtained for each slide using x1.5 objective. The tumour area was encircled in order to obtain random, systematic sampling of x20 images (x282 total magnification on the computer monitor) for quantification of the EGFL7 expression. Two image acquisition configurations were applied depending on whether the samples were whole tumour resections, lymph nodes or large needle biopsies. For tumour resections, up to 25 non-overlapping x20 images per sample were collected. All images were within the tumour tissue compartment. For large needle biopsies, x20 images were collected to cover the whole tissue area. A fraction of the images contained limited tumour tissue compartments. The pixel classifier prepared for the tumour resections generated estimates of the EGFL7 expression that were 11% higher than those obtained from the large needle biopsy specific classifier, probably due to increased area contribution from empty glands associated with smaller biopsies. A factor 1.11 was consequently used to correct for these estimates. To allow direct comparison between the estimates of EGFL7 expression from resection samples and the biopsy samples, all estimates were calculated as [[area of stained ECs]*[1.11]]/[total tissue area detected]. The total tissue area detected also included nuclei and connective tissue, as mean values of x20 images for each patient. Thus, the estimates of EGFL7 expression are presented as vessel area fractions (VA) without a dimension. Strong DAB staining was only seen in EC while faint and sporadic DAB staining was detected in some other cell types that resulted in a minor contribution to the EGFL7 estimate. The threshold of the classifier was thus adjusted to count only the strong DAB signal resulting in an estimate virtually specific for EC expression of EGFL7. The staining of the large needle biopsies resulted in expression of more widespread background levels in a few samples. Images with tumour ulceration and necrotic tissue, staining artefacts, and those with no cancer cells were excluded.
MicroRNA-126: Image acquisition and analysis was achieved using the same principles as described for EGFL7 with a few exceptions. The colour classifier was prepared to determine the area fraction represented by the blue miRNA-126 ISH signal. As for the EGFL7, only the strong ISH signals were included in the quantitative estimates and the mean score of VA fractions from the sampled images, and calculated for each patient as described for EGFL7. The image analysis protocols were performed by one observer (BSN) unaware of the clinical setting.
Median values were compared using the Wilcoxon rank sum test. Fisher’s exact test was used for comparison between categorical parameters, and linear regression analysis was used to investigate the linear association between continuous variables. All statistical calculations were carried out using the NCSS statistical software (NCSS Statistical Software, Kaysville, UT 84037, USA, version 2007). P values < 0.05 were considered significant, and all tests were two-sided.
Clinico-pathologic characteristics according to disease stage and relapse (N = 126)
Stage of disease at time of operation
N = 20 (%)
N = 13 (%)
N = 20 (%)
N = 19 (%)
N = 54 (%)
Malignancy grade a
Low + medium
Vascular invasion a
Neuronal invasion a
EGFL7 VA b
Median (95% CI)
Median (95% CI)
The median estimates of intra-tumoural EGFL7 and miRNA-126 VA in primary tumours according to stage and relapse are shown in Table 1. The median EGFL7 VA was significantly higher in patients with recurrent disease compared to patients with stage II (p = 0.02) and stage III (p = 0.001), without relapse. There was no significant difference in the median EGFL7 VA of the primary tumours between patients with nonrecurring stage II and III, or between patients with recurring stage II or III and stage IV. There were no significant differences in median miRNA-126 VA between the patient groups.
Significant correlations between VA estimates of EGFL7 and miRNA-126 were demonstrated in patients with stage III disease, relapsed or without relapse, and stage IV, respectively (Additional file 2: Figure S2 A-E).
Regional lymph node metastases
Epidermal growth factor-like domain 7 (EGFL7) and microRNA-126 (miRNA-126) expressions in primary tumours and associated lymph node/distant metastases
EGFL7 vessel area
miRNA-126 vessel area
A borderline significant correlation was detected between estimates of EGFL7 and miRNA-126 VA in the regional lymph node metastases (Additional file 2: Figure S2 F). No significant correlations were detected between the VA of EGFL7 and miRNA-126 in primary tumours and regional lymph node metastases, r = 0.15, p = 0.32, and r = 0.17, p = 0.24, respectively.
