Fig. 1

Radiation-induced gene expression profiles in mouse heart tissue. Whole genome microarray analysis was performed on all samples. A linear model was fit to each probe to evaluate differential expression of irradiated samples compared to controls. Criteria of |log₂Fold Change (FC)| > 1 and Benajmini-Hochberg adjusted (B-H) p-value < 0.05 relative to controls were used to determine significance and differential expression. A Heatmap displays expression patterns, represented by z-score, of all differentially expressed mRNAs across all doses and controls. B Venn diagram shows dose distribution and overlap of differentially expressed mRNAs across all doses. C The number of down-regulated versus up-regulated mRNAs at each dose are shown in the table. D Examples of significant linearly up- and down-regulated mRNAs are shown to display the dose response to radiation in heart tissue samples