Skip to main content
  • Poster presentation
  • Open access
  • Published:

Increased expression of A-kinase anchoring proteins in T cells from systemic lupus erythematosus patients


Deficient activation of protein kinase A (PKA) is a characteristic of T cells in systemic lupus erythematosus (SLE). A-kinase Anchoring Proteins (AKAPs) associate to and regulate the activity of PKA [1]. Furthermore, some AKAPs are expressed in T cells and influence their function [2]. Therefore, altered expression and/or function of AKAPs can play a role in the deregulated activity of PKA observed in SLE T cells.


To analyse the expression of different AKAPs in T cells isolated from SLE patients.

Patients and methods

T cells were isolated by negative selection using magnetic beads from SLE patients (n= 12) and healthy controls (HC, n= 12). RNA was purified and levels of AKAP79, AKAP95 and AKAP450 mRNA were quantified by RT-qPCR. Subsequently, the analysis of AKAP79 expression was extended to include a total of 24 SLE patients and19 HC. In addition, AKAP79 protein was detected by Western Blot and quantified with Quantity One software.


Levels of AKAP450 mRNA were comparable in HC and SLE T cells (10.73 +/- 0.73 (HC, n= 12) vs 12.69 +/- 1.09 (SLE, n= 12), P= 0.15). However, T Cells from SLE patients had significantly higher levels of AKAP79 and AKAP95 mRNA than HC (AKAP79: 1.99 +/- 0.29 (HC, n= 19) vs 3.61 +/- 0.72 (SLE, n= 24), P= 0.04; AKAP95: 2.58 +/- 0.18 (HC, n= 12) vs 4.13 +/- 0.31 (SLE, n= 12), P= 0.0005). Analysis of AKAP79 protein levels showed increased levels of AKAP79 in SLE T cells compared to HC T cells (0.38 ± 0.05 (HC) vs 0.75 ± 0.18 (SLE), P= 0.06).


Increased levels of AKAP79 and AKAP95 in T cells from SLE patients can contribute to the deregulation of PKA activity in these cells.


  1. Jarnaess E, Taskén K: Spatiotemporal control of cAMP signalling processes by anchored signalling complexes. Biochem Soc Trans. 2007, 35: 931-7. 10.1042/BST0350931.

    Article  CAS  PubMed  Google Scholar 

  2. Schillace RV, Carr SD: The role of Protein Kinase A and A Kinase Anchoring proteins in modulating T cell activation: Progress and future directions. Crit Rev Immunol. 2006, 26: 113-31.

    Article  CAS  PubMed  Google Scholar 

Download references

Author information

Authors and Affiliations


Rights and permissions

Open Access This article is published under license to BioMed Central Ltd. This is an Open Access article is distributed under the terms of the Creative Commons Attribution 2.0 International License (, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Reprints and permissions

About this article

Cite this article

Pérez-Lorenzo, M.J., Galindo, M., García-González, A.J. et al. Increased expression of A-kinase anchoring proteins in T cells from systemic lupus erythematosus patients. J Transl Med 8 (Suppl 1), P49 (2010).

Download citation

  • Published:

  • DOI: