Fig. 4

mTOR deficiency impairs low-dose CRP induced phosphorylation of S6K1, S6, and Akt Ser473. Platelets from WT (W) or mTOR^−/− (KO, K) mice were stimulated with CRP at the indicated low concentration under aggregating conditions for 5.5 min. Lysates of platelets were immunoblotted with antibodies to a, b phospho-S6K1 Thr389 and S6K1, c, d phospho-S6 Ser235/236 and S6, or e, f phospho-Akt Ser473 and Akt; these images (separated by horizontal white space) were cropped from the different/same gels and full-length/original blots were shown in Additional file 3: Part III. Phosphoprotein levels were normalized to S6K1 levels for panel b, to S6 levels for panel c, and to Akt levels for panels e. Relative values were standardized to 1 in unstimulated WT samples and represent means ± SEM from at least three independent experiments (*P < 0.05, **P < 0.01; paired Student’s t test)