Fig. 4

SNORD104 upregulated PARP1 expression in vitro and in vivo. A Immunoprecipitation of SNORD104 using anti-FBL antibodies. B Immunoblot showing FBL protein levels in SNORD104-overexpressing HEC1B cells. C The 2ʹ-O-methylation level up-regulated RNA in the Nm-seq data set was intersected with the RNA data set of SNORD104-Blast, and the KEGG pathway enrichment analysis showed that the apoptotic pathway ranked first. D Anti-FBL antibody was used to perform RIP assay in HEC1B cells, and the mRNA enrichment of PARP1, ITPR3, TUBA1C, PARP4, LMNB1 was detected by qRT-PCR. E Evaluation of PARP1, ITPR3, TUBA1C, PARP4, LMNB1 protein levels after overexpression of SNORD104, and only PARP1 RNA and protein level were changed. F PARP1 mRNA and protein levels in SNORD104-knockdown Ishikawa cells. G SNORD104 and PARP1 mRNA expression in EC tissues (n = 71) and the correlation between the expression levels (R = 0.3222, P < 0.0001). H PARP1 protein levels in the nuclear fraction of SNORD104-overexpressing cells. I, J PARP1 protein levels in the SNORD104-overexpressing subcutaneous xenografts in nude mice. *P < 0.05, ns means P > 0.05