Volume 13 Supplement 1

Melanoma Bridge 2014: meeting abstracts

Open Access

Melanoma Inhibitory Activity and regional lymph node status in malignant melanoma patients

  • Angela Sandru1,
  • Silviu Voinea1,
  • Eugenia Panaitescu2,
  • Madalina Bolovan3,
  • Adina Stanciu3,
  • Sabin Cinca3 and
  • Alexandru Blidaru1
Journal of Translational Medicine201513(Suppl 1):P11

https://doi.org/10.1186/1479-5876-13-S1-P11

Published: 15 January 2015

Background

For many cancers, and malignant melanoma (MM) makes no exception, regional lymph node (RLN) status is decisive for subsequent patients’ outcome. But particularly for MM, stage III comprises a very heterogeneous group of patients, from those with microscopic invasion diagnosed by sentinel node (SN) biopsy to those with matted nodes. Correct staging of patients with clinically free RLN requires SN biopsy. Because this is an invasive maneuver, various substitutes were searched. Thus, in literature there are conflicting references to a possible link between Melanoma Inhibitory Activity (MIA) serum concentration and SN status [1, 2]. MIA, a protein secreted by malignant melanocytes into the extracellular space, blocks the melanoma cells attachment to fibronectin and laminin. Thus MIA increases malignant cells mobility and promotes local invasion and metastasis [3]. In this context, we sought a connection between RLN status and MIA serum concentration in our group of patients.

Materials and methods

150 patients with non-metastatic cutaneous MM were treated in our clinic between 2009 and 2013. They were staged according to 2009 AJCC (American Joint Cancer Committee) staging system. 47 patients were assigned to stage III: 37 presented with clinically evident lymphadenopathies and 10 had positive SN. SN biopsy was performed in 61 patients with intermediate thickness MM and no clinical or ultrasound signs of RLN metastasis. MIA was measured preoperatively in all patients. A cut-off value of 9.4 ng/mL was calculated using ROC curve [4]. Patients were divided in four groups according to RLN status (N0/N1/N2/N3).

Results

Mean and median MIA serum concentration progressively increased along with the number of metastatic RLN. The difference between mean and median MIA values in the 4 groups was not accidental, but the consequence of different tumor load (p = 0.001). Only N0 patients had mean and median MIA concentrations less than 9.4 ng/mL, for all the other N categories, values surpassing the threshold. SN identification rate was 100%. The mean and median MIA serum value in SN positive group were higher than in SN negative one, but the differences were not statistically significant (p=0.6191) and in both cases didn`t exceed the upper normal limit.

Conclusion

MIA serum concentration increases with RLN tumor burden. In our study SN status didn`t correlate with MIA value, but the small number of patients prevents us to draw a conclusion.

Declarations

Acknowledgment

This paper was co-financed from the European Social Fund, through the Sectorial Operational Programme Human Resources Development 2007-2013, project number POSDRU/159/1.5/S/138907 "Excellence in scientific interdisciplinary research, doctoral and postdoctoral, in the economic, social and medical fields -EXCELIS", coordinator The Bucharest University of Economic Studies

Authors’ Affiliations

(1)
Department of Surgical Oncology, “Carol Davila” University of Medicine and Pharmacy; “Alexandru Trestioreanu” Oncologic Institute
(2)
Department of Medical Informatics and Biostatistics, “Carol Davila” University of Medicine and Pharmacy
(3)
Department of Carcinogenesis and Molecular Biology, “Alexandru Trestioreanu” Oncologic Institute

References

  1. Vucetić B, Rogan SA, Hrabac P, Hudorović N, Cupić H, Lukinac L, Ledinsky M, Matejcić A, Lovricević I, Zekan M: Biological value of melanoma inhibitory activity serum concentration in patients with primary skin melanoma. Melanoma Res. 2008, 18 (3): 201-207. 10.1097/CMR.0b013e3283021929.View ArticlePubMedGoogle Scholar
  2. Hofmann MA, Schicke B, Fritsch A, Biesold S, Gussmann F, Küchler I, Voit C, Trefzer U: Impact of lymph node metastases on serum level of melanoma inhibitory activity in stage III melanoma patients. J Dermatol. 2011, 38 (9): 880-886.PubMedGoogle Scholar
  3. Bosserhoff A, Mia: UCSD-Nature Molecule Pages. 2006Google Scholar
  4. Sandru A, Panaitescu E, Voinea S, Bolovan M, Stanciu A, Cinca S, Blidaru A: Prognostic Value of Melanoma Inhibitory Activity Protein in Localized Cutaneous Malignant Melanoma. J Skin Cancer. 2014, Article ID 843214Google Scholar

Copyright

© Sandru et al; licensee BioMed Central Ltd. 2015

This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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