- Open Access
Extracellular matrix production in vitro in cartilage tissue engineering
Journal of Translational Medicine volume 12, Article number: 88 (2014)
Cartilage tissue engineering is arising as a technique for the repair of cartilage lesions in clinical applications. However, fibrocartilage formation weakened the mechanical functions of the articular, which compromises the clinical outcomes. Due to the low proliferation ability, dedifferentiation property and low production of cartilage-specific extracellular matrix (ECM) of the chondrocytes, the cartilage synthesis in vitro has been one of the major limitations for obtaining high-quality engineered cartilage constructs. This review discusses cells, biomaterial scaffolds and stimulating factors that can facilitate the cartilage-specific ECM production and accumulation in the in vitro culture system. Special emphasis has been put on the factors that affect the production of ECM macromolecules such as collagen type II and proteoglycans in the review, aiming at providing new strategies to improve the quality of tissue-engineered cartilage.
Articular cartilage lesions, once caused by trauma or pathology, are hard to heal by itself due to the poor capability of chondrocytes to regenerate. The treatment for articular cartilage lesions is versatile and challenging, which develops from initial microfracture, mosaicplsty to the first (periosteum as the cover for the defects) and second (collagen type I/III as the cover for the defects) generation of autologous chondrocyte implantation (ACI) and gradually widely used matrix-assisted autologous chondrocyte implantation (MACI) [1, 2]. The outcome of the treatment improves with the development of the techniques derived from cartilage tissue engineering. However, there are always some cases with fibrocartilage repair instead of hyaline cartilage regeneration, resulting in inferior mechanical functions of the cartilage .
Cartilage is a special avascular and aneural tissue with sparse chondrocytes embedding in the dense extracellular matrix (ECM) and the cartilage ECM macromolecules play a central role in cartilage functionality, primarily in the establishment of the mechanical functions . Collagen type II, proteoglycan and glycosaminoglycan (GAG), as the primary compositions in the ECM, have always been used as the criteria for the identification and evaluation of the chondrogenic capacity of cells and constructs obtained for cartilage tissue engineering. Parameters such as GAG content, cell mobility and water content usually can be reached up to the level of native tissue. However, the quantity of collagen type II (the major molecule for cartilage mechanical functions) is much lower from cells in vitro than that from the native cartilage [5, 6]. Therefore, an obvious obstacle currently is the inadequate production and accumulation of some chondrocyte-specific ECM macromolecules, especially collagen type II in cultured constructs comparing with native cartilage tissue, even though there has been a great progression in regeneration of the cartilage lesions using engineered constructs . As collagen type II to a large extent provides the tissue with mechanical functions, strategies to promote its production and accumulation in engineered tissues need to be stressed.
This review integrates cells, biomaterial scaffolds and stimuli that can facilitate ECM production and accumulation in cartilage tissue engineering (Table 1), with special focus on their effects on collagen type II expression and production. We endeavor to cover the above aspects that can improve the quality and function of engineered constructs, in the hope of promoting new technology to produce high-quality tissue engineered cartilage.
The cell sources employed in cartilage tissue engineering range from chondrocytes, stem cells to gene-modified cells. Several comparative studies revealed that chondrocytes cultured in vitro could produce more collagen type II than that of its stem cell counterparts as the collagen type II concentration was much higher in cultured chondrocytes [7, 8]. Shahin et al. used human chondrocytes for constructing tissue-engineered cartilage and the obtained collage type II concentration was around 8.5% of dry weight tissue. In contrast, in a similar study using human adipose-derived stem cells, the concentration of collagen type II was only 0.22% of dry weight tissue. However, adipose-derived stem cells showed inferior properties in chondrogenesis comparing with synovium-, bone marrow- and periosteum-derived stem cells in both human and rat [9, 10]. Synovium-derived stem cells have been proved to be superior for the production of cartilage matrix including collagen type II and have the greatest ability for chondrogenesis, among the other stem cell sources . As for the comparison between chondrocytes and synovium-derived stem cells, there was a research demonstrating synovium-derived stem cells and BMSCs produced more GAGs and collagen than chondrocytes . However, the chondrocytes used in the research were P2 cells in monolayer culture (primary culture was termed P0 here), of which the collagen type II and aggrecan significantly decreased as shown in other studies and also in our experiments using a rabbit model (Additional file 1, Figure 1) [12, 13]. Therefore, more investigations need to be explored for the verification. Nevertheless, a recent study showed that fibrocartilage-like tissue produced from synovium-derived stem cells was found in the superficial layer of cultured constructs . While in another study comparing human bone marrow stromal cells (HBMSC) and articular chondrocytes, cartilage-like tissue derived from chondrocytes was found in both superficial zone-like and middle zone-like structure. By contrast, relatively thicker fibrous capsules were shown in constructs from HBMSCs . As superficial zone of the cartilage bears maximum load, it needs to be paid special attention to in cartilage tissue engineering. Chondrocyte and stem cells mentioned above have been summarized in Table 2.
Sorted single cell clone and clonal cell strains were also investigated for chondrogenic capacity. Fluorescence sorted chondrocytes with brighter CD49c and CD44 were detected to expression more collagen type II and higher levels of GAG than unsorted cells . Magnetically sorted stromal cells (STRO-1) from human bone marrow mononuclear populations have been used as seed cells with alginate-chitosan encapsulating and were proved to undergo chondrogenesis with increased collagen type II and proteoglycan in micromass culture and also in culture using a perfused bioreactor . Induced pluripotent stem cells (iPSCs) were also used for screening cells with high chondrogenic capacity. Fluorescence sorted mouse fibroblast-derived iPSCs with GFP driven by Col2 promoter/enhancer showed higher levels of collagen type II, aggrecan and GAGs . Therefore, identification of chondrocyte-specific markers and sorting of cells with relatively higher chondrogenic capacity would provide a new means for the seeding cells for cartilage tissue engineering.
