Ipilimumab augments antitumor activity of bispecific antibody-armed T cells
© Yano et al.; licensee BioMed Central Ltd. 2014
Received: 18 October 2013
Accepted: 5 June 2014
Published: 9 July 2014
Ipilimumab is an antagonistic monoclonal antibody against cytotoxic T-lymphocyte antigen-4 (CTLA-4) that enhances antitumor immunity by inhibiting immunosuppressive activity of regulatory T cells (Treg). In this study, we investigated whether inhibiting Treg activity with ipilimumab during ex vivo T cell expansion could augment anti-CD3-driven T cell proliferation and enhance bispecific antibody (BiAb)-redirected antitumor cytotoxicity of activated T cells (ATC).
PBMC from healthy individuals were stimulated with anti-CD3 monoclonal antibody with or without ipilimumab and expanded for 10-14 days. ATC were harvested and armed with anti-CD3 x anti-EGFR BiAb (EGFRBi) or anti-CD3 x anti-CD20 BiAb (CD20Bi) to test for redirected cytotoxicity against COLO356/FG pancreatic cancer cell line or Burkitt’s lymphoma cell line (Daudi).
In PBMC from healthy individuals, the addition of ipilimumab at the initiation of culture significantly enhanced T cell proliferation (p = 0.0029). ATC grown in the presence of ipilimumab showed significantly increased mean tumor-specific cytotoxicity at effector:target (E:T) ratio of 25:1 directed at COLO356/FG and Daudi by 37.71% (p < 0.0004) and 27.5% (p < 0.0004), respectively, and increased the secretion of chemokines (CCL2, CCL3, CCL4,CCL5, CXCL9, and granulocyte-macrophage colony stimulating factor(GM-CSF)) and cytokines (IFN-γ, IL-2R, IL-12, and IL-13), while reducing IL-10 secretion.
Expansion of ATC in the presence of ipilimumab significantly improves not only the T cell proliferation but it also enhances cytokine secretion and the specific cytotoxicity of T cells armed with bispecific antibodies.
KeywordsIpilimumab Cytotoxic T-lymphocyte antigen-4 (CTLA-4) Bispecific antibody Pancreatic cancer Colorectal cancer Burkitt’s lymphoma Activated T cells Immunotherapy
Efficacy of cancer immunotherapy using targeted T cells has been limited by tumor-induced immunosuppression or regulatory T cells (Tregs) that interfere with T cell effector functions resulting in failure to induce robust cellular and humoral antitumor responses. Since recent clinical trial data show that cytotoxic T-lymphocyte antigen-4 (CTLA-4) positive Tregs are associated with reduced T cell antitumor activities, we hypothesized that treatment with anti-CTLA-4 monoclonal antibody (mAb) during T cell expansion would augment the proliferative and functional activities of activated T cells.
CTLA-4 is constitutively expressed on CD4+CD25highforkhead box P3+ (FOXP3+) Treg cells, transiently on newly activated T cells and minimally or negative on naïve T cells[3, 4]. CTLA-4 is homologous to the co-stimulatory receptor CD28 but competes for CD80 and CD86 binding with much higher avidity[5–7] leading to suppression of T cell activation[8, 9]. CLTA-4 blockade studies have demonstrated greater tumor regression in antibody-treated mice[10–12]. Furthermore, Treg-depletion prior to blocking the CTLA-4 signaling pathway results in enhanced T cell proliferation and tumor-specific cytotoxicity in vivo[2, 13] and in vitro.
Ipilimumab (Yervoy®, Bristol-Myers Squibb) is a human mAb antibody directed at CTLA-4 that inhibits the binding of CLTA-4 to CD80 and CD86 during T cell activation, therefore increasing the CD28 binding opportunity to the co-stimulatory factors for appropriate T cell activation. In the FDA registration trial, ipilimumab significantly improved the median overall survival from 6.4 months in the group that received gp100 alone to 10.0 or 10.1 months in the group that received ipilimumab with or without gp100 (cancer vaccine comprises HLA-A*0201–restricted peptides derived from the melanosomal protein, glycoprotein 100 [gp100]), respectively, in patients with unresectable stage III and IV melanoma. However, 88.9% of the patients experienced dermatologic, gastrointestinal, or other immune-related adverse events (irAEs) from ipilimumab of which 10-15% were grade 3 or 4 that resulted in 2.1% drug-related deaths. Therefore, new approaches that provide the antitumor effects of ipilimumab without the side effects are needed. One approach to avoid irAEs would be by adding ipilimumab at the initiation of T cell expansion cultures to enhance the proliferation and cytotoxicity mediated by BiAb-armed ATC. This study shows that ipilimumab enhances T cell proliferation, tumor-specific cytotoxicity mediated by BiAb, and cytokine secretion without Treg-depletion prior to ATC expansion. These results could be used to optimize antitumor activity in immunotherapeutic approaches for patients with oncological or hematological malignancies.
