- Open Access
Human umbilical cord blood-derived mononuclear cell transplantation: case series of 30 subjects with Hereditary Ataxia
© Yang et al; licensee BioMed Central Ltd. 2011
- Received: 30 January 2011
- Accepted: 16 May 2011
- Published: 16 May 2011
The differential diagnosis for hereditary ataxia encompasses a variety of diseases characterized by both autosomal dominant and recessive inheritance. There are no curative treatments available for these neurodegenerative conditions. This open label treatment study used human umbilical cord blood-derived mononuclear cells (CBMC) combined with rehabilitation training as potential disease modulators.
30 patients suffering from hereditary ataxia were treated with CBMCs administered systemically by intravenous infusion and intrathecally by either cervical or lumbar puncture. Primary endpoint measures were the Berg Balance Scale (BBS), serum markers of immunoglobulin and T-cell subsets, measured at baseline and pre-determined times post-treatment.
A reduction of pathological symptoms and signs was shown following treatment. The BBS scores, IgG, IgA, total T cells and CD3+CD4 T cells all improved significantly compared to pre-treatment values (P < 0.01~0.001). There were no adverse events.
The combination of CBMC infusion and rehabilitation training may be a safe and effective treatment for ataxia, which dramatically improves patients' functional symptoms. These data support expanded double blind, placebo-controlled studies for these treatment modalities.
- Amyotrophic Lateral Sclerosis
- Spinocerebellar Ataxia
- Human Umbilical Cord
- Berg Balance Scale
- Rehabilitation Training
Hereditary ataxias are a heterogeneous group of neurodegenerative disorders, characterized by degenerative atrophy of the cerebellum, brain stem and/or spinal cord. The primary sequelae are clinical manifestations of dysarthria, dyscoordination of limbs, instability of gait, and eventual loss of posture [1–3]. Spinocerebellar ataxia (SCA) and Friedreich's ataxia (FRDA) are the most common forms of hereditary ataxia. Genetic anticipation usually occurs in familial patients, with symptoms and signs getting more severe with each successive generation [2, 3]. The disease is characterized by progressively disabling clinical manifestations. Patients show symptoms of gait instability or dysarthria and may begin to fall without warning. Gradually they present progressive limitations in their activities, lose the ability to walk, become bedridden and fully dependent, and most commonly succumb to pulmonary infection as the cause of death [2, 4].
To date, no effective routine therapy is currently available for hereditary ataxia [5–7]. Stem cell therapies were recently studied as an option to treat neurodegenerative disorders as it may provide neuroprotection and possibly promote regeneration [8–13]. In addition, studies on animal models [14, 15] and humans [16, 17] reported the therapeutic safety and efficacy of stem cell transplantation in cerebellar ataxia. Human umbilical cord blood (hUCB) proved to be a rich source of pluripotent stem cells for clinical application in neurodegenerative diseases [18, 19]. The mononuclear cells derived from hUCB are mainly comprised of a heterogenous population of hematopoietic and mesenchymal stem cells, endothelial progenitor cells and immature immunological cells [16, 20]. In this study, CBMC transplantation was examined as a potential therapy for hereditary ataxia. Thirty sequential patients with hereditary ataxias were treated with non-matched, allogeneic CBMCs. Treatment included both intravenous and intrathecal infusion of CBMCs, combined with proprioceptive neuromuscular facilitation. Our results indicate this combined treatment improved ataxia patients' functionality and quality of life.
Thirty patients with hereditary ataxia were recruited between January 2006 - May 2007 from the Nanshan Affiliated Hospital of Guangdong Medical College. Twenty five subjects had confirmed SCA (Type 1: 1 case, Type 2: 8 cases, Type 3: 5 cases, Type 6: 4 cases, unidentified genotype: 7 cases) and 5 cases of FRDA. The mean age was 43.14 ± 12.77 (range 19 to 71 years). The male-female gender ratio was 18:12. On average, patients had ataxias for 10.74 ± 5.89 years. The longest disease duration at the time of treatment was 26 years. Patients treated came from Australia, Britain, Canada, China, Chile, Italy, South Africa and U.S.A. There were no significant demographic or baseline co-morbidity differences in the 30 subject cohort.
The brain and cord MRI (Symphony 1.5T, Siemens, Germany) confirmed atrophy in the cerebellar hemispheres combined with atrophies at different levels in the brainstem and the cervical and thoracic segments of the spinal cord, but there were no signs of organic changes to the brain parenchyma. As per protocol, the pre- and post-treatment study tested for complete blood counts, routine urine tests, liver function, renal function, electrolytes, sero-enzymology, blood glucose, blood lipids, cellular and humoral immunity, routine cerebro-spinal fluid (CSF) and biochemical markers (biochemistry analyzer, Beckman, US and Epics-XL flow cytometer, Beckman, US).