The median of EGFL7 and miRNA-126 VA estimates according to location of distant metastases are shown in Additional file 3: Table S1. The median miRNA-126 VA in lung metastases was significantly lower than in peritoneal metastases (p = 0.047).
For statistical power, only the VA estimates of EGFL7 and miRNA-126 in the liver metastases were compared with the corresponding expressions in the regional lymph node metastases and the primary tumours. The median miRNA-126 VA was significantly lower in liver metastases than in the primary tumours, p = 0.02 (Table 2). Furthermore, a positive significant correlation between estimates of EGFL7 and miRNA-126 VA was detected in the liver metastases, r = 0.55, p = 0.02 (Additional file 2: Figure S2 G). No significant correlations were found comparing estimates of EGFL7 and miRNA-126 VA in primary tumours and liver metastases, r = −0.26, p = 0.41, and r = −0.01, p = 0.97, respectively.
In the present descriptive study we describe the expression of EC-associated EGFL7 and miRNA-126 by estimating the vessel area fractions in primary tumours of different stages and in paired samples of primary tumours, regional lymph node metastases, and distant metastases from patients with CRC. The results may suggest a possible clinical importance of EGFL7 at early disease stages and a coordinated regulation of this angiogenic couple in localised as well as metastatic disease.
From the methodological point of view one may question whether the obtained VA estimates of EGFL7 and miRNA-126 in the present study are really different from traditional estimates of e.g. microvessel density (MVD) as obtained by CD31 and/or CD34 IHC. However, the MVD estimate is often based on hot-spot sampling, whereas the methodological approach in the present study is based on random, systematic sampling quantifying the intra-tumoural expressions of EGLF7 and miRNA-126 within the whole tumour area. In addition, being area fractions, the VAs do not represent numerical densities, but are relative fraction estimates for the individual tumours. Both CD31 and CD34 play a role in cellular adhesion and angiogenesis, but can often be detected in mesenchymal and bone marrow-derived cells in addition to the ECs, which may contribute to bias in MVD estimates. Our image analysis and sampling approach, based on intensity thresholds, diminished the contribution from mesenchymal cells to the VA estimates. On the other hand, EGLF7 is important for vascular tubulogenesis  and permeability . Moreover, being highly specific for ECs [15,16], miRNA-126 participates in regulating EC proliferation, migration, and survival [17-20], and takes part in adjusting blood vessel integrity and thus the extravasation of inflammatory cells into the tumour compartment [17,18,21]. Thus, a range of functional aspects are associated with EGLF7 and miRNA-126, which are unrelated to that of CD31 and/or CD34. We have previously reported that MVD estimates of CD34- correlate with that of miRNA-126- , but the association between the parameters was weak.
The EGFL7 immunoperoxidase staining showed an intense staining of EC, but also faint signals in other cell populations, including adipocytes, smooth muscle cells, cancer cells and enteric neuronal cells. This is surprising since miRNA-126 is located within the EGFL7 precursor transcript and therefore high concordance in the expression patterns between the two is to be expected. However, it should be noted that expression of EGFL7 has been reported by others in cancer cells [12,13] and neurons . Importantly, however, it was clear that the strongest EGFL7 signal was seen in ECs and the implementation of a signal intensity threshold in the image analysis, and restricting quantification within the tumour area, enabled us to obtain estimates of tumour-derived blood vessel specific EGFL7 expression. The miRNA-126 ISH signal was generally EC specific in both the primary tumours and metastatic lesions, in accordance with our previous findings . Our observation that EGFL7 immunostaining was seen in non-EC cells questions the specificity of the EGFL7 antibody employed. Our antibody specificity test suggested that the antibody specifically reacted with the other cell populations, including adipocytes, smooth muscle cells, and cancer cells. If EGFL7 expression is EC specific, this observation implies that the antibody cross-reacts with one or more similar proteins expressed in those cell populations. On the other hand, if the EGFL7 immunostaining observed in the non-EC compartments represents genuine EGFL7, the different expression patterns between EGFL7 and miRNA-126 suggest differential post-transcriptional processing involving parallel EGFL7 biosynthesis and miRNA-126 degradation, as also discussed further below. Future studies employing more and highly specific anti-EGFL-7 antibodies will be required to address this important question.