Materials and methods
The human Burkitt’s lymphoma cell line (Daudi) and human pancreatic cancer cell line (COLO356/FG) were maintained in RPMI-1640 or DMEM culture media (Lonza Inc., Allendale, NJ), respectively, supplemented with 10% fetal bovine serum (FBS) (Valley Biomedical Inc., Winchester, VA), 2 mM L-glutamine (Lonza Inc.), 50 IU/mL penicillin and 50 μg/mL streptomycin (Lonza Inc.).
Generation of ATC
PBMC for ATC expansion were obtained from healthy volunteers (KCI protocol 2007-012) and leukapheresis samples from GI patients which were cryopreserved prior to immunotherapy (KCI protocol 2011-025). Both protocols were approved by the Wayne State University Human Investigation Committee. T cells in the PBMC were activated by 20 ng/mL of anti-CD3 monoclonal antibody (OKT3, Ortho Biotech, Horsham, PA) and expanded with 100 IU/mL of Interleukin-2 (IL-2) in RPMI-1640 supplemented with 10% FBS, 2 mM L-glutamine, 50 units/mL penicillin and 50 μg/mL streptomycin (complete RPMI-1640) for 14 days at 37°C with 5% CO2. At the initiation of culture, ipilimumab (YERVOY® by Bristol-Myer Squibb, Princeton, NJ) was added only once to the cultures at various concentrations (0, 0.5, 5.0, and 50 μg/mL). Cultures were fed every 2 to 3 days and maintained at a concentration of 1x106 cells/mL. IL-2 was added at every feeding at 100 IU/mL.
Production of bispecific antibodies and arming of ATC
Bispecific antibodies (BiAbs) were produced by chemical heteroconjugation of OKT3 and Rituxan (a humanized anti-CD20 IgG1, Genentech Inc., South San Francisco, CA) or Erbitux (a humanized anti-epidermal growth factor receptor (EGFR) IgG1, ImClone LLC., Branchburg, NJ) as described[15, 16]. ATC were armed with anti-OKT3 x anti-CD20 BiAb (CD20Bi) or anti-OKT3 x anti-EGFR BiAb (EGFRBi) using a previously optimized concentration (50 ng/106 ATC).
Cytotoxicity/51Cr release assay
To target adherent cells, COLO356/FG cells were plated in 96-well flat-bottom microtiter plates at 4x104 cells/well and allowed to adhere overnight. The cells were labeled with 51Cr at 20 μCi/mL in the labeling media (50% FBS in complete RPMI-1640) for 5 hours at 37°C, and washed with complete RPMI-1640 to remove unincorporated isotope. For non-adherent cell targeting, Daudi cells were labeled with 51Cr at 100 μCi/106 cells in a 15 mL conical tube for 4 hours at 37°C, washed with complete RPMI-1640, and plated in 96-well round-bottom microtiter plates at 1x104 cells/well. Effectors (unarmed ATC and aATC) were then added to achieve effector:target (E:T) ratios of 25:1 and 12.5:1. Co-cultures were incubated for 4 hours (Daudi) or 18 hours (COLO356/FG) and the supernatant was collected for liquid scintillation counting to quantitate the amount of released 51Cr. Percent cytotoxicity was calculated as follows: (experimental cpm – spontaneous cpm)/(maximum cpm – spontaneous cpm) × 100. Means and standard errors were calculated from four to six replicates per sample.
