From: Methylation of miR-34a, miR-34b/c, miR-124-1 and miR-203 in Ph-negative myeloproliferative neoplasms
Gene | Forward primer (5' - 3') | Reverse primer (5' - 3') | MgCl2/Tm/Cycles | Reference |
---|---|---|---|---|
miR-34a | Â | Â | Â | Â |
   M-MSP | GGGGATGAGGATTAGGATTTC | ACAAAACGCATAAAAACGACG | 1.5 mM/58°C/35 | [9] |
   U-MSP | GGGGATGAGGATTAGGATTTT | CAAACAAAACACATAAAAACAACA | 1.5 mM/58°C/35 |  |
miR-34b/c | Â | Â | Â | Â |
   M-MSP | ATTCGTTTCGTTTCGCGTTCGTTTC | CGACTACAACTCCCGAACGATCCG | 2.0 mM/58°C/35 | |
   U-MSP | TTTTTATTTGTTTTGTTTTGTGTTTGTTTTG | CAACTACAACTCCCAAACAATCC | 1.25 mM/56°C/38 |  |
miR-124-1 | Â | Â | Â | Â |
   M-MSP | AAAGAGTTTTTGGAAGACGTC | AATAAAAAACGACGCGTATA | 1.5 mM/55°C/35 | [36] |
   U-MSP | AATAAAGAGTTTTTGGAAGATGTT | AAAAAAATAAAAAACAACACATATAC | 2.0 mM/55°C/35 |  |
miR-203 | Â | Â | Â | Â |
   M-MSP | GAGTATTTTCGGTTTAGACGAGAC | CCTTTTATACGACGCAACCG | 1.5 mM/58°C/35 | [37] |
   U-MSP | TTTGAGTATTTTTGGTTTAGATGAGAT | AACACCTTTTATACAACACAACCA | 1.5 mM/58°C/35 |  |