Estimates of EGFL7 VA in primary tumours of stages II and III were significantly higher in the case of relapsing disease and primarily disseminated tumours (stage IV), compared to tumours without relapse. These results indicate a possible role of EGFL7 in the metastatic process of CRC cells at an early disease stage. This is in accordance with EGFL7 functioning as a pro-angiogenic protein enhancing tumour growth and potentially also dissemination of tumour cells [10,32,33]. We were unable to identify previous studies analysing the protein expression of EGFL7 in early stage CRC. One study, however, by Díaz et al. analysed the EGFL7 mRNA expression in 110 patients with CRC stage I-IV, and demonstrated a significantly higher EGFL7 mRNA expression in stage III and IV than in stage I and II tumours, which supports the present results . Furthermore, pre-clinical studies have demonstrated a relationship between high EGFL7 expression and increased risk of metastatic spread in hepatocellular, breast, and lung cancer [10,12]. A similar relationship has been demonstrated by analysing EGFL7, using IHC in samples from patients with laryngeal carcinomas .
On the other hand, we found no significant differences in VA estimates of miRNA-126 in the primary tumours according to disease stage or relapse. Previous studies in CRC have shown divergent results indicating prognostic as well as no prognostic impact of miRNA-126 in early disease stages. Moreover, a higher as well as a lower expression of miRNA-126 in CRC tissue compared to normal colonic tissue have been reported [11,23,24,26,34]. There may be multiple explanations such as differences in the methodological approach, sample sizes, tissue used for analyses, quantification methods, normalisation procedures etc., which are all known to influence the final results . Also, the multiple known targets of miRNA-126 may blur the results of the present analysis or perhaps miRNA-126 is more important (as a biomarker) in the metastatic setting than at earlier disease stages in this particular context.
A positive significant correlation was demonstrated between the VA estimates of EGFL7 and miRNA-126 in stage III and IV tumours. Since the miR-126 sequence is located within the EGFL7 mRNA precursor, some degree of correlation in expression levels would be expected, although we are here considering the EGFL7 protein and the miRNA-126 (transcript). Some investigators have found a positive correlation between EGFL7 and miR-126 [16,35-37], others have, interestingly, been unable to detect such an association [11,16], which supports the lack of significant correlation in the present study.
The VA estimates of EGFL7 were significantly higher, and the estimates of miRNA-126 significantly lower, in regional lymph node metastases than in primary tumours. This corresponds rather well with the assumption of high EGFL7 and low miRNA-126 expressions being associated with an increased metastatic potential. The correlation between VA estimates of EGFL7 and miRNA-126 in the lymph node metastases only reached borderline significance, tentatively supporting a positive correlation between the estimates. We were unable to identify previous studies assessing estimates of EGFL7 and miRNA-126 expression in paired samples of primary CRC and lymph node metastases.
In the present study VA estimates of EGFL7 and miRNA-126 were obtainable from all analysed metastatic lesions at different organ sites. Although VA estimates of miRNA-126 differed significantly between lung and peritoneal metastases, further comparisons were restricted to liver metastases for reasons of statistical power. We detected no differences between VA estimates of EGFL7 in the liver metastases and primary tumours or regional lymph node metastases. However, the VA estimates of the miRNA-126 were significantly lower in liver metastases than in primary tumours. This finding is comparable with the difference between primary tumours and regional lymph nodes, suggesting a general down-regulation of the miRNA-126 expression in metastatic lesions. This is consistent with previous reports on breast cancer, where miRNA-126 has been documented to be down-regulated in metastatic tissue compared to primary tumours [36,38,39]. A possible difference, although non-significant, was also detected between VA estimates of miRNA-126 in liver and regional lymph node metastases. These results are in line with the study by Png et al. in which a suppressing role of metastatic initiation and metastatic colonization was demonstrated for miRNA-126 . These effects were mediated through suppression of EC recruitment to the metastatic lesions caused by suppression of the IGFBP2, PITPNCI, and MERTK genes. Together with the results obtained in the primary tumours, these data may indicate that EGLF7 and miRNA-126 exert their angiogenic influences differently, depending on the stage of the disease. In accordance with the above comparisons a positive significant correlation between VA estimates of EGFL7 and miRNA-126 was seen in liver metastases, suggesting a conserved regulation of their common expression at the distant metastatic organ site.
