Multiplex serum biomarker assessments: technical and biostatistical issues

Butterfield, Lisa H; Potter, Douglas M; Kirkwood, John M

doi:10.1186/1479-5876-9-173

Journal of Translational Medicine

Table 1 Old patient Serum Samples

From: Multiplex serum biomarker assessments: technical and biostatistical issues

Sample	Date Drawn	Draw Date	Assay Date	IL-4 pg/mL	IL-6 pg/mL	IL-8 pg/mL	IL-10 pg/mL	TNF-α pg/mL	IFN-g pg/mL	GM-CSF pg/mL	IP-10 pg/mL	MIG pg/mL	MCP-1 pg/mL
patient 1	6/2/2005	6/1/2005	10/31/2005	< 5	217	64	< 10	38	29	< 15	1339	62	10145
			8/11/2010	25	106	353	6	< 10	28	< 15	1214	48	> 7200
			8/11/2010	16	98	370	6	< 10	29	< 15	1195	42	> 7200
patient 2	6/2/2005	6/1/2005	10/31/2005	< 5	41	24	< 10	19	23	< 15	> 2800	130	2725
			8/11/2010	13	20	132	7	< 10	24	< 15	> 9600	164	3149
			8/11/2010	13	22	144	7	< 10	26	< 15	> 9600	162	2989
patient 3	6/2/2005	6/1/2005	10/31/2005	< 5	13	23	< 10	7	< 14	< 15	55	62	384
			8/11/2010	5	5	125	5	< 10	< 5	< 15	83	90	419
			8/11/2010	7	6	151	5	< 10	< 5	< 15	83	98	455
patient 4	7/30/2005	7/30/2005	11/1/2005	32	49	17	17	83	95	173	64	241	394
			2/2/2010	47	17	75	20	21	63	132	87	209	633
			5/13/2010	42	14	73	20	20	51	124	84	153	554
			5/13/2010	42	15	69	22	21	62	133	84	154	518
			8/11/2010	55	21	89	27	28	118	89	90	213	140
			8/11/2010	47	23	87	33	32	144	105	90	209	128
patient 5	8/9/2005	8/9/2005	11/1/2005	12	2199	266	43	100	178	< 15	> 2800	407	> 17800
			2/2/2010	6	1105	1469	< 14	17	159	37	2178	374	11991
			5/13/2010	13	949	1494	< 14	16	140	36	2476	313	10275
			5/13/2010	12	971	1428	< 14	13	121	24	2233	300	9045
patient 6	8/9/2005	8/9/2005	11/1/2005	14	592	171	28	52	229	< 15	> 2800	2586	10705
			2/2/2010	10	350	1016	< 14	< 10	198	37	2176	2039	11703
			5/13/2010	13	275	971	< 14	< 10	155	18	> 2980	2276	9492
			5/13/2010	< 5	270	968	< 14	< 10	160	11	> 2980	2294	10581
patient 7	8/15/2005	8/15/2005	11/1/2005	< 5	19	47	< 5	13	< 14	< 15	285	40	2565
			5/13/2010	8	24	242	< 14	< 10	31	< 15	445	25	5453
			5/13/2010	< 5	20	234	< 14	< 10	27	< 15	360	25	5088
patient 8	8/20/2005	8/20/2005	11/1/2005	36	48	17	17	80	111	197	27	182	379
			2/2/2010	46	17	99	23	23	78	146	45	172	614
			5/13/2010	48	16	87	26	25	84	175	40	128	550
			5/13/2010	50	15	84	22	21	70	151	41	117	544
			8/11/2010	33	15	89	23	21	103	85	47	136	119
			8/11/2010	49	21	107	29	30	136	95	48	150	125
patient 9	9/15/2005	9/15/2005	11/1/2005	42	67	21	11	72	122	208	275	383	1119
			5/13/2010	77	22	78	28	36	80	224	346	328	2162
			5/13/2010	80	22	76	27	34	87	233	347	306	2270
patient 10	9/19/2005	9/19/2005	2/17/2006	< 5	19	45	< 5	13	< 7	< 15	183	42	1656
			5/13/2010	8	24	287	< 14	< 10	26	< 15	350	21	5509
			5/13/2010	8	22	290	< 14	< 10	29	< 15	347	17	5021
patient 11	9/16/2005	9/22/2005	11/1/2005	48	94	2675	24	142	135	201	902	419	10026
			5/13/2010	85	37	12663	29	39	114	272	988	300	13807
			5/13/2010	77	37	13690	27	34	114	266	1337	285	14377
