Methylation of miR-34a, miR-34b/c, miR-124-1 and miR-203 in Ph-negative myeloproliferative neoplasms

Table 2 MSP primer sequences and reaction conditions

Gene	Forward primer (5' - 3')	Reverse primer (5' - 3')	MgCl₂/Tm/Cycles	Reference
miR-34a
M-MSP	GGGGATGAGGATTAGGATTTC	ACAAAACGCATAAAAACGACG	1.5 mM/58°C/35	[9]
U-MSP	GGGGATGAGGATTAGGATTTT	CAAACAAAACACATAAAAACAACA	1.5 mM/58°C/35
miR-34b/c
M-MSP	ATTCGTTTCGTTTCGCGTTCGTTTC	CGACTACAACTCCCGAACGATCCG	2.0 mM/58°C/35	[34, 35]
U-MSP	TTTTTATTTGTTTTGTTTTGTGTTTGTTTTG	CAACTACAACTCCCAAACAATCC	1.25 mM/56°C/38
miR-124-1
M-MSP	AAAGAGTTTTTGGAAGACGTC	AATAAAAAACGACGCGTATA	1.5 mM/55°C/35	[36]
U-MSP	AATAAAGAGTTTTTGGAAGATGTT	AAAAAAATAAAAAACAACACATATAC	2.0 mM/55°C/35
miR-203
M-MSP	GAGTATTTTCGGTTTAGACGAGAC	CCTTTTATACGACGCAACCG	1.5 mM/58°C/35	[37]
U-MSP	TTTGAGTATTTTTGGTTTAGATGAGAT	AACACCTTTTATACAACACAACCA	1.5 mM/58°C/35

Abbreviations: Tm, annealing temperature; M-MSP, MSP for the methylated allele; U-MSP, MSP for the unmethylated allele

ISSN: 1479-5876