The results argue for a potential role of EGFL7 in the early metastatic process leading to relapse after curatively intended surgery for stage II and III CRC and call for further clinical validation.
A pronounced down-regulation of miRNA-126 in metastatic lesions compared to the primary tumour was detected supporting its role in the metastatic process.
The present results demonstrated a significant correlation between the VA estimates of EGFL7 and miRNA-126 in advanced stages supporting a coordinated regulation of their expression.
The visualisation of EGFL7 suggested an expression pattern with a high EC affinity, however, expression in other cell types is suggested. This observation requires further investigation.
We are very thankful for the technical assistance provided by Birgit Roed Sørensen and Trine Møller and for the linguistic editing provided by Karin Larsen.
This study was supported by The Research Council of Lillebaelt Hospital, The Cancer Foundation, and The Danish Council for Independent Research, none of which had any influence on any part of the study.
The Research Council of Lillebaelt Hospital, The Cancer Foundation, and The Danish Council for Independent Research.
- Cao Y, Tan A, Gao F, Liu L, Liao C, Mo Z. A meta-analysis of randomized controlled trials comparing chemotherapy plus bevacizumab with chemotherapy alone in metastatic colorectal cancer. Int J Colorectal Dis. 2009;24:677–85.PubMedView ArticleGoogle Scholar
- Wagner AD, Arnold D, Grothey AA, Haerting J, Unverzagt S. Anti-angiogenic therapies for metastatic colorectal cancer. Cochrane Database Syst Rev. 2009;3:CD005392. doi:10.1002/14651858.CD005392.PubMedGoogle Scholar
- Ferrara N, Gerber HP, LeCouter J. The biology of VEGF and its receptors. Nat Med. 2003;9:669–76.PubMedView ArticleGoogle Scholar
- Folkman J. What is the evidence that tumors are angiogenesis dependent? J Natl Cancer Inst. 1990;82:4–6.PubMedView ArticleGoogle Scholar
- Fitch MJ, Campagnolo L, Kuhnert F, Stuhlmann H. Egfl7, a novel epidermal growth factor-domain gene expressed in endothelial cells. Dev Dyn. 2004;230:316–24.PubMed CentralPubMedView ArticleGoogle Scholar
- Parker LH, Schmidt M, Jin SW, Gray AM, Beis D, Pham T, et al. The endothelial-cell-derived secreted factor Egfl7 regulates vascular tube formation. Nature. 2004;428:754–8.PubMedView ArticleGoogle Scholar
- Soncin F, Mattot V, Lionneton F, Spruyt N, Lepretre F, Begue A, et al. VE-statin, an endothelial repressor of smooth muscle cell migration. EMBO J. 2003;22:5700–11.PubMed CentralPubMedView ArticleGoogle Scholar
- Campagnolo L, Leahy A, Chitnis S, Koschnick S, Fitch MJ, Fallon JT, et al. EGFL7 is a chemoattractant for endothelial cells and is up-regulated in angiogenesis and arterial injury. Am J Pathol. 2005;167:275–84.PubMed CentralPubMedView ArticleGoogle Scholar
- Schmidt M, Paes K, De MA, Smyczek T, Yang S, Gray A, et al. EGFL7 regulates the collective migration of endothelial cells by restricting their spatial distribution. Development. 2007;134:2913–23.PubMedView ArticleGoogle Scholar
- Delfortrie S, Pinte S, Mattot V, Samson C, Villain G, Caetano B, et al. Egfl7 promotes tumor escape from immunity by repressing endothelial cell activation. Cancer Res. 2011;71:7176–86.PubMedView ArticleGoogle Scholar
