Fig. 7

Ang-(1–7) maintained mitochondrial dynamic equilibrium in H9c2 cardiomyocytes and alleviated LPS-induced apoptosis. A Western blot images of Drp1, Mfn2, caspase-9, cleaved caspase-3, Bcl-2, and Bax in H9c2 cells. B–G Quantification of the concentrations of cleaved caspase-3, Drp1, Mfn2, Bcl-2, caspase-9, and Bax proteins in H9c2 cells (n = 4). H, I Flow cytometry plots and quantitative analysis depicting the proportion of H9c2 apoptotic cells (n = 4). J Before LPS stimulation of H9c2 cells for 12 h, cells in each group were pretreated using differing concentrations of A-779 (10^–5,10^–6,10^–7 mol/L) for 2 h, using Ang-(1–7) (10^–6 mol/L) for 1 h and then with CCK8 assay for cell viability (n = 6). ^*p < 0.05 than the control group, ^#p < 0.05 than LPS group, ^&p < 0.05 in comparison to LPS + Ang-(1–7) group. K, L) The IL-6 and TNF-α protein concentrations in H9c2 cells (n = 6). M–P IL-6, Bcl-2, TNF-α, and the Bax mRNA levels in the H9c2 cells (n = 4). K–P The A-779 intervention was pretreatment with 10^–5 mol/L for 2 h, the Ang-(1–7) intervention included 10^–6 mol/L pretreatment for 1 h, and LPS activation of 1 µg/mL for 12 h. ^*p < 0.05, ^**p < 0.01, ^***p < 0.001