			8/11/2010	93	41	14814	26	59	72	219	716	340	> 7200
			8/11/2010	110	48	12823	30	58	80	238	793	364	> 7200
patient 12	9/28/2005	9/28/2005	2/17/2006	62	92	27	39	82	183	328	51	270	436
			2/2/2010	46	22	92	36	20	118	222	88	200	909
			5/13/2010	43	18	75	35	20	109	221	76	125	741
			5/13/2010	34	18	88	35	22	124	208	78	165	755
patient 13	10/6/2005	10/5/2005	11/1/2005	28	67	51	8	80	77	131	877	326	6818
			8/11/2010	83	42	342	29	55	64	225	725	332	> 7200
			8/11/2010	116	44	335	33	72	79	228	802	356	7345
patient 14	10/7/2005	10/6/2005	11/1/2005	42	67	24	18	66	122	156	353	711	1266
			8/11/2010	89	39	118	35	66	59	237	314	906	1088
			8/11/2010	70	35	114	30	53	52	189	328	891	1057
patient 15	10/12/2005	---	2/17/2006	51	76	26	32	86	180	276	63	257	395
			2/2/2010	38	21	113	48	23	146	255	107	245	801
			5/13/2010	35	17	95	44	23	116	205	89	170	601
			5/13/2010	28	15	100	42	20	107	224	82	168	576
patient 16	10/17/2005	---	2/17/2006	< 5	54	60	< 5	34	< 7	< 15	434	55	3950
			5/13/2010	5	41	368	< 14	< 10	39	< 15	780	25	10159
			5/13/2010	11	43	373	< 14	< 10	39	< 15	846	23	10552
			8/11/2010	21	52	402	6	< 10	6	15	464	35	4616
			8/11/2010	8	29	391	< 5	< 10	< 5	< 15	465	35	4777
patient 17	11/3/2005	11/3/2005	2/17/2006	23	52	11	< 5	49	75	111	155	283	686
			5/13/2010	72	23	57	24	31	67	221	198	288	1734
			5/13/2010	17	9	27	< 14	< 10	14	53	186	218	1706
			8/11/2010	90	36	63	28	60	67	222	203	370	757
			8/11/2010	85	30	60	24	52	56	213	202	325	705
patient 18	11/16/2005	--	2/17/2006	7	18	14	< 5	20	29	44	41	79	327
			2/2/2010	16	15	74	< 14	< 10	34	51	59	93	807
			5/13/2010	10	12	71	< 14	< 10	20	24	57	50	611
			5/13/2010	8	10	72	< 14	< 10	24	30	52	48	617
patient 19	11/16/2005	---	2/17/2006	10	48	18	< 5	41	39	67	144	96	1143
			2/2/2010	16	30	116	< 14	< 10	43	75	248	108	2812
			5/13/2010	13	22	100	< 14	10	35	67	202	75	2101
			5/13/2010	11	24	108	< 14	< 10	38	61	220	67	2304
patient 20	12/8/2005	12/8/2005	2/17/2006	27	31	10	< 5	62	73	149	35	162	452
			2/2/2010	49	17	61	24	24	89	146	60	162	1422
			5/13/2010	43	12	48	19	24	70	137	52	108	1114
			5/13/2010	41	12	50	21	21	77	137	55	106	1234
patient 21	12/12/2005	12/9/2005	2/17/2006	< 5	24	41	< 5	16	< 7	< 15	537	35	1236
			5/13/2010	8	22	235	< 14	< 10	29	< 15	682	25	3153
			5/13/2010	10	23	233	< 14	< 10	27	< 15	789	25	3457
			8/11/2010	14	29	235	11	< 10	7	< 15	518	34	1423
			8/11/2010	13	25	238	11	< 10	6	< 15	546	39	1322
patient 22	1/26/2006	1/25/2006	2/17/2006	8	30	4	< 5	24	24	40	706	216	24
			8/11/2010	47	22	44	17	45	37	196	332	283	888
			8/11/2010	56	26	47	19	53	42	223	318	283	959
patient 23	1/26/2006	1/25/2006	2/17/2006	8	56	68	< 5	75	20	< 15	8705	266	75
			8/11/2010	75	3953	534	19	56	50	202	650	339	> 7200
			8/11/2010	76	4542	525	19	58	48	210	695	318	> 7200
					223

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ISSN: 1479-5876

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