Fig. 5

mTOR regulates CRP-induced phosphorylation of PKCs. Platelets from WT (W) or mTOR^−/− (KO, K) mice were stimulated with CRP at the indicated low concentration for 5.5 min. Lysates of platelets were immunoblotted with antibodies against a, b phospho-PKCα Thr497 and PKCα, c, d phospho-PKCβ Thr641 and PKCβ, c, e phospho-PKCβ Ser660 and PKC β, f, g phospho-PKCθ Thr538 and PKCθ, h, i phospho-PKCδ Thr505 and PKCδ, and j, k phospho-PKCε Ser729 and PKCε; these images (separated by horizontal white space) were cropped from the different/same gels and full-length/original blots were shown in Additional file 3: Part III. Phosphoprotein levels were normalized to total protein levels and were standardized to 1 in unstimulated WT samples. Values represent means ± SEM from at least three independent experiments (*P < 0.05, **P < 0.01; paired Student’s t test)