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Journal of Translational Medicine

Open Access

FcRn mediated IgG transport by retinal pigment epithelium cells

  • Nicoline M Korthagen1,
  • Kiki van Bilsen1, 2,
  • Willem A Dik1,
  • Jeroen Bastiaans1, 3,
  • Marion Kolijn1,
  • Robert W Kuijpers4 and
  • Martin van Hagen1, 2
Journal of Translational Medicine201210(Suppl 3):P2

Published: 28 November 2012


Retinal Pigment EpithelialChloroquineRetinal Pigment Epithelial CellImmortalize Cell LineHuman Retinal Pigment Epithelial


The neonatal Fc receptor (FcRn) binds immunoglobulin G (IgG), protects it from degradation and can transport IgG from apical to basolateral side of epithelial cells [1]. Previously, we showed that human retinal pigment epithelial (RPE) cells express FcRn and that, in contrast to non-ocular cells, TNF-α downregulates FcRn expression in RPE cells [2]. We speculated that FcRn in RPE plays a pivotal role in ocular IgG metabolism. The aim of this study was to develop a method to investigate whether RPE cells transport IgG molecules.

Materials and methods

Monolayers of RPE cells, ARPE-19 (immortalized cell line) and human primary RPE cells (both fetal and adult), were established on Transwell culture inserts using fibronectin coated membranes. Trans-epithelial resistance (TER) measurements were used to evaluate monolayer formation. Plates with an average TER >35Ω*cm2 were used for subsequent experiments. Monoclonal IgG was added either apically or basolaterally and after incubation IgG was measured in the culture supernatant using an ELISA that was developed inhouse. To distinguish leakage of IgG through the cell layer from active transport by the cells we used NH4Cl or Chloroquine to block cytoplasmic vesicles.


The highest TERs were observed with ARPE-19 cells, while TERs often remained low with the primary cell cultures. The IgG level that crossed the membrane correlated positively incubation time, but this association was not linear. The observed effects of NH4Cl and Chloroquine were limited.


Further experiments are needed to determine whether there is active transport by the RPEs. Paracellular diffusion will be investigated using dextran and specific blocking antibodies for the FcRn. In addition, experiments will be performed with RPE cells where FcRn is overexpressed or knocked-out.



Fetal human RPE cells were kindly provided by Sheldon Miller (National Eye Institute, Bethesda, MD, USA).

This research project was funded by grants from UitZicht and the International Retinal Research Foundation.

Authors’ Affiliations

Dept. of Immunology, Erasmus University Medical Center, Rotterdam, the Netherlands
Dept. of Internal Medicine, Erasmus University Medical Center, Rotterdam, the Netherlands
Rotterdam Eye Hospital, Rotterdam, the Netherlands
Dept. of Ophthalmology, Erasmus University Medical Center, Rotterdam, the Netherlands


  1. Roopenian DC, Akilesh S: FcRn: the neonatal Fc receptor comes of age. Nat Rev Immunol. 2007, 7 (9): 715-25. 10.1038/nri2155.View ArticlePubMedGoogle Scholar
  2. van Bilsen K, van Hagen PM, Bastiaans J: The neonatal Fc receptor is expressed by human retinal pigment epithelial cells and is downregulated by tumour necrosis factor-alpha. Br J Ophthalmol. 2011, 95 (6): 864-868. 10.1136/bjo.2010.187930.View ArticlePubMedGoogle Scholar


© Korthagen et al; licensee BioMed Central Ltd. 2012

This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.