Gene-modified cells employed in cartilage tissue engineering have been paid more attention to nowadays with the rapid development of molecular biology and technology, including cells with single gene transduction and co-transduction of two genes or a gene with an shRNA [19–21]. All these methods aim at improving the chondrogenic capacity of cells and the quality of engineered constructs. As for the details of genes and gene-modified cells that can promote ECM synthesis and collagen type II expression, they will be elucidated in the later part of gene therapy.
Dedifferentiation is the major cause of degeneration of chondrocytes in vitro and maintaining chondrocytes phenotype and features is always on focus. Likewise, for stem cells, chondrogenesis is the primary process to chondrocytes and differentiation of stem cells has been broadly investigated. Optimization of culture methods improves characteristics of chondrocytes and facilitates collagen type II and proteoglycan production in vitro. There are various ways for chondrocytes culture and chondrogenesis of stem cells, including using different media, coating proteins and co-culture systems. Different scaffolds and growth factors are also involved, which will be discussed later.
A study specifically designed to optimize the chondrogenic medium using factorial design of experiments suggested standard chondrogenic medium with increased level of TGF-β1 and glucose and decreased level of dexamethasone could promote collagen type II to collagen type I ratio (ColII/ColI) and better maintain the chondrocyte properties . TGF-β1 in serum free medium has been proved to increase collagen type II expression and better maintain human chondrocyte phenotype comparing with medium plus fetal calf serum (FCS) . Moreover, the redifferentiation of serum free medium cultured monolayer chondrocytes in a 3D scaffold was observed with the expression of collagen type II and proteoglycans; while it was not observed for chondrocytes and STRO-1 cells cultured in FCS supplemented medium [17, 23, 24]. One recent study showed chondrocytes cultured in medium supplemented with 20% platelet-rich plasma produced thicker cartilage tissue than cells in medium with 20% FBS , suggesting platelet-rich plasma was more able to maintain chondrocyte properties in vitro.
Different coatings of cell culture surfaces influences chondrocyte culture in vitro. Cells cultured on collagen type I and II coating dishes expressed more collagen type II expression and produced more glycosaminoglycan (GAG) than uncoated control samples [26, 27]. Though not significantly, higher collagen type II expression was observed in chondrocytes on collagen type II coated dishes than collagen type I coated dishes. On the contrary, fibronectin was suggested to promote collagen type II degradation . More specific research came from Shawn et al., who investigated the effects of coating with zone-specific cartilage extracellular matrix molecules on cartilage formation . Cells cultured in monolayer showed different outcomes with pellets culture. While hyaluronic acid (HA) and osteopontin coating increased Col2a1expression in monolayer culture, pellets culture of cells harvested from HA coated dishes showed decreased expression of collagen type II. On the contrary, tenascin C promoted collagen type II expression in pellet culture while inhibited its expression in monolayer culture. Cells on type II collagen coated dishes showed consistent increased expression of Col2a1 with both monolayer culture and pellet culture system . As different coatings and different culture environments synergistically affect the cell behaviors, both need to be taken into considerations in an experimental design. Aside from protein coating, TiO2-coated coverslips were used for human MSCs culture and proved to significantly enhance cell proliferation without weakening the chondrogenic capacity , which would also benefit the ECM production.
Cell co-culture systems have been used in many fields of biomedical sciences. As for cartilage tissue engineering, co-culture of MSCs and chondrocytes provide us with a means to obtain promising cells. After a comparative study of chondrocytes, MSCs and co-culture of chondrocytes and MSCs, the co-cultured cells were suggested to be the most prospective for cartilage tissue engineering . Co-culture of primary chondrocytes with MSCs or embryonic stem cells or iPSCs increased collagen type II and Sox 9 expression and promoted cartilage matrix formation [3, 32, 33]. The optimal ratio of MSCs and chondrocytes in co-cultures for cartilage engineering varied in different studies. Some study revealed that higher ratios of human MSCs to human articular chondrocytes was more beneficial to chondrogenesis as was shown by newly synthesized cartilaginous ECM and collagen type II gene up-regulation . However, another study suggested 63:1 as the appropriate initial ratio of MSCs/chondrocytes for enough stable chondrocyte-like cells and the constructs from co-cultures were more cartilaginous than that from chondrocytes .
Development and optimization of cell culture methods aiming at elevating the chondrogenic capacity of seed cells will improve the cartilage-specific ECM production and finally benefit the cartilage tissue engineering with relatively higher quality of engineered constructs.
There are various scaffolds used for cartilage tissue engineering as a 3D environment facilitates to maintain chondrocyte properties than monolayer culture. The characteristics of a scaffold involve mechanical strength, biocompatibility, biodegradability, porosity and toxicity . The scaffold must be structurally stable, allow cells to infiltrate and growth factors to attach. All these characteristics of scaffolds provide cells with healthy microenvironment, beneficial cell-cell interaction and adequate nutrient exchange in vitro. Thus, the constructs with cells and ECM mimic the cartilage and are more close to the native cartilage tissue than monolayer cells when implanted in the defects. Of so many scaffolds used, we will focus on clinically used scaffolds and those assist to promote ECM production, especially collagen type II production here.
Collagen is a major component in the cartilage matrix and collagen scaffold has long been used for cartilage repair . Bilayer type I/III collagen membrane has been clinically used worldwide for ACI and MACI and collagen type II production and regeneration of hyaline-like cartilage were obtained during the observations [38–40]. Collagen type II scaffold was also investigated for cartilage repair and in vivo study revealed newly formed cartilage structure the same with normal cartilage . Fibrin glue was often limited to use due to its shrinking feature in vivo. However, when it combined with polyurethane, this new composite scaffold improved cell survival ability and increased the expression of collagen type II and also aggrecan . Polyglycolic acid/fibrin (PGA/fibrin) scaffolds used for chondrocyte culture also enhanced the redifferentiation of ovine chondrocytes and the induction of collagen type II and aggrecan after culture. Biomechanical tests further supported this scaffolds for chondrocyte culture with a high tensile strength of 3.6 N/mm2 . Poly(L-lactic acid) (PLLA) and poly(lactic-co-glycolic) acid (PLGA), as materials that have been approved by the US Food and Drug Administration for human clinical uses , have been often used for the research of cartilage tissue engineering. Comparing with PLLA, collagen type II and Arg-Gly-Asp (RGD) peptide modified PLLA/PLGA (50:50), collagen type II modified PLLA/PLGA (50:50) exhibits higher collagen type II expression and shows superiority for chondrogenesis both in vitro and in vivo. Besides, based on type I collagen-PLGA scaffold, improved funnel-like collagen-PLGA hybrid scaffold was shown to be a stronger promoter for cartilage regeneration . PLGA scaffold with hyaluronic acid incorporated substantially promoted collagen synthesis . Moreover, as PLGA microspheres have been successfully used to immobilize DNA, siRNA and growth factors [48, 49]. Sox9 expression plasmid loaded PLGA microspheres have been investigated for chondrogenesis. Human MSCs seeded on Sox9 gene and heparinized TGF-β3 coated dexamethsone loaded PLGA microspheres could drastically increase collagen type II expression by 30 times compared with control . Therefore, gene incorporation provides a new and promising strategy to optimize the property of scaffolds for tissue engineering.
