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Anticitrullinated peptide antibodies (ACPA) in the serum of heavy smokers without arthritis - a differential role of associated pulmonary disease?


An increased risk of RA has been described in smokers, but only in ACPA-positive RA patients. The frequency of ACPA in serum of heavy smokers is not known.


Analyze the serum frequency and levels of ACPA on heavy smoker subjects, with and without chronic obstructive pulmonary disease (COPD), and compare them with a healthy control group.


Serum samples of 110 heavy smokers (39% women, 56.9±10 years) were obtained.

Subjects were selected from a Pneumology Service database and from hospital workers. They were compared with 209 healthy controls who had never smoked (51% women, 41.8±12 years). Both groups were tested for two different antibodies against citrullinated proteins, a commercial anti-CCP2 test and a home-made chimeric fibrin/filaggrin citrullinated synthetic peptide (anti-CFFCP).

Their frequency and levels were compared between groups.


Of the 110 heavy smokers, 54 had COPD and 56 were healthy smokers. None had RA. Mean packs-year were 44.3±36 (53±28 in COPD disease and 36±16 in healthy smokers, p<0.01).

Percentage of positive results and mean serum levels of ACPA in all the study groups are shown in Table 1.

Table 1 Frequency of positive autoantibodies and mean serum levels of antibodies in the study groups.

The prevalence of positive anti-CFFCP was higher in the heavy smoker group than in non-smokers, although the difference was not significant. The highest prevalence of positive anti-CFFCP was seen in patients with COPD (7.4%); the difference was at the limit of statistical significance compared with the control group (2.4%) (OR:3.26 95% CI:0.85-12.6 p=0.07). Heavy smokers with COPD had significantly higher levels of both autoantibodies than non-smokers and smokers without COPD.


The prevalence of ACPA in heavy smokers without RA is low, but seems to be higher in heavy smokers with COPD. Larger studies are necessary to confirm these findings and determine the relationship between ACPA and lung disease.

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Open Access This article is published under license to BioMed Central Ltd. This is an Open Access article is distributed under the terms of the Creative Commons Attribution 2.0 International License (, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Ruiz-Esquide, V., Gómara, M.J., Peinado, V. et al. Anticitrullinated peptide antibodies (ACPA) in the serum of heavy smokers without arthritis - a differential role of associated pulmonary disease?. J Transl Med 8 (Suppl 1), P55 (2010).

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