Immune cell phenotyping in low blood volumes for assessment of cardiovascular disease risk, development, and progression: a pilot study

Table 2 Percentage of all cell types in 500 µl of EDTA-heparinized whole blood stratified by ethnicity

	Caucasian (n = 15)	African-American (n = 14)	p-value
Median age	60 ± 12.7	56.5 ± 17.5	0.48
Granulocytes	72.9%	49.0%	0.008**
Neutrophils	63.95%	48.61%	0.02*
Eosinophils	2.64%	0.64%	0.03*
Basophils	0.07%	0.02%	0.06
Lymphocytes	16.7%	33.75%	0.008**
T cells	12.25%	25.15%	0.009**
B cells	1.02%	2.08%	0.05*
NKT cells	0.34%	0.87%	0.05*
NK cells	2.82%	4.09%	0.33
Proliferative NK cells (CD56^hiCD16^dim)	3.21%	3.50%	0.44
Cytotoxic NK cells (CD56^dim/CD16^hi)	91.3%	87.5%	0.001**
Monocytes	7.96%	7.59%	0.64
Classical monocytes (CD14⁺CD16⁻)	94.58%	89.88%	0.01*
Intermediate monocytes (CD14⁺CD16⁺)	2.61%	5.74%	0.01*
Non-classical monocytes (CD16⁺CD14⁻)	2.74%	5.04%	0.17
Platelet aggregates
Neutrophils	2.54%	2.60%	0.50
Eosinophils	1.6%	0.96%	0.08
Basophils	3.25%	0.00%	0.10
T cells	1.97%	1.62%	0.29
B cells	2.96%	2.62%	0.45
NK cells	2.44%	1.67%	0.86
NKT cells	2.37%	1.2%	0.23
All monocytes	4.2%	2.39%	0.98
Classical monocytes (CD14⁺CD16⁻)	3.29%	2.01%	0.19
Intermediate monocytes (CD14⁺CD16⁻)	5.28%	3.20%	0.35
Non-classical monocytes (CD14⁻CD16⁺)	3.79%	6.68%	0.04*

Data are shown as median percentage of all single, live, CD45-positive cells. Results of subsets are shown as percentage of the parent gate (NK cells or monocytes). Statistical significance was determined after Mann-Whitney test. Significance is indicated by asterisk. Correspondent p-values are listed
Italic values indicate significant differences between the two groups
*p ≤ 0.05; **p < 0.01

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