All subjects were hospitalized while receiving CBMC transplantations. The CBMCs were provided by Shenzhen Beike Biotechnology Co., Ltd. after hUCB collection and mononuclear cell extraction, cultivation and harvest . Approximately 1-3 × 107 CBMCs were transfused per injection. Patients received both intrathecal and intravenous injections. The protocol, patient consent, and safety measures were approved by the local institutional review board of the Nanshan Affiliated Hospital of Guangdong Medical College under the auspices of the National Ministry of Health. Patients were explained the experimental nature of the procedure and informed consent was obtained from all patients before initiation of treatment. CBMCs were administered by intravenous infusion combined with intrathecal injection by either cervical or lumbar puncture. Each patient received cell transplantation four to six times - depending on the patient's condition, within an interval of five to seven days. Two ml of CSF was removed and replaced by 2 ml of cell suspension during the intrathecal injection. In terms of intravenous infusion, 30 ml of cell suspension was given through an intravenous catheter over 15-20 minutes. During stem cell treatment, rehabilitation cycles of balance training (proprioceptive neuromuscular facilitation) were given twice daily for four to six weeks, each cycle lasting 30 minutes. The major techniques employed in this training were: (1) visual compensation: the aim was to improve the proprioceptor sensitivity with the help of visual compensation; (2) using balance boards: the states tested were from static to moving; the support tope was from stable to unstable; eyes were from open to closed. A phased and sequenced manner was chosen based on the result of balance evaluation. The basis evaluation of treatment efficacy was executed with the Berg Balance Scale (BBS), which consists of 14 items assessing the ability to stand up and to maintain standing position despite internally produced perturbations . Each item is scored from 0 (unable) to 4 (safely done) with a maximum total score of 56 .
Criterion of therapeutic effect
There were no published criteria to measure therapeutic efficacy in the treatment of ataxia. We applied an accepted statistical methodology of 50% or greater improvement from baseline in BBS score. In order to quantitate the response further, >50% was deemed to be markedly effective; 5%-49% was classified as effective, while <5% improvement was deemed to be ineffective.
Testing was standardized for each sample or examination. Data were presented as means ± standard deviations ( ). Change in each outcome variables between pre- and post-treatment was assessed using paired T-test. Bonferroni adjustment was made for multiple comparisons within each kind of outcome variables. An outcome variable was considered to be significant if p < 0.05/m, where m = number of comparisons made for each kind of outcomes. All statistical analyses were done using SPSS 13.0 statistical package. All statistical tests were two-sided and a p-value < 0.05 was considered statistically significant.
Patient # (n)
Patient # (n)
1.18 ± 0.247
1.19 ± 0.221
0.26 ± 0.073
0.25 ± 0.081
9.76 ± 3.079
8.09 ± 2.357
2.12 ± 0.808
1.92 ± 0.760
1.03 ± 0.792
1.05 ± 0.711
The frequency of exact diagnosis and confirmation of hereditary ataxias has risen in tandem with advances in genetic testing that define the different types and the locus of genotype variation, abnormalities within chromosomes and proteins. Mutational analysis can correlate with apoptosis, necrosis or degeneration of neurons in the cerebellum, brain stem, or spinal cord. The rate and quantity of atrophy and degeneration of neurons differ with the various types of hereditary ataxia and patients' ages. The pathological neuronal loss results in loss of cerebellospinal tracts and functional disorders. The physical manifestations translating to functional disability include unsteadiness in walking, wide-based steps, inability to heel walk, unsteadiness in standing or sitting, dependence on a walking frame, walking aid or wheel-chair, dysarthria and dysphagia. Despite genotypic variability, the phenotypic symptoms among patients are mostly similar, only differing in ages of onset or rates of progression. According to the iconography records of the FRDA subjects in this study, the onset of cerebellar atrophy is approximately five years before symptoms appear and the progression is in direct proportion to the atrophy ratio of cerebellum and spinal cord. Patients eventually develop severe functional impairment of swallowing, loss of locomotor capacity and even death due to respiratory muscle paralysis or pulmonary infection.
