Volume 8 Supplement 1

5th European Workshop on Immune-Mediated Inflammatory Diseases

Open Access

The neonatal Fc receptor is expressed by human lymphocytes

  • K van Bilsen1, 2,
  • J Bastiaans2, 3,
  • W A Dik2,
  • E F De Haas2,
  • S G Baarsma,
  • R W Kuipers and
  • P M van Hagen1, 2
Journal of Translational Medicine20108(Suppl 1):P1

https://doi.org/10.1186/1479-5876-8-S1-P1

Published: 25 November 2010

Introduction

The neonatal Fc receptor (FcRn) is an MHC class I like receptor that binds immunoglobulin G (IgG) in acidic environment (mainly endosomes). The FcRn protects IgG from lysosomal degradation, controls its transport between cell layers and extends IgG serum half-life [1]. It has been shown that the FcRn is expressed by many human cells including APCs and monocytes [2]. Thus far FcRn expression by human lymphocytes has not been proven.

Aim

In this study we examined FcRn expression in isolated mononuclear cells derived from freshly harvested PBMCs.

Methods

Freshly isolated PBMCs were obtained from three healthy volunteers and one buffycoat. NK, B, monocyte and CD4+ and CD8+ cell subsets were sorted by flow cytometry. FcRn and beta-2-microglobulin (β2M) mRNA levels were determined by RQ-PCR. FcRn protein expression from PBMCs obtained from a buffycoat was analyzed by Western blot studies.

Results

RQ-PCR revealed expression of FcRn and B2M mRNA in B, CD4+ and CD8+ cells and monocytes in all three RPE cultures. Western blot analysis demonstrated that mRNA expression in PBMCs from a buffycoat co-existed with FcRn protein expression.

Conclusions

Apart from monocytes, human B, CD4+ and CD8+ cells express low levels of FcRn.

Authors’ Affiliations

(1)
Department of Internal Medicine, Erasmus University Medical Center
(2)
Department of Immunology, Erasmus University Medical Center
(3)
Rotterdam Eye Hospital

References

  1. Roopenian DC, Akilesh S: FcRn: the neonatal Fc receptor comes of age. Nat Rev Immunol. 2007, 7 (9): 715-25. 10.1038/nri2155.View ArticlePubMedGoogle Scholar
  2. Zhu X, Meng G, Dickinson BL, Li X, Mizoguchi E, Miao L, Wang Y, Robert C, Wu B, Smith PD, Lencer WI, Blumberg RS: MHC class I-related neonatal Fc receptor for IgG is functionally expressed in monocytes, intestinal macrophages, and dendritic cells. J Immunol. 2001, 166 (5): 3266-76.PubMed CentralView ArticlePubMedGoogle Scholar

Copyright

© van Bilsen et al; licensee BioMed Central Ltd. 2010

This article is published under license to BioMed Central Ltd.

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