Comparing with the traditional scaffolds, more and more novel scaffolds have been under investigations, including peptide-modified scaffolds and mobile scaffolds. Mesenchymal stem cells cultured in Arg-Gly-Asp-Ser (RGDS) peptide incorporated PEG-based hydrogels had greater gene expression of collagen type II and produced significantly more GAGs comparing with control . A newly designed synthetic link N peptide nanofiber was suggested to remarkably promote collagen type II and aggrecan production . Since cell expansion would lead to contact inhibition and to avoid the dedifferentiation of chondrocytes through passaging, mobile scaffold was also examined for chondrocyte culture. Differently from traditional biomaterial scaffolds, the culture surface area of the high-extension silicone rubber dish could increase by 8 folds with a motorized device . Thus, adequate cells could be obtained and extracellular matrix was simultaneously maintained, including collagen type II, which would drastically decrease through passaging.
ECM-based scaffold is an emerging approach in the field of cartilage tissue engineering in that it may be relatively easy to retain the natural growth factors in the ECM . Cartilage-derived and cell-derived ECM scaffolds have been investigated and cartilage-like tissues have been observed [55–57]. However, the quality of formed cartilaginous tissue needs to be evaluated and compared. With improvement of the ECM-based scaffold, it would probably be a promising type of scaffolds for cartilage tissue engineering.
Growth factors and compounds
Various growth factors have been used for cartilage tissue engineering. Members in TGF-β family have been widely used to induce the chondrogenesis of MSCs and enhance ECM synthesis. TGF-β3 was proved to be more capable to induce chondrogenesis of MSCs and also increase the expression of collagen type II and aggrecan [41, 58]. As for collagen type II production, the effect of TGF-β1 used in different culture systems was controversial. Depending on the design of the experiments, TGF-β1 could either increase or decrease the expression of collagen type II [59–61]. BMP-2 and IGF-1 both impact chondrogenesis and promote collagen type II production. In a study which compared the effects of BMP-2, IGF-1 and their combination, BMP-2 treatment showed superior ability for collagen type II expression than IGF-1 . Synergistical use of BMP-2 and BMP-7 gave better outcome of ECM production . A more recent study showed standard chondrogenic medium plus TGF-β3, BMP-2 and IGF-1 demonstrated stronger capability for MSCs to produce collagen type II comparing with the other three groups including standard medium with TGF-β3 alone, TGF-β3 + BMP-2 and TGF-β3 + IGF-1 . Another growth factor, connective tissue growth factor (CCN2) has also been proved to strongly enhance the cartilaginous ECM production, including collagen type II and was suggested to reduce age-related changes of articular cartilage [64–67].
Besides the growth factors, some compounds are also proved to effectively induce the expression of collagen type II and other ECM proteins. Hydrocortisone has been found to increase the ability of human articular chondrocytes to produce ECM macromolecules and icariin was proved to help the ECM synthesis in rabbit articular chondrocytes [68–70]. Avocado/soybean unsaponifiables was also identified to facilitate aggrecan and collage type II expression in an osteoblast/chondrocyte co-culture system . Additionally, yeast hydrolysate had been verified to be able to stimulate collagen type II expression and inhibit MMP-13 production in chondrocytes, protecting the cartilage from degradation . However, as for the mechanisms and their clinical use in cartilage tissue engineering, further investigations need to be performed.
Gene therapy has been grown rapidly and is widely used in biomedical sciences. Although gene therapy is in its infancy for cartilage engineering, it shows promising to contribute to the cartilage repair. Often, growth factors are been transfected into chondrocytes or MSCs to improve the properties of cells. IGF-1 and TGF-β1 have been successfully transfected into articular chondrocyte and BMSCs separately and both were proved to increase the expression of collagen type II and aggrecan [20, 72]. Meanwhile, functional study further demonstrated IGF-I transfected implants accelerated the regeneration compared with lacZ implants . Furthermore, one recent study investigating co-transduction of different gene combinations which involved any two of genes IGF-1, FGF-2, TGF-β1 and SOX-9 with adenovirus delivering system showed that cells co-transduced with IGF-1 and FGF-2 could significantly express higher aggrecan, collagen type II and other ECM proteins than other combinations .
In addition to delivering growth factors to cells to increase cartilage-specific gene expression, down regulation of some negative factors has also been proved to effectively promote collagen type II and aggrecan expression. MicroRNA-181b (miR-181b) has been identified to be a negative modulator for cartilage development and siRNA targeting miR-181b induced collagen type II expression and significantly reduced degeneration of cartilage . MiR-145 was proved to target Sox9 and suppress the chondrogenesis of mesenchymal stem cells; while anti-miR-145 inhibitor could rescue the expression of Col2a1, aggrecan and relevant chondrogenic marker genes . Besides, down regulation of some pro-inflammatory factors that could induce cartilage destruction, such as IL-1, IL-6, TNF-α, would also help cartilage regeneration [68, 75, 76]. Therefore, inhibition of the negative modulator could be a promising therapeutic strategy for cartilage regeneration. Furthermore, targeting chondrocyte COL1A1 with siRNA could significantly improve chondrocyte phenotype, with increased ratio of COL2A1/COL1A1 and improved cartilage-like matrix formation .