Prior studies attempt to treat neurodegenerative diseases with human embryonic olfactory ensheathing cell  and neural stem cell [17, 23] transplantation. However, there are no publications documenting systematic study of hereditary ataxia treatment with CBMCs. Based on our clinical experience, the short-term effect of CBMC transplantation combined with rehabilitation training on equilibrium function treating hereditary ataxia was significant. After receiving one treatment course, the patients were evaluated by physicians and therapists using BBS, a validated functional scale that measures the ability to walk, balance while standing and other activities of daily living for ataxia patients .
The average duration of symptoms of the subjects enrolled was over 10 years, and therefore, most received equilibrium function training without significant improvements prior to CBMC treatment. One of the patients with SCA6 who needed complete support while walking and had abnormal Romberg sign (+), heel-knee-tibia test (+) and heel test (+) at baseline, subjectively felt marked improvements immediately after the CBMC transplantation and could objectively walk without support. He also finished the heel test after three CBMC transplantations. In addition, this subject's condition remained stable three years after the treatment according to the follow up examinations.
One family from Saskatchewan, Canada had 32 individuals with confirmed SCA2 from 80 tested family members spanning four generations. Sixteen members of the family had already expired directly from the disease or complications stemming from it. Six male siblings or children from this family participated in the trial. The symptoms in the third generation were relatively mild and all were able to move with support. However, in the fourth generation, symptoms started by age 16 years old. Moreover, all signs and symptoms continued to progress. By age 19, when one fourth generation family member participated, he had already lost his ability to walk. After one course of treatment, his BBS score rose from 26/56 to 43/56. Unfortunately, because of geographical distance, it was impossible to provide long-term follow-up details on all patients who received the treatment.
The interval between baseline and post-treatment of serum IgG, IgA, IgM, C3, C4 and T cell subsets tests, as per protocol, was about a month. IgG, IgA, total T cells and CD3+CD4 T cells decreased significantly after treatment (P < 0.01). Although there are numerous proportioned mechanisms of action, one possibility is that CBMCs exercise broad inhibitory action on cellular and humoral immunity. One limitation of the study was that some patients received treatment with CBMC for 20 days in total, whereas others received up to 42 days in total. There were no significant differences in immunological profiles or clinical responses between the 20 to 42 day treatment groups, however this is a question that may be addressed in future studies.
Cord blood derived cells are being investigated in a myriad of preclinical disease models [18, 19, 24, 25]. The safety of CBMC transplantation has been investigated in several human clinical trials with neurodegenerative conditions and has not revealed any severe adverse events, immune reactivity or Graft-versus-host-disease [16, 26, 27]. The potential concern regarding GVHD induced by allogeneic cord blood administered in absence of immune suppression is mitigated by the fact that hundreds of administrations of allogeneic lymphocytes have been performed in women with recurrent spontaneous abortions as a method of immune modulation, without GVHD being observed . Mechanism studies suggest that multi-potent cells in the heterogeneous CBMC population may not only differentiate into osteoblasts, chondroblasts, adipocytes and neurons and astrocytes to act as a cell replacement source, but also produce antioxidants, several neurotrophic and angiogenic factors and modulate immune and inflammatory reaction [19, 28, 29]. Intravenously administered CBMCs enter brain, survive, migrate, improve functional recovery and reduce infarct volume in the middle cerebral artery occlusion rat stroke model through the action of anti-inflammatory, neuroprotection and neovascularization [30, 31]. Cord blood stem cells infusion into the systemic circulation of G93A mice, an amyotrophic lateral sclerosis (ALS) model, delayed disease progression for 2-3 weeks and increased lifespan of diseased mice by providing cell replacement and protection of motor neurons . Transplantation of hUCB cells into the spinal cord injury (SCI) rats most likely inhibits the apoptotic cascade which is followed by axonal remyelination, regeneration of the damaged neural tissues, potential restoration of blood flow to the damaged area by neovascularization, and modulation of the immune/inflammatory response to the injury [33, 34]. Accordingly, these multiple restorative and protective effects from CBMC grafts may act in harmony to exert therapeutic benefits for hereditary ataxias, but the exact mechanism of action still remains unconfirmed.
This open label single dose treatment case series using CBMC transplantation in 30 subjects with hereditary ataxia demonstrated statistically significant endpoints of functional and surrogate immune marker changes from baseline. In addition to the early effect seen in some subjects, the measured symptomatic improvements persisted throughout the period of the study, as noted with the follow-up data from a subset of subjects. These data suggest a potential treatment using CBMC transplantation for hereditary ataxia and possibly other neurodegenerative conditions involving the spinal cord or cerebellum. Based on the current data, further double-blind placebo controlled studies are warranted to validate the efficacy, safety and long-term effects.
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