Fig. 6

FCNB expression is upregulated and colocalized with CD68 in murine colitis model. A Schematic of the animal experiment design. Four-week-old C57BL/6 mice were treated with 3% DSS in drinking water for 7 days to induce colitis, while the control group received water. On the 8th day, mice were sacrificed for further analysis. B Mice with DSS-induced colitis showed significantly decreased body weight compared with the control mice. C Colon lengths were measured 8 days after DSS treatment. D Gross pictures of spleens and spleen index (%). Spleen index (%) is the ratio of spleen weight to body weight. E Representative H&E-stained colon tissue sections of control and colitis groups. Scale bars, 50 μM. F The mRNA expression levels of Fcnb (the murine homologue gene of human FCN1) in peripheral whole blood from control and colitis groups were measured by qRT-PCR. The protein expression levels of FCNB and IL-1β in colon G and spleen H were measured by Western blotting. β-actin was used as a loading control. I Immunohistochemistry staining of FCNB in colon of mice. Scale bars, 20 μM. J Immunofluorescence staining showing that FCNB⁺ cells (red) colocalize with CD68⁺cells (green) in the colon from mice with DSS-induced colitis. Yellow indicates double positive cells. Scale bars, 20 μM. Data represent mean ± SD. Unpaired t test was used to calculate P values. *P < 0.05, ***P < 0.001, ****P < 0.0001