- Diaz R, Silva J, Garcia JM, Lorenzo Y, Garcia V, Pena C, et al. Deregulated expression of miR-106a predicts survival in human colon cancer patients. Genes Chromosomes Cancer. 2008;47:794–802.PubMedView ArticleGoogle Scholar
- Wu F, Yang LY, Li YF, Ou DP, Chen DP, Fan C. Novel role for epidermal growth factor-like domain 7 in metastasis of human hepatocellular carcinoma. Hepatology. 2009;50:1839–50.PubMedView ArticleGoogle Scholar
- Li JJ, Yang XM, Wang SH, Tang QL. Prognostic role of epidermal growth factor-like domain 7 protein expression in laryngeal squamous cell carcinoma. J Laryngol Otol. 2011;125:1152–7.PubMedView ArticleGoogle Scholar
- Hansen TF, Christensen R, Andersen RF, Sorensen FB, Johnsson A, Jakobsen A. MicroRNA-126 and epidermal growth factor-like domain 7-an angiogenic couple of importance in metastatic colorectal cancer. Results from the Nordic ACT trial. Br J Cancer. 2013;109:1243–51.PubMed CentralPubMedView ArticleGoogle Scholar
- Wienholds E, Kloosterman WP, Miska E, varez-Saavedra E, Berezikov E, de Bruijn E, et al. MicroRNA expression in zebrafish embryonic development. Science. 2005;309:310–1.PubMedView ArticleGoogle Scholar
- Zhu N, Zhang D, Xie H, Zhou Z, Chen H, Hu T, et al. Endothelial-specific intron-derived miR-126 is down-regulated in human breast cancer and targets both VEGFA and PIK3R2. Mol Cell Biochem. 2011;351:157–64.PubMedView ArticleGoogle Scholar
- Fish JE, Santoro MM, Morton SU, Yu S, Yeh RF, Wythe JD, et al. miR-126 regulates angiogenic signaling and vascular integrity. Dev Cell. 2008;15:272–84.PubMed CentralPubMedView ArticleGoogle Scholar
- Wang S, Aurora AB, Johnson BA, Qi X, McAnally J, Hill JA, et al. The endothelial-specific microRNA miR-126 governs vascular integrity and angiogenesis. Dev Cell. 2008;15:261–71.PubMed CentralPubMedView ArticleGoogle Scholar
- Guo C, Sah JF, Beard L, Willson JK, Markowitz SD, Guda K. The noncoding RNA, miR-126, suppresses the growth of neoplastic cells by targeting phosphatidylinositol 3-kinase signaling and is frequently lost in colon cancers. Genes Chromosomes Cancer. 2008;47:939–46.PubMed CentralPubMedView ArticleGoogle Scholar
- Sessa R, Seano G, di Blasio L, Gagliardi PA, Isella C, Medico E, et al. The miR-126 regulates angiopoietin-1 signaling and vessel maturation by targeting p85beta. Biochim Biophys Acta. 1823;2012:1925–35.Google Scholar
- Harris TA, Yamakuchi M, Ferlito M, Mendell JT, Lowenstein CJ. MicroRNA-126 regulates endothelial expression of vascular cell adhesion molecule 1. Proc Natl Acad Sci U S A. 2008;105:1516–21.PubMed CentralPubMedView ArticleGoogle Scholar
- Sun Y, Bai Y, Zhang F, Wang Y, Guo Y, Guo L. miR-126 inhibits non-small cell lung cancer cells proliferation by targeting EGFL7. Biochem Biophys Res Commun. 2010;391:1483–9.PubMedView ArticleGoogle Scholar
- Li XM, Wang AM, Zhang J, Yi H. Down-regulation of miR-126 expression in colorectal cancer and its clinical significance. Med Oncol. 2011;28:1054–7.PubMedView ArticleGoogle Scholar
- Li X, Zhang G, Luo F, Ruan J, Huang D, Feng D, et al. Identification of aberrantly expressed miRNAs in rectal cancer. Oncol Rep. 2012;28:77–84.PubMedGoogle Scholar
- Hansen TF, Soerensen FB, Lindebjerg J, Jakobsen A. The predictive value of microRNA-126 in relation to first line treatment with capecitabine and oxaliplatin in patients with metastatic colorectal cancer. BMC Cancer. 2012;12:83. doi:10.1186/1471-2407-12-83.PubMed CentralPubMedView ArticleGoogle Scholar
