- Poster presentation
- Open Access
BAFF polymorphisms and serum levels of BAFF in Tunisian systemic lupus erythematosus patients
© Laamiri et al; licensee BioMed Central Ltd. 2012
- Published: 28 November 2012
- Systemic Lupus Erythematosus
- Systemic Lupus Erythematosus Patient
- Regulatory Region
- Autoantibody Production
- Single Allele
To investigate any associations between regulatory genetic polymorphisms of BAFF gene, disease susceptibility and serum BAFF (s-BAFF) levels in Tunisian systemic lupus (SLE) patients.
The case-control study included 124 SLE patients and 152 healthy controls. Three single nucleotide polymorphisms (SNPs) (-2841 T>C, -2701 A>T and -871 C>T) in the 5’ regulatory region of the BAFF gene were explored by PCR-RFLP . s-BAFF levels were measured by ELISA (R&D Systems).
s-BAFF levels were elevated in SLE patients (1717,08 pg/ml) and in anti-dsDNA positive antibodies patients (1948,28 pg/ml) compared to both controls (665,82 pg/ml, p<10-3) and patients without anti-dsDNA antibodies (1281,51 pg/ml, p:0,007). In contrast, no correlation was found between global disease activity registered in SLEDAI and s-BAFF levels (p: 0.7). Furthermore, no association was found between BAFF genotypes and susceptibility to SLE. Single allele, genotype and haplotype association analyses showed no significant association with s-BAFF values, clinical features or SLEDAI score in SLE.
Polymorphisms in the regulatory region of the BAFF gene do contribute neither to increased s-BAFF levels nor to the susceptibility to SLE in Tunisian patients. Increased s-BAFF levels in anti-dsDNA positive antibodies SLE patients suggest the central role of this molecule in the inflammatory process involving in autoantibodies production.
- Becker-Merok A, Nikolaisen C, Nossent HC: B-lymphocyte activating factor in systemic lupus erythematosus and rheumatoid arthritis in relation to autoantibody levels, disease measures and time. Lupus. 2006, 15: 570-6. 10.1177/0961203306071871.View ArticlePubMedGoogle Scholar
- Zhang J, Roscheke V, Baker KP: A role of B lymphocyte stimulator in systemic lupus erythematosus. J Immunol. 2001, 166: 6-10.View ArticlePubMedGoogle Scholar
- Eilertsen G, Van Ghelue M, Strand H, Nossent J: Increased levels of BAFF in patients with systemic lupus erythematosus are associated with acute-phase reactants, independent of BAFF genetics: a case-control study. Rheumatology. 2011, 50: 2197-2205. 10.1093/rheumatology/ker282.View ArticlePubMedGoogle Scholar
- Nossent JC, Lester S, Zahra D: Polymorphism in the 5’regulatory region of the B-lymphocyte activating factor gene is associated with the RO/La autoantibody response and serum BAFF levels in primary Sjogren’s syndrome. Rheumatology. 2008, 47: 1311-6. 10.1093/rheumatology/ken246.View ArticlePubMedGoogle Scholar
This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.