Furthermore, the combination of delivering growth factors and down-regulation of negative modulators has also been investigated recently. Co-transduction of TGF-β3 with lentiviral transduction system and shRNA targeting Col1a1 with adenoviral vectors demonstrated optimal efficacy for collagen type II expression and cartilage formation . As gene therapy is just arising in cartilage tissue engineering, much needs to be explored in this field.
As cartilage resides in a microenvironment with reduced oxygen and various biomechanical stresses, creating a similar environment in vitro may provide a way to maintain the characteristics of chondrocytes. Hypoxia with 5% or even 3% oxygen stimulated collagen type II expression and biosynthesis of hyaline-like cartilage matrix [39, 78, 79]. For dynamic environment, expression of collagen type II and aggrecan were induced after the stimulation of a uniaxial compressive load with 1kPa, 1Hz for 30 min . Intermittent hydrostatic pressure benefit long-term chondrocyte culture and the cartilaginous construct formation . As for tensile strength, abnormal cyclic tensile strain inhibited collagen type II expression; while proper and cyclic tensile strain would promote collagen type II expression [65, 82]. To provide an environment that could enhance nutrient transport and maintain dynamic state for cells, bioreactors have been used for cartilage tissue engineering. Combined with scaffolds as the support for cells, the system facilitates chondrocyte proliferation and ECM accumulation comparing with static culture [83, 84]. Improved bioreactor as wavy-wall bioreactor better increased the cartilage matrix than common dynamic culture using spinner flasks . Perfusion bioreactor was also used to investigate chondrogenesis and chondrocyte growth. It was found that human articular chondrocytes within alginate beads only produced collage type II in a perfused environment. Collage type II was not detected in the static culture condition by contrast . However, one problem caused by dynamic loading was less accumulation of matrix in the scaffold as part of the newly synthesized molecules would disperse into the medium . Besides dynamic environment mentioned above, cryopreservation has a negative effect on the chondrogenic capacity of chondrocytes. Human chondrocytes showed significantly decreased collagen type II expression after cryopreservation . Although new methods and scaffolds are being under investigation for the preservation and revitalization of articular cartilage or chondrocytes [87–89], it is better to use freshly cultured chondrocytes for clinical application if it is possible.
Cartilage tissue engineering mainly consists of seed cells, scaffolds, stimuli and environmental factors. As cartilage is more specific than other tissues with sparse chondrocytes and dense supportive ECM, emphasis should be put on cell proliferation, differentiation and cartilage-specific ECM production. Low proliferation ability and dedifferentiation property of the chondrocytes limit fine cartilaginous construct formation and use, as well as the efficacy of current therapies. Through the selection of cell sources, optimization of cell culture methods and rise of innovative biomaterial scaffolds, tissue engineered constructs with enhanced cartilage-specific ECM production are promising in the near future. New technologies employing comprehensive factors may make the tissue-engineered cartilage successful and lead the clinical approaches from ACI, MACI to the ideal tissue-engineered cartilage implantation (TEC).
Jiang YZ, Zhang SF, Qi YY, Wang LL, Ouyang HW: Cell transplantation for articular cartilage defects: principles of past, present, and future practice. Cell Transplant. 2011, 20: 593-607. 10.3727/096368910X532738.
Kon E, Filardo G, Di Martino A, Marcacci M: ACI and MACI. J Knee Surg. 2012, 25: 17-22.
Chung C, Burdick JA: Engineering cartilage tissue. Adv Drug Deliv Rev. 2008, 60: 243-262. 10.1016/j.addr.2007.08.027.
Huang AH, Farrell MJ, Mauck RL: Mechanics and mechanobiology of mesenchymal stem cell-based engineered cartilage. J Biomech. 2010, 43: 128-136. 10.1016/j.jbiomech.2009.09.018.
Mahmoudifar N, Doran PM: Chondrogenesis and cartilage tissue engineering: the longer road to technology development. Trends Biotechnol. 2012, 30: 166-176. 10.1016/j.tibtech.2011.09.002.
Dunkelman NS, Zimber MP, Lebaron RG, Pavelec R, Kwan M, Purchio AF: Cartilage production by rabbit articular chondrocytes on polyglycolic acid scaffolds in a closed bioreactor system. Biotechnol Bioeng. 1995, 46: 299-305. 10.1002/bit.260460402.
Shahin K, Doran PM: Strategies for enhancing the accumulation and retention of extracellular matrix in tissue-engineered cartilage cultured in bioreactors. PLoS One. 2011, 6: e23119-10.1371/journal.pone.0023119.
Mahmoudifar N, Doran PM: Chondrogenic differentiation of human adipose-derived stem cells in polyglycolic acid mesh scaffolds under dynamic culture conditions. Biomaterials. 2010, 31: 3858-3867. 10.1016/j.biomaterials.2010.01.090.
Sakaguchi Y, Sekiya I, Yagishita K, Muneta T: Comparison of human stem cells derived from various mesenchymal tissues: superiority of synovium as a cell source. Arthritis Rheum. 2005, 52: 2521-2529. 10.1002/art.21212.
Yoshimura H, Muneta T, Nimura A, Yokoyama A, Koga H, Sekiya I: Comparison of rat mesenchymal stem cells derived from bone marrow, synovium, periosteum, adipose tissue, and muscle. Cell Tissue Res. 2007, 327: 449-462. 10.1007/s00441-006-0308-z.
Vinardell T, Sheehy EJ, Buckley CT, Kelly DJ: A comparison of the functionality and in vivo phenotypic stability of cartilaginous tissues engineered from different stem cell sources. Tissue Eng Part A. 2012, 18: 1161-1170. 10.1089/ten.tea.2011.0544.
Benya PD, Padilla SR, Nimni ME: Independent regulation of collagen types by chondrocytes during the loss of differentiated function in culture. Cell. 1978, 15: 1313-1321. 10.1016/0092-8674(78)90056-9.