- Schepeler T, Reinert JT, Ostenfeld MS, Christensen LL, Silahtaroglu AN, Dyrskjot L, et al. Diagnostic and prognostic microRNAs in stage II colon cancer. Cancer Res. 2008;68:6416–24.PubMedView ArticleGoogle Scholar
- Hansen TF, Nielsen BS, Jakobsen A, Sorensen FB. Visualising and quantifying angiogenesis in metastatic colorectal cancer: A comparison of methods and their predictive value for chemotherapy response. Cell Oncol. 2013;36:341–50.View ArticleGoogle Scholar
- Moore HM, Kelly AB, Jewell SD, McShane LM, Clark DP, Greenspan R, et al. Biospecimen reporting for improved study quality (BRISQ). Cancer Cytopathol. 2011;119:92–101.PubMedView ArticleGoogle Scholar
- Jorgensen S, Baker A, Moller S, Nielsen BS. Robust one-day in situ hybridization protocol for detection of microRNAs in paraffin samples using LNA probes. Methods. 2010;52:375–81.PubMedView ArticleGoogle Scholar
- Nielsen BS, Jorgensen S, Fog JU, Sokilde R, Christensen IJ, Hansen U, et al. High levels of microRNA-21 in the stroma of colorectal cancers predict short disease-free survival in stage II colon cancer patients. Clin Exp Metastasis. 2011;28:27–38.PubMed CentralPubMedView ArticleGoogle Scholar
- Schmidt MH, Bicker F, Nikolic I, Meister J, Babuke T, Picuric S, et al. Epidermal growth factor-like domain 7 (EGFL7) modulates Notch signalling and affects neural stem cell renewal. Nat Cell Biol. 2009;11:873–80.PubMedView ArticleGoogle Scholar
- Pinte SB, Soncin F. Egfl7 promotes tumor escape from immunity. Oncoimmunology. 2012;1:375–6.PubMed CentralPubMedView ArticleGoogle Scholar
- Nikolic I, Stankovic ND, Bicker F, Meister J, Braun H, Awwad K, et al. EGFL7 ligates alphavbeta3 integrin to enhance vessel formation. Blood. 2013;121:3041–50.PubMedView ArticleGoogle Scholar
- Hansen TF, Andersen CL, Nielsen BS, Spindler KL, Sorensen FB, Lindebjerg J, et al. Elevated microRNA-126 is associated with high vascular endothelial growth factor receptor 2 expression levels and high microvessel density in colorectal cancer. Oncology letters. 2011;2:1101–6.PubMed CentralPubMedGoogle Scholar
- Saito Y, Friedman JM, Chihara Y, Egger G, Chuang JC, Liang G. Epigenetic therapy upregulates the tumor suppressor microRNA-126 and its host gene EGFL7 in human cancer cells. Biochem Biophys Res Commun. 2009;379:726–31.PubMedView ArticleGoogle Scholar
- Zhang Y, Yang P, Sun T, Li D, Xu X, Rui Y, et al. miR-126 and miR-126* repress recruitment of mesenchymal stem cells and inflammatory monocytes to inhibit breast cancer metastasis. Nat Cell Biol. 2013;15:284–94.PubMed CentralPubMedView ArticleGoogle Scholar
- Sasahira T, Kurihara M, Bhawal UK, Ueda N, Shimomoto T, Yamamoto K, et al. Downregulation of miR-126 induces angiogenesis and lymphangiogenesis by activation of VEGF-A in oral cancer. Br J Cancer. 2012;107:700–6.PubMed CentralPubMedView ArticleGoogle Scholar
- Png KJ, Halberg N, Yoshida M, Tavazoie SF. A microRNA regulon that mediates endothelial recruitment and metastasis by cancer cells. Nature. 2011;481:190–4.PubMedView ArticleGoogle Scholar
- Tavazoie SF, Alarcon C, Oskarsson T, Padua D, Wang Q, Bos PD, et al. Endogenous human microRNAs that suppress breast cancer metastasis. Nature. 2008;451:147–52.PubMed CentralPubMedView ArticleGoogle Scholar
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