T cell sub-population profiling
Changes in T cell sub-populations were quantitated by flow cytometric analysis as described[18, 19]. CD8-PE-Cy5, CD16-PE, CD25-APC, CD45RA-FITC, CD45RO-PE, CD56-APC, CD127-PE, and CD152-PE-Cy5 were purchased from BD Biosciences (San Jose, CA). CD3-FITC and CD4-APC were purchased from Miltenyi Biotec (Auburn, CA).
Quantitation of released cytokines in co-cultures
ATC expanded with or without ipilimumab were armed with EGFRBi and co-cultured with COLO356/FG at 10:1 E:T ratio. The amount of cytokines released by unarmed ATC or aATC in the co-culture supernatant was quantitated using 25-plex human cytokine Luminex Assay (Invitrogen, Carlsbad, CA) in the Bio-Plex System (Bio-Rad Lab., Hercules, CA) as described[19, 20]. Analysis panel includes IL-1β, IL-1 receptor antagonist (IL-1Ra), IL-2, IL-2R, IL-4, IL-5, IL-6, IL-7, IL-8, IL-13, IL-17, tumor necrosis factor (TNF)-α, interferon (IFN)-α, IFN-γ, granulocyte macrophage colony-stimulating factor (GM-CSF), macrophage inhibitory protein (MIP)-1α (CCL3), MIP-1β (CCL4), interferon inducible protein (IP)-10, monokine induced by IFN-γ (MIG or CXCL9), eotaxin, regulated on activation normal T cell expressed and secreted (RANTES or CCL5), and monocyte chemotactic protein (MCP)-1 (CCL2). Bio-Plex Manager Software was used to calculate the cytokine concentrations using a recombinant cytokines-derived standard curve.
Quantitative data are presented as the means of at least three or more independent experiments with standard errors. Paired, two-tailed t-test and matched sample one-way ANOVA were used to determine whether the data were statistically significant.
Ipilimumab affects T cell effector functions in dose dependent manner
Enhanced cytotoxicity is sustained at lower E:T
Increased cytotoxicity by BiAb-armed T cells from patients with gastrointestinal (GI) cancers in the presence of ipilimumab
Ipilimumab enhances proliferation of activated T cells
Both in vivo and in vitro studies have shown that blocking CTLA-4 signaling enhances T cell proliferation, and in some cases, synergistic enhancement was observed when Treg cells were depleted prior to T cell stimulation[2, 13]. We examined an effect of ipilimumab on the proliferation of ATC from PBMC without physical Treg-depletion. T cell proliferation was monitored by measuring the cell concentrations each time the cultures were fed. As shown in the lower panel of Figure 3, ATC proliferation increased by 18.61% (p = 0.0029) in the presence of ipilimumab.
Ipilimumab decreases CD4/CD8 ratio and Treg population in expanded ATC
Secretion of immune activating chemokines and cytokines were elevated by ipilimumab
Previously we have shown that T cells expanded from the leukapheresis product or PBMC from the immunosuppressive environment of cancer patients can be stimulated to improve their functions in vitro as well as in vivo[30, 31]. ATC armed with anti-CD3 x anti-tumor associated antigen BiAbs exhibit high levels of specific cytotoxicity against tumor cells expressing Her2/neu[17, 18, 32], CD20, GD2, and EGFR[16, 19] via redirected non-MHC-restricted perforin/granzyme-dependent killing. Furthermore, arming with BiAbs creates artificial cytotoxic T lymphocytes wherein TCR engagement via BiAb-bridge between the armed ATC and the antigen on the tumor cells induces release of significantly higher levels of IFN-γ, IL-2R, IL-12, CCL3, CCL4 and CXCL9, compared to unarmed ATC used as controls. In this study, we demonstrated that the addition of ipilimumab at the initiation of culture significantly improves T cell proliferation, BiAb-mediated tumor-specific cytotoxicity, and synthesis of inflammatory chemokines and cytokines. Phenotypic analysis of T cell subpopulations showed a decreased Tregs population with a concomitant decrease in IL-10 secretion as well as a reduced CD4/CD8 ratio in the ATC products expanded in the presence of ipilimumab. The reduced CD4/CD8 ratio may account for the increased specific cytotoxicity. These data demonstrate that ipilimumab can improve ATC proliferation and effector functions while it diminishes immune suppressive Treg activity.