Hamada T, Sakai T, Hiraiwa H, Nakashima M, Ono Y, Mitsuyama H, Ishiguro N: Surface markers and gene expression to characterize the differentiation of monolayer expanded human articular chondrocytes. J Med Sci. 2013, 75: 101-111.
Ando W, Fujie H, Moriguchi Y, Nansai R, Shimomura K, Hart DA, Yoshikawa H, Nakamura N: Detection of abnormalities in the superficial zone of cartilage repaired using a tissue engineered construct derived from synovial stem cells. Eur Cell Mater. 2012, 24: 292-307.
Saha S, Kirkham J, Wood D, Curran S, Yang XB: Informing future cartilage repair strategies: a comparative study of three different human cell types for cartilage tissue engineering. Cell Tissue Res. 2013, 352: 495-507. 10.1007/s00441-013-1586-x.
Grogan SP, Barbero A, Diaz-Romero J, Cleton-Jansen AM, Soeder S, Whiteside R, Hogendoorn PC, Farhadi J, Aigner T, Martin I, Mainil-Varlet P: Identification of markers to characterize and sort human articular chondrocytes with enhanced in vitro chondrogenic capacity. Arthritis Rheum. 2007, 56: 586-595. 10.1002/art.22408.
Forsey RW, Tare R, Oreffo RO, Chaudhuri JB: Perfusion bioreactor studies of chondrocyte growth in alginate-chitosan capsules. Biotechnol Appl Biochem. 2012, 59: 142-152. 10.1002/bab.1009.
Diekman BO, Christoforou N, Willard VP, Sun H, Sanchez-Adams J, Leong KW, Guilak F: Cartilage tissue engineering using differentiated and purified induced pluripotent stem cells. Proc Natl Acad Sci U S A. 2012, 109: 19172-19177. 10.1073/pnas.1210422109.
Henrotin YE, Deberg MA, Crielaard JM, Piccardi N, Msika P, Sanchez C: Avocado/soybean unsaponifiables prevent the inhibitory effect of osteoarthritic subchondral osteoblasts on aggrecan and type II collagen synthesis by chondrocytes. J Rheumatol. 2006, 33: 1668-1678.
Madry H, Kaul G, Cucchiarini M, Stein U, Zurakowski D, Remberger K, Menger MD, Kohn D, Trippel SB: Enhanced repair of articular cartilage defects in vivo by transplanted chondrocytes overexpressing insulin-like growth factor I (IGF-I). Gene Ther. 2005, 12: 1171-1179. 10.1038/sj.gt.3302515.
Garza-Veloz I, Romero-Diaz VJ, Martinez-Fierro ML, Marino-Martinez IA, Gonzalez-Rodriguez M, Martinez-Rodriguez HG, Espinoza-Juarez MA, Bernal-Garza D, Ortiz-Lopez R, Rojas-Martinez A: Analyses of chondrogenic induction of adipose mesenchymal stem cells by combined co-stimulation mediated by adenoviral gene transfer. Arthritis Res Ther. 2013, 15: R80(81–13)-
Enochson L, Brittberg M, Lindahl A: Optimization of a chondrogenic medium through the use of factorial design of experiments. BioRes Open Access. 2012, 1: 306-313. 10.1089/biores.2012.0277.
Giannoni P, Pagano A, Maggi E, Arbico R, Randazzo N, Grandizio M, Cancedda R, Dozin B: Autologous chondrocyte implantation (ACI) for aged patients: development of the proper cell expansion conditions for possible therapeutic applications. Osteoarthritis Cartilage. 2005, 13: 589-600. 10.1016/j.joca.2005.02.015.
Shao XX, Duncan NA, Lin L, Fu X, Zhang JY, Yu CL: Serum-free media for articular chondrocytes in vitro expansion. Chin Med J. 2013, 126: 2523-2529.
Petrera M, De Croos JN, Iu J, Hurtig M, Kandel RA, Theodoropoulos JS: Supplementation with platelet-rich plasma improves the in vitro formation of tissue-engineered cartilage with enhanced mechanical properties. Arthroscopy. 2013, 29: 1685-1692. 10.1016/j.arthro.2013.07.259.
Barbero A, Grogan SP, Mainil-Varlet P, Martin I: Expansion on specific substrates regulates the phenotype and differentiation capacity of human articular chondrocytes. Cell Biochem. 2006, 98: 1140-1149. 10.1002/jcb.20754.
Rutgers M, Saris DB, Vonk LA, van Rijen MH, Akrum V, Langeveld D, van Boxtel A, Dhert WJ, Creemers LB: Effect of collagen type I or type II on chondrogenesis by cultured human articular chondrocytes. Tissue Eng Part A. 2013, 19: 59-65. 10.1089/ten.tea.2011.0416.
Yasuda T, Poole AR: A fibronectin fragment induces type II collagen degradation by collagenase through an interleukin-1-mediated pathway. Arthritis Rheum. 2002, 46: 138-148. 10.1002/1529-0131(200201)46:1<138::AID-ART10051>3.0.CO;2-K.
Grogan SP, Chen X, Sovani S, Taniguchi N, Colwell CW, Lotz M, D’Lima D: Influence of cartilage extracellular matrix molecules on cell phenotype and neocartilage formation. Tissue Eng Part A. 2013, 20: 264-274.
Kaitainen S, Mahonen AJ, Lappalainen R, Kroger H, Lammi MJ, Qu C: TiO2 coating promotes human mesenchymal stem cell proliferation without the loss of their capacity for chondrogenic differentiation. Biofabrication. 2013, 5: 025009-10.1088/1758-5082/5/2/025009.
Meretoja VV, Dahlin RL, Wright S, Kasper FK, Mikos AG: The effect of hypoxia on the chondrogenic differentiation of co-cultured articular chondrocytes and mesenchymal stem cells in scaffolds. Biomaterials. 2013, 34: 4266-4273. 10.1016/j.biomaterials.2013.02.064.
