- Letter to the Editor
- Open Access
Non-invasive monitoring of pH and oxygen using miniaturized electrochemical sensors
Journal of Translational Medicine volume 19, Article number: 252 (2021)
We read with great interest a recent published article by Pla et al.  in Journal of Translational Medicine. In this study, the authors investigated some variables including partial pressure of oxygen, pH, lactate, bicarbonate, and potassium in different time-points of hypoxia induction (i.e., basal, hypoxia-acidosis, and recovery periods). Therefore, they compared these variables between different time-points of measurement in one sample of 27 New Zealand White male rabbits. Since their comparisons were within-group, they were dependent to each others. However, they stated in abstract and statistical analysis section of the methods that one-way analysis of variance (ANOVA) was used to compare variables between different time-points of measurement. One-way ANOVA is used to compare numerical variables with normal distribution between more than two independent groups [2,3,4,5,6]. Because the comparisons in this study was performed between different time-points of measurements in one group of animals, the authors must assess normal distribution of numerical variables and then use repeated measures ANOVA or Friedman test [7,8,9,10]. Furthermore, they used student’s t-test for comparison of numerical variables between each two time-points of measurement, while they must use paired t-test or Wilcoxon for comparison of numerical variables between two time-points of measurement.
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Pla L, Berdún S, Mir M, Rivas L, Miserere S, Dulay S, Samitier J, Eixarch E, Illa M, Gratacós E. Non-invasive monitoring of pH and oxygen using miniaturized electrochemical sensors in an animal model of acute hypoxia. J Transl Med. 2021;19:1–11.
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Farrokhi, M., Manavi, S.P. & Taheri, F. Non-invasive monitoring of pH and oxygen using miniaturized electrochemical sensors. J Transl Med 19, 252 (2021). https://doi.org/10.1186/s12967-021-02923-1