Enhanced T cell proliferation in the presence of ipilimumab may be due to the inhibition of the interaction between pre-existing Treg cells and monocytes/dendritic cells in PBMC that were indirectly activated upon anti-CD3 stimulation of T cells. In addition, it is likely that ipilimumab blocked the CTLA-4-mediated inhibitory signaling in activated effector T cells as CTLA-4 expression is known to peak around 2 days after anti-CD3 stimulation[3, 4]. However, it is not clear whether decreased CD4 percent population was due to a direct effect of ipilimumab on CD4 T cell proliferation or due to an enhanced CD8 T cell expansion induced by ipilimumab.
As shown in the Figure 4d, the NKT cell population in some healthy donors persisted during the ATC culture period in both control and ipilimumab ATC preparations with no significant difference. NK cell activation is a complex set of signal transduction pathways including direct cell-cell signaling from APCs and humoral signaling via cytokines and chemokines. Since the ATC culture was fed with IL-2, which is a NK cell stimulatory factor[34, 35], and antigen-bound IgG is also known to activate NK and NKT cells via Fcγ receptor bindings, NKT cells may be activated by IL-2 and Fc binding of ipilimumab bound to CTLA-4 on the surface of ATC which in turn may have exhibited the elevated secretion of INF-γ and CTL activity. Additional studies with purified NKT cells will be needed to address the effects of ipilimumab on NK and NKT cells.
The cytokine quantitation studies revealed that ipilimumab enhances the secretion of various cytokines and chemokines in a dose-dependent manner. Dose dependent (ipilimumab) increase in IFN-γ and IL-12 suggest generation of Th1 cytokines produced during armed ATC mediated killing of target cells. These cytokines and chemokines may activate endogenous immune cells in vivo[30, 31]. However, the role of soluble IL-2R (sIL-2R) in culture supernatant is not clear. Both Tregs and activated T cells can release IL-2R, and measurable levels of sIL-2R in the blood have been shown to indicate sustained immune activation. On the other hand, sIL-2R also competes for IL-2 binding on activated T cells, thereby inhibiting T cell proliferation[36, 37]. Increased release of sIL-2R has also been shown to induce differentiation of CD4 T cells into FOXP3+ Treg. Since proliferation and activation were both enhanced in the presence of ipilimumab, increased levels of sIL-2R in this study are likely due to the activation of T cells rather than its immune suppressive activity.
Intriguingly, decreased levels of IL-10 suggest that ipilimumab attenuates the release of IL-10 in dose-dependent manner. IL-10 has been shown to contribute in tumor derived immune suppression by suppressing CD4 T cells and inducing myeloid derived suppressor cells.
In summary, we demonstrated that ipilimumab can improve ATC proliferation, enhance the BiAb-mediated tumor-specific cytotoxicity, and increase cytokine synthesis, while it attenuates Treg activity as shown by decreased level of IL-10 secretion and reduced Treg population. This mechanism may be clinically relevant since ipilimumab treatment of PBMC from GI patients was able to enhance BiAb-mediated cytotoxicity of ATC directed at pancreatic cancer cell line COLO356/FG. The approach of using ipilimumab to optimize effector functions of ex vivo expanded ATC may improve the efficacy of BiAb-mediated antigen targeted adoptive T cell immunotherapy without increasing ipilimumab related toxicities.
Antigen presenting cells
Activated T cells
Cytotoxic T-lymphocyte antigen-4
- GI cancer:
Chemokine (C-C motif) ligand
Chemokine (C-X-C motif) ligand
Dulbecco’s modified Eagle’s medium
Epidermal growth factor receptor
Fetal bovine serum
The Food and Drug Administration
Granulocyte-macrophage colony-stimulating factor
Monocyte chemotactic protein
Macrophage inhibitory protein
Interferon inducible protein
Monokine induced by IFN-γ
Regulated on activation, normal T cell expressed and secreted
Natural killer cells
Natural killer T cells
Helper T cells
Peripheral blood mononuclear cells
Roswell Park Memorial Institute.
This study was supported in part by R01 CA 092344 (LGL) and R01 CA 140314 (LGL) from National Cancer Institute, the Ruth F. Rattner and Ann F. & Norman D. Katz Charitable Foundation, Ray and Lynn Wood Neag Foundation, Helen L. Key charitable Trust, and the Young Family Foundation.
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