Vats A, Bielby RC, Tolley N, Dickinson SC, Boccaccini AR, Hollander AP, Bishop AE, Polak JM: Chondrogenic differentiation of human embryonic stem cells: the effect of the micro-environment. Tissue Eng. 2006, 12: 1687-1697. 10.1089/ten.2006.12.1687.
Qu C, Puttonen KA, Lindeberg H, Ruponen M, Hovatta O, Koistinaho J, Lammi MJ: Chondrogenic differentiation of human pluripotent stem cells in chondrocyte co-culture. Int J Biochem Cell Biol. 2013, 45: 1802-1812. 10.1016/j.biocel.2013.05.029.
Mo XT, Guo SC, Xie HQ, Deng L, Zhi W, Xiang Z, Li XQ, Yang ZM: Variations in the ratios of co-cultured mesenchymal stem cells and chondrocytes regulate the expression of cartilaginous and osseous phenotype in alginate constructs. Bone. 2009, 45: 42-51. 10.1016/j.bone.2008.07.240.
Yang YH, Lee AJ, Barabino GA: Coculture-driven mesenchymal stem cell-differentiated articular chondrocyte-like cells support neocartilage development. Stem Cell Transl Med. 2012, 1: 843-854. 10.5966/sctm.2012-0083.
Mafi P, Hindocha S, Mafi R, Khan WS: Evaluation of biological protein-based collagen scaffolds in cartilage and musculoskeletal tissue engineering–a systematic review of the literature. Curr Stem Cell Res Ther. 2012, 7: 302-309. 10.2174/157488812800793045.
Iwasa J, Engebretsen L, Shima Y, Ochi M: Clinical application of scaffolds for cartilage tissue engineering. Knee Surg Sports Traumatol Arthrosc. 2009, 17: 561-577. 10.1007/s00167-008-0663-2.
Zheng MH, Willers C, Kirilak L, Yates P, Xu J, Wood D, Shimmin A: Matrix-induced autologous chondrocyte implantation (MACI): biological and histological assessment. Tissue Eng. 2007, 13: 737-746. 10.1089/ten.2006.0246.
Legendre F, Ollitrault D, Hervieu M, Bauge C, Maneix L, Goux D, Chajra H, Mallein-Gerin F, Boumediene K, Galera P, Demoor M: Enhanced hyaline cartilage matrix synthesis in collagen sponge scaffolds by using siRNA to stabilize chondrocytes phenotype cultured with bone morphogenetic protein-2 under hypoxia. Tissue Eng Part C Methods. 2013, 19: 550-567. 10.1089/ten.tec.2012.0508.
Willers C, Chen J, Wood D, Xu J, Zheng MH: Autologous chondrocyte implantation with collagen bioscaffold for the treatment of osteochondral defects in rabbits. Tissue Eng. 2005, 11: 1065-1076. 10.1089/ten.2005.11.1065.
Chen WC, Yao CL, Wei YH, Chu IM: Evaluating osteochondral defect repair potential of autologous rabbit bone marrow cells on type II collagen scaffold. Cytotechnology. 2011, 63: 13-23. 10.1007/s10616-010-9314-9.
Lee CR, Grad S, Gorna K, Gogolewski S, Goessl A, Alini M: Fibrin-polyurethane composites for articular cartilage tissue engineering: a preliminary analysis. Tissue Eng. 2005, 11: 1562-1573. 10.1089/ten.2005.11.1562.
Endres M, Neumann K, Zhou B, Freymann U, Pretzel D, Stoffel M, Kinne RW, Kaps C: An ovine in vitro model for chondrocyte-based scaffold-assisted cartilage grafts. J Orthop Trauma Res. 2012, 7: 1-14.
Nair LS, Laurencin CT: Biodegradable polymers as biomaterials. Prog Polym Sci. 2007, 32: 762-798. 10.1016/j.progpolymsci.2007.05.017.
Hsu SH, Chang SH, Yen HJ, Whu SW, Tsai CL, Chen DC: Evaluation of biodegradable polyesters modified by type II collagen and Arg-Gly-Asp as tissue engineering scaffolding materials for cartilage regeneration. Artif Organs. 2006, 30: 42-55. 10.1111/j.1525-1594.2006.00179.x.
Lu H, Ko YG, Kawazoe N, Chen G: Culture of bovine articular chondrocytes in funnel-like collagen-PLGA hybrid sponges. Biomed Mater. 2011, 6: 045011-10.1088/1748-6041/6/4/045011.
Yoo HS, Lee EA, Yoon JJ, Park TG: Hyaluronic acid modified biodegradable scaffolds for cartilage tissue engineering. Biomaterials. 2005, 26: 1925-1933. 10.1016/j.biomaterials.2004.06.021.
Shen H, Hu X, Bei J, Wang S: The immobilization of basic fibroblast growth factor on plasma-treated poly(lactide-co-glycolide). Biomaterials. 2008, 29: 2388-2399. 10.1016/j.biomaterials.2008.02.008.
Zhang XQ, Tang H, Hoshi R, De Laporte L, Qiu H, Xu X, Shea LD, Ameer GA: Sustained transgene expression via citric acid-based polyester elastomers. Biomaterials. 2009, 30: 2632-2641. 10.1016/j.biomaterials.2009.01.021.
Park JS, Yang HN, Woo DG, Jeon SY, Park KH: SOX9 gene plus heparinized TGF-beta 3 coated dexamethasone loaded PLGA microspheres for inducement of chondrogenesis of hMSCs. Biomaterials. 2012, 33: 7151-7163. 10.1016/j.biomaterials.2012.06.023.
Salinas CN, Cole BB, Kasko AM, Anseth KS: Chondrogenic differentiation potential of human mesenchymal stem cells photoencapsulated within poly(ethylene glycol)-arginine-glycine-aspartic acid-serine thiol-methacrylate mixed-mode networks. Tissue Eng. 2007, 13: 1025-1034. 10.1089/ten.2006.0126.
Ma K, Wu Y, Wang B, Yang S, Wei Y, Shao Z: Effect of a synthetic link N peptide nanofiber scaffold on the matrix deposition of aggrecan and type II collagen in rabbit notochordal cells. J Mater Sci Mater Med. 2013, 24: 405-415. 10.1007/s10856-012-4811-3.
Rosenzweig DH, Chicatun F, Nazhat SN, Quinn TM: Cartilaginous constructs using primary chondrocytes from continuous expansion culture seeded in dense collagen gels. Acta Biomater. 2013, 9: 9360-9369. 10.1016/j.actbio.2013.07.024.
Benders KE, van Weeren PR, Badylak SF, Saris DB, Dhert WJ, Malda J: Extracellular matrix scaffolds for cartilage and bone regeneration. Trends Biotechnol. 2013, 31: 169-176. 10.1016/j.tibtech.2012.12.004.
Yang Q, Peng J, Guo Q, Huang J, Zhang L, Yao J, Yang F, Wang S, Xu W, Wang A, Lu S: A cartilage ECM-derived 3-D porous acellular matrix scaffold for in vivo cartilage tissue engineering with PKH26-labeled chondrogenic bone marrow-derived mesenchymal stem cells. Biomaterials. 2008, 29: 2378-2387. 10.1016/j.biomaterials.2008.01.037.
Jin CZ, Park SR, Choi BH, Park K, Min BH: In vivo cartilage tissue engineering using a cell-derived extracellular matrix scaffold. Artif Organs. 2007, 31: 183-192. 10.1111/j.1525-1594.2007.00363.x.
Schwarz S, Elsaesser AF, Koerber L, Goldberg-Bockhorn E, Seitz AM, Bermueller C, Durselen L, Ignatius A, Breiter R, Rotter N: Processed xenogenic cartilage as innovative biomatrix for cartilage tissue engineering: effects on chondrocyte differentiation and function. J Tissue Eng Regen Med. 2012, doi:10.1002/term.1650
Gooch KJ, Kwon JH, Blunk T, Langer R, Freed LE, Vunjak-Novakovic G: Effects of mixing intensity on tissue-engineered cartilage. Biotechnol Bioeng. 2001, 72: 402-407. 10.1002/1097-0290(20000220)72:4<402::AID-BIT1002>3.0.CO;2-Q.
Chadjichristos C, Ghayor C, Herrouin JF, Ala-Kokko L, Suske G, Pujol JP, Galera P: Down-regulation of human type II collagen gene expression by transforming growth factor-beta 1 (TGF-beta 1) in articular chondrocytes involves SP3/SP1 ratio. J Biol Chem. 2002, 277: 43903-43917. 10.1074/jbc.M206111200.
Galera P, Redini F, Vivien D, Bonaventure J, Penfornis H, Loyau G, Pujol JP: Effect of transforming growth factor-beta 1 (TGF-beta 1) on matrix synthesis by monolayer cultures of rabbit articular chondrocytes during the dedifferentiation process. Exp Cell Res. 1992, 200: 379-392. 10.1016/0014-4827(92)90186-C.
Galera P, Vivien D, Pronost S, Bonaventure J, Redini F, Loyau G, Pujol JP: Transforming growth factor-beta 1 (TGF-beta 1) up-regulation of collagen type II in primary cultures of rabbit articular chondrocytes (RAC) involves increased mRNA levels without affecting mRNA stability and procollagen processing. J Cell Physiol. 1992, 153: 596-606. 10.1002/jcp.1041530322.
Hicks DL, Sage AB, Shelton E, Schumacher BL, Sah RL, Watson D: Effect of bone morphogenetic proteins 2 and 7 on septal chondrocytes in alginate. Otolaryngol Head Neck Surg. 2007, 136: 373-379. 10.1016/j.otohns.2006.10.040.
Abukawa H, Oriel BS, Leaf J, Vacanti JP, Kaban LB, Troulis MJ, Hartnick CJ: Growth factor directed chondrogenic differentiation of porcine bone marrow-derived progenitor cells. J Craniofac Surg. 2013, 24: 1026-1030. 10.1097/SCS.0b013e31827ff323.
Itoh S, Hattori T, Tomita N, Aoyama E, Yutani Y, Yamashiro T, Takigawa M: CCN family member 2/connective tissue growth factor (CCN2/CTGF) Has anti-aging effects that protect articular cartilage from Age-related degenerative changes. PLoS One. 2013, 8: e71156-10.1371/journal.pone.0071156.
Furumatsu T, Matsumoto E, Kanazawa T, Fujii M, Lu Z, Kajiki R, Ozaki T: Tensile strain increases expression of CCN2 and COL2A1 by activating TGF-beta-Smad2/3 pathway in chondrocytic cells. J Biomech. 2013, 46: 1508-1515. 10.1016/j.jbiomech.2013.03.028.
Tomita N, Hattori T, Itoh S, Aoyama E, Yao M, Yamashiro T, Takigawa M: Cartilage-specific over-expression of CCN family member 2/connective tissue growth factor (CCN2/CTGF) stimulates insulin-like growth factor expression and bone growth. PLoS One. 2013, 8: e59226-10.1371/journal.pone.0059226.
Kubota S, Takigawa M: The role of CCN2 in cartilage and bone development. J Cell Commun Signal. 2011, 5: 209-217. 10.1007/s12079-011-0123-5.
Wang J, Elewaut D, Hoffman I, Veys EM, Verbruggen G: Physiological levels of hydrocortisone maintain an optimal chondrocyte extracellular matrix metabolism. Ann Rheum Dis. 2004, 63: 61-66. 10.1136/ard.2002.005298.
Zhang L, Zhang X, Li KF, Li DX, Xiao YM, Fan YJ, Zhang XD: Icariin promotes extracellular matrix synthesis and gene expression of chondrocytes in vitro. Phytother Res. 2012, 26: 1385-1392. 10.1002/ptr.3733.
Li D, Yuan T, Zhang X, Xiao Y, Wang R, Fan Y: Icariin: a potential promoting compound for cartilage tissue engineering. Osteoarthritis Cartilage. 2012, 20: 1647-1656. 10.1016/j.joca.2012.08.009.
Lee HS, Park SY, Park Y, Bae SH, Suh HJ: Yeast hydrolysate protects cartilage via stimulation of type II collagen synthesis and suppression of MMP-13 production. Phytother Res. 2013, 27: 1414-1418. 10.1002/ptr.4857.
Tong J, Yao S: Novel scaffold containing transforming growth factor-beta 1 DNA for cartilage tissue engineering. J Bioact Compat Polym. 2007, 22: 232-244. 10.1177/0883911507076460.
Song J, Lee M, Kim D, Han J, Chun CH, Jin EJ: MicroRNA-181b regulates articular chondrocytes differentiation and cartilage integrity. Biochem Biophys Res Commun. 2013, 431: 210-214. 10.1016/j.bbrc.2012.12.133.
Yang B, Guo H, Zhang Y, Chen L, Ying D, Dong S: MicroRNA-145 regulates chondrogenic differentiation of mesenchymal stem cells by targeting Sox9. PLoS One. 2011, 6: e21679-10.1371/journal.pone.0021679.
Legendre F, Dudhia J, Pujol JP, Bogdanowicz P: JAK/STAT but not ERK1/ERK2 pathway mediates interleukin (IL)-6/soluble IL-6R down-regulation of Type II collagen, aggrecan core, and link protein transcription in articular chondrocytes. Association with a down-regulation of SOX9 expression. J Biol Chem. 2003, 278: 2903-2912. 10.1074/jbc.M110773200.
Ryu B, Himaya SW, Napitupulu RJ, Eom TK, Kim SK: Sulfated chitooligosaccharide II (SCOS II) suppress collagen degradation in TNF-induced chondrosarcoma cells via NF-kappaB pathway. Carbohydr Res. 2012, 350: 55-61.
Zhang F, Yao Y, Su K, Fang Y, Citra F, Wang DA: Co-transduction of lentiviral and adenoviral vectors for co-delivery of growth factor and shRNA genes in mesenchymal stem cells-based chondrogenic system. J Tissue Eng Regen Med. 2012, doi:10.1002/term.1656
Hansen U, Schunke M, Domm C, Ioannidis N, Hassenpflug J, Gehrke T, Kurz B: Combination of reduced oxygen tension and intermittent hydrostatic pressure: a useful tool in articular cartilage tissue engineering. J Biomech. 2001, 34: 941-949. 10.1016/S0021-9290(01)00050-1.
Scherer K, Schunke M, Sellckau R, Hassenpflug J, Kurz B: The influence of oxygen and hydrostatic pressure on articular chondrocytes and adherent bone marrow cells in vitro. Biorheology. 2004, 41: 323-333.
Waldman SD, Couto DC, Grynpas MD, Pilliar RM, Kandel RA: A single application of cyclic loading can accelerate matrix deposition and enhance the properties of tissue-engineered cartilage. Osteoarthritis Cartilage. 2006, 14: 323-330. 10.1016/j.joca.2005.10.007.
Hu JC, Athanasiou KA: The effects of intermittent hydrostatic pressure on self-assembled articular cartilage constructs. Tissue Eng. 2006, 12: 1337-1344. 10.1089/ten.2006.12.1337.
Kanazawa T, Furumatsu T, Hachioji M, Oohashi T, Ninomiya Y, Ozaki T: Mechanical stretch enhances COL2A1 expression on chromatin by inducing SOX9 nuclear translocalization in inner meniscus cells. J Orthop Res. 2012, 30: 468-474. 10.1002/jor.21528.
Freed LE, Marquis JC, Langer R, Vunjak-Novakovic G, Emmanual J: Composition of cell-polymer cartilage implants. Biotechnol Bioeng. 1994, 43: 605-614. 10.1002/bit.260430710.
Bueno EM, Bilgen B, Barabino GA: Wavy-walled bioreactor supports increased cell proliferation and matrix deposition in engineered cartilage constructs. Tissue Eng. 2005, 11: 1699-1709. 10.1089/ten.2005.11.1699.
Lee CR, Grodzinsky AJ, Spector M: Biosynthetic response of passaged chondrocytes in a type II collagen scaffold to mechanical compression. J Biomed Mater Res A. 2003, 64: 560-569.
Albrecht C, Tichy B, Nurnberger S, Zak L, Handl MJ, Marlovits S, Aldrian S: Influence of cryopreservation, cultivation time and patient’s age on gene expression in Hyalograft(R) C cartilage transplants. Int Orthop. 2013, 37: 2297-2303. 10.1007/s00264-013-2009-z.
Abazari A, Jomha NM, Elliott JA, McGann LE: Cryopreservation of articular cartilage. Cryobiology. 2013, 66: 201-209. 10.1016/j.cryobiol.2013.03.001.
Lyu SR, Kuo YC, Ku HF, Hsieh WH: Cryopreserved chondrocytes in porous biomaterials with surface elastin and poly-L-lysine for cartilage regeneration. Colloids Surf B Biointerfaces. 2013, 103: 304-309.
Xia Z, Duan X, Murray D, Triffitt JT, Price AJ: A method of isolating viable chondrocytes with proliferative capacity from cryopreserved human articular cartilage. Cell Tissue Bank. 2013, 14: 267-276. 10.1007/s10561-012-9328-y.
Our work was funded by Shenzhen Science and Technology Key Project (Project NO. 201001013) and Shenzhen Science and Technology International Cooperative Project (Project NO. 20130326210545).
The authors declare that they have no competing interests.
CJL conceived of the study, participated in the experiments and drafted the manuscript; DL participated in the design of the draft and revised it critically for important intellectual content; ZW participated in the experiments and interpreted the data obtained; XJ critically revised the draft for important intellectual content and obtained funding; WD supervised the study, revised the draft for important intellectual content, obtained funding and approval of the final version of the manuscript. All authors read and approved the final manuscript.
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Chen, J., Duan, L., Zhu, W. et al. Extracellular matrix production in vitro in cartilage tissue engineering. J Transl Med 12, 88 (2014). https://doi.org/10.1186/1479-5876-12-88
- Tissue engineering
- Extracellular